最新刊期
期 6 ， 2022
- 关键词：COVID-19;SARS-CoV-2;Omicron variant;elderly;traditional Chinese medicine therapy;expert consensus
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发布时间：2022-07-13 - 摘要：Acupuncture and moxibustion （AM） medicine is rooted in Chinese culture and has unique cultural connotations， theoretical systems and technical methods. As an important part of traditional Chinese medicine （TCM）， AM medicine shoulders the dual tasks of inheritance and innovative development. The change of clinical research mode from retrospective research to prospective research， and the change of experimental research mode from confirmatory research to exploratory research， are the necessary ways to realize the transformation of AM medicine from empirical medicine to evidence-based medicine. The construction of an “academic ecosystem of acupuncture and moxibustion data resources” and a scientific evidence-based modern theoretical system of acupuncture and moxibustion medicine is the main content and goal of the “Acupuncture and Moxibustion Big Science Project”， and also the inevitable trend of the development of AM medicine itself.关键词：acupuncture and moxibustion;modern acupuncture and moxibustion medicine;inheritance;innovation
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发布时间：2022-07-13 - 摘要：ObjectiveBased on the “National Standardization Development Program” （here in after referred to as “Program”）， we analyzed the current status of acupuncture and moxibustion standardization in China， investigated the existing problems， put forward countermeasures and suggestions， and planned our future work. Great progress has been made in the standardization of acupuncture and moxibustion in China： the standard system of acupuncture and moxibustion has been established basically， a linkage working mechanism has been initially established among various institutions； and as for the international standardization work， China is changing its role from a passive participator to an active leader. However， there are still some problems： important standards are unavailable or outdated， and the compatibility of published standards needs to be strengthened； Practitioners’ awareness of standardization needs to be improved， talents are in short supply， research institutions are insufficient， and the management and operation mechanism of standards is not well organized. In the future， we should further improve the acupuncture and moxibustion standard system， supply important standards oriented by problems and needs， enhance the compatibility of standards， establish and improve the integration standards of artificial intelligence， big data and acupuncture and moxibustion. Meanwhile， we should also strengthen the construction of acupuncture and moxibustion standard support system， enhance standardization awareness， cultivate talents who master the knowledge of acupuncture， standardization， international cooperation and exchange rules and ancient Chinese language， expand the number and scale of research institutions， and improve the standard management and operation mechanism. Furthermore， we should actively participate in international standardization activities of acupuncture and moxibustion， promote mutual recognition of Chinese and foreign standards and foster the bidirectional opening when adopting international standards and keeping consistent with foreign standards.关键词：National Standardization Development Program;acupuncture and moxibustion;standardization;national standard;international standard;research status;countermeasure
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发布时间：2022-07-13 - 摘要：ObjectiveTo analyze the prescription and medication patterns of Professor Sun Shidao in treating skin diseases and summarize his academic thoughts.MethodsThe cases of skin diseases treated by Professor Sun Shidao were collected and a medical record database was established. The statistical software SPSS 22.0 was used for frequency analysis， and Apriori algorithm of IBM SPSS Modeler 18.0 was used for association rules and network analysis to mine the prescription and medication patterns.Results①A total of 7，289 patients involving 5，239 prescriptions and 402 traditional Chinese herbal medicines （TCHMs） were included. There were 346 disease names in modern medicine and 223 disease names in traditional Chinese medicine （TCM） ［The number of patients suffering from dermatitis eczema （damp sore） was the highest， followed by the number of patients with lupus erythematosus （red butterfly sore）， acne （comedo）， and psoriasis （white tinea）］. ②The drugs commonly used by Prof. Sun Shidao for treating skin diseases included Mudanpi （Moutan Cortex）， Danshen （Salviae Miltiorrhizae Radix et Rhizoma）， Dihuang （Rehmanniae Radix）， Xuanshen （Scrophulariae Radix）， Zhimu （Anemarrhenae Rhizoma）， Huangqin （Scutellariae Radix）， Tufulin （Smilacis Glabrae Rhizoma） and Baihuasheshecao （Hedyotis Diffusae Herba）. By analyzing the classification of diseases and TCM syndromes， it was found that Professor Sun had adopted different methods for different diseases and syndromes. When treating acne， he used the method of clearing heat and dampness as the main method， taking into account nourishing yin and reducing fire； When treating lupus erythematosus， he used the methods of nourishing yin and benefiting qi， cooling blood and nourishing blood； When treating psoriasis， he focused on the treatment of blood， putting equal emphasis on the methods of cooling blood， nourishing yin and invigorating blood. ③According to the results of association