Objective:To explore the effects of “Shaoyao Gancao Decoction” on excitatory/inhibitory amino acid level in patients with spastic hemiplegia after cerebral ischemia-reperfusion (CI/RP) and to explore its mechanism  Methods:Ninty-four model SD rats were randomly divided into the model group, Shaoyao Gancao group, Shaoyao group, Gancao group, baclofen group and sham-operated group. Implementing ischemia reperfusion after 2 hours of right middle cerebral artery embolism surgery was used as research model, to screen rats that had obvious hemiplegia with spasm as the research subjects. Intervention implemented among groups after 24 hours of CI/RP, and rats were treated for 9 days consciously. The attitudinal reflex, muscular tension and levels of levels of glutamic acid (Glu), aspartic acid (Asp), γ-aminobutyric acid (GABA) and glycine (Gly) in brain tissues and serum were observed  Results:①Scores of postural reflex assessment of rats were improved significantly in the “Shaoyao Gancao Decoction” group and Shaoyao group (P<0.01, P<0.05). ② The musclar tension of rats was relieved significantly in the “Shaoyao Gancao Decoction” group and baclofen group (P<0.05). ③ Gly contents in brain tissue of the “Shaoyao Gancao Decoction” group was lower than that of the Gancao group (P<0.05). ④The GABA content in brain tissue of the baclofen group was obviously lower than that of the model group (P<0.01). ⑤Serum level of Glu and Asp of the “Shaoyao Gancao Decoction” group, Shaoyao group and the baclofen group were significantly lower than that of the model group (P<0.05, P<0.01). ⑥The serum Gly level of the Gancao group was obviously higher than that of the “Shaoyao Gancao Decoction” and the Shaoyao group (P<0.05)  Conclusion:“Shaoyao Gancao Decoction” can improve the postural reflex assessment scores and the degree of spasm in rats after CI/RP. Its mechanism might be related to the decrease of excitatory amino acids content in serum and Gly in brain tissues of rats with hemiplegia and spasm after CI/RP.