Objective:To explore the effects of “Huangqi Guizhi Wuwu Decoction”(HGWD)on myocardial cell in ischemia reperfusion injury to study its protective function  Methods:An anoxia/reoxygenation model of myocardial cells in Wistar rats was established.The experiment was divided into 6 groups:normal group, model group, control group,HGWD of high-, medium-and low-dose groups. Normal group and model group were given saline by gavage; control group was given same amount of Compound Danshen dripping pill solution by gavage(0.075 g·kg-1·d-1); HGWD groups were respectively given equivalent amount of high(9.0 g·kg-1·d-1),medium (4.5 g·kg-1·d-1) and low(2.25 g·kg-1·d-1) dose of HGWD; 3 days after intragastric administration,draw blood from abdominal aorta to prepare drug-containing serum. The use of drugs containing different concentrations of hypoxic preconditioning 1h medium, hypoxia reoxygenation injury model was set up for each experimental group. The CK activity, NO content, SOD activity and MDA content were observed; Realtime PCR was used to detect the expressions of Bcl-2 and Bax mRNA; Western blot was adopted to detect the expressions of Bcl-2 and Bax protein  Results:“Huangqi Guizhi Wuwu Decoction” could reduce CK activity, NO and MDA contents, and increase SOD activity in myocardial cells with hypoxia reoxygenation injury; it could also reduce theexpressions of Baxprotein and mRNA, and increase theexpressions of Bcl-2 mRNA and protein  Conclusion:“Huangqi Guizhi Wuwu Decoction” exert protective effects on myocardial cells with ischemia reperfusion injury.