Objective:To investigate and compare the protective effect of two kinds of aqueous extractive of Penis et Testis Cervi against cisplatin(CDDP)-induced acute kidney injury in mice and discuss the possible mechanism. Methods64 mice were evenly and randomly divided into the control group,CDDP model group, aqueous extract of penis cervi of Cervus nippon and Cervus elaphus with low-,mid- and high- dose(100,0,400 mg/kg respectively)groups,8 mice in each group. The different groups of Penis et Testis Cervi were intragastrical administrated with corresponding aqueous extract for 7 days, the mice(except the control group)were intraperitoneally injected with CDDP(20 μg/g)to induce acute kidney injury on fifth day 1h after administration. After modeling 48 h, the index level of renal function in serum and kidney tissue was measured and the pathological change was observed by HE staining.  Results:Compared with CDDP model group,the groups treated with two kinds of aqueous extract of Penis et Testis Cervi against with different dose were all improved the index level of renal function and renal damage,and the effect of penis cervi in Cervus nippon was better than Cervus elaphus. It showed the most significant effect in the group treated with penis cervi of Cervus nippon at high dose,which the contents of BUN,CRE and MDA significantly decreased(P<0.01)and the activity of SOD and GSH significantly increased(P<0.01). It obviously improved the degeneration and necrosis of renal tubule,but not recovered.  Conclusion:These two kinds of aqueous extract of Penis et Testis Cervi effectively protected mice from the acute kidney injury induced by cisplatin. It may be closely related to cleaning out radicals and anti-oxidative stress.