Objective:To observe effects of arctigenin on expression of joint chondrocytes proliferation and Ⅱ Collagen  Methods:Articlular chondrocytes were isolated by enzyme digestion method and chondrocyteⅠwere inoculated into 6 well culture plate and 96 well culture plate. The test was divided into blank group, low and high Concentration arctigenin groups. After the intervention measures for 48 hours in each group, the expression of chondrocytesⅡ Collagen were observed by SABC method and chondrocyte proliferation was tested by MTT and content of Ⅱ Collagen was tested by ELISA  Results:① Compared with the blank group, the quantity of chondrocytes and the positive staining potency of the low and high Concentration arctigenin groups were significantly increased, and the increasing range of high Concentration arctigenin group was higher than the low Concentration arctigenin group. ② Compared with the blank group,OD of Concentration and contents of Ⅱ Collagen in low and high Concentration arctigenin groups were significantly increased (P<0.05), and the increasing range of high Concentration arctigenin group was higher than the low Concentration arctigenin group (P<0.05)  Conclusion:Arctigenin can significantly up regulate the expression of articular chondrocytes proliferation and Ⅱ Collagen and can maintain Concentration phaenotype which can be used in the treatment of osteoarthritis effectively.