最新刊期
期 7 ， 2022
- 摘要：Omicron variant of SARS-CoV-2 has the characteristics of rapid transmission， insidiousness， and relatively decreased virulence. Most infected patients have relatively mild clinical symptoms， but a certain proportion of elderly people with underlying diseases still turn into severe or critical cases. Since the spring of 2022， community and cluster transmission of COVID-19 have coexisted in Shanghai， and a high proportion of severe cases are those elderly with underlying diseases. Therefore， the key to reducing the mortality rate of COVID-19 lies in the effective treatment of severe cases. To treat and cure severe cases， Shanghai has established a “multidisciplinary joint diagnosis and treatment” model， insisting on the integrated application of traditional Chinese and western medicine， and emphasizing early intervention， syndrome differentiation-based treatment， one-person-one-policy， one-person-one-prescription， and preemptive treatment for disease interception， for the purpose of effective disease progression control and decrease in mortality rate. The article summarizes Shanghai’s diagnosis and treatment model and experience in treating severe COVID-19 patients and tries to improve the technical system of traditional Chinese medicine in treating severe COVID-19 cases.关键词：SARS-CoV-2;Omicron variant;COVID-19;severe case;Shanghai;traditional Chinese medicine therapy;integrated traditional Chinese and western medicine
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发布时间：2022-10-09 - 关键词：SARS-CoV-2;COVID-19;Omicron variant;traditional Chinese medicine therapy;expert consensus
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发布时间：2022-10-09 - 摘要：ObjectiveTo investigate Shanghai residents’ acceptance of traditional Chinese medicine （TCM） for combating novel coronavirus disease （“COVID-19”） pandemic.MethodsConvenience and volunteer sampling methods were used to investigate the acceptance of TCM to combat the COVID-19 among medical personnel， community volunteers and residents in Shanghai.Results①A total of 662 valid questionnaires were collected. Among the 662 respondents， 41.1% were male， the age ranged from 20 to 60 years， and 29.2% were engaged in the medical profession. The results of univariate analysis showed that gender， age， occupation， education level， and personal monthly income had no significant effect on the acceptance of TCM. ②In this survey， 639 respondents believed in TCM treatment， accounting for 96.5% of the total. Among them， 584 respondents had used TCM before， accounting for 91.4%； 600 respondents showed their willingness to recommend others to use TCM for the pandemic prevention and control， accounting for 93.9%； and 500 respondents recommended TCM prescriptions alone to fight against the COVID-19， accounting for 78.2%. ③Compared with respondents who had never used TCM， those who had used TCM before were more likely to recommend others to use TCM for the pandemic prevention and control （P=0.004）； those who had used TCM before were more concerned about the flavor and other issues of TCM （P=0.054， P=0.003）， and those who had not used TCM before were more concerned about effectiveness and safety of TCM （P=0.005，P=0.045）. ④Residents in Shanghai showed a wide range of demands for TCM， and the strongest ones were ranked in descending order： TCM treatment， TCM prevention and TCM science popularization， with a frequency of 452， 443， and 424， respectively.ConclusionsShanghai residents have a high level of acceptance of TCM and its use in the prevention and treatment of COVID-19 pandemic. Therefore， it is recommended to further the application of TCM in the pandemic prevention and control in response to the realistic demands.关键词：coronavirus disease （COVID-19）;SARS-CoV-2;Omicron variants;pandemic prevention and control;traditional Chinese medicine therapy;sampling survey
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发布时间：2022-10-09 -
Application of insect drugs in treatment of post⁃infectious cough based on collateral disease theory
摘要：The pathogenesis of collateral disease is characterized by stasis and stagnation. Insect drugs are good at dispelling the wind and dredging collaterals， resolving phlegm and removing stasis.Post-infectious cough and collateral disease share the similar pathogenesis. Therefore， in this article， we discussed the application of insect drugs in the clinical treatment of post-infectious cough based on the theory of collateral disease for the purpose of providing new insights for the treatment of post-infectious cough.关键词：post-infectious cough;collateral disease;etiology and pathogenesis;insect drug;traditional Chinese medicine therapy;traditional Chinese herbal medicine- 15
