最新刊期
期 5 ， 2022
- 摘要：The article summarized Yun’s succession and development experience of integrated traditional Chinese and western medicine from five aspects： the rise of the thought， the formation of Yun’s school， the transmission of Yun’s school and main representatives， Yun’s style of study and Yun’s core theory.关键词：Yun’s school of integrated Chinese and western medicine;Yun Tieqiao;succession;development
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发布时间：2022-07-13 - 摘要：Non-alcoholic fatty liver disease （NAFLD） has become a globally prevalent chronic liver disease， and there is still a lack of targeted therapy. Guided by the classical theory of traditional Chinese medicine （TCM） that “those who has phlegm syndrome should be treated with warm medicine”， our research group creatively applied Linggui Zhugan Decoction （LGZG） to the treatment of NAFLD patients with spleen-yang deficiency syndrome， and found that LGZG can improve fatty acid water solubility and increase fatty acid β-oxidation by working on the the pathway of hepatocyte membrane thyroid hormone receptor. We also discovered and validated the systemic pharmacological mechanism of LGZG in the treatment of NAFLD through multi-omics techniques. Our group confirmed through a multicenter， randomized， double-blind， placebo-controlled clinical trial that LGZG could improve insulin resistance in NAFLD patients with spleen-yang deficiency syndrome， which for the first time provided the evidence-based clinical evidence for the treatment of modern chronic diseases with LGZG. LGZG was included in the first batch of classic formula support projects in the 13th Five-Year Plan of China for the invention of major new drugs. We have completed all the research tasks as required and also the new drug registration of the first classic formula of Linggui Zhugan Decoction. The above-mentioned work is a milestone for promoting the translation and application of traditional Chinese medicine theories and classic formulas in the prevention and treatment of chronic diseases in modern clinics.关键词：non-alcoholic fatty liver disease;metabolic associated fatty liver disease;phlegm syndrome;Linggui Zhugan Decoction;classic formula;translational medicine
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发布时间：2022-07-13 - 摘要：Targeted therapy is one of the most important breakthroughs in the field of clinical oncology in the past 30 years， but there have been challenges in the discovery and development of first-in-class drugs in that hundreds of preclinical drugs crowded to a handful of targets or potential therapeutic target was supposed to be exhausted. On the other hand， 90% of more than 500 cancer drivers identified by oncologists are considered as “yet to be drugged” or “undruggable” proteins. In response to the dilemma， we have put forward a new strategy coupling phenotype-based discovery with target identification and phenotype validation， which is potentially applicable to any target protein. Based on this strategy， traditional Chinese herbal medicine （TCHM） has unique advantages in the discovery of first-in-class drugs as it has good biological activity， rich resource and long-term clinical application experience， and TCHM also offers valuable phenotypes that are particularly attractive for phenotype-based discovery of agents targeting “yet to be drugged” or “undruggable” proteins. Correspondingly， large-scale target identification allows us to fully dissect the underlying mechanism-of-action for the clinical phenotypes of TCHM， which also provides an opportunity for the modernization of TCHM.关键词：traditional Chinese herbal medicine;targeted therapy;phenotype;target identification;first-in-class drug discovery;“undruggable” protein
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发布时间：2022-07-13 - 摘要：This paper introduced professor He Liqun’s experience in treating chronic kidney disease（CKD） with the method of “clearing dampness and dissipating blood stasis”. Professor He believed that the etiology and pathogenesis of the disease was mainly caused by the spleen-kidney deficiency and related to damp-heat and blood stasis. He proposed the method of “clearing dampness and dissipating blood stasis” for the treatment of CKD. Clinical treatments were attached great importance to clearing heat and eliminating dampness and improving the occasion； activating blood circulation to dissipate blood stasis and avoiding consume healthy qi； clearing dampness and dissipating blood stasis and flexible adaptation .And one proven case was presented．关键词：chronic kidney disease;chronic renal failure;etiology and pathogenesis;clearing dampness and dissipating blood stasis;famous doctors’ experience;traditional Chinese medicine therapy