rule analysis， Professor Sun commonly used 20 TCHMs for the treatment of skin diseases， including Baihuashesehcao （Hedyotis Diffusae Herba）， Difuzi （Kochiae Fructus）， Kushen （Sophorae Flavescentis Radix）， Shanyao （Dioscoreae Rhizoma）， Baizhu （Atractylodis Macrocephalae Rhizoma）， Xuanshen （Scrophulariae Radix）， Yuzhu （Polygonati Odorati Rhizoma）， Baixianpi （Dictamni Cortex）， Dihuang （Rehmanniae Radix） and Tufulin （Smilacis Glabrae Rhizoma）. Among them， Dihuang （Rehmanniae Radix）， Tufulin （Smilacis Glabrae Rhizoma）， Baixianpi （Dictamni Cortex）， Yuzhu （Polygonati Odorati Rhizoma） and Mudanpi （Moutan Cortex） showed the closest network relationship with other TCHMs. According to the network analysis， there were 6 medicinal groups containing 7 TCHMs， among which Baixianpi （Dictamni Cortex）， Pugongying （Taraxaci Herba） and Baihuasheshecao （Hedyotis Diffusae Herba） were strongly correlated.ConclusionsProfessor Sun Shidao specializes in the treatment of dermatitis eczema （damp sore）， lupus erythematosus （red butterfly sore）， acne （comedo） and psoriasis （white tinea）. He often uses the method of clearing heat as the basis， and combines it with other methods such as wind-dispelling method， dampness-relieving method， blood-cooling method and yin-nourishing， etc. He is good at using the medication group of Baixianpi （Dictamni Cortex）， Pugongying （Taraxaci Herba） and Baihuasheshecao （Hedyotis Diffusae Herba）， etc.关键词：skin disease;dermatitis eczema;acne;lupus erythematosus;psoriasis;data mining;traditional Chinese herbal medicine research
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发布时间：2022-07-13 -
Modern mechanisms of diaphoretic therapy for psoriasis from the perspective of body fluid metabolism
摘要：Psoriasis vulgaris is a chronic， inflammatory， relapsing skin disorder mediated by immunity under the background of polygenic inheritance， and mainly characterized by the presence of erythema， scales and plaques. The common type is the most frequently seen in the clinic. Diaphoresis as the leading therapy of the eight treatment methods in traditional Chinese medicine （TCM） is also a common method used in the treatment of psoriasis. The main pathogenesis of psoriasis lies in the depressed yang and blood heat as sweat pores are obstructed and blocked by the exterior cold dampness. Therefore， diaphoretic therapy might be the key to the treatment of psoriasis. Besides， it also has a solid clinical application foundation. Our research group has proposed that psoriasis being severe in winter and mild in summer may be related to the metabolism of body fluids. Single molecule array （SIMOA） technology has provided a new detection method for metabolic body fluids such as sweat and urine. For instance， sweat could be collected with a non-invasive sweat patch and then inflammatory cytokines in sweat could be detected via SIMOA technology. After sweat component analysis we could explore the related mechanisms. The in-depth study of the role that body fluid metabolism plays in the relapsing of psoriasis may bring new approaches and insights into the study of the working mechanism in the TCM clinical treatment of psoriasis.关键词：psoriasis;diaphoretic therapy;body fluid metabolism;obstructed and blocked sweat pore;inflammatory cytokine;single molecule array technology- 39
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发布时间：2022-07-13 - 摘要：The purpose of this paper is to introduce Professor Qin Wanzhang’s clinical experience in the treatment of allergic dermatoses. It is suggested that the main cause of allergic dermatoses is pathogenic wind， dampness and heat. The routine treatment is dispelling wind， clearing heat and disinhibiting dampness and the main prescription is Mahuang Lianqiao Chixiaodou Decoction. Based on the combination of disease differentiation and syndrome differentiation， comprehensive treatment can be flexibly applied， such as activating blood and resolving stasis， nourishing blood to moisten dryness， releasing depression to tranquilize spirit， relieving itching by heavy settling， fortifying the spleen and tonifying the lung， replenishing qi and enriching yin and so on. And two proven cases are presented.关键词：allergic dermatoses;Mahuang Lianqiao Chixiaodou Decoction;traditional Chinese medicine therapy;Qin Wanzhang
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发布时间：2022-07-13 - 摘要：This paper introduces professor Li Bin’s experience in the treatment of chronic skin ulcers based on the theory of “mutual binding of dampness and stasis”. It is concluded that the pathogenesis of chronic skin ulcer is mainly the blockage of meridian， qi and blood congealing and stasis， gathering into dampness， resulting in mutual binding of dampness and stasis， which blocking the blood vessels and causing the disease. In the early stage， the treatment mainly focuses on fortifying the spleen and eliminating dampness， resolving blood stasis to promote tissue regeneration， and tonifying and replenishing qi and blood in the late stage. When prescribing drugs， Professor Li pays equal attention to dispelling stasis and resolving dampness， and is good at using insect drugs and rattan drugs. And one proven case is presented.关键词：chronic skin ulcers;mutual binding of dampness and stasis;moving qi and dispelling stasis;insect drugs;Sargentodoxae caulis;traditional Chinese medicine therapy