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发布时间：2022-10-09 - 摘要：Cervical spondylotic myelopathy （CSM） is the most serious subtype of cervical spondylosis， characterized by severe clinical symptoms， a high disability rate， and poor prognosis. In 2021， the National Technical Committee for Standardization of Integrative Traditional Chinese and Western Medicine promulgated the Expert Consensus on Diagnosis and Treatment of Cervical Spondylotic Myelopathy with Integrative Traditional Chinese and Western Medicine， which provided a comprehensive introduction to the definition， etiology， pathogenesis， diagnosis， treatment principles， integrated traditional Chinese and western medicine treatment methods， postoperative rehabilitation， nursing and aftercare of CSM. The consensus has been interpreted comprehensively to achieve a better understanding among clinicians.关键词：cervical spondylotic myelopathy;integrated traditional Chinese and western medicine;clinical diagnosis and treatment;expert consensus;interpretation
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发布时间：2022-10-09 - 摘要：Prolapsed/sequestrated lumbar disc herniation has a tendency to resorb， and traditional Chinese medicine （TCM） conservative treatment can facilitate the process of resorption. The disease can often be relieved or even cured by non-surgical treatment with traditional Chinese medicine. This paper discusses the incidence of disc resorption in these two types of lumbar disc herniation， methods for predicting the occurrence of resorption， selection of non-surgical TCM treatment， and assessment of treatment risks.关键词：lumbar disc herniation;conservative treatment;traditional Chinese medicine therapy;disc resorption;risk assessment
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发布时间：2022-10-09 - 摘要：ObjectiveTo systematically evaluate the therapeutic effects of balance acupuncture on scapulohumeral periarthritis.MethodsThe literature of clinical randomized controlled trials on balanced acupuncture for the treatment of scapulohumeral periarthritis was searched in the databases of Chinese National Knowledge Infrastructure （CNKI）， Wan Fang Data， VIP （Chongqing VIP Chinese Scientific Journals Database）， China Biology Medicine disc （CBM disc）， PubMed and Web of Science from the date of database establishment up to June 28， 2021， and the included literature was assessed for risk of bias using RevMan 5.3， and meta-analysis was performed with Stata 16.0.ResultsOverall， 28 pieces of eligible literature and 13，354 patients （experimental group： 11，447； control group： 1，907） were included in this study. According to the results of meta-analysis， we observed that the treatment group with balanced acupuncture had better clinical outcomes ［RR=0.95， 95% CI： 0.91~0.98， P=0.004］ and better improvement in visual analogue scale （VAS） score of pain ［WMD=-1.90， 95% CI： -2.71~-1.09， P<0.001］， shoulder activity scale scores ［elevation： WMD=11.20， 95% CI： 7.10~15.29， P<0.001； abduction： WMD=7.40， 95% CI： 4.29 - 10.52， P<0.001 ； posterior extension： WMD=4.42， 95% CI： 2.36~6.49， P<0.001； external rotation： WMD=8.62， 95% CI： 7.65~9.59， P<0.001， and CMS score ［WMD=7.50， 95% CI： 5.26~9.74， P<0.001］ than the control group.ConclusionsBalance acupuncture is effective in treating scapulohumeral periarthritis， which can effectively alleviate pain and improve limited shoulder joint movement. However， the conclusions obtained have to be validated by future studies due to the limited quantity and quality of the literature.关键词：scapulohumeral periarthritis;acupuncture;balance acupuncture;clinical therapeutic effect;Meta-analysis
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发布时间：2022-10-09 - 摘要：Professor Liu Ximing’s experience in treating tinnitus from fire and heat was introduced. According to “tinnitus was caused by the rise of yang qi， just like the growth of all things” in Interpretation of Pulse， Su Wen， the theory that fire burning was the core pathogenesis of tinnitus was proposed. Guided by the theory of deficiency and excess， the pathogenesis of fire and heat could be divided into yang excess， qi stagnation， pathogen obstruction and yin deficiency. Correspondingly， treatments could be adopted in four ways of clearing heat and fire， promoting qi and relieving depression， dispelling pathogen， and replenishing yin and yang. Messenger drugs such as Scallion， Acori Tatarinowii Rhizoma and Magnetitum were essential for efficacy. By regulating the balance of zang-fu， adjusting deficiency and excess， and clearing the origins of fire and heat， hearing would be recovered. Four cases were appended to illustrate the theory.关键词：tinnitus;fiery syndrome;traditional Chinese medicine therapy;Liu Ximing
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发布时间：2022-10-09 - 摘要：The clinical experience of Professor Ding Yuanqing in the treatment of medication-overuse headache is introduced by analyzing a proven medical case. It is considered that the syndrome is caused by long-term headache， improper use of medication. In this article， the pathogenesis blood deficiency and collaterals obstruction， which is closely related to Jueyin liver and pericardium， is proposed firstly. The main pathogenesis is deficiency of yin and blood and the secondary one is endogenous wind disturbing collaterals and turbid phlegm causing blood stasis. Long-term disease will enter the collaterals， resulting in disharmony of body and spirit. In clinic， based on the theory of syndrome differentiation of the six meridians for headache， the syndrome can be treated by the methods of supplementing deficiency and purging excess-evil， simultaneously treating body and mind. Therefore， a good result can be received in treating the syndrome by using the modified prescription according to the change of syndrome as the Sishen Powder has the functions of expelling wind and dredging collaterals， regulating qi and blood， and dispersing obstruction to stop pain. One case was appended to illustrated the therapy.关键词：medication-overuse headache;Jueyin headache;Sishen Powder;blood deficiency and collaterals obstruction;simultaneously treating body and mind;traditional Chinese medicine therapy