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发布时间：2022-07-13 - 摘要：The purpose of this paper is to introduce the experience of Professor Fan Guanjie in the treatment of hyperthyroidism by using the “Fan’s eight development-regularity sequential method”. It is believed that the treatment of hyperthyroidism should identify the core pathogenesis from the key symptoms， and then treat the disease based on syndrome differentiation. The core pathogenesis are qi stagnation， effulgent fire， phlegm congelation， blood stasis， deficiency of both qi and yin， liver yin deficiency， kidney deficiency and spleen deficiency， which are treated by the methods of soothing the liver and regulating qi， clearing heat and purging fire， resolving phlegm and resolving dampness， activating blood and resolving stasis， replenishing qi and enriching yin， enriching liver yin， tonifying the kidney and securing the root ，and fortifying the spleen respectively. The prescriptions mainly include a series of Chinese medicines and are modified focused on the law of disease development， the sequence of treatment and the importance of the liver. And one proven case is presented.关键词：hyperthyroidism;Fan’s eight development-regularity sequential method;liver depression;series of Chinese medicines;traditional Chinese medicine therapy
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发布时间：2022-07-13 - 摘要：Through one case of refractory hyperthyroidism treated by Professor Li Hong， this paper briefly analyzes the choice of iodine-containing traditional Chinese herbal medicines. It is considered that refractory hyperthyroidism is mostly caused by chronic consumption of qi and yin， devitalized yang qi， yin blood insufficiency， yin deficiency with exuberant heat and internal production of deficiency fire， and botanical drugs with less iodine （such as Cyperi Rhizoma， PrunellaE Spica， etc.） can also make the patient's condition repeated and difficult to control. Therefore， the treatment principle of replenishing qi and enriching yin is put forward from the point of view of traditional Chinese herbal medicines containing no iodine， and traditional Chinese herbal medicines such as Pseudostellariae Radix， Glehniae Radix， Ophiopogonis Radix， Anemarrhenae Rhizoma are often selected in the treatment of refractory hyperthyroidism.关键词：refractory hyperthyroidism;iodine containing Chinese herbal medicine;replenishing qi and enriching yin;traditional Chinese medicine therapy
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发布时间：2022-07-13 - 摘要：This paper introduces the understanding and clinical application of Wumei Wan by the representative successors of Longsha Medical School. Huang Huang believes that Wumei Wan mainly treats diseases characterized by vomiting， restlessness， cold limbs， pain， and chronic dysentery. Gu Zhishan believes that Wumei Wan mainly treats patients with diseases due to dysfunction of Jueyin Pivot， disconnection of yin qi and yang qi， cold and heat complex， and some of the diseases appeared or aggravated in the second half of the night. And three proven medical cases are presented.关键词：Wumei Wan;Longsha Medical School;Huang Huang;Gu Zhishan;classical prescriptions;time of six meridian diseases to be resolved
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发布时间：2022-07-13 - 摘要：The prescription of Baidu Powder and the application of wind herbs by the Yishui school in treating the spleen and stomach diseases were comprehensively analyzed. It was believed that the method of sailing boat against stream was consistent with the application of wind herbs by the Yishui school. The essence was using wind herbs to treat the spleen and stomach diseases， because wind herbs had functions of promoting yang， dissipating dampness， eliminating dampness， soothing the liver to promote the spleen and stomach for stopping diarrhea.关键词：method of sailing boat against stream;Ren Shen Baidu Powder;wind herbs;spleen and stomach diseases
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发布时间：2022-07-13 - 摘要：To explore the effect of intestinal-kidney axis on chronic kidney disease based on the theory of hidden pathogen. The onset of chronic kidney disease was occult， which was similar to the hidden pathogen in traditional Chinese medicine. The damage of kidney caused by endotoxin and micro-inflammation on account of intestinal flora disturbance was similar to that caused by hidden pathogens. When the immune inflammation of intestinal mucosa occurred， it could lead to the aggravation of chronic kidney disease， which was similar to the external occurrence of hidden pathogen and hidden pathogen transforming into fire.关键词：hidden pathogen;intestine-kidney axis;intestinal flora;chronic kidney disease
