最新刊期
期 8 ， 2022
- 关键词：SARS-CoV-2;COVID-19;Omicron variant;traditional Chinese medicine therapy;sequela;rehabilitation;expert consensus
- 52
- |
- 1226
- |
- 0
发布时间：2022-08-12 - 关键词：SARS-CoV-2;COVID-19;Omicron variant;children;traditional Chinese medicine therapy;expert consensus
- 36
- |
- 629
- |
- 0
发布时间：2022-08-12 - 摘要：ObjectiveTo explore the implementation pattern of “three zones and two passages” alteration and localized transformation， organization and coordination management required for nosocomial infection prevention and control during the emergency transformation of a tertiary general hospital into a designated hospital for COVID-19.MethodsWith the system methodology of “Wuli⁃Shili⁃Renli （WSR）” and the goal of “zero infection among medical and nursing staff”， the characteristics of current hospital zone division were carefully studied， and the scheme of real-time data dynamic management and effective allocation of emergency resources was constructed from the aspects of reasonable zone division and passage planning， clear signs of nosocomial infection prevention and control， establishment of nosocomial infection prevention and control process， and three-zone linkage team-building for nosocomial infection prevention and control.ResultsWith the intervention of a series of nosocomial infection prevention and control measures， the prevention and control effect was satisfactory and zero nosocomial infection was achieved.ConclusionThe practice and exploration of WSR approach for nosocomial infection prevention and control in a tertiary general hospital converted to a designated hospital for COVID-19 can be used as a reference and a paradigm for dealing with public health emergencies such as sudden unexpected infectious diseases.关键词：SARS-CoV-2;COVID-19;Omicron variant;designated hospital;nosocomial infection;infection prevention and control
- 19
- |
- 89
- |
- 0
发布时间：2022-08-12 - 摘要：In order to improve the efficacy of treatment for elderly COVID-19 patients in Shanghai， a team of experts analyzed the characteristics of Omicron variant and clinical characteristics of elderly COVID-19 patients， drew on experience of current treatment， revised the treatment protocol based on Diagnosis and Treatment Protocol for COVID-19 Patients （Tentative 9th Edition） and formed Expert Consensus on Traditional Chinese Medicine Treatment for Elderly COVID-19 Patients in Shanghai （2nd Edition） to guide clinical practice. The 2nd edition of expert consensus highlighted the following points： ①Pay attention to elderly patients with underlying diseases， choose priority from the newly contracted COVID-19 infection and the underlying diseases， and implement a timely multidisciplinary approach to diagnosis and treatment； ②Due to the insidious and nonspecific onset of symptoms in elderly patients， prognostic indicators of both clinical symptoms and laboratory tests should be refined to facilitate early detection of disease progression； ③Supplement TCM treatment principles and basic herbal prescriptions for moderate， severe and critical cases of elderly COVID-19 patients， as well as recommend the use of TCM emergency injections； ④Revise the TCM therapeutic scheme for high fever that does not subside in elderly COVID-19 patients； ⑤Supplement a TCM therapeutic scheme for unconsciousness in elderly COVID-19 patients； ⑥Supplement a TCM therapeutic scheme for constipation of “deficiency syndrome” in elderly COVID-19 patients； ⑦Update a TCM therapeutic scheme for filthy and greasy tongue coating caused by pathogen hidden in interpleuro-diaphramatic space in elderly COVID-19 patients； ⑧Update prescriptions of TCM injection for exacerbated infiltrating on pulmonary radiography in elderly COVID-19 patients； ⑨Recommend a TCM therapeutic scheme for sticky phlegm hard to be expectorated in elderly COVID-19 patients； ⑩Recommend different TCM treatment regimens for elderly patients with different deficiency conditions； ⑪Separate TCM treatment protocols for elderly COVID-19 patients with cardiovascular， neurological or renal complications.关键词：SARS-CoV-2;Omicron variant;COVID-19;elderly;traditional Chinese medicine therapy;expert consensus
- 30
- |
- 379
- |
- 0
发布时间：2022-08-12 - 摘要：In this article we analyzed the pathogenic characteristics and treatment strategies of COVID-19 infection complicated with underlying diseases in the elderly by introducing 2 proved cases. Elderly patients with COVID-19 are identified with pathogenesis of dampness， heat， toxicity， stasis and deficiency， and the pathogenesis of deficiency and stasis is the most prominent. The deficiency of vital qi exists throughout the disease course. Traditional Chinese medicine （TCM） treatment principles for elderly patients with COVID-19 include： early intervention， integration of pathogen-eliminating and vital-qi strengthening， and application of treatment methods to guide pathogens out， for instance， the method of dispersing lungs and unblocking bowels， the method of clearing heat and resolving dampness， and the method of supplementing and strengthening vital qi， etc. Additionally， attention should be paid to the treatment of underlying diseases. Treat both the newly contracted infection and persistent diseases， but one need to distinguish the priorities， and treat accordingly and flexibly.关键词：COVID-19;SARS-CoV-2;Omicron variant;epidemic;elderly;eliminating pathogen and strengthening vital qi;clinical experience
- 24
- |
- 413
- |
- 0