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发布时间：2022-10-09 - 关键词：neuromyelitis optica spectrum disorder;painful spasm;Xiewu Capsule;convulsiue disease;spasm;yin-yang poisoning;proven case
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发布时间：2022-10-09 - 摘要：A model of six meridians system containing three yin meridians and three yang meridians is constructed based on Huangdi Neijing （The Yellow Emperor’s Inner Canon）. The model contains the traditional Chinese medicine （TCM） concepts of yin and yang， exterior and interior， internal organs， meridians and collaterals， three yin and three yang， twelve divisions and sequence， and these concepts can be mutually corroborated and interconnected in this system. The model’s explanation has defused controversies over the nature of the six meridians in history， reflects the holistic view of the six meridians system with three yin and three yang， and corresponds with “theory of synthesized concepts”.关键词：Huangdi Neijing;Shanghan Lun;three yin and three yang;model diagram of six meridians system
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发布时间：2022-10-09 - 摘要：ObjectiveTo investigate the effect of Modified Gegen Qinlian Decoction （MGQD） on inflammatory factors and immune cells in patients with colorectal cancer.MethodsA total of 82 patients with colorectal cancer to be treated by operation were randomly divided into a control group （n=41） and a treatment group （n=41）. Both groups were administered with conventional treatment and confine operation， and the treatment group was additionally administered with MGQD 7 days before operation. The changes of immune cells ［CD4+T cells， CD8+T cells， natural killer （NK） cells， natural killer T （NKT） cells， and Treg cells］ and inflammatory factors ［tumor necrosis factor-α （TNF-α）， interferon-gamma （IFN-γ）， interleukin-2 （IL-2）， interleukin-6 （IL-6）， interleukin-10 （IL-10）， and 5-hydroxytryptamine （5-HT）］ levels in plasma， as well as the changes of 5-HT and TNF-α expression in tumor tissues were compared between the two groups.Results①After treatment， the plasma levels of CD4+ T cells and NKT cells in the treatment group increased （P<0.05）， but there was no significant difference in the plasma levels of CD4+ T cells， NKT cells， CD8+ T cells， NK cells and Treg cells before and after treatment in the control group （P>0.05）； After treatment， the levels of CD4+ T cells and NKT cells in the treatment group were higher than those in the control group （P<0.05）. ②According to the intra-group comparison before and after treatment， the plasma levels of TNF-α， IFN-γ， IL-2， IL-6， IL-10， and 5-HT decreased in the treatment group （P<0.05）， but the differences in the levels of the above inflammatory factors in the control group were not statistically significant （P>0.05）； According to the inter-group comparison after treatment， the plasma levels of TNF-α， IFN-γ， IL-2， IL-6， IL-10， and 5-HT in the treatment group were lower than those in the control group （P<0.05）. ③The intra-group comparison before and after treatment showed that the IOD values of TNF-α and 5-HT in the tumor tissues of the treatment group decreased （P<0.05）， but there was no significant difference in IOD values of TNF-α and 5-HT in the tumor tissues of the control group （P>0.05）； The inter-group comparison after treatment showed that the IOD values of TNF-α and 5-HT in the tumor tissues of the treatment group were lower than those of the control group （P<0.05）.ConclusionThe application of MGQD is promising in the clinic as it can effectively regulate the immune level and inflammatory response in colorectal cancer patients.关键词：colorectal cancer;Gegen Qinlian Decoction;immunoregulation;inflammatory factor;traditional Chinese medicine therapy;clinical trial