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发布时间：2022-07-13 - 摘要：ObjectiveTo analyze the comorbidity mechanism of non-alcoholic fatty liver disease （NAFLD） and hypertension and the distribution characteristics of TCM constitution types among the elderly in Shanghai communities for the purpose of providing theoretical basis for TCM constitution adjustment of the elderly.MethodsAccording to the prevalence of NAFLD and hypertension， elderly people who underwent routine physical examination in Beicai Community Health Service Center of Pudong New Area， Shanghai were divided into four groups： normal group， hypertension group， NAFLD group， and NAFLD combined with hypertension group （co-morbid group）. The baseline data and TCM constitutions questionnaire were retrospectively analyzed. We compared general data such as gender， waist-hip ratio， body mass index （BMI）， and laboratory indexes such as triglyceride （TG）， total cholesterol （TC）， high-density lipoproteins cholesterol （HDL-C）， alanine aminotransferase （ALT）， aspartate aminotransferase （AST）， uric acid （UA）， and investigated the distribution characteristics of TCM constitutions. Logistic regression was used to estimate risk factors of NAFLD and hypertension and correlation analysis was performed for some factors.Results①A total of 550 participants （170 cases in the co-morbid group， 179 cases in the NAFLD group， 127 cases in the hypertension group and 74 cases in the normal group） were included. ②Compared with the condition in the normal group， BMI， waist-hip ratio， systolic blood pressure， diastolic blood pressure， fasting blood glucose （FBG）， TG， ALT， AST， UA levels increased （P<0.01） and HDL-C level decreased （P<0.01） in the co-morbid group； the waist-hip ratio， BMI and ALT， AST， UA levels increased （P<0.01） and HDL-C level decreased （P<0.05） in the NAFLD group； systolic and diastolic blood pressure and ALT and AST levels increased （P<0.05， P<0.01） in the hypertensive group. Compared with the condition in the hypertension group， the waist-hip ratio， BMI and FBG， TG， ALT， AST and UA levels increased （P<0.05， P<0.01） and HDL-C level decreased （P<0.01） in the co-morbid group； the waist-hip ratio， BMI， systolic and diastolic blood pressure and TG， ALT， UA levels increased （P<0.05， P<0.01） and HDL-C level decreased （P<0.01） in the NAFLD group. Compared with the condition in the NAFLD group， BMI， waist-to-hip ratio， systolic blood pressure， diastolic blood pressure and UA level increased （P<0.05， P<0.01） and HDL-C level decreased （P<0.05） in the co-morbid group. ③In the co-morbid group， the dominant body constitutions were phlegm-dampness constitution （11.18%）， complex constitution （11.18%） and other biased constitutions， and the difference in the overall distribution of TCM constitutions was statistically significant compared with that of the NAFLD group and hypertension group （P<0.05）. ④Logistic regression analysis showed that obesity ［OR=9.883， 95% CI（3.347-29.180）］， high TG ［OR=4.306， 95% CI（1.762-10.522）］， low HDL-C ［OR=4.796， 95% CI（1.034-22.242）］， and high UA ［OR=3.255， 95% CI（1.229-8.625）］ were risk factors for the co-morbid condition of NAFLD and hypertension， with obesity as the greatest risk factor after adjusting gender， history of diabetes， hyperlipidemia， cardiovascular diseases， and osteoporosis. ⑤According to the correlation analysis， there was a linear positive correlation between BMI and TG in the co-morbid group （r=0.306， P<0.01）.ConclusionThe comorbidity mechanism of NAFLD and hypertension may be related to elevated BMI， TG and UA levels and reduced HDL-C level， and phlegm-dampness constitution and complex constitution are dominant TCM constitution types. The identification of phlegm-dampness and complex constitutions should be the key emphasis of TCM constitution identification when physical examination is conducted in communities so that the biased constitutions can be improved or regulated as early as possible to effectively prevent and treat the comorbid condition of NAFLD and hypertension among the elderly in communities.关键词：non-alcoholic fatty liver disease;hypertension;elderly people;traditional Chinese medicine constitution