发布时间：2022-08-12 - 摘要：This paper has summarized the main points of modern medicine knowledge of epidemic hemorrhagic fever and the basic understanding， syndrome differentiation and treatment of the disease in traditional Chinese medicine. By reviewing the historical changes in the understanding of febrile diseases， this paper aims to discuss how to identify and understand febrile diseases from the modern medicine perspective. Additionally， specific problems in the clinical diagnosis and treatment have been contemplated based on the comparisons between epidemic hemorrhagic fever in real life and hemorrhagic fever in historical records.关键词：epidemic hemorrhagic fever;febrile disease;comparison between modern and ancient medicine
- 19
- |
- 73
- |
- 0
发布时间：2022-08-12 - 摘要：ObjectiveTo study and analyze the medication rule of soothing the liver and clearing heat in the treatment of comedomastitis by data mining technology.MethodsMedical records and prescriptions of the patients with comedomastitis（syndrome of heat stagnation in liver meridian） were collected in Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from January 2019 to December 2021， and data mining analysis（BK algorithm， complex system entropy network） was performed using TCMNPAS， a TCM network pharmacology analysis system.Results①A total of 28 530 prescriptions were analyzed， involving 526 traditional Chinese herbal medicines， with a cumulative frequency of 416 463 times. ②There were 24 traditional Chinese herbal medicines with a frequency of more than 20%， and 9 traditional Chinese herbal medicines with a frequency of more than 10 000 （>35%） including Chinese herbal medicines such as （Taraxaci Herba， Bupleuri Radix， Gleditsiae Spina， raw Crataegi Fructus， Angelicae Sinensis Radix， Sinapis Semen， Curcumae Rhizoma， Scutellariae Radix， Salviae Miltiorrhizae Radix Et Rhizoma）. ③The top 30 traditional Chinese medicines were mainly Taraxaci Herba， Bupleuri Radix， Gleditsiae Spina， and so on. The top 30 core drug pairs of correlation value were Atractylodis Macrocephalae Rhizoma -Poria， Scutellariae Radix - Bupleuri Radix， Semiaquilegiae Radix - Smilacis Glabrae Rhizoma， and so on. ④After analysis， four groups of potential “core prescriptions” were found， among which the one with the highest confidence was Gleditsiae Spina， Scutellariae Radix， Taraxaci Herba， Angelicae Sinensis Radix， Bupleuri Radix， Curcumae Rhizoma， Sinapis Semen， Fritillariae Thunbergii Bulbus， Glycyrrhizae Radix Et Rhizoma and Lonicerae Japonicae Flos.ConclusionsThe method of soothing the liver and clearing heat is one of the classic internal treatment rules of TCM for comedomastitis， which often includs the Chinese herbal medicines such as Bupleuri Radix， Citri Reticulatae Pericarpium， Curcumae Radix， Taraxaci Herba， Scutellariae Radix， Oldenlandia， Glycyrrhizae Radix Et Rhizoma， Prunellae Spica，Lonice Raejaponicae Caulis and Smilacis Glabrae Rhizoma and so on. According to the patient’s condition， it can be combined with Chinese herbal medicines such as Angelicae Sinensis Radix， Curcumae Rhizoma， Salviae Miltiorrhizae Radix Et Rhizoma for activating blood and resolving stasis， Sinapis Semen， Cervi Cornu， Cinnamomi Cortex and Zingiberis Rhizoma Praeparatum for warming yang to dissipate bind， Fritillariae Thunbergii Bulbus， Atractylodis Macrocephalae Rhizoma， Citri Reticulatae Pericarpium and Pinelliae Rhizoma for resolving phlegm and dissipating bind， and Gleditsiae Spina， Astragali Radix for subduing swelling and expelling toxin on the basis of the internal treatment principle of soothing the liver and clearing heat.关键词：comedomastitis;plasma cell mastitis;soothing the liver and clearing heat;data mining;medication rules;traditional Chinese herbal medicine research
- 9
- |
- 323
- |
- 0
发布时间：2022-08-12 - 摘要：ObjectiveTo investigate the distribution characteristics of traditional Chinese medicine syndrome elements in patients with cervical high risk human papillomavirus （HR-HPV） infection， in order to provide the objective evidence for its syndrome differentiation and treatment.MethodsThe method of epidemiological study was applied. Four diagnoses and its related information of 210 patients with cervical HR-HPV infection were collected， and the related syndrome essential factors were extracted by factor analysis method.ResultsA total of 14 common factors were extracted by factor analysis， and the cumulative variance contribution rate was 75.99%. The results showed that， the main disease location syndrome elements of patients with cervical HR-HPV infection were uterus， spleen， kidney， liver and heart， and the main disease nature syndrome elements were dampness， heat， yang deficiency， qi deficiency， qi stagnation， yin deficiency and blood stasis.ConclusionThe results of factor analysis in this study reflect the main characteristics of the syndrome elements of cervical HR-HPV infection to a certain extent， and have a certain reference value for the syndrome study of this disease.关键词：human papillomavirus;cervical carcinoma;factor analysis;syndrome element;treatment based on syndrome differentiation
- 12
- |
- 217
- |
- 0
发布时间：2022-08-12 - 摘要：According to the literature and history materials and clinical data， the clinical experience was sorted out of Gu's surgery school in diagnosis and treatment of breast disease. It is considered that Gu's surgical school attaches importance to the diagnostic and therapeutic approaches of the combination of the unique location and pathogenesis of the disease， the combination of internal and external pathogenesis， the changes of yin and yang， and pays attention to the smooth qi movement. In the treatment of breast disease，Gu's surgical school emphasizes recuperation of the patients when attacking pathogenic factors and resolving phlegm， the combination of internal therapy and external therapy，and focuses on the regulation of thoroughfare vessel and conception vessel， spleen and stomach， and emotion.关键词：breast disease;Gu's surgery;inheritance of schools;traditional Chinese medicine therapy