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发布时间：2022-10-09 - 摘要：ObjectiveTo observe the clinical efficacy of modified Xuanbai Chengqi Decoction （XBCQ） in the treatment of mycoplasma pneumoniae pneumonia （MPP） of phlegm-heat blocking lung syndrome， investigate the effect of modified XBCQ on the intestinal microflora and the content of secretory immunoglobulin A （sIgA） in pediatric patients， and explore the mechanism of traditional Chinese medicine （TCM） in the treatment of MPP based on gut-lung axis.MethodsSixty children with MPP of phlegm-heat blocking lung syndrome were randomly divided into a control group （n=30） and an observation group （n=30）； and 30 healthy children were recruited at the same time as a normal group. The control group was administered with azithromycin， and the observation group was administered with modified XBCQ together with the treatment of the control group. The treatment of both groups lasted 5d. We observed the clinical efficacy， collected feces samples from children in each group， and used real-time quantitative polymerase chain reaction to detect the number of Bifidobacterium， Lactobacillus， Ruminococcus flavefaciens and Clostridium butyricum， and enzyme-linked immunosorbent assay to detect the sIgA content. We used the Person correlation coefficient to describe the correlation between the number of intestinal microflora and the sIgA content in feces， and we also performed safety evaluation.Results①The total effective rate of the observation group was 93.33%， and that of the control group was 70.00 %. The clinical efficacy of the observation group was better than that of the control group （P<0.01）. ②The number of Bifidobacterium， Lactobacillus， Ruminococcus flavefaciens and Clostridium butyricum in feces was lower in children with MPP of phlegm-heat blocking lung syndrome than that in healthy children of the normal group （P<0.001）； after treatment， the number of Bifidobacterium， Lactobacillus， Ruminococcus flavefaciens and Clostridium butyricum increased in the observation group （P<0.001） and was higher than that in the control group （P<0.001）. ③Children with MPP of phlegm-heat blocking lung syndrome had lower fecal sIgA content than healthy children of the normal group （P<0.05）； after treatment， the fecal sIgA content in the observation group increased （P<0.001） and was higher than that of the control group （P<0.001）. ④The number of Bifidobacterium， Lactobacillus， Ruminococcus flavefaciens and Clostridium butyricum in feces of children with MPP of phlegm-heat blocking lung syndrome was positively correlated with the sIgA content （P<0.001）. ⑤During the course of treatment， no significant adverse reaction was observed in patients under treatment.ConclusionsSatisfactory results have been obtained among patients with MPP of the phlegm-heat blocking lung syndrome treated by modified XBCQ with conventional therapy. The mechanism is related to the increase of beneficial intestinal microflora and intestinal mucosal immunity regulation.关键词：mycoplasma pneumoniae pneumonia;phlegm-heat blocking lung syndrome;children;modified Xuanbai Chengqi Decoction;intestinal microflora;sIgA;clinical trial
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发布时间：2022-10-09 - 摘要：ObjectiveTo observe the clinical efficacy of Qizhi Tongluo Capsule in treating vascular cognitive impairment （VCI） of qi deficiency and blood stasis syndrome.MethodsNinety patients with VCI of qi deficiency and blood stasis syndrome were randomly divided into a treatment group （n=60） and a control group （n=30）， and both groups were given basic treatment. The treatment group was additionally administered with Qizhi Tongluo Capsule and the control group was given Qizhi Tongluo Capsule mimics. The treatment course was 24 weeks. The clinical efficacy was observed， and the changes in the TCM syndrome scores as well as the scores of the Mini-mental State Examination （MMSE）， the Clock Drawing Test （CDT） and the Hopkins Word Learning Test （HVLT） were compared. The safety evaluation was performed.Results①During the trial， there were 3 drop-out cases in the treatment group and 2 drop-out cases in the control group， and 85 cases finally completed the trial， including 57 cases in the treatment group and 28 cases in the control group. ②The total clinical effective rate of the treatment group was 94.74%， and that of the control group was 53.57%； the clinical efficacy of the treatment group was better than that of the control group （P<0.01）. ③The scores of qi deficiency syndrome scale and blood stasis syndrome scale decreased in both groups after treatment （P<0.01）； the scores of qi deficiency syndrome scale and blood stasis syndrome scale in the treatment group were lower than those in the control group （P<0.01）. ④The MMSE， CDT， and HVLT scores increased in the treatment group after treatment （P<0.05）； the differences in MMSE， CDT， and HVLT scores between the two groups were statistically significant after treatment （P<0.05）. ⑤No serious adverse reaction was observed in both groups during the trial.ConclusionQizhi Tongluo Capsule has achieved satisfactory results in treating vascular cognitive impairment of qi deficiency and blood stasis syndrome， and it can significantly improve the cognitive dysfunction and TCM syndromes of patients with good safety.关键词：vascular cognitive impairment;dementia;qi deficiency and blood stasis syndrome;Qizhi Tongluo Capsule;Buyang Huanwu Decoction;clinical trial