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发布时间：2022-07-13 - 摘要：ObjectiveTo investigate the clinical efficacy of traditional Chinese medicine （TCM） medicated diet Huangqi Zhuti Decoction （Astragalus pork trotter soup） on severe diabetic foot gangrene complicated with severe hypoproteinemia.MethodsEighty-seven patients with severe diabetic foot gangrene complicated with severe hypoproteinemia were randomly divided into a treatment group （n=44） and a control group （n=43）. Both groups were given the conventional treatment and a diabetes diet. The treatment group was additionally administered with Huangqi Zhuti Decoction， while the control group was given compound amino acid capsules. The treatment lasted 8 weeks. The clinical efficacy and wound healing were observed， and the changes of serum nutritional indexes， the levels of major and minor elements， peri-wound skin temperature， transcutaneous oxygen tension （TcPO2）， nerve conduction velocity （CV） and amplitude （Amp） were compared， and the safety evaluation was conducted.Results①During the trial， there were 2 drop-out cases in the treatment group and 1 drop-out case in the control group. Finally， 84 cases completed the experiment， with 42 cases in each group. ②The total effective rate of the treatment group was 90.48% and that of the control group was 71.43%. The treatment group had better clinical efficacy than the control group （P<0.05）. ③According to the intra-group comparison before and after treatment， the wound area of the two groups decreased after treatment （P<0.05）； After treatment， the wound area of the treatment group was smaller than that of the control group （P<0.05）. ④With the prolongation of treatment time， the serum levels of nutritional indexes （albumin， prealbumin and hemoglobin） of the two groups were improved in different degrees （P<0.01， P<0.05）， and the treatment group had better improvement than the control group （P<0.01， P<0.05）. ⑤During the trail， the contents of Ca， Mg， Zn and Fe in serum of control group had no significant change （P>0.05）. In the treatment group， compared with those before treatment， the contents of Mg and Fe in serum increased after 4 weeks of treatment （P<0.05） and the contents of Mg， Zn and Fe in serum increased significantly after 8 weeks of treatment （P<0.01）； the serum contents of Mg， Zn and Fe in the treatment group after 8 weeks of treatment were higher than those after 4 weeks of treatment （P<0.01）. There was no significant difference in serum contents of Ca， Mg， Zn and Fe between the two groups after 4 weeks of treatment （P>0.05）； After 8 weeks of treatment， the serum contents of Mg， Zn and Fe in the treatment group were higher than those in the control group （P<0.01）. ⑥During the trial， there was no significant change in peri-wound skin temperature and transcutaneous oxygen tension in both groups （P>0.05）； According to the inter-group comparisons at each observation point-in-time， there was no significant difference in peri-wound skin temperature and transcutaneous oxygen tension between the two groups （P>0.05）. ⑦During the trail， the values of CV and Amp in the control group did not change significantly （P>0.05）； With the prolongation of treatment time， the values of CV and Amp in the treatment group increased gradually （P<0.01， P<0.05）. After 8 weeks of treatment， the values of CV and Amp in the treatment group were higher than those in the control group （P<0.01）. ⑧There was no obvious adverse reaction in cases of the two groups.ConclusionTCM medicated diet Huangqi Zhuti Decoction has achieved satisfactory therapeutic effect on severe diabetic foot gangrene complicated with severe hypoproteinemia， which can significantly improve the nutritional status and neurological function of patients， increase the levels of major and minor elements， and promote wound healing without significant adverse reactions.关键词：diabetic foot;hypoproteinemia;traditional Chinese medicine medicated diet;Huangqi Zhuti Decoction;supportive tonification theory;clinical trial
