最新刊期
期 10 ， 2022
- 摘要：ObjectiveTo observe the effect of regular practice of Kangyi Qiangshen Gong （anti-epidemic and body-strengthening exercise） on the 7-day nucleic acid negative conversion rate and anxiety in patients with mild and asymptomatic infection of the COVID-19 Omicron variant.MethodsA total of 580 patients with mild and asymptomatic infection of COVID-19 Omicron variant were randomly divided into a treatment group （n=290） and a control group （n=290）. The control group was administered with Jingyin Granules as the basic treatment and daily nucleic acid test， while the treatment group was guided to practice Kangyi Qiangshen Gong regularly besides all the intervention measures in the control group. The interventions in both groups lasted 7 days. Nucleic acid negative conversion rate， hospitalization duration and self-rating anxiety scale （SAS） scores were observed and compared.Results①During the trial， there were no drop-outs in both groups， and all cases completed the trial. ②There was statistically significant difference in the 7-day nucleic acid negative conversion rate between the two groups， and the treatment group had significantly higher rate than the control group （P<0.05）. ③There was no significant difference in the average length of stay in hospitals between the two groups （P>0.05）. ④After intervention， there was significant difference in the positive rate of anxiety between the two groups （P<0.05）. ⑤There were significant differences in SAS scores between the two groups before and after intervention （P<0.05）； The SAS score of the treatment group was significantly lower than that of the control group after intervention （P<0.05）.ConclusionRegular practice of Kangyi Qiangshen Gong can increase the nucleic acid negative conversion rate within 7 days in mildly infected and asymptomatic patients with the COVID-19 Omicron variant infection and relieve their anxiety.关键词：COVID-19;SARS-CoV-2;Omicront variant;traditional Chinese medicine exercise;nucleic acid negative conversion;anxiety;clinical trial
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发布时间：2022-10-26 - 摘要：Since the outbreak of novel coronavirus disease （COVID-19）， traditional Chinese medicine （TCM） has been playing a unique role in the prevention and treatment of COVID-19. Meanwhile， the COVID-19 pandemic has brought opportunities and significant challenges to the TCM clinical research. By reviewing and analyzing the problems in the practice and management of TCM clinical research on COVID-19 during the normalization stage of pandemic prevention and control， we aim to explore and discuss the topic selection and project initiation， protocol design， ethical review， research implementation， and research management of TCM clinical research in this article， with a view to providing some suggestions for the scientific and standardized conduct of TCM clinical research on COVID-19 during the pandemic.关键词：COVID-19;SARS-CoV-2;Omicron variant;traditional Chinese medicine therapy;clinical research;pandemic prevention and control
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发布时间：2022-10-26 - 摘要：ObjectiveTo analyze the traditional Chinese medicine （TCM） syndrome characteristics in Omicron variant infected patients with chronic kidney disease （CKD） in Shanghai.MethodsCases of Omicron variant infected CKD patients who were hospitalized in Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine were included. The baseline data such as gender， age， underlying diseases and CKD stages， and TCM symptom information such as cough， fever， fatigue， anorexia， sore throat， dry mouth， bitter taste， loose stool and constipation， as well as tongue and pulse condition were collected to analyze TCM syndrome characteristics. The patients’ lung CT images were reviewed through the medical record system to determine the severity of COVID-19 from the perspective of western medicine and the TCM syndrome differentiation was further conducted in patients with different severity of COVID-19.Results①A total of 367 hospitalized cases with Omicron infection were screened， and 53 cases with CKD were included for research. Among 53 cases， patients were classified as CKD stage G3a， G2， G1 or G5， and most of them were the elderly over 80 years old. There were more female patients than male ones， and one of the most common underlying diseases was hypertension. ②The common clinical symptoms were cough （62.3%）， anorexia （47.2%）， fatigue （45.3%）， bitter taste （37.7%） and fever （34.0%）. ③In terms of tongue condition， 60.4% of the patients had fat tongue body， 62.3% had red tongue color， 43.4% had thin tongue coating and 66.0% had yellow tongue coating； In terms of pulse condition， 56.6% of the patients had slippery pulse and 52.8% had fine pulse. ④Among 53 patients， there were 21 mild cases， 29 moderate cases， 2 severe cases and 1 critical case. ⑤The most common TCM syndromes of mild and moderate cases were damp-heat accumulating in the lungs syndrome （34.0%） and damp-toxin stagnating in the lungs syndrome （32.1%）； There was one severe case identified as flaring heat in qifen and yingfen syndrome （1.9%） and the other as pestilential toxicity blocking the lungs syndrome （1.9%）； The critical case met the syndrome differentiation criteria of inner blocking causing collapse syndrome （1.9%）.ConclusionsIn our research， the majority of patients with COVID-19 Omicron variant infection combined with chronic kidney disease are the elderly with severe underlying diseases， and their clinical manifestations are mainly respiratory， together with systemic symptoms. Mild cases mainly have damp-heat accumulating in the lungs syndrome， moderate cases mainly have damp-toxin stagnating in the lungs syndrome， severe cases can be identified as pestilential toxicity blocking the lungs syndrome and flaring heat in qifen and yingfen syndrome， and critical cases can be identified as inner blocking causing collapse syndrome.关键词：COVID-19;SARS-CoV-2;Omicron variant;chronic kidney disease;traditional Chinese medicine syndrome