- 20
- |
- 171
- |
- 0
发布时间：2022-08-12 - 摘要：In this article， Professor Liu Sheng’s experience in treating postoperative lymphedema by focusing on qi， blood and water in breast cancer patients has been introduced and his main idea comes from the theory of “unsmooth blood circulation resulting in water retention”. Pro. Liu believes that “qi-transformation and qi-blood circulation is everywhere inside the body”. Therefore， the dysfunction of qi-transformation and water metabolism is the key to the occurrence of postoperative lymphedema， and the core pathogenesis is “unsmooth blood circulation resulting in water retention”. In terms of treatment， Professor Liu advocates a balance between supporting the vital qi and dispelling the pathogenic qi. Specifically， the methods of tonifying the kidneys， strengthening the spleen， benefiting qi and nourishing the blood are adopted to strengthen the root， while the methods of removing blood stasis with drastic medicinals and expelling blood stasis， inducing diuresis to remove edema are used to dispel the pathogenic factors. One proven case is presented for exemplification.关键词：unsmooth blood circulation resulting in water retention;breast cancer;perioperative;lymphedema;traditional Chinese medicine therapy
- 12
- |
- 323
- |
- 0
发布时间：2022-08-12 - 摘要：The clinical experience of Professor Xue Xiaohong in treating acne mastoid carbuncle （AMC） with the internal administration of traditional Chinese herbal medicines （TCHMs） based on the method of warming yang and dredging collaterals and local minimally invasive surgery is introduced in this article. In the process of diagnosis and treatment， Prof. Xue emphasizes yin-yang based syndrome differentiation and the integration of internal and external treatment. As to the pathogenesis of acne mastoid carbuncle， yang deficiency is the root and phlegm coagulation is the tip. The long-term phlegm accumulation transforms into heat， presenting a syndrome of intermingling of cold and heat. As to the internal treatment， Xue focuses on warming-eliminating， warming-dredging and warming-invigorating； As to the external treatment， Xue emphasizes the importance of timing， advising to stop treatment once the disease in controlled. One proved case is presented.关键词：plasma cell mastitis;acne mastoid carbuncle;traditional Chinese herbal medicine;minimally invasive surgery;traditional Chinese medicine therapy;clinical experience
- 13
- |
- 194
- |
- 0
发布时间：2022-08-12 - 摘要：Through the reanalysis of the theory of triple energizer and pericardium， it was found that as one of the six fu-organs， the triple energizer had a certain structure and specific physiological functions. It was believed that the triple energizer should be an independent latent space surrounded by the membrane inside the body cavity. Through combing the theory of the pericardium- triple energizer -membrane system， it was proposed that the pericardium should govern membrane， the external and internal relations between the pericardium and the triple energizer， realize the functional interconnection through the membrane and the meridian system， and the pathological mutual influence， therefore guiding the clinical practice.And the treatment and prescription of pericardium- triple energizer -membrane system diseases were been explained.关键词：triple energizer;pericardium;membrane;pericardium-triple energizer-membrane system;treatment;prescription;COVID-19
- 12
- |
- 239
- |
- 0
发布时间：2022-08-12 - 摘要：Ye Tianshi first put forward the theory of “liver being the congenital dependence of women” in Case Records as Guidance of Clinical Practice and emphasized the importance of liver to women. The theory is an important guidance on the treatment of gynecological diseases in later generations. Liver stores blood， governs conveyance and dispersion， and regulates the circulation of qi and blood in the human body. Liver has a close relationship with women’s physiology， pathology and the occurrence， development and outcome of diseases. This paper explains the important value of the theory of “liver being the congenital dependence of women” in the etiology， pathogenesis， syndrome differentiation and treatment of women’s diseases， from the role of liver in women’s physiological processes and pathological changes based on the theories of liver storing blood and liver governing conveyance and dispersion.关键词：liver stores blood;liver governs conveyance and dispersion;Ye Tianshi;liver is the congenital dependence of women
- 15
- |
- 799
- |
- 0
发布时间：2022-08-12 - 摘要：The prevalence of chronic kidney disease （CKD） is increasing year by year and has become one of the major public health issues of global concern in recent years. Depression is the most common psychological disorder in CKD patients， which seriously affects their life and treatment. Timely attention to the state of depression in CKD patients and early intervention are more beneficial to the treatment and prognosis of these psychosomatic diseases. Our research group has proposed to treat CKD with depression by Xiaochaihu Decoction together with acupuncture， which is a new treatment logic developed from the concept of “hybrid therapies” in Huangdi Neijing （The Yellow Emperor’s Inner Canon）， supported by the experience of combining acupuncture with classic formulas to treat psychosomatic diseases in Shang Han Lun （Treaties on Febrile Diseases） and the analysis of related clinical and fundamental research progress.关键词：chronic kidney disease;depression;Xiaochaihu Decoction;acupuncture;hybrid therapies;psychosomatic disease