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发布时间：2022-07-13 - 摘要：ObjectiveTo observe the effect of Baduanjin on glucose and lipid metabolism and quality of life of pre-diabetic patients in the community.MethodsEighty patients with pre-diabetes were randomly divided into a treatment group （n=40） and a control group （n=40）. The control group was administered with routine lifestyle intervention and walking exercise， while the treatment group was administered with routine lifestyle intervention and Baduanjin exercise. The intervention lasted 24 weeks. The changes of body weight， body mass index （BMI）， waist-hip ratio （WHR）， fasting blood glucose （FPG）， 2-hour postprandial blood glucose （2hPG）， glycosylated hemoglobin （HbA1c）， fasting insulin （FINS）， 2-hour postprandial insulin， insulin resistance index （HOMA-IR）， total cholesterol （TC）， triglyceride （TG）， low density lipoprotein cholesterol （LDL-C）， high density lipoprotein cholesterol （HDL-C） and the MOS item short from health survey （SF-36） scores in both groups were observed and compared.Results①Finally 76 cases completed the trial， with 39 cases in the treatment group and 37 cases in the control group.②After intervention， the body weight and BMI of the two groups decreased significantly （P<0.05）. In the treatment group， the difference in WHR of patients before and after intervention was significant （P<0.05）， while that was not significant in the control group （P>0.05）. After intervention， the body weight and BMI of patients in the treatment group were significantly lower than those in the control group （P<0.05）. ③After intervention， the levels of FPG， 2hPG， HbA1c， FINS， 2h postprandial insulin and HOMA-IR in the two groups were significantly lower than those before intervention （P<0.05）； and the improvement of blood glucose related indexes in the treatment group was significantly better than that in the control group （P<0.05）. ④After intervention， the levels of TC， TG and LDL-C in the two groups were significantly lower than those before intervention （P<0.05）； and the improvement of blood lipid related indexes in the treatment group was significantly better than that in the control group （P<0.05）. ⑤After intervention， the physical health and mental health scores of SF-36 in both groups were significantly higher than those before intervention （P<0.05）； and the physical health and mental health scores in the treatment group were significantly higher than those in the control group （P<0.05）.ConclusionBaduanjin exercise can effectively reduce body mass index and waist-hip ratio， regulate glucose and lipid metabolism， improve insulin resistance and the quality of life of pre-diabetic patients in the community.关键词：pre-diabetes;Baduanjin;preventive treatment of disease;non-pharmacologic therapy;quality of life
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发布时间：2022-07-13 - 摘要：ObjectiveTo study the effect of capsaicin on the improvement of liver and intestinal mucosal lesions in mice with alcoholic liver disease （ALD）， and explore its related mechanisms.MethodsTwenty-four male C57/BL6 mice were randomly divided into the control group， the alcohol group and the alcohol capsaicin group. The mice in the alcohol group and the alcohol capsaicin group were fed with ethanol liquid diet for 4 weeks and gavaged with alcohol to replicate the alcoholic liver disease model. The control group was administered with isocaloric liquid feed. From the 3rd week of modeling， the alcohol capsaicin group was given capsaicin （10 mg/kg body weight） by gavage every other day and three times a week， while the other two groups were given an equal volume of distilled water by gavage. After 4 weeks of modeling， mice in the alcohol group and the alcohol capsaicin group were given 31.5% ethanol by gavage， while the mice in the control group were given 45% dextrin by gavage. The mice were sacrificed 9 hours later， and blood， liver and small intestine tissue samples were collected. Serum alanine aminotransferase （ALT）， aspartate aminotransferase （AST） and triglyceride （TG） levels were detected by enzyme-linked immunosorbent assay. The pathological changes of liver and small intestine mucosa were observed after HE staining. The lipid deposition in the liver was observed by oil red O staining. RT-PCR was used to detect the mRNA expression levels of liver inflammatory factors including tumor necrosis factor-α （TNF-α）， interleukin 6 （IL-6）， interleukin 1β （IL-1β）， small intestinal tissue inflammatory factors including TNF-α， IL-6， monocyte chemoattractant protein 1 （MCP-1） and tight junction proteins including ZO-1， occludin and claudin-1.ResultsCompared with the control group， the serum ALT， AST， TG levels and liver mRNA expressions of TNF-α， IL-6， IL-1β were significantly increased in the alcohol group （P<0.05）. HE staining showed that hepatic steatosis and inflammatory cells in the alcohol group was obvious. Oil red staining showed a large number of lipid droplets in the liver cells of the alcohol group. Shortened small intestine villi， epithelial cell edema and increased submucosal inflammatory cell infiltration in the alcohol group