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发布时间：2022-10-26 - 关键词：influenza;seasonal;influenza virus;traditional Chinese medicine therapy;prevention;expert consensus
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发布时间：2022-10-26 - 摘要：Professor Cai Gan’s experience in treating irritable bowel syndrome （IBS） based on the “three concepts” theory is introduced. It is considered that IBS is caused by a combination of multiple factors and gastrointestinal malfunction. Therefore， Pro. Cai emphasizes the theory of “three concepts” （i.e.， holistic concept， dynamic concept， and balanced concept） as the basis for treatment， regulating zang-organs for fu-organs related disorders， changing treatment methods with syndromes， adjusting formulas flexibly and prescribing herbal medicines mild in nature. Remarkable therapeutic effects have been achieved with two proven cases attached.关键词：irritable bowel syndrome;diarrhea;constipation;traditional Chinese medicine therapy;famous doctor’s experience;Changjitai;visceral hypersensitivity
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发布时间：2022-10-26 - 摘要：In the clinical treatment of febrile diseases and miscellaneous diseases， the foundation was laid for the clinical prescription and medication of traditional Chinese medicine （TCM）， and there are innovations in the methods of prescription and medication in ancient and modern times. The differences in depth and breadth in the understanding of diseases， syndromes and symptoms between ancient and modern times also influence the prescription and medication. Therefore， Chinese medicine should not only inherit the tradition， but also innovate incessantly when encountering various modern clinical situations.关键词：prescription and medication;method;innovation;ancient and modern integration
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发布时间：2022-10-26 - 摘要：ObjectiveTo investigate the correlation between overweight/obesity and traditional Chinese medicine （TCM） constitution， to find TCM constitution factors that may affect overweight/obesity， and to provide a basis for clinical prevention and control of overweight/obesity based on a cross-sectional survey study.MethodsThe clients of the physical examination center of Yueyang Hospital of Integrative Traditional Chinese and Western Medicine affiliated to Shanghai University of Traditional Chinese Medicine were selected as the subjects of the survey， and data on general demographic characteristics such as gender， age， marital status， and place of origin were collected. Digital ultrasonic electronic body scales were used to measure height and body mass， and body mass index （BMI） was calculated. The type of TCM constitution was evaluated with the TCM constitution identification software. Logistic regression model was used to analyze the correlation between overweight/obesity and general demographic data and TCM constitution.Results①Totally 761 subjects were included， among which 303 cases （BMI≥24） were categorized as overweight/obesity group， and 458 cases （18.5≤BMI<24） were categorized as normal weight group. There was significant difference in gender distribution between the two groups （P<0.05）. ②Participants in both groups often possessed two or more constitutional types， and seldom had just one type. Compared with the normal weight group， the overweight/obesity group had less yang deficiency constitution and blood stasis constitution， but more phlegm dampness constitution and damp heat constitution， and the differences were statistically significant （P<0.05）. In the male subgroup， there were significant differences in the prevalence of yang deficiency constitution， phlegm dampness constitution and special endowment constitution between the two groups （P<0.05）； In the female subgroup， there were significant differences in the prevalence of yang deficiency constitution and phlegm dampness constitution between the two groups （P<0.05）. ③Logistic regression analysis adjusted for the gender and 9 TCM constitutions that confounded each others showed that overweight/obesity was correlated with gender ［OR=3.39，95%CI（2.41～4.76），P<0.001］， yang deficiency constitution ［OR=0.46，95%CI（0.27～0.79），P=0.004］， phlegm dampness constitution inclination ［OR=2.37，95%CI（1.20～4.69），P=0.013］， and phlegm dampness constitution ［OR=3.35，95%CI（2.01～5.57），P<0.001］.ConclusionOverweight/obesity may be correlated with a variety of TCM constitution types， with the greatest correlation with phlegm-damp constitution and yang-deficiency constitution.关键词：overweight;obesity;TCM constitution;body mass index;phlegm-damp constitution;yang deficiency constitution;cross-sectional study