- 18
- |
- 273
- |
- 0
发布时间：2022-08-12 - 摘要：ObjectiveTo evaluate the efficacy and safety of Yiqi Yangyin Jiedu （YQYYJD） regimen of traditional Chinese medicine（TCM） plus anlotinib for advanced non-small cell lung cancer （NSCLC）.MethodsA retrospective study approach was applied to collect information on advanced NSCLC cases treated with the YQYYJD regimen of TCM plus anlotinib from June 2018 to March 2021 through the electronic medical record system. All cases were followed up until November 1， 2021， and general information such as driver gene mutation， previous treatment， and pathological types were recorded to evaluate the outcome of solid tumors， and changes in Eastern Cooperative Oncology Group （ECOG） score and the incidence of adverse events were analyzed. Median progression free survival （PFS） and median overall survival （OS） were calculated by the Kaplan-Meier method. Cox analysis was used to evaluate the significance of factors influencing PFS and OS.Results①A total of 33 cases of advanced NSCLC treated with YQYYJD regimen of TCM plus anlotinib were included in the study， and 12 cases harbored driver gene mutation. All patients had previously received epithelial growth factor receptor tyrosine kinase inhibitor （EGFR-TKI）， anaplastic lymphoma kinase tyrosine kinase inhibitors （ALK-TKI）， or first- and second-line chemotherapy， and acquired resistance， and 20 patients progressed after at least two lines of chemotherapy. The median line of therapy for anlotinib was three. ②The median progression free survival （PFS） was 7.0 months （95% CI： 2.7-11.3）， and the median overall survival （mOS） was 16.0 months （95% CI： 12.4-19.6） in 33 cases. ③In evaluating best outcomes of solid tumors， there were 3 cases of partial response， 29 cases of stable disease， and 1 case of disease progression， with an objective response rate of 9.1% and a disease control rate of 97.0%. ④During the treatment， only 1 case of grade 3 treatment-related adverse effects （renal impairment） and reduced dose of anlotinib was reported. ⑤During the course of treatment 22 cases （66.7%） reported stable performance status. ⑥Cox multifactorial analysis demonstrated that elevated TSH level after treatment was significantly associated with longer PFS （RR=3.971， 95% CI： 1.524 - 10.347， P=0.005）， and no independent prognostic factors related to OS was found； The mPFS of patients with elevated TSH after treatment was 12 months， longer than that of patients without elevated TSH （ mPFS was 5 months） （RR=0.197， 95% CI： 0.067 - 0.574， P=0.002 9）.ConclusionYQYYJD regimen of TCM may prolong survival of advanced NSCLC patients by reducing adverse effects of anlotinib and stabilizing general performance status.关键词：non-small cell lung cancer;anlotinib;traditional Chinese medicine therapy;clinical efficacy;survival;efficacy enhancing and toxicity reducing;retrospective study
- 20
- |
- 306
- |
- 0
发布时间：2022-08-12 - 摘要：ObjectiveTo observe the clinical effect of fistulotomy combined with marsupialization on the treatment of low anal fistula.MethodsEighty patients with low anal fistula were randomly divided into a treatment group （n=40） and a control group （n=40）. The control group was treated with fistulotomy alone， and the treatment group was treated with fistulotomy and marsupialization. The clinical effect was observed， the duration of surgery， the area of surgical wound， the visual analogue scale （VAS） score of postoperative pain， the amount of postoperative wound exudation， the time taken for wound healing， the scar area at healing， and the Wexner score of postoperative anal incontinence were compared between the two groups.Results①After treatment， patients in both groups were cured clinically， and there was no statistically significant difference in the clinical effect between the two groups （P>0.05）. ②The treatment group took longer time to operate than the control group， the average pain VAS score of the treatment group was lower than that of the control group， the average amount of wound exudation of the treatment group was less than that of the control group， and these differences were statistically significant （P<0.05）. ③The surgical wound area and scar area at healing in the treatment group were smaller than those in the control group， the time taken for wound healing was shorter than that in the control group， and these differences were statistically significant （P<0.05）. ④The difference in the Wexner score of postoperative anal incontinence between the two groups was not statistically significant （P>0.05）.ConclusionFistulotomy combined with marsupialization can effectively and safely treat low anal fistulas with a shorter treatment course and milder postoperative complications than fistulotomy alone， without increasing the risk of anal incontinence.关键词：low anal fistula;anal fistulotomy;marsupialization;pain;wound healing;clinical trial
- 13
- |
- 107
- |
- 0
