土白术药材中(6E,12E)-十四癸二烯-8,0-二炔-1,3-二乙酸酯和芹烷二烯酮的定性定量方法研究

马斯怡; 侴桂新

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土白术药材中(6E,12E)-十四癸二烯-8,0-二炔-1,3-二乙酸酯和芹烷二烯酮的定性定量方法研究

实验研究 | 更新时间：2022-08-31

- 土白术药材中(6E,12E)-十四癸二烯-8,0-二炔-1,3-二乙酸酯和芹烷二烯酮的定性定量方法研究
- Qualitative and quantitative research of (6E,12E)- Tetradecadiene-8,0-diyne-1,3- dioldiacetate and Selina-4(14), 7(11)-dien-8-one in Atractylodis Japonicae Rhizoma
- 上海中医药杂志 2016年50卷第6期页码：94-98
- 作者机构：
  
  1. 上海中医药大学中药学院,上海,201203
  2. 上海中药标准化研究中心,上海,201203
- 作者简介：
- 基金信息：
  
  国家药典委员会“国家药品标准提高暨2015版药典科研任务”
- DOI：
  中图分类号：
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马斯怡, 侴桂新. 土白术药材中(6E,12E)-十四癸二烯-8,0-二炔-1,3-二乙酸酯和芹烷二烯酮的定性定量方法研究[J]. 上海中医药杂志, 2016,50(6):94-98.

MA Si-yi, CHOU Gui-xin. Qualitative and quantitative research of (6E,12E)- Tetradecadiene-8,0-diyne-1,3- dioldiacetate and Selina-4(14), 7(11)-dien-8-one in Atractylodis Japonicae Rhizoma[J]. Shanghai Journal of Traditional Chinese Medicine, 2016,50(6):94-98.
马斯怡, 侴桂新. 土白术药材中(6E,12E)-十四癸二烯-8,0-二炔-1,3-二乙酸酯和芹烷二烯酮的定性定量方法研究[J]. 上海中医药杂志, 2016,50(6):94-98. DOI：

MA Si-yi, CHOU Gui-xin. Qualitative and quantitative research of (6E,12E)- Tetradecadiene-8,0-diyne-1,3- dioldiacetate and Selina-4(14), 7(11)-dien-8-one in Atractylodis Japonicae Rhizoma[J]. Shanghai Journal of Traditional Chinese Medicine, 2016,50(6):94-98. DOI：

摘要

目的：建立土白术药材中(6E,12E)-十四癸二烯-8,0-二炔-1,3-二乙酸酯(TDEYA)和芹烷二烯酮两种化学成分的定性定量方法。方法：采用薄层色谱法和高效液相色谱法。薄层色谱采用G254薄层硅胶预制板,以石油醚-乙酸乙酯(10∶1)为展开剂,置紫外光(254 nm)下检视。高效液相采用ZORBAX SB-C,18,色谱柱(250 mm×4.6 mm,5 μm),流动相为甲醇-1%甲酸溶液(67∶33),检测波长为254 nm,流速1.0 ml/min；柱温30 ℃；进样量10 μl。结果：TDEYA和芹烷二烯酮在薄层色谱和高效液相色谱上均获得良好的分离,可作为土白术药材的定性定量指标。结论：本法操作简单,结果准确,重现性好,可为评价土白术药材的质量标准提供检测方法。

Abstract

Objective:To use the polytene component (6E,12E)- Tetradecadiene-8,0-diyne-1,3-dioldiacetate (TDEYA) and sesquiterpene component selina-4(14),7(11)-dien-8-one in Atractylodis Japonicae Rhizoma as the control chemicals to establish a TLC qualitative method and a HPLC quantitive method. MethodsTLC method:place samples and control chemicals on pre-coated TLC plate (SIL G-25 UV254); use petroleum ether-ethyl acetate=10:1 as development solvent. The TCL plate was observed under 254 nm UV lamp. HPLC method:ZORBAX SB-C,18, (250 mm×4.6 mm, 5 μm); mobile phase:methanol-1% formic acid solution (67∶33); samples were detected under 254 nm ultraviolet lamp; flow rate:1.0 ml/min; column temperature:30 ℃; injection amount:10 μl. Results:The polytene component TDEYA and sesquiterpene component selina-4(14), 7(11)- dien-8-one were separated well by TLC and HPLC. This method could be used as the qualitative and quantitative methods of evaluating Atractylodis Japonicae Rhizoma. Conclusion: This method is simple in operation, accurate in results and good in reappearance, and can be used as the detection method for evaluating the quality of Atractylodis Japonicae Rhizoma.

关键词

土白术(6E12E)-十四癸二烯-80-二炔-13-二乙酸酯芹烷二烯酮薄层色谱高效液相色谱

Keywords

Atractylodis Japonicae Rhizoma(6E12E)-Tetradecadiene-80-diyne-13- dioldiacetateselina-4(14) 7(11)- dien-8-oneTLCHPLC

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