Objective:To observe the clinical efficacy of minimally invasive stitch in the treatment of low and high simple anal fistula  Methods:One hundred cases with low and high simple anal fistula were divided into test group and control group randomly, 50 cases ineach group. Test group was treated by minimally invasive stitch, and control group was treated with traditional low and high simple anal fistula otomy. Observe the clinical efficacy, healing time, postoperative pain, anus functions  Results:The total effective rates were 100% in the two groups. The healing time, postoperative pain were reduced and anus functions was protected effectively by minimally invasive stitch in test group which better than control group, with remarkable difference between the two groups (P<0.05)  Conclusion:Both treatments of minimally invasive stitch and anal fistula otomy are effective surgical method for anal fistula, but minimally invasive stitch has advantages including shorten the healing time, relieve postoperative pain and protect the anal function better.