发布时间：2022-08-12 - 摘要：ObjectiveTo investigate the clinical effect of Jiawei Mojie Tablets on primary dysmenorrhea of qi stagnation and blood stasis syndrome.MethodsSeventy-three cases of primary dysmenorrhea of qi stagnation and blood stasis syndrome were randomly divided into a treatment group （n=35） and a control group （n=38）. The control group was administered with ibuprofen， while the treatment group was administered with Jiawei Mojie Tablets. The duration of treatment for both groups was 3 menstrual cycles. We observed the clinical efficacy， and compared the changes of pain visual analogue scale （VAS） scores， traditional Chinese medicine （TCM） symptom scores， the serum levels of inflammatory factors and β-endorphin， the Zung Self-Rating Anxiety Scale （SAS） scores and the Zung Self-Rating Depression Scale （SDS） scores before and after intervention.Results①During the trial， there were 3 drop-out cases in the treatment group and 4 drop-out cases in the control group， and 32 cases in the treatment group and 34 cases in the control group finally completed the trial. ②The total effective rate was 78.12% in the treatment group and 55.88% in the control group. The clinical efficacy of the treatment group was better than that of the control group （P<0.05）. ③After treatment， the pain VAS scores decreased in both groups （P<0.05）； The pain VAS scores in the treatment group were lower than those in the control group （P<0.05）. ④After treatment， the sub-dimension scores of abdominal pain， menstrual blood clots， breast distension and pain， anal pendant expansion and total TCM symptom scores decreased in the treatment group （P<0.05）， and the sub-dimension scores of abdominal pain and total TCM symptom scores decreased in the control group （P<0.05）； After treatment， the sub-dimension scores of abdominal pain， menstrual blood clots， breast distension and pain， anal pendant expansion and total TCM symptom scores in the treatment group were lower than those in the control group （P<0.05）. ⑤After treatment， the serum level of interleukin-6 （IL-6） decreased （P<0.05） and the serum levels of interleukin-10 （IL-10） and β-endorphin increased （P<0.05） in the treatment group，the serum levels of β-endorphin increased （P<0.05） in the control group. After treatment， the differences in serum levels of IL-6， IL-10 and β-endorphin between the two groups were not statistically significant （P>0.05）. ⑥After treatment， SDS scores decreased in the treatment group （P<0.05）， but SAS and SDS scores did not change significantly in the control group （P>0.05）. After treatment， SDS scores in the treatment group were lower than those in the control group （P<0.05）.ConclusionsJiawei Mojie Tablets can effectively alleviate the clinical symptoms and improve the depressed emotional state of patients with primary dysmenorrhea of qi stagnation and blood stasis syndrome. The mechanisms involved may be that Jiawei Mojie Tablets can lower IL-6 level， raise IL-10 level， reduce inflammation， and promote the secretion of endogenous opioid analgesic substance β-endorphin， which in turn lowers pain threshold.关键词：primary dysmenorrhea;qi stagnation and blood stasis syndrome;TCM Master;traditional Chinese medicine therapy;inflammatory factor;clinical trial
- 15
- |
- 281
- |
- 0
发布时间：2022-08-12 - 摘要：ObjectiveTo observe the clinical efficacy of Buxu Qufeng Decoction in the treatment of Ⅳstage diabetic kidney disease （DKD）.MethodsA total of 85 patients with Ⅳstage DKD were randomly divided into the treatment group（43 cases） and the control group（42 cases）. Both groups were given basic treatment， the control group was given losartan ， while the treatment group was given Buxu Qufeng Decoction on the basic treatment of the control group. The course of treatment in both groups was 6 months. After the treatment， the clinical efficacy were observed， and the changes in TCM syndrome score， blood glucose， urinary protein， and renal function related indexes were compared.Results①During the experiment， 3 cases in the treatment group and 2 cases in the control group were shed， and 80 cases were finally completed. ②After the treatment， the total effective rate in the treatment group was 90.0% ， and the control group was 47.5% ； the clinical effect of the treatment group was significantly better than that of the control group（P<0.05）. ③After the treatment， the scores of TCM syndrome were decreased in both groups， and the difference of TCM syndrome scores in the treatment group was significantly better than that in the control group（P<0.05）；④After the treatment， FPG， 2 hPG and HbA1c in two groups were decreased （P<0.05）， but there were no significant differences in FPG， 2hPG and HbA1c between groups（P>0.05）. ⑤After treatment， ACR and 24 hUTP in the treatment group were decreased （P<0.05）， serum albumin was increased （P<0.05）， but there was no significant change in the control group （P>0.05）. ACR and 24 hUTP in the treatment group were significantly lower than those in the control group （P<0.05）. ⑥After treatment， serum creatinine， blood urea nitrogen and CYC in the treatment group were decreased （P<0.05）， while eGFR was increased （P<0.05）. In the control group， serum creatinine， blood urea nitrogen and CYC were higher than before treatment （P<0.05）， while eGFR was lower （P<0.05）. After treatment， serum creatinine， blood urea nitrogen， CYC and eGFR in the treatment group were better than those in the control group （P<0.05）.ConclusionBuxu Qufeng Decoction combined with routine therapy could improve the clinical symptoms of Ⅳstage DKD patients， reduce urinary protein， and protect kidney function.关键词：diabetic kidney disease;chronic kidney disease;Buxu Qufeng Decoction;traditional Chinese medicine therapy;clinical trial