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发布时间：2022-10-09 - 摘要：ObjectiveTo observe the clinical efficacy of acupoint application of Ningxin Paste combined with western medicine in the treatment of ventricular extrasystole with syndrome of deficiency of heart and spleen.MethodsSeventy patients of ventricular extrasystole with syndrome of deficiency of heart and spleen were randomly divided into the control group and treatment group， 35 patients in each group. The control group was treated with propafenone hydrochloride， and the treatment group was treated with Ningxin Paste by acupoint application at bilateral Neiguan acupoints. The course of treatment in both groups was 4 weeks. The clinical efficacy was observed， and the changes of Chinese medical syndrome score， 24 h dynamic electrocardiogram （ECG） indexes and arrhythmia risk stratification were compared.Results①The total effective rates of Chinese medical syndrome in the treatment group and the control group were 88.57% and 60.00% respectively， and the total effective rates of ventricular extrasystole were 91.43% and 80.00% respectively. The efficacy of the treatment group was better than that of the control group （P<0.05， P<0.01）. ②Compared with those before treatment， the score of Chinese medical syndrome and the number of ventricular extrasystole were decreased in both groups after treatment （P<0.05）. After treatment， the score of Chinese medical syndrome and the number of ventricular extrasystole in the treatment group were less than those in the control group （P<0.05， P<0.01）. ③Changes of heart rate variability indexes ［overall standard deviation （SDNN）， average standard deviation （SDANN）， root mean square difference （RMSSD）， percentage of exceeding 50 ms （pNN50）］： Compared with those before treatment， the values of SDNN， SDANN， RMSSD and pNN50 were increased in both groups （P<0.05）. After treatment， the values of SDNN， SDANN， RMSSD and pNN50 in the treatment group were higher than those in the control group （P<0.05， P<0.01）. ④After the treatment， the risk stratification of arrhythmia was improved in both groups （P<0.05）， and the improvement in the treatment group was better than that in the control group （P<0.05）. ⑤During the treatment， there were no obvious adverse reactions in both groups.ConclusionsAcupoint application of Ningxin Paste combined with western medicine shows good efficacy in the treatment of ventricular extrasystole with syndrome of deficiency of heart and spleen. Compared with western medicine alone， acupoint application of Ningxin Paste combined with western medicine can better improve the clinical symptoms and prognosis of patients， with good safety.关键词：ventricular extrasystole;arrhythmia;syndrome of deficiency of heart and spleen;acupoint application;Guipi Dcoction;Neiguan acupoint;heart rate variability;clinical trial
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发布时间：2022-10-09 - 摘要：ObjectiveTo observe the changes of urodynamics and contractility of isolated detrusor strips at different time points after electroacupuncture （EA） in rats with detrusor overactivity （DO）， and provide a theoretical guidance and basis for the temporal dynamics in the effectiveness of clinical acupuncture treatment.MethodsOne hundred and sixty Wistar female rats were randomly divided into control， model， EA and drug groups， each with 40 rats， and each group was further randomly divided into 4 subgroups （corresponding to different observation time points： 2 h， 24 h， 48 h and 72 h after the intervention）， 10 rats in each subgroup. Except for the control group， all rats were prepared into DO model by intraperitoneal injection of cyclophosphamide. On the basis of the model， the EA group was given EA at Ciliao （BL32） and Huiyang （BL35） points， and the drug group was administered with imatinib mesylate by gavage. Rats in the control， model and drug groups were bound and fixed for 20 min as those in the EA group， and rats in the control， model and EA groups were administered with drinking water of the same body mass by gavage as those in the drug group， once a day for 3 consecutive days. The effective bladder capacity， urinary storage time and voiding pressure of the rats were analyzed at each observation time point by urodynamic detection technique. Changes in contractility of the isolated detrusor strips of the bladder were observed in each group using the isolated detrusor traction test.ResultsA total of 124 rats were finally included in the statistical analysis. After modeling， the effective bladder capacity of rats was significantly smaller and urinary storage time was significantly shorter in the model group than those of rats in the control group （P<0.05）. At the time points of 2 h， 24 h， 48 h and 72 h after intervention， the effective bladder capacity of rats was significantly larger and urinary storage time of rats was significantly longer in the EA group and the drug group than those of rats in the model group （P<0.05）， but the differences between the EA group and the drug group were not statistically significant （P>0.05）. At 24 h after intervention， the effective bladder capacity of rats was larger and urinary storage time of rats was longer in the EA group than those of rats observed at other three time points （P>0.05）. The contraction of isolated detrusor strips of rats in the model group had lower amplitude than that