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发布时间：2022-07-13 - 摘要：The purpose of this paper is to summarize the clinical experience of Professor Ouyang Weiquan in the treatment of hormone dependent dermatitis. Professor Ouyang thinks that hormone dependent dermatitis usually presents syndrome of cold and heat complex， suggesting that hormone dependent dermatitis should be treated with Linggan Wuwei Jiangxin Xiaxing Plus Dahuang Decoction and Danggui Shaoyao Powder according to the patients’ constitution of cold dampness and the characteristics of skin lesions. And two proven cases are presented.关键词：hormone dependent dermatitis;classic prescription;Ouyang Weiquan;traditional Chinese medicine therapy
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发布时间：2022-07-13 - 摘要：Through the syndrome differentiation of the viscera and meridians， combined with the systematic analysis of modern medicine and the medical cases summary， the symptoms， pathogenesis and treatments of Parkinson’s disease were discussed， and the core pathogenesis of “five viscera deficiency， the Taiyang meridian blocking” and the treatment of "adjusting the five viscera and dredging the Taiyang meridian " were established.关键词：Parkinson’s disease;viscera state;viscera;meridian;syndrome differentiation;pathogenesis;traditional Chinese medicine therapy
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发布时间：2022-07-13 - 摘要：This paper discussed the attribution of “Xuefu” in viscera organ from three aspects： the proposal and development of “Xuefu”， the essence of “Xuefu”， and the significance of liver as “Xuefu”. Ancient doctors debated whether “Xuefu” was actually in the heart， blood vessel or chest. The production of blood depended on the transportation and transformation of the spleen and stomach， and the qi of the heart and lungs promoted the blood flowing. Vessels were actual channels for blood to circulate， not storage， only the liver could store blood and regulate blood circulation， and it was an actual treasury. The theory of “Xuefu” created by Wang Qingren was wrong， but “Xuefu Zhuyu Decoction” had excellent curative effects， the reason was that he was limited by the anatomical conditions at that time. Judging from the fact that this formula was actually composed of two functional units： Sini San （soothing the liver ） and Taohong Siwu Decoction （invigorating blood circulation）， the key to its effect was regulating the liver， so the exact location of “Xuefu” was the liver， rather than the heart， blood vessels or chest.关键词：Xuefu;textual research;Xuefu Zhuyu Decoction;formula syndrome;liver storing blood;viscera
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发布时间：2022-07-13 - 摘要：ObjectiveTo observe the clinical efficacy of Feiyanning Granule in treating the intermediate and advanced stage lung squamous cell carcinoma （LSCC） of male patients with essence and qi deficiency syndrome.MethodsSixty male patients with intermediate and advanced stage LSCC of essence and qi deficiency syndrome were randomly divided into a treatment group （n=30） and a control group （n=30）. The treatment group was treated with Feiyanning Granule， while the control group was treated with Feiyanning Granule simulant. The course of treatment of both groups lasted 2 months. We observed the clinical efficacy， and compared the changes of Traditional Chinese Medicine （TCM） syndrome score， Karnofsky Performance Status （KPS） score， serum tumor markers and related immune indexes.Results①After treatment， the disease control rate of the treatment group was 80.0% and that of the control group was 56.7%. The clinical efficacy of the treatment group was better than that of the control group （P<0.05）. ②According to the intra-group comparison before and after treatment， the scores of fatigue， cough， hemoptysis， pain and insomnia in the treatment group decreased （P<0.05）， while there was no significant difference in the above-mentioned TCM syndrome scores in the control group （P>0.05）. After treatment， the scores of fatigue， cough， hemoptysis， pain and insomnia in the treatment group were lower than those in the control group （P<0.05）. ③The KPS score of the treatment group was better than that of the control group （P<0.05）. ④The intra-group comparison before and after treatment showed that the levels of cancer antigen 12-5 （CA12-5）， cytokeratin 19 fragment antigen 21-1 （CYFRA21-1） and squamous cell cancinoma associated antigen（SCC） in the treatment group decreased （P<0.05）； there was no significant difference in the level of tumor markers in the control group （P>0.05）； after treatment， the levels of CA125， CYFRA21-1 and SCC in the treatment group were lower than those in the control group （P<0.05）. ⑤There was no significant difference in the immune indexes between the two groups before and after treatment （P>0.05）； After treatment， the levels of CD4 + and NK cells in the treatment group were higher than those in the control group （P<0.05）.ConclusionFeiyanning Granule is effective in the treatment of intermediate and advanced stage lung squamous cell carcinoma of male patients with essence and qi deficiency syndrome， as it can effectively improve the clinical symptoms and immune function， enhance the quality of life， reduce serum levels of tumor markers and inhibit tumor growth.关键词：lung squamous cell carcinoma;intermediate and advanced tumor;essence and qi deficiency syndrome;Feiyanning;clinical trial