were observed by HE staining of small intestine mucosa. Compared with those in the control group， mRNA relative expression of TNF-α， IL-6 and MCP-1 in the small intestine tissue of mice were increased significantly （P<0.05）， while mRNA relative expression of tight junction protein ZO-1， occludin and claudin-1 were significantly decreased （P<0.01）. Compared with those in the alcohol group， the serum ALT， AST， TG levels and liver mRNA expressions of TNF-α， IL-6， IL-1β in the alcohol capsaicin group were significantly reduced （P<0.05）， hepatic steatosis and intestinal mucosal pathological damage were reduced， mRNA expressions of intestinal mucosal inflammatory factors such as TNF-α， IL-6 and MCP-1 in the alcohol capsaicin group mice were significantly down-regulated （P<0.05）， while mRNA expressions of tight junction proteins including ZO-1， occludin， and claudin-1 were significantly up-regulated （P<0.01）.ConclusionCapsaicin has a protective effect on the liver of mice with alcoholic liver disease， and its mechanism of action may be related to the intestinal mucosal barrier protection and intestinal leakage reduction.关键词：capsaicin;alcoholic liver disease;intestinal mucosal barrier;model mice;traditional Chinese herbal medicine research
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发布时间：2022-07-13 - 摘要：Objactive To explore the common mechanism of aristolochic acid I （AAⅠ） inducing liver and kidney injury in mice based on transcriptomics.MethodsMale C57BL/6 mice were randomly divided into the normal group （n=6） and the model group （n=9）. The mice in the model group AAⅠ was injected intraperitoneally with a dose of 20 mg/kg once a day for 5 d and then executed. The contents of SCr， BUN， ALT and AST in serum were detected， and histological and pathological characteristics of liver and kidney were analyzed in two groups of mice. RNA was extracted from three liver and kidney tissues of the normal group and the model group respectively， and sequenced by high-throughput transcriptome. Differential genes were defined as differentially expressed genes （Fold Change） ≥2.0 and P<0.01. The differential genes in normal liver， kidney and model liver and kidney were screened respectively， and the intersection of differential genes in the liver and kidney was analyzed by gene ontology（GO）and Kyoto encyclopedia of genes and genomes（KEGG）database. The differential genes with the most related genes were identified by protein interaction network analysis and verified by qRT-PCR.ResultsCompared with the normal group， the activities of ALT and AST in serum and the contents of SCr and BUN in the model group were significantly increased. The structural disorders of hepatic lobule， focal necrosis and mild inflammation in the portal area were observed in the liver tissue， and glomerular edema， glomerular pyknosis and renal tubular epithelial cell necrosis and exfoliation were observed in the renal tissue. Transcriptome sequencing showed that there were 298 common differential genes in the liver and kidney. GO and KEGG analysis of common differential genes in the liver and kidney showed that GO classification related to small molecular catabolism， organic acid catabolism， carboxylic acid catabolism， oxidoreductase activity， antioxidant activity and glutathione peroxidase activity was significantly enriched， and carbon metabolism， insulin resistance， complement and coagulation cascade， NF-κb， JAK-STAT and PPAR signaling pathways were significantly enriched in KEGG pathway. Protein interaction network analysis and qRT-PCR verification showed that there were significant differences in the expressions of Stat3， Cdh1， Myc， Ugt2b38 and Egr1， which were consistent with the results of transcriptome.ConclusionsTranscriptome reveals that the common mechanism of hepatorenal toxicity induced by AAⅠ is mainly related to complement and coagulation cascade， redox process， NF-κb， JAK-STAT signal pathway and so on. The hepatorenal toxicity mechanism of AAⅠ should be further explored based on Stat3， Cdh1， Myc and other genes.关键词：aristolochic acid;transcriptomics;hepatorenal toxicity;mice;traditional Chinese herbal medicine research