in the control group， while the contraction frequency was higher than that in the control group （P<0.05）. Compared with the contraction amplitude of detrusor strips of rats in the model group， the contraction amplitude in the EA group increased significantly at 24 h after intervention （P<0.05）， and the increase continued until 72 h after intervention， but there was no increase in the contraction amplitude in the drug group. Both EA and drug groups could reduce the contraction frequency of bladder detrusor strips in DO model rats， and the effect was optimal at 24 h after intervention （P<0.05）.ConclusionElectroacupuncture can effectively increase the effective bladder capacity and urinary storage time of DO rats， increase the contraction amplitude of isolated detrusor strips and stabilize the contraction frequency of detrusor strips. The effect of electroacupuncture lasts at least for 72 h after acupuncture， and the effect is optimal at 24 h after intervention.关键词：acupuncture therapy;electroacupuncture;overactive bladder;detrusor overactivity;urodynamics;rat model
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发布时间：2022-10-09 - 摘要：ObjectiveTo explore the effect and mechanism of Bushen Huanjing Recipe on sarcopenia mice.MethodsThe aged senescence accelerated mouse prone 8 （SAMP8） was used as a sarcopenia model， and 36 SAMP8 mice were randomly divided into 0.9%NaCl solution group （control group）， Bushen Huanjing Recipe group （Bushen group） and ferroptosis inhibitor Ferrostatin-1 （Ferr-1） intraperitoneal injection group （Ferr-1 group）. Gavage intervention was given in the control and the Bushen groups for 12 weeks， and inhibitor injection intervention was given for 3 weeks. After the intervention， the muscle mass of the mice was weighed. The muscle strength of the mice was detected by a muscle functional test system， and the maximum grip strength of the mice was detected by a grip strength meter. The microstructure of the mice muscle fibers was detected by the laminin immunofluorescence staining. The contents of reduced nicotinamide adenine dinucleotide phosphate （NAPDH）， glutathione （GSH）， malondialdehyde （MDA） and 4-hydroxynonenal （4-HNE） by immunohistochemistry staining were used to detect lipid oxidation in muscles of the mice. The expression of ferroptosis related proteins such as anti-glutathione peroxidase 4 （Gpx4）， SCL7A11， P53 and P21 in mice muscles was detected by Western blot， and the morphology of mitochondria of muscle fiber in the mice were detected by a transmission electron microscope.ResultsCompared with the control group， Bushen Huanjing Recipe and Ferr-1 intervention increased the muscle mass， strength and grip strength as well as the area of muscle fibers in the mice， improved the morphology of mitochondria in muscle fibers（P<0.01）， and reduced lipid peroxidation. The expression of antioxidant glutathione peroxidase （Gpx4） and SLC7A11， the key element of cystine / glutamate antiporter， was significantly increased （P< 0.001）， while the expression of cell senescence-related protein p53 and its downstream protein P21， which negatively regulated SLC7A11， was significantly decreased （P<0.001）.ConclusionThe Bushen Huanjing Recipe can effectively treat sarcopenia in mice by regulating ferroptosis.关键词：sarcopenia;Bushen Huanjing Recipe;mice model;ferroptosis;traditional Chinese herbal medicine research
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发布时间：2022-10-09 - 摘要：ObjectiveTo establish UPLC fingerprint and content determination methods of Codonopsis Radix dispensing granules （CRDG） and honey bran frying Codonopsis Radix dispensing granules （HBFCRDG），and to provide a comprehensive evaluation of the quality of CRDG and HBFCRDG.MethodsUPLC analysis was performed on a Waters ACQUITY UPLC HSS T3 column （2.1 mm×100 mm，1.8 μm），with a mobile phase consisting of acetonitrile （A） - 0.1% formic acid （B） at the flow rate of 0.3 mL·min-1. The column temperature was controlled at 30 ℃ and the detection wavelength was set at 268 nm. The injection volume was 3 μL.ResultsUPLC fingerprint and content determination methods of HBFCRDG were established. Lobetyolin displayed ideal linearity within the range of 2.4-100.0 mg·L-1 （R2=0.999 7）. The recoveries of lobetyolin in three levels of 50%，100% and 150% were 97.72%， 96.23%， 98.60% respectively，and the total average recovery was 97.52% with RSD of 1.76%. The RSD values of precision，repeatability and stability were all less than 3%. Nine common peaks were designated in fingerprint and peaks of lobetyolin，tangshenoside Ⅰ，lobetyolinin and lobetyol were confirmed by standard references. The content of lobetyolin in 28 batches of CRDG and HBFCRDG was between 0.06-0.39 mg·g-1. According to the results of PCA and PLS-DA，HBFCRDG can be distinguished from CRDG obviously. What’s more，it showed that there were differences in samples of CRDG from different manufacturers.ConclusionThe method has the advantages of simple operation， high detection efficiency and strong specificity， and can be used for the quality evaluation of CRDG and HBFCRDG.关键词：Codonopsis Radix;honey bran frying Codonopsis Radix;dispensing granules;UPLC;traditional Chinese herbal medicine research