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发布时间：2022-07-13 - 摘要：ObjectiveTo observe the clinical efficacy of treating-winter-diseases-in -summer combined with doxazosin mesylate tablets in the treatment of benign prostatic hyperplasia（BPH）.MethodsA total of 108 patients with BPH were randomly divided into two groups， the treatment group and the control group， 54 cases in each group. The control group received doxazosin mesylate tablets， while the treatment group received doxazosin mesylate tablets combined with treating-winter-diseases-in-summer（moxibustion and acupoint application in the dog days）. The treatment course of both groups was in the dog days（about 6 weeks in summer）of 2018， 2019 and 2020 . The changes of international prostate symptom score （IPSS）， quality of life score （QOL）， TCM syndrome score， maximum urine flow rate （Qmax）， and prostate volume （PV） in two groups were observed before and after treatment. IPSS， QOL， and TCM syndrome scores were followed up in the winter of the same year.Results①A total of 98 patients completed the treatment， including 50 cases in the treatment group and 48 cases in the control group. ②The total effective rate was 72.00% in the treatment group and 56.35% in the control group. The clinical efficacy of the treatment group was better than that of the control group （P<0.05）. ③After treatment， IPSS， QOL， TCM syndrome score， and Qmax in two groups were significantly improved compared with before treatment （P<0.05）. The treatment group was better than the control group in improving QOL， TCM syndrome score， and Qmax （P<0.05）. ④During the period of follow up， IPSS， QOL， and TCM syndrome scores of the two groups were significantly improved compared with those before treatment （P<0.05）， QOL and TCM syndrome scores were significantly improved compared with those after treatment （P<0.05）； There was no significant difference in IPSS between follow-up and after treatment in the treatment group（P>0.05）， while there was a significant difference in IPSS between follow-up and after treatment in the control group （P<0.05）； The improvement of IPSS， QOL， and TCM syndrome score in the treatment group was better than that in the control group （P<0.05）. ⑤There was no significant difference in the incidence of adverse reactions between the two groups （P>0.05）.ConclusionsTreating-winter-diseases-in -summer combined with doxazosin mesylate tablets in the treatment of BPH can improve clinical efficacy， reduce IPSS， improve the urination symptoms， and improve the quality of life of patients. The treatment is safe and lasting for a while.关键词：benign prostatic hyperplasia;treating-winter-diseases-in -summer;doxazosin mesylate;clinical trial
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发布时间：2022-07-13 - 摘要：ObjectiveTo observe the clinical effect of Bufei Yishen Decoction combined with conventional western medicine on patients with moderate or severe chronic obstructive pulmonary disease （COPD） in stable stage and with lung-kidney deficiency syndrome， and investigate its influence on the level of serum inflammatory factors of patients.MethodsTotally 120 patients with moderate or severe COPD in stable stage and with lung-kidney deficiency syndrome were randomly divided into a treatment group （n=60） and a control group （n=60）. The control group was treated with conventional western medicine， while the treatment group was treated with Bufei Yishen Decoction besides the treatment measures of the control group. The course of treatment of both groups was 3 months， and the patients were followed up for 1 year after treatment. The clinical effect was observed， the changes of TCM syndrome scores， pulmonary function indexes， the 6-min walking test （6MWT）， chronic obstructive pulmonary disease assessment test （CAT） score， annual acute exacerbation frequency and serum levels of inflammatory factors were compared， and the safety evaluation was also conducted.Results①The total effective rate of treatment group was 83.33% and that of control group was 31.67%， and the treatment group showed more significant clinical efficacy than the control group （P<0.01）. ②After treatment and at the end of follow-ups， the TCM syndrome scores in the treatment group decreased （P<0.05， P<0.01）， while the TCM syndrome scores in the control group increased （P<0.05）. After treatment and at the end of follow-ups， TCM syndrome scores in the treatment group were comparatively lower than those in the control group （P<0.05）. ③After treatment， the frequency of acute exacerbations in the treatment group decreased （P<0.01） and was lower than that in the control group （P<0.05）. ④According to the intra-group comparison before and after treatment， there was an increase in the forced expiratory volume in the first second （FEV1） and 6MWT in the treatment group after treatment （P<0.05， P<0.01）， while the pulmonary function indexes and 6MWT in the control group did not change significantly after treatment （P>0.05）. Compared with the condition before treatment， there was an increase in the 6MWT in the treatment group at the end of follow-ups （P<0.05）， while the lung function indexes and 6MWT in the control group had no significant change （P>0.05）. After treatment and at the end of follow-ups， FEV1， FEV1/FVC and 6MWT in the treatment group were higher than those in the control group （P<0.05）. ⑤Compared with the CAT scores before treatment， the CAT scores in the two groups were lowered after treatment and at the end of follow-ups （P<0.01）； The CAT scores in the treatment group were lower than those in the control group after treatment and at the end of follow-ups （P<0.05）. ⑥After treatment， the level of IL-10 in serum increased （P<0.05）， but the IL-17 and TNF-α levels in serum decreased in the treatment group （P<0.01）. There were significant differences in serum IL-10， IL-17 and TNF-α levels between the two groups after treatment （P<0.05， P<0.01）. ⑦During the trial， there was no significant adverse reaction in the two groups.ConclusionBufei Yishen Decoction combined with conventional western medicine is effective in treating patients with moderate or severe COPD in stable stage and with lung-kidney deficiency syndrome， because it can effectively reduce the frequency of acute exacerbations， improve the lung function， exercise endurance and quality of life of patients， and its mechanism may be related to the regulation of serum inflammatory factors.关键词：chronic obstructive pulmonary disease;stable stage;Bufei Yishen Decoction;integrated traditional Chinese and Western medicine therapy;inflammatory factor;clinical trial
