Objective:To observe the effects of Warming Kidney and Tonifying Spleen interventions on IBS-D rats’ colonic mucosa, ileocecal CCK and MOT to probe into the underlying mechanism from the gastrointestinal hormone perspective  Methods:48 rats were randomly divided into normal group, model group, Warming Kidney and Tonifying Spleen high, middle and low dose groups, and control group, 8 in each group. Referencing AL-Chaer modeling,made a duplicate IBS-D rat model. After that, the treatment groups were treated with high dose, middle dose and low dose Decocting-free Warming Kidney and Tonifying Spleen formula decoction, while the control group was treated with Decocting-free “Sishen Pill”, and the model group was treated with equal quantity of normal saline irrigation, 2 weeks for each group. The general situation of rats in each group was observed, and immunohistochemistry analysis was employed to observe the changes in their colonic mucosa,ileocecal CCK and MOT  Results:After the treatment, the general situation of rats in each group became better to various degree, and the situation of rats in the Warming Kidney and Tonifying Spleen high and middle groups was better than that of the low dose and control groups. Compared with normal group, the expression of colonic mucosa, ileocecal CCK and MOT in the rats in model group increased , and there was significant statistic difference(P<0.05). Compared with model group after the treatment, the expression of colonic mucosa, ileocecal CCK and MOT in the rats in the Warming Kidney and Tonifying Spleen high, middle and control groups decreased. Compared with low dose group, the expression of colonic mucosa, ileocecal CCK and MOT in the rats in the Warming Kidney and Tonifying Spleen high, middle groups decreased, and there was significant statistic difference(P<0.05)  Conclusion:Warming Kidney and Tonifying Spleen interventions proved to be effective to reduce the quantity of colonic mucosa and the amount of released ileocecal CCK and MOT, which in turn reduces the intestinal sensitivity and also accelerate the degradation of CCK and MOT present in their body.