- 9
- |
- 132
- |
- 0
发布时间：2022-08-12 - 摘要：ObjectiveThe molecular mechanism of timosaponin AⅢinhibiting the proliferation on murine glioblastoma GL261（GL261 cell） was investiated by proteomics.Methods① GL261 cells were divided into blank control group （timosaponin AⅢ 0 μmol/L） and different dose groups of timosaponin AⅢ （1， 2， 4， 6， 8， 10 μmol/L）. And solvent control group ［0.05% dimethyl sulfoxide （DMSO）］ and positive drug group ［taxol（TAX），1 μmol/L］ were set up. According to the grouping， the solution was added to DMEM containing 10% fetal bovine serum （FBS）， and then mixed into 96-well plate. After incubation for 72 h， the cell survival rate of each group was detected by MTT method. ② GL261 cells were divided into blank control group and timosaponin AⅢ group （3.5， 7 μ mol/L）. According to the grouping， the solution was added to DMEM containing 10%FBS， mixed and then added to 96-well plate for 24 h. The colony formation of the cells was determined by plate cloning， apoptosis detected by flow cytometry， and the potential target proteins and related signal pathways analyzed by proteomics. Western blot was used to verify the key proteins.ResultsCompared with the blank control group， timosaponin AⅢ significantly inhibited the viability and colony formation of GL261 cells and induced apoptosis of GL261 cells in a dose-dependent manner. Proteomic results showed that there were 16 differential proteins in GL261 cells treated with timosaponin AⅢ， of which 5 proteins were up-regulated and 11 proteins were down-regulated. Gene ontology （GO） analysis showed that the main biological processes， cell composition and molecular functions focused on proliferation regulation， negative growth regulation， organelles， protein binding， protein homodimer activity and so on. Kyoto Encyclopedia of Gene and Genome （KEGG） analysis showed that the main pathways were concentrated in mucin O-glycan biosynthesis， non-small cell lung cancer， bacterial invasion of epidermal cells， osteoclast differentiation， FoxO signal pathway， Wnt signal pathway， tight junction， cell adhesion molecules， proteoglycan in cancer and Ras signal pathway. The results of Western blot showed that compared with the blank control group， timosaponin AⅢ inhibited the expressions of CD276 protein and the key proteins in Wnt signaling pathway， such as β-catenin， proto-oncogene （c-Myc） and cyclin D1 （CyclinD1） in GL261 cells in a dose-dependent manner（P<0.05）.ConclusionsTimosaponin AⅢ can inhibit the proliferation and colony formation of GL261 cells in vitro and induce apoptosis of GL261 cells. The mechanism may be that it inhibits the expression of CD276 protein and regulates the Wnt signal pathway.关键词：glioblastoma;timosaponin AⅢ;proteomics;CD276;Wnt signal pathway;research of traditional Chinese herbal medicine
- 15
- |
- 352
- |
- 0
发布时间：2022-08-12 - 摘要：ObjectiveTo observe the proliferation of lung adenocarcinoma H1975 cells resistant to gefitinib in vitro under the action of Feiyanning Prescription （FYN） and gefitinib， and to explore the possible mechanism of FYN in reversing gefitinib resistance.MethodsLung adenocarcinoma T790M mutation-resistant strain H1975 cells were selected and divided into control group， gefitinib group， FYN group and combination group. CCK-8 assay was used to detect the proliferation rate of cells in each group at different time （0 h， 24 h， 48 h， and 72 h）； Western blot was used to detect the expression of proteins related to EGFR-PI3K/Akt/mTOR signal pathway.ResultsThe results of CCK-8 showed that FYN inhibited the proliferation of H1975 cells in vitro in a dose-dependent manner， that is， its inhibitory power increased with the increase of drug concentration， and the effect of combination drug on inhibiting cell proliferation in vitro was more obvious than that of single drug. Western blot showed that FYN combined with gefitinib could down-regulate the expression of p-EGFR， p-Akt and p-mTOR.ConclusionFYN combined with gefitinib can inhibit the proliferation of H1975 cells in vitro， and the inhibitory effect in vitro is more significant than that of gefitinib. The mechanism of delaying gefitinib resistance may be related to the down-regulation of EGFR-PI3K/Akt/mTOR signal pathway related protein expression.关键词：lung adenocarcinoma;Feiyanning Prescription;gefitinib;H1975 cells;proliferation in vitro;traditional Chinese herbal medicine research
- 10
- |
- 192
- |
- 0
发布时间：2022-08-12 - 摘要：ObjectiveTo observe the effects of Tiaoxin Bushen Formula （TXBSF） on the activation of microglia and neuroinflammatory response in prefrontal cortex （PFC） of presenilin 1/2 conditional double knockout （PS cDKO） mice with Alzheimer’s disease.MethodsTotally 60 PS cDKO mice aged 3-3.5 months and their littermates were randomly divided into three groups with 20 in each： wild type group （WT）， model group （PS cDKO） and model+TXBSF group （PS cDKO+TXBSF）. The mice in the WT and PS cDKO group were fed with standard chow， and the mice in the PS cDKO+TXBSF group were fed with standard chow containing TXBSF （17.918 g/kg） for 90 days. After drug administration， the mice were sacrificed for molecular biology detection. Immunohistochemical staining was used to observe the effect of TXBSF on the activation of microglia in PFC of mice in each group. qRT-PCR was used to analyze the effect of TXBSF on mRNA levels of proinflammatory factors in PFC of mice， such as cyclooxygenase-2 （COX-2）， inducible