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发布时间：2022-07-13 - 摘要：ObjectiveTo observe the effects of acupuncture combined with Bushen Huoxue Prescription in the treatment of ovulatory dysfunction infertility of kidney deficiency and blood stasis type.MethodsA total of 90 ovulatory infertility patients were randomly divided into acupuncture-medication group，traditional Chinese medicine （TCM） group and control group， with 30 cases in each group.The control group was only treated with ovulation induction， the TCM group was treated with TCM combined with ovulation induction， and the acupuncture-medication group was treated with acupuncture in addition. Each group was treated with 1~3 menstrual cycles. The occurrence of ovulation， pregnancy and ovarian hyperstimulation（OHSS），luteinization （LUFS） and unresponsive cycle were compared among groups.Results① Compared with the other two groups， the acupuncture group used less HMG periodic， while the TCM group was between the other two groups， and the difference among those was statistically significant （P<0.05）. ②Cycle ovulation rate of both traditional Chinese medicine group and acupuncture-medication group were significantly higher than that of the control group（P<0.05），but there was no significant difference between the traditional Chinese group and the acupuncture-medication group （P>0.05）. ③The pregnancy cycles of acupuncture-medication group were significantly better than those of the control group（P<0.05），but there was no significant difference between the acupuncture-medication group and the TCM group（P>0.05）， and no significant difference between the TCM group and the control group （P>0.05）. ④There was no significant difference in the incidence of OHSS cycle and LUFS cycle among the three groups （P>0.05）. ⑤The anovulatory cycles of the acupuncture group were less than the control group（P<0.05）.There was no significant difference between acupuncture-medication group and the TCM group（P>0.05），also between the TCM group and the control group （P>0.05）.ConclusionAcupuncture-medication group can significantly reduce the use of HMG cycle， increase the follicular development cycle， reduce anovulatory cycle and improve the pregnancy cycle rate.关键词：infertility;ovulation disorder;syndrome of kidney deficiency and blood stasis;acupuncture;Bushen Huoxue Prescription;clinical trial
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发布时间：2022-07-13 - 摘要：ObjectiveTo investigate the analgesic effect of buccal acupuncture therapy during patient positioning before anesthesia for hip fracture surgery.MethodsSixty patients with hip fractures to be treated surgically were randomly divided into a treatment group （n=30） and a control group （n=30）. The patients in both groups would undergo combined spinal-epidural anesthesia. Before patient positioning for intraspinal anesthesia， the treatment group was administered with buccal acupuncture analgesia， and the control group was administered with sufentanil intravenously. The pain visual analogue scale （VAS） score changes at the time of patient entering the operating room （T0）， before patient positioning （T1） and after patient positioning （T2） were observed. The time spent in patient positioning prior to anesthesia， the time taken to complete anesthesia， and the time taken for the temperature sensation at the surgical site to disappear， as well as the incidence of adverse reactions from the time after analgesic therapy to the time before surgery were compared between groups.Results①All cases completed the trial. ②The VAS scores of patients in both groups decreased significantly at T1 and T2 compared with those at T0 （P<0.05）； the VAS scores of patients in both groups increased significantly at T2 compared with those at T1 （P<0.05）. The VAS scores of the treatment group were significantly lower than those of the control group at T1 and T2 （P<0.05）. ③The time spent in patient positioning prior to anesthesia in the treatment group was significantly shorter than the time spent in the control group， and the time spent to complete anesthesia in the treatment group was also shorter （P<0.05）. There was no statistically significant difference in the time taken for the loss of temperature sensation at the surgical site between the two groups （P>0.05）. ④The incidence of adverse reactions in the treatment group was significantly lower than that in the control group from the time after analgesic treatment to the time before surgery （P<0.05）.ConclusionBuccal acupuncture therapy can provide effective analgesic effects for hip fracture patients during positioning prior to intraspinal anesthesia. Compared with the use of intravenous analgesic drugs， it is easier to administer， with less adverse reactions and higher safety evaluation.关键词：hip fracture;buccal acupuncture therapy;microneedle;acupuncture analgesia;perioperative period;clinical trial