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发布时间：2022-10-09 - 摘要：ObjectiveTo explore the effect of Xifeng Zhidong Decoction on inflammatory reaction of mouse microglia （BV-2 cells） induced by lipopolysaccharide （LPS）.MethodsThe microglia of mice were divided into three groups： control group， LPS group and Xifeng Zhidong Decoction group. The DMEM medium was added to the control group. LPS was used to induce inflammation on microglia at a concentration of 0.1 mg·L-1 in the LPS group， and intervention was performed by Xifeng Zhidong Decoction with a concentration of 12.5， 25， 50， 100， 200 and 400 mg·L-1 respectively in the Xifeng Zhidong Decoction group. The effect of Xifeng Zhidong Decoction on the viability of microglia was observed by cell proliferative toxicity test （CCK-8）. The level of nitric oxide （NO） in the supernatant was detected by enzyme linked immunosorbent assay （ELISA）， and the expression of inflammatory factors in microglia was detected by fluorescence quantitative polymerase chain reaction （real-time PCR）. Western blot and immunofluorescence staining were used to detect the expression of nitric oxide-induced synthase （iNOS） and arginine-1 （Arg-1） proteins.ResultsThere was no significant effect on the activity of microglia in different doses of Xifeng Zhidong group or LPS group （P>0.05）. Compared with the control group， the NO content in microglia supernatant of the LPS group increased significantly（P<0.001）； the mRNA expression of TNF-α， IL-6， IL-1β of M1 type microglia increased significantly（P<0.001）， but the mRNA expression of TGF-β of M2 type microglia decreased （P<0.05） ； the protein expression of M1 marker iNOS increased （P<0.01）， but the expression of M2 marker Arg-1 protein decreased （P<0.05）. Compared with the LPS group， Xifeng Zhidong Decoction at 100， 200， and 400 mg·L-1 could significantly inhibit the release of NO（P<0.001）， decrease the expression of TNF-α， IL-6 mRNA of M1 type microglia（P<0.001， P<0.05）. Xifeng Zhidong Decoction increased the expression of IL-10 and TGF-β mRNA of M2 type microglia at 400 mg·L-1（P<0.05）， reduced the expression of M1 type marker iNOS protein at 200 mg·L-1 （P<0.01）， and increased the expression of M2 type marker Arg-1 protein at 100 mg·L-1 （P<0.05）.ConclusionXifeng Zhidong Decoction can inhibit the transformation of microglia to M1 damaged type induced by LPS， and promote the transformation to M2 repair type.关键词：tic disorder;Xifeng Zhidong Decocton;microglial;lipopolysaccharide;inflammatory reaction;traditional Chinese herbal medicine research
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发布时间：2022-10-09 - 摘要：ObjectiveTo study the effect of nanoliposomal aerosol inhalation of Guben Pingchuan Formula （here in after referred to as Guben Pingchuan Inhaler） on immunity and gene expression in asthmatic guinea pigs.MethodsForty-eight guinea pigs were divided into six groups： normal control group， asthma model group， traditional Chinese medicine （TCM） control group， Guben Pingchuan inhaler low-dose group （hereinafter referred to as low-dose group）， Guben Pingchuan Inhaler high-dose group （hereinafter referred to as high-dose group）， and western medicine control group. All the groups except for the normal group received an intraperitoneal injection of 10% ovalbumin in 0.9% NaCl solution to make the guinea pig model. Asthma model group， TCM control group， low-dose group， high-dose group， and western medicine control group inhaled 0.9% NaCl solution， Xiaoqinglong Mixture， nano-liposome aerosol inhaler of Guben Pingchuan Formula， and dexamethasone sodium phosphate， respectively. ELISA was used to detect allergy-related immunological indicators ［interleukin-4 （IL-4）， interleukin-5 （IL-5）， interleukin-12 （IL-12）， interleukin-25 （IL-25）， interferon γ （IFN-γ）］； RT-PCR technique was used to detect signal transducer and activator of transcription 6 （STAT6） and tumor necrosis factor-α （TNF-α） expression； HE staining was used to observe lung histopathology.ResultsThe serum levels of IL-4， IL-5 and IL-25 in the asthma model group were higher and the levels of IL-12 and IFN-γ were lower than those in the normal control group， and the differences were statistically significant （P<0.05）. The serum levels of IL-4 and IL-5 were lower and the serum levels of IL-12 and IFN-γ were higher in each drug intervention group than those in the asthma model group. Besides， the levels of IL-25 were lower in the TCM control group， low-dose group and western medicine control group than that in the asthma model group， and the differences were statistically significant （P<0.05）. The expression of STAT6 and TNF-α mRNA was higher in the asthma model group than that in the normal control group， and the differences were statistically significant （P<0.05）. The expression of STAT6 and TNF-α mRNA was lower in both the low-dose and high-dose groups than that in the asthma model group and the TCM control group， and the differences were statistically significant （P<0.05）. The expression of STAT6 and TNF-α