Objective: To observe the clinical effect of Chinese medicine enema on serum beta-2 microglobulin (β,2,-MG) in patients with chronic kidney disease (CKD) at stage 3~4  Methods: Forty subjects with CKD3~4 were randomly divided into enema group and control group, with 20 subjects in each group. Control group was treated with conventional western medicine, and enema group was added with Chinese medicine, with the course of 10 weeks. The clinical efficacy and changes of Scr, BUN and β,2,-MG were observed before and after treatment in both groups  Results: The total effective rate was 75.0% in the control group, and was 90.0% in the enema group; with significant difference between the two groups (P,<,0.05). After treatment, no significant difference was found in the levels of Scr, BUN and β,2,-MG in the control group (P,>,0.05); the levels of Scr, BUN and β,2,-MG in the enema group were decreased markedly (P,<,0.05), and the levels of the enema group was lower than that of the control group (P,<,0.05)  Conclusion: Chinese medicine enema therapy can improve the clinical efficacy and lower the β,2,-MG level in patients with CDK3~4 stage.