nitric oxide synthase （iNOS）， tumor necrosis factor-α （TNF-α）， interleukin-1β （IL-1β） and interleukin-6 （IL-6）. Western blot was used to detect the effect of TXBSF on the expressions of COX-2 protein and iNOS protein in PFC of mice in each group， and changes in the levels of TNF-α， IL-6 and IL-1β in PFC of mice in each group were measured by ELISA.ResultsImmunohistochemical staining showed that compared with PS cDKO mice， the mice with TXBSF demonstrated lower ratios of amoeboid microglia（P<0.05）， more branching numbers （P<0.05）， longer average branch length （P<0.05） and longer maximum branch length（P<0.05）. The result of qRT-PCR showed that TXBSF significantly inhibited COX-2， iNOS， TNF-α，IL-1β and IL-6 mRNA expression levels（P<0.05）. Western blot analysis showed that the protein expression levels of COX-2 and iNOS in PFC of PS cDKO mice treated with TXBSF were significantly lower than those in PS cDKO group （P<0.05）. The result of ELISA showed that compared with those of PS cDKO mice， the treatment of TXBSF could significantly reduce the levels of TNF-α，IL-6 and IL-1β in PFC of PS cDKO mice （P<0.05）.ConclusionTXBSF may improve the memory impairment by inhibiting the activation of microglia and neuroinflammatory responses in PFC of mice with Alzheimer’s disease.关键词：Alzheimer’s disease;TXBSF;microglia;neural inflammation;PS cDKO;mouse model;traditional Chinese herbal medicine research
- 16
- |
- 389
- |
- 0
发布时间：2022-08-12 - 摘要：ObjectiveTo establish an ultra high performance liquid chromatography （UPLC） fingerprint of Rabdosiae Rubescentis Herba formula granules and determine the multi-component contents as a quality control method.MethodsWaters ACQUITY UPLC HSS T3 column （2.1 mm × 100 mm，1.8 μ m） was used with acetonitrile and 0.1% formic acid aqueous solution as mobile phase for gradient elution with a flow rate of 0.4 mL ·min-1. The detection wavelength was 350 nm， the column temperature was 30 ℃， and the injection volume was 3 μL.ResultsThe fingerprints of 10 batches of Rabdosiae Rubescentis Herba formula granules were established， 8 common peaks were obtained， and 4 component peaks were identified， namely caffeic acid， ferulic acid， rosmarinic acid and pedalitin， with a similarity of 0.999～1.000. The four components showed a good linear relationship in their respective concentration range （R2=0.999 7～1.000 0）. The average recoveries were between 90.27% and 110.64% with RSD of 0.07% ～2.16%.ConclusionThis method has good repeatability， stability and feasibility， meets the analysis requirements of fingerprint， and can determine four components at the same time， which provides a reference basis for establishing the quality control standard of Rabdosiae Rubescentis Herba formula granules.关键词：Rabdosiae Rubescentis Herba formula granules;UPLC;fingerprint;content determination;traditional Chinese herbal medicine research
- 20
- |
- 466
- |
- 0
发布时间：2022-08-12 - 摘要：ObjectiveTo establish gas chromatography-mass spectrometry （GC-MS） for the comparative analysis of the content and components of essential oil from Amomi Fructus in different regions in order to study the original features of Amomi Fructus.MethodsBy compared the essential oil content of the seed masses in Amomi Fructus samples from the main producing areas of Guangdong， Yunnan and Guangxi provinces， the essential oil of Amomi Fructus from different regions was separated and identified by GC-MS on a DB-1ms column （30 m×0.25 mm， 0.25 μm）， and the relative contents of each compound were determined by area normalization method. Correlation analysis and cluster analysis were used to analyze and evaluate the components of Amomi Fructus samples from 20 batches of different producing areas.ResultsThe method had high precision， repeatability and stability， and met the qualitative and quantitative requirements. The results showed that there was a certain degree of difference in the essential oil content of Amomi Fructus samples from different regions. Twelve common chemical components were identified from 20 batches of essential oil of Amomi Fructus from different origins， of which the main components were bornyl acetate， camphor， limonene and borneol. There were obvious differences among some components from different origins. Correlation analysis showed that α-copaene and （+）-aromandendrene， β-myrcene and D-limonene were positively correlated （correlation coefficient： 0.968， 0.938， P<0.01）， respectively. The samples of Amomi Fructus from Guangdong， Yunnan and Guangxi could be accurately classified according to the results of cluster analysis respectively.ConclusionsAmomi Fructus has obvious origin clustering characteristics， and there are certain differences in chemical composition of essential oil of Amomi Fructus from different origins. Different production areas of Amomi Fructus can be distinguished by detecting specific components. The established method and experimental results can provide theoretical basis for the development and quality evaluation of Amomi Fructus resources.关键词：Amomi Fructus;essential oil;GC-MS;correlation analysis;cluster analysis;traditional Chinese herbal medicine research
- 15
- |
- 561
- |
- 0