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发布时间：2022-07-13 - 摘要：ObjectiveTo observe the effect of electroacupuncture on the decomposition and synthesis of collagen in anterior vagina wall of rats with stress urinary incontinence （SUI）.MethodsTotally 60 SD rats were randomly divided into sham operation group （n=10） and model group （n=50）. The sham operation group was treated by laparotomy， and the ovarian tissue was found and sutured directly without ovariectomy and vaginal dilatation； the model group was established by bilateral ovariectomy after vaginal dilatation， and the sneeze test was used to test the success of the model. The successful model rats were randomly divided into the model group， the electroacupuncture non-acupoint group （“acupuncture non-acupoint group”）， the electroacupuncture group and the blocker group， and the model group did not receive any treatment. The rats in the electroacupuncture group were treated with electroacupuncture at bilateral “Shenshu（BL23）” and “Huiyang（BL35）” points for 20 min each time for 3 days. The rats in the acupuncture non-acupoint group were treated with acupuncture at the control points of “Shenshu（BL23）” and “Huiyang（BL35）” （1 cm beside the two acupoints） and electroacupunctured for 20 min each time for 3 days， while those in the blocker group were given doxycycline hydrochloride intragastrically for 3 days. After treatment， urodynamic indexes were evaluated， and the mRNA and protein expression of total collagen content （Col-Ⅰ， Col-Ⅲ）， degradation link related factors （MMP-1， MMP-2， MMP-9， TIMP-1）， collagen synthesis link related factors （DCN， TGF-β） in anterior vagina wall were detected by PCR， Western blot and immunohistochemistry.Results① After modeling， the positive sneezing test indicated that the SUI model was successful. Compared with the sham operation group， the maximum bladder capacity and abdominal pressure leakage point pressure of the model rats were significantly lower （P<0.05）. ② After treatment， compared with the sham operation group， the expression levels of Col-I， Col-Ⅲ mRNAs and proteins， TGF-β mRNA， and TIMP-1 mRNA and protein increased（P<0.05）， while the expression levels of MMP-1， MMP-2， MMP-9 mRNAs and proteins， and DCN mRNA and protein decreased（P<0.05）in the model group. ③ Compared with the model group， the leak point pressure and the maximum capacity of bladder in the electroacupuncture group and the blocker group were significantly higher than those in the model group （P<0.05）. In the electroacupuncture group， the expressions of Col-I， Col-Ⅲ mRNAs and proteins， TIMP-1 mRNA and TGF-β mRNA increased（P<0.05）， while the expressions of MMP-1， MMP-2 mRNAs and proteins， MMP-9 mRNA， DCN mRNA and protein decreased（P<0.05） in the anterior vaginal wall. Compared with the acupuncture non-acupoint group， the expression of Col-Ⅲ mRNA and protein and TIMP-1 mRNA increased（P<0.05）， while the expressions of MMP-1， MMP-2 mRNAs and proteins， MMP-9 mRNA， and DCN mRNA and protein decreased （P<0.05）in electroacupuncture group.ConclusionIn SUI rats， it is found that the collagen is lost， the collagen degradation is increased， and the collagen synthesis is decreased in the anterior vaginal wall. Electroacupuncture can inhibit the abnormal degradation of collagen， promote collagen synthesis， increase the total content of collagen in the anterior vaginal wall of SUI rats， and then achieve the purpose of treating SUI， and the curative effect of electroacupuncture at acupoints is better than that of electroacupuncture at non-acupoints.关键词：stress urinary incontinence;electroacupuncture;collagen metabolism;rats model
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发布时间：2022-07-13 - 摘要：ObjectiveTo observe the healing rate of wound surface with external application of Cuyuxunxi Prescription after operation of simulated anal fistula in rats， and to detect the expression of transforming growth factor β1 （TGF-β1）， vascular endothelial growth factor （VEGF） and basic fibroblast factor （bFGF） in wound tissue and serum， and to explore the mechanism of promoting wound healing.MethodsFifty-four SD rats were selected to prepare the model of wounds after simulated anal fistula operation and were randomly divided into model group， control group and experimental group， with 18 rats in each group. After the establishment of the model， 0.9% saline， rivanol and Cuyuxunxi Prescription were respectively given for dressing change once a day for 14 days. The wound healing rate of each group was observed on the 3rd， 7th， and 14th days. The expression levels of TGF-β1， VEGF and bFGF in wound tissue were detected by Western blot （WB）， and the expression changes of serum TGF-β1， VEGF and bFGF were detected by ELISA.Results①Wound healing rate： At three time points， the wound healing rate of the experimental group was better than that of the model group （all P<0.05）， and the rate in the experimental group was better than that in the control group on the 3rd day （P<0.01）. ②WB results： The expression of TGF-β1 in the wound tissue of the experimental group was higher than that of the model group （P<0.01） and the control group （P<0.05） at 3 time points. There was no statistical significance in the expression of VEGF in the wound tissue of each group at 3 time points （P>0.05）. The expression of bFGF in the wound tissue of the experimental group was higher than that of the control group and the model group at 3 time points. The experimental group and the control group were higher than the model group in the expression of bFGF on the 