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发布时间：2022-07-13 - 摘要：ObjectiveTo observe the effect of Qinghua Granules on intestinal type 1 taste receptor and its downstream signal factors in db/db diabetic mice.MethodsThe db/db mice were divided into the model group， the positive control group and Qinghua Granules group. The mice were given respectively the same amount of 0.9%NaCl solution， fructose and Qinghua Granules daily for 4 weeks. The concentrations of serum glucagon-like peptide-1 （GLP-1） and glycosylated hemoglobin （HbA1c） were analyzed by ELISA， and the expressions of GLP-1， type 1 taste receptor （TAS1R1， TAS1R2， TAS1R3） and its downstream signal factors α-gustducin， phospholipase Cβ2 （PLCβ2） and transient receptor potential cation channel protein 5 （TRPM5） mRNA and protein in ileal tissue of mice were detected by qRT-PCR and Western blot respectively. The level of GLP-1 protein in jejunal tissue was detected by immunohistochemical technique， and the changes of fasting blood glucose and body mass were observed at the same time.ResultsCompared with the model group， the Qinghua Granules group could increase the serum concentration of GLP-1， GLP-1 mRNA and protein in ileal tissue， up-regulate the expression of TAS1R2 and its downstream signal factors including PLCβ2， TRPM5 mRNA and protein in mouse ileum （P<0.05）， and up-regulate the levels of TAS1R1 and downstream signal factor α⁃gustducin mRNA and protein in ileal tissue of mice （P<0.05）. In terms of the peripheral effect of GLP-1， compared with the model group， the fasting blood glucose， the increase of body mass and the level of HbA1c in the Qinghua Granules group were significantly lower than those in the model group （P<0.05）.ConclusionQinghua Granules can promote intestinal L cells to synthesize and secrete GLP-1 by activating type 1 taste receptor pathway， thus exerting the peripheral effect of GLP-1 in regulating blood glucose homeostasis and reducing body mass.关键词：Qinghua Granules;diabetes mellitus type 2;type1 taste receptor;glucagon-like peptide-1;db/db mice;traditional Chinese herbal medicine research
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发布时间：2022-07-13 - 摘要：ObjectiveTo study the effect of Buyang Huanwu Decoction on the endothelialization of coronary stents and the mechanism of regulating the function of endothelial progenitor cells （EPCs）.MethodsTwelve male minipigs were divided into two groups： the treatment group （Buyang Huanwu Decoction + routine Western medicine treatment） and the control group （routine Western medicine treatment）. Each minipig was implanted with a firehawk stent of 3.0 mm×18.0 mm to establish the stent implantation model of porcine coronary artery balloon injury. Six minipigs in each group were intervened for 30 days. Through the experimental study on the stent implantation model of porcine coronary artery balloon injury， the effect of Buyang Huanwu Decoction on the endothelialization of porcine coronary stent was observed. Optical coherence tomography （OCT） was used to evaluate the endothelialization of stents. Flow cytometry was used to study the effect of Buyang Huanwu Decoction on the number of EPCs in peripheral circulation， and the effect of Buyang Huanwu Decoction on the expression of vascular endothelial growth factor （VEGF） and basic fibroblast growth factor （bFGF） in EPCs was studied.Results① The results of animal experiment showed that compared with the control group， the treatment group could promote the endothelialization of the stent， the endothelial coverage rate of the stent surface was higher， and the number of peripheral circulating EPCs increased significantly on the 3rd， 5th and 7th day after operation， and the difference was statistically significant （P<0.001）. ② The results of EPCs culture in vitro showed that the expressions of VEGF and bFGF in the treatment group were higher than those in the control group on the 7th day after operation （P<0.05）.ConclusionBuyang Huanwu Decoction can increase the number of EPCs in peripheral circulation and up-regulate the expression of VEGF and bFGF in porcine coronary artery balloon injury model， which is expected to play a key role in promoting stent endothelialization.关键词：Buyang Huanwu Decoction;coronary heart disease;minipig;endothelial progenitor cells;stent endothelialization;traditional Chinese herbal medicine research