发布时间：2022-08-12 - 摘要：ObjectiveTo observe the intervention effects of medicated serum of Liuwei Dihuang Decoction on Runt-related transcription factor 2 （Runx2） and osteoprotegerin / nuclear factor-κB receptor activator ligand （OPG/RANKL） in preparietal osteoblasts （MC3T3-E1） of mice with oxidative injury induced by hydrogen peroxide （H2O2）， and to explore its mechanism from the point of view of antioxidation.MethodsThe Liuwei Dihuang Decoction was prepared and given to the adult Wistar rats by intragastric administration，and then the medicated serum of Liuwei Dihuang Decoction was collected. Except for the normal group， the other groups of MC3T3-E1 cells were first pretreated with 1.0 mmol/L H2O2 for 6 h， and then the culture medium of the model group was replaced with normal culture medium， the N-acetylcysteine （NAC） group replaced with 2.5 mmol/L NAC and the medicated serum groups with 10% medicated serum. Each group was treated for 24 hours. The level of reactive oxygen species （ROS） in MC3T3-E1 cells was detected by DCFH-DA fluorescence probe. Catalase （CAT）， superoxide dismutase （SOD）， glutathione peroxidase （GSH-PX） and reduced glutathione （GSH） were detected according to the kit instructions. The expressions of Runx2， OPG， RANKL， tumor suppressor protein p53 and phosphorylated p53 protein （p-P53） were detected by Western blot.ResultsCompared with the normal group， ROS in the model group was significantly increased （P<0.05）， while CAT， SOD， GSH-PX， and GSH were significantly decreased （all P<0.05）. In terms of protein expression， compared with the normal group， the protein expressions of Runx2 and OPG in the model group were significantly decreased （all P<0.05）， while the RANKL was significantly increased （P<0.05）， and p-P53/P53 was also significantly increased （P<0.05）. Compared with the model group， the GSH in the medicated serum group of Liuwei Dihuang Decoction was significantly increased （P<0.05） and the level of ROS was significantly decreased （P<0.05）. In terms of protein expression， compared with the model group， the protein expressions of Runx2 and OPG in the medicated serum group of Liuwei Dihuang Decoction were significantly increased （P<0.05）， while the expression of RANKL was significantly decreased （P<0.05） ， and p-P53/P53 was also significantly decreased （P<0.05）.ConclusionsIn H2O2-induced oxidatively damaged MC3T3-E1 cells， the medicated serum of Liuwei Dihuang Decoction could promote the protein expression of Runx2， and it could increase the protein expression of OPG and decrease the protein expression of RANKL. This may be related to the fact that it could increase the content of GSH， inhibit the phosphorylation of P53 protein， and reduce the level of ROS in MC3T3-E1 cells.关键词：osteoporosis;Liuwei Dihuang Decoction;Runx2;OPG/RANKL;osteoblast cells;traditional Chinese herbal medicine research
- 11
- |
- 325
- |
- 0
发布时间：2022-08-12 - 摘要：ObjectiveTo observe the effect of Polygoni Multiflori Caulis （PMC） and PMC plus Astragali Radix （AR） on concanavalin A （ConA） induced immunological liver injury （ILI） in model mice.MethodsSeventy healthy ICR mice （SPF grade） were randomly divided into normal group， model group， normal group plus high-dose or low-dose PMC， model group plus high-dose or low-dose PMC， model group plus high-dose PMC and AR， with 10 mice in each group. After 10 consecutive days of intragastric administration， ConA with 10 mg·kg-1 injected intravenously was used to induce the ILI model at the last intragastric administration in all groups except the normal groups. 8 hours later， the mice were sacrificed and samples were collected. The pathological changes of liver were observed by HE staining. Liver function （ALT， AST， MDA， MAO， GST， DBIL， IBIL） and blood lipid （TC， TG， LDL-C） were detected by biochemical method.ResultsCompared with the normal group， liver weight， liver index， ALT， AST， MDA and MAO of the model group were increased （P<0.05）， while TG and GST were decreased （P<0.05）. Liver HE staining showed a large number of infiltrated inflammatory cells in the model group. After PMC given （high or low doses）， ALT， AST， MDA and MAO levels were increased significantly （P<0.05）， while GST was decreased （P<0.05）. Additionally， HE staining showed more inflammatory cell infiltration with partial necrosis of liver cells in the model group plus high-dose PMC. However， ILI mice administrated with combination of high-dose PMC and AR， the liver weight， liver index， ALT， AST， MDA and MAO were decreased （P<0.05）， but GST was increased （P<0.05）. Liver HE staining also showed less inflammatory cell infiltration and necrocytosis.ConclusionPMC can further destroy the liver function of ILI mice， while RA can produce certain protective effect.关键词：drug-induced liver injury;immunological liver injury;Polygoni Multiflori Caulis;Astragali Radix;mouse model;traditional Chinese herbal medicine research
- 13
- |
- 410
- |
- 0
发布时间：2022-08-12 - 摘要：To deal with the nonstandard， inconsistent and unimportant clinical research evaluation indicators， methodologists put forward the concept of core outcome set （COS）. The establishment of core outcome measures in effectiveness trials （COMET） working group has facilitated the international promotion and application of COS. This paper expounds the concept and function of COS， COS research platforms， COS development methods， and the core outcome set of traditional Chinese medicine （COS-TCM） for clinical trials， thereby providing references for the research of core outcome set.关键词：core outcome set;evaluation indicator;methodology;traditional Chinese medicine;evidence-based medicine;clinical trial
- 7
- |
- 532
- |
- 0
发布时间：2022-08-12
- Technical support is provided by Beijing Founder electronics co., LTD 沪ICP备13044091号
沪公网安备 31011502015228号 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
- Total Visits：70575 Visits Today：56
0