3rd day （P<0.05， P<0.01）， but there was no statistical significance on the 7th and 14th days （P>0.05）. ③ ELISA results： At three time points， the expression of serum TGF-β1 in the experimental group was higher than that in the model group （P<0.05）， and that in the experimental group was higher than that in the control group on the 3rd day（P<0.05）. There was no statistical significance in the expression of VEGF in the serum of each group at 3 time points （P>0.05）. There was no statistical significance in the expression of bFGF in the serum of each group on the 3rd day （P>0.05）， but it was higher in the experimental group on 7th day than that in the model group （P<0.01） and the control group （P<0.05） on the 7th day， and higher in the experimental group on the 14th day than that in the model group （P<0.01） and the control group （P>0.05） on the 14th day.ConclusionsCuyuxunxi Prescription can improve the wound healing rate in the early stage after simulated anal fistula operation in rats. The mechanism of promoting wound healing may be related to the increase of the expression of TGF-β1 and bFGF in wound tissue and serum.关键词：anal fistula;Cuyuxunxi Prescription;rats model;wound healing;traditional Chinese herbal medicine research
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发布时间：2022-07-13 - 摘要：ObjectiveTo observe the effect of CO2 laser moxibustion at Zusanli （ST36） on anxiety-like behaviors and infralimbic cortex （IL） c-Fos expression in a rat model of post-traumatic stress disorder （PTSD）.MethodsTotally 48 healthy male SD rats were randomly divided into control group （n=12）， model group （n=12）， acupoint laser stimulation（LS） group （n=12） and non-acupoint laser stimulation（Sham LS） group （n=12）. The animal model in PTSD， LS and Sham LS groups was established by single-prolonged stress （SPS） method. CO2 laser stimulation was applied to bilateral Zusanli （ST36） in the LS group or non-acupoint in the Sham LS group for 20 minutes， once daily for consecutive 7 days. On the 8th day， rats in each group were tested by open field test （OFT） and elevated plus maze （EPM）， after which the plasma corticosterone levels were measured and the IL c-Fos expression was detected by immunohistochemical techniques， and the correlation analysis of the behavioral changes and the amount of c-Fos expression in the infralimbic cortex of rats in each group was conducted.ResultsRelative to the group and the Sham LS group， LS group was associated with increased center area time spent both in the OFT and EPM， as so as the open arms in the EPM test （P<0.05）. Compared with the group and the Sham LS group， the plasma corticosterone levels were obviously decreased in the LS group （P<0.05）. Compared with the group， the expression of IL c-Fos in the LS group was significantly increased （P < 0.05）， and a significant correlation was detected between the amount of c-Fos expression in IL cortical neurons and behavioral changes in rats （P<0.01）.ConclusionCO2 laser stimulation at Zusanli （ST36） can effectively reduce anxiety-like behaviors in PTSD rats， and its mechanism may be related to the activation of IL cortical neurons.关键词：laser moxibustion;sandwiched moxibustion;ST36;post-traumatic stress disorder;anxiety;infralimbic cortex;rats model
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发布时间：2022-07-13 - 摘要：ObjectiveTo observe the regulation effect of Jianpi Bushen Lishi Recipe on TGF-β1/Smads signal pathway in renal tissue of rats with hyperuricemia nephropathy.MethodsThe rat model of hyperuricemia nephropathy were established， and were randomly divided into model group， allopurinol group and traditional Chinese medicine group（treated with Jianpi Bushen Lishi Recipe）， with 12 rats in each group. Another 12 SD rats were selected as the blank control group. After 8 weeks of administration， ELISA was used to detect the 24 hour urinary protein quantity （24 hUPQ） and the content of serum uric acid（SUA）， serum creatinine（Scr）and blood urea nitrogen（BUN）in plasma. Western blot was used to detect the expressions of transforming growth factor-β1 （TGF-β1）， fibronectin （FN）， collagen Ⅳ （Col4）， Smad homolog 2 （Smad2）， Smad homolog 3 （Smad3）， Smad homolog 7 （Smad7） and phosphorylated Smad2/3/7 proteins in rat renal tissue. The expression levels of TGF-β1， Col4， FN， Smad2/3 and Smad7 mRNA in renal tissue were determined by RT-PCR.ResultsCompared with model group， the expression levels of 24 hUPQ， SUA， Scr and BUN in traditional Chinese medicine group and allopurinol group were significantly decreased（P<0.01），and traditional Chinese medicine group was superior than allopurinol group（P<0.01）. Compared with model group， the expressions of TGF-β1， FN， Col4 protein， TGF-β1 mRNA，Fn mRNA and Col4 mRNA in renal tissue of rats in Chinese medicine group were all decreased significantly （P<0.01）. And the expressions of Smad2/3，p-Smad2/3 protein and Smad2/3 mRNA in renal tissue of rats in traditional Chinese medicine group were decreased significantly （P<0.05）. But the expressions of Smad7 protein and Smad7 mRNA in renal tissue of rats in traditional Chinese medicine group were increased significantly （P<0.05）.ConclusionJianpi Bushen Lishi Recipe may inhibit TGF-β1 signal pathway by regulating the rebalance of positive regulatory protein （Smad2/3） and negative regulatory protein （Smad7） in the TGF-β1 signal pathway，thus delaying renal fibrosis.关键词：hyperuricemia nephropathy;Jianpi Bushen Lishi Recipe;TGF-β1/Smad signal pathway;mechanism