mRNA was lower in the low-dose group and the expression of TNF-α mRNA was lower in the high-dose group than that in the western medicine control group， and the differences were statistically significant （P<0.05）. Lung histopathology showed： the normal control group presented thickened pulmonary artery walls and mild inflammation due to nebulization and other factors； the asthma model group presented the most severe inflammatory condition and thickened arterial walls； the TCM control group presented alleviated inflammatory condition and proliferation of granulation tissue； the western medicine control group presented the destruction of lung tissue structure； the low-dose group presented thickened pulmonary artery walls， a small amount of secretion in the lumen of fine bronchi and 3 cases of mild/localized interstitial pneumonia； and the high-dose group presented thickened pulmonary artery walls， mild interstitial pneumonia， 2 cases of localized lung tissue destruction， and proliferation of granulation tissue.ConclusionGuben Pingchuan Inhaler showed some improvement in immunological indicators （IL-4， IL-5， IL-12， IL-25， IFN-γ）， expression of STAT6 and TNF-α mRNA， and lung histopathology in asthmatic guinea pigs.关键词：asthma;Guben Pingchuan Formula;aerosol inhalation;guinea pig;immunology;gene;traditional Chinese herbal medicine research
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发布时间：2022-10-09 - 摘要：ObjectiveTo improve the TLC identification of Zhuling Jianpi Capsule.MethodsThe supernatants of the inner content of Zhuling Jianpi Capsule via successive ultrasonication with n-hexane following with mehanol were separately used for the identification of Atractylodis Macrocephalae Rhizoma and for the identifications of Paeoniae Radix Alba， Phellodendri Chinensis Cortex and Citri Reticulatae Pericarpium in Zhuling Jianpi Capsule. The residual methanol filtrate was evaporated to dryness and dissolved with water， applied to D101 macroporous resin column （inner diameter 2 cm， column length 15 cm）， eluted successively with 10% methanol， 50% methanol， 80% methanol and 90% methanol. The 50% methanol eluate was used for identification of Linderae Radix and Saposhnikoviae Radix， 80% methanol eluate was used for identification of Glycyrrhizae Radix et Rhizoma， and 90% methanol eluate for identification of Magnoliae Officinalis Cortex.ResultsEight TLC identification methods were accomplished， and three TLC identification methods for Saposhnikoviae Radix， Linderae Radix and Glycyrrhizae Radix et Rhizomawere were newly established and three TLC identification methods for Magnoliae Officinalis Cortex， Phellodendri Chinensis Cortex and Citri Reticulatae Pericarpium were improved. Paeoniflorin was used as the marker of Paeoniae Radix Alba， norisoboldine as the marker of Linderae Radix， prim-O-glucosylcimifugin and 5-O-methylvisammioside as the markers of Saposhnikoviae Radix， and the mixture of chloroform and methanol （4∶1） was used as the same mobile phase for three of them； berberine hydrochloride was used as the marker of Phellodendri Chinensis Cortex and hesperidin as the marker of Citri Reticulatae Pericarpium， and the low-layer solution of the mixture of chloroform， methanol and water （37∶17∶5） was used as the same mobile phase for both of them； Magnolol and honokiol were used as the markers of Magnoliae Officinalis Cortex and the mixture of toluene and methanol （10∶1） as the mobile phase； liquiritin and monoammonium glycyrrhizinate were used as the markers of Glycyrrhizae Radix et Rhizoma， and the mixture of ethyl acetate， formic acid， glacial acetic acid， and water （15∶1∶1∶2） as the mobile phase.ConclusionA systematic sample test solution preparation strategy is implemented for concurrently preparing 4 test solutions for eight TLC identifications， the modified TLC identification methods are more suitable to control the quality of Zhuling Jianpi Capsule.关键词：irritable bowel syndrome;Zhuling Jianpi Capsule;TLC identification;quality standard;traditional Chinese herbal medicine research
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发布时间：2022-10-09 - 摘要：Depression is one of the most common non-motor symptoms in patients with Parkinson’s disease， which seriously affects the quality of life of patients. At present， there is no specific treatment available. More attention has been directed to the traditional Chinese medicine （TCM） research in this field. In order to provide reference for the clinical diagnosis and treatment of depression in Parkinson’s disease， we have reviewed the research progress on TCM treatment of depression in Parkinson’s disease in recent years from three aspects： traditional Chinese herbal medicine treatment， integrated Chinese and Western medicine therapy， and TCM non-pharmacological therapy.关键词：Parkinson’s disease;depression;non-motor symptoms;traditional Chinese medicine therapy;integrated Chinese and Western medicine therapy;syndrome differentiation and treatment;research progress
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发布时间：2022-10-09
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