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发布时间：2022-07-13 - 摘要：ObjectiveTo explore the promoting effect of Ruanjian Qingmai Grain （RJQM） on the growth of Flila-EGFP transgenic zebrafish （Hereinafter referred to as zebrafish） vessels in different modes of administration in order to provide experimental data support for the clinical application of RJQM.MethodsThe vascular injury model of zebrafish was constructed by VEGFR tyrosine kinase inhibitor（VRI），and was randomly divided into RJQM low dosage （0.025 g/L）， RJQM medium dosage （0.1 g/L）， RJQM high dosage （0.4 g/L） treated groups. The promoting effect of different concentrations of RJQM in angiogenesis of zebrafish was studied through drug treatment， early intervention and pre-prevention.ResultsIn the drug treatment mode， compared with the model group， the high dose of RJQM significantly promoted the growth of intersegmental blood vessels （ISVs） in zebrafish with statistical significance （P<0.001）. In the early intervention mode， compared with the model group， the low， medium and high doses of RJQM all significantly promoted the growth of ISVs in zebrafish with statistical significance （P<0.01，P<0.001）； In the pre-prevention mode， compared with the model group， the low， medium and high doses of RJQM promoted the growth of ISVs in zebrafish， but there was no significance difference （P>0.05）.ConclusionRJQM has a significant effect of promoting angiogenesis of zebrafish in the administration modes of drug treatment and early intervention.关键词：RuanJian Qingmai Grain;arteriosclerosis obliterans;angiogenesis;zebrafish;traditional Chinese herbal medicine research
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发布时间：2022-07-13 - 摘要：ObjectiveTo establish a method for qualitative and quantitative analysis of zeaxanthin dipalmitate （ZD） in fruits of Lycii Fructus.MethodsA qualitative identification method for ZD was established by thin layer chromatography （TLC） with petroleum ether： ethyl acetate at 9.5∶0.5 （v/v） as developing solvent； the temperature was set at room temperature 25 ℃， and the influence of humidity was ignored. The thin layer plates were examined under sunlight and ultraviolet light at 365 nm. ZD content was determined by ultra-performance convergence chromatography/photodiode array detector （UPC2-PDA） equipped with a HSS C18 column （150 mm×3.0 mm， 1.8 μm）， and CO2 and cosolvent methanol were used as mobile phase for gradient elution at a flow rate of 0.8 mL/min.ResultsThe established TLC method can be used for rapid and simple identification of ZD in Lycii Fructus of different origins with clear spots and good repeatability. UPC2-PDA method achieved green and efficient content determination of this compound. The linear relationship of ZD was good in the range of 12.5 μg/mL~500 μg/mL， R2 was 0.999 7， the average recovery was 92.4%， and RSD was 1.15%. In 26 batches of Lycii Fructus samples from different origins， the content of ZD was ranged from 0.929 mg/g to 3.21 mg/g， and there was no significant difference in the ZD content of Lycii Fructus from different origins.ConclusionThe established qualitative and quantitative methods are specific and repeatable that can be used for quality control of Lycii Fructus and provide a scientific basis for the further study on the improvement of quality standard of Lycii Fructus.关键词：Lycii Fructus;zeaxanthin dipalmitate;TLC;UPC2-PDA;traditional Chinese herbal medicine research
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发布时间：2022-07-13 - 摘要：Chronic hepatitis B （CHB） is a major public health problem that threatens human health. Inadequate immune response is an important cause of persistent hepatitis B virus （HBV） infection， aggravated liver damage and prolonged course of disease. The immunomodulatory effects of traditional Chinese medicine （TCM） on CHB were reviewed from the perspectives of innate immunity and adaptive immunity. TCM can regulate the immune status of CHB patients by regulating macrophage phagocytosis and dendritic cell （DC） immune function， increasing natural killer cell （NK） activity， improving the imbalance of helper T subsets and enhancing the immune response capacity of T cell subsets.关键词：chronic hepatitis B;traditional Chinese medicine;immunomodulatory;innate immunity;adaptive immunity;review
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发布时间：2022-07-13 - 摘要：This paper reviewed the research progress on Chinese medicine in the prevention and treatment of diabetic nephropathy （DN）by interfering with nuclear factor-kappa B （NF-κB） related signaling pathways. Studies had been confirmed that DN was accompanied with abnormal activation of NF-κB related signaling pathways. Chinese medicine monomer （calycosin， berberine， tripterine， etc.） and Chinese medicine compound prescriptions（Qidan Dihuang Decoction， Huangqi Decoction， Yishen Capsule， etc.） could effectively prevent DN by interfering with NF-κB related signaling pathways.关键词：NF-κB signaling pathway;diabetic nephropathy;traditional Chinese herbal medicine;review
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发布时间：2022-07-13
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