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发布时间：2022-07-13 - 摘要：ObjectiveTo investigate the effect of medicated serum of Liuwei Dihuang Decoction on the proliferation， differentiation and mineralization of H2O2-induced oxidatively damaged MC3T3-E1 cells， and to preliminarily explore its mechanism from the Wnt/β-Catenin， OPG/RANKL pathway， and mitochondrial apoptosis pathway.MethodsThe Liuwei Dihuang Decoction was prepared and given to the adult Wistar rats by intragastric administration， and then the medicated serum of Liuwei Dihuang Decoction was collected. The MC3T3-E1 cells were divided into normal group， model group， N-acetylcysteine （NAC） group， control medicated serum group， low-dose medicated serum group， medium-dose medicated serum group and high-dose medicated serum group. Except for the normal group， the other groups of MC3T3-E1 cells were first pretreated with 1.0 mmol/L H2O2 for 6 h. Then the culture medium was replaced with normal culture medium in the model group， and was replaced with 2.5 mmol/L NAC in the NAC group and replaced by 10% medicated serum with different concentrations in the medicated serum groups．Each group was treated for 24 hours， and then further treatment was given according to the different detection indicators. The proliferation of MC3T3-E1 cells was detected by CCK-8 after incubated for 24 h， 48 h and 72 h respectively. The alkaline phosphatase （ALP） activity was detected 7 days after the induction culture， and the mineralized nodules were detected by Alizarin Red staining 21 days later. RT-PCR was used to detect the expression of key genes such as the mRNA expression of Wnt2， Lrp5， β-Catenin in the Wnt/β-Catenin gene pathway， the mRNA expression of OPG and RANKL in the OPG/RANKL pathway， the mRNA expression of Bcl-2， Caspase-9 and Caspase-3 in the pathway of mitochondrial apoptosis， and the OPG/RANKL ratio was calculated.ResultsCompared with the normal group， the cell proliferation and ALP expression of the model group were decreased （all P<0.01）， and the number of mineralized nodules was decreased. The mRNA expressions of Wnt2， Lrp5 and β-catenin in Wnt/β-catenin gene pathway were decreased （all P<0.01）. In the OPG/RANKL gene pathway， the mRNA expression of OPG was decreased， while the mRNA expression of RANKL was increased， and the ratio of OPG/RANKL was decreased （all P<0.01）. In the mitochondrial apoptosis pathway， the mRNA expression of Bcl-2 was decreased， while the mRNA expressions of Caspase-9 and Caspase-3 were increased （all P<0.01）. Compared with the model group， the medicated serum of Liuwei Dihuang Decoction in each dose group could promote the proliferation of MC3T3-E1 cells （all P<0.01） and the activity of ALP （P<0.05， P<0.01） at 24 h， 48 h， and 72 h， and increase the number of mineralized nodules. In the Wnt/β-Catenin gene pathway， when compared with the model group， except for the low-dose medicated serum group， the mRNA expression of Wnt2 in the medium- and high-dose group was higher than that in the model group （all P<0.01）. The mRNA expression of Lrp5 in each dose group of Liuwei Dihuang Decoction was higher than that of the model group （all P<0.01）， and the mRNA expression of β-Catenin was also higher than that of the model group （P<0.05， P<0.01）. In the OPG/RANKL gene pathway， except for the low-dose medicated serum group， the mRNA expression of OPG in the medium- and high-dose group was higher than that in the model group （P<0.05， P<0.01）， while the expression of RANKL was significantly lower than that in the model group （all P<0.01）， and the ratio of OPG/RANKL in each medicated serum group was higher than that in the model group （all P<0.01） with dose-dependent manner. In the mitochondrial apoptosis pathway， the mRNA expression of Bcl⁃2 in each dose medicated serum group of Liuwei Dihuang Decoction was significantly higher than that in the model group （P<0.05， P<0.01）， while the mRNA expressions of Caspase-9 and Caspase-3 were significantly lower than those in the model group （all P<0.01）.ConclusionThe medicated serum of Liuwei Dihuang Decoction can reduce the damage of MC3T3-E1 caused by H2O2 and generally promote the proliferation， differentiation and mineralization of MC3T3-E1. Its protective effect may be related to the regulation of key targets in the Wnt/β-catenin signaling pathway， OPG/RANKL and mitochondrial apoptosis pathway.关键词：osteoporosis;Liuwei Dihuang Decoction;medicated serum;oxidative stress;mitochondrial apoptosis;traditional Chinese herbal medicine research
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发布时间：2022-07-13 - 摘要：This paper summarized the latest progress in the activation methods of hair follicle stem cells in the process of hair regeneration. New drugs， new transdermal drug delivery methods and new physical stimulation methods can induce the activation of hair follicle stem cells and promote hair growth. In particular， the introduction of nanomaterials， nanotechnology and traditional Chinese medicine compounds， the new transdermal drug delivery system of nanoparticles and microneedles， the biofilm system based on traditional Chinese medicine components， and the physical stimulation methods such as mechanical traction， acupuncture， laser and electro-stimulation have provided new insights for the research on regulating hair growth and the treatment of pathological hair loss.关键词：pathological hair loss;hair follicle stem cell;activation method;transdermal drug delivery;acupuncture;traditional Chinese herbal medicine;physical stimulation;review
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发布时间：2022-07-13
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