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发布时间：2022-10-26 - 摘要：Professor Zhang Yunling’s experience in treating generalized anxiety disorder （GAD） with six methods is introduced. According to Pro. Zhang， the pathogenic mechanisms of GAD include： Shaoyang stagnation and pivot malfunction； yin-yang imbalance and Jueyin wind-stirring； transformation failure due to spleen deficiency， retention and obstruction of damp turbidity； ying-wei dysregulation and exterior-interior complex； yin-blood deficiency and internal disturbance of deficiency heat； and pathogenic phlegm-heat disturbing internal organs. In the clinic， these pathogeneses are sometimes concurrent.Correspondingly， there are six treatment methods proposed which have achieved satisfactory results in the clinic， i.e.， pivoting qi movement of Shaoyang， calming wind-stirring of Jueyin， strengthening the spleen-earth of Taiyin， harmonizing the exterior-interior and regulating ying-wei， nourishing yin and blood to tranquilize the mind， and resolving phlegm and clearing heat. Two proven cases have presented for exemplification.关键词：generalized anxiety disorder;emotional disorder;mental disorder;traditional Chinese medicine therapy;famous doctor’s experience
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发布时间：2022-10-26 - 摘要：The clinical experience of Professor Yang Shuo’s “spirit-regulating acupuncture” for the treatment of emotional diseases is introduced. Professor Yang believes that the key to regulating the spirit lies in the qi and blood of the internal organs and in the abundance of yang qi. The main acupuncture points selected are five-zang back-shu points and five-zhi points together with Jiaji points.Given the difference between short-term onset and long-term onset of emotional diseases， shallow needling of back-shu points and five-zhi points or deep needling of Jiaji points should be emphasized accordingly during treatment.When applying shallow needling to the back-shu points and five-zhi points on the left side， the needle tip points to the head； when applying shallow needling to the back-shu points and five-zhi points on the right side， the needle tip points to the foot.Through the application of “spirit-regulating acupuncture”， the purpose of warming yang and nourishing spirit， coordinated regulation of spirit， five internal organs and qi movement can be realizen. One proven case is exemplified.关键词：emotional disease;insomnia;mental disorder;acupuncture;spirit-regulating;back-shu point;five-zhi point;Jiaji point
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发布时间：2022-10-26 - 摘要：Professor Chen Hongfeng’s clinical experience in treating postmenopausal and postoperative breast cancer patients is introduced in this article. It is considered that postmenopausal and postoperative breast cancer patients often have deficiency syndrome， with the lingering of pathogenic factors. In clinical practice， Professor Chen attaches importance to the syndrome differentiation of liver， spleen and kidney， and eliminates pathogenic factors through strengthening healthy qi， which is exemplified by 3 proven cases.关键词：breast cancer;postmenopause;perioperative period;famous doctor’s experience;traditional Chinese medicine therapy
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发布时间：2022-10-26 - 摘要：Based on the theory of turbid toxin， the pathogenesis and treatment of consumptive thirst（diabetes mellitus） were analyzed. Turbid toxin was not only a pathological product produced in the process of diabetes mellitus， but also a pathogenic factor participating in all stages of diabetes mellitus. From turbid gasification to poison was the basis for diabetes mellitus， and turbid toxins damaging the meridians was the core complications of diabetes mellitus. It was advocated using methods such as changing chaotic detoxification， clearing heat and detoxification， removing blood stasis detoxification， supporting energy and resolving toxin and other ways to guide turbidity ， then eliminating evil factors and supporting healthy energy.关键词：consumptive thirst;diabetes mellitus;turbid toxins;etiology and pathology;therapeutic principle and method;traditional Chinese medicine therapy
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发布时间：2022-10-26 - 摘要：ObjectiveTo study the intervention effect of Dahuang Mudan Decoction plus Sijunzi Decoction on pancreatic fibrosis induced by dibutyltin dichloride （DBTC） in rats.MethodsForty-eight male SD rats were randomly divided into a blank group， a DBTC model group， a Dahuang Mudan Decoction group， a Sijunzi Decoction group， a Dahuang Mudan Decoction plus Sijunzi Decoction group （referred to as “compound group”） and a coenzyme Q10 control group， with 8 rats in each group. The blank group was administered with caudal vein injection of normal saline （1 mL/kg）， other groups were injected with DBTC （8 mg/kg） intravenously in the tail vein. Starting from the second day of molding， the corresponding drugs administered by gavage at 1 mL per 100 g of rat body mass every day for 3 weeks. The rats in the Dahuang Mudan Decoction group were given Dahuang Mudan Decoction （raw drug content 0.63 g/mL）， those in the Sijunzi Decoction group were given Sijunzi Decoction （raw drug content 0.24 g/mL）， those in the compound group were given Dahuang Mudan Decoction plus Sijunzi Decoction （raw drug content 0.87 g/mL）， those in the control group were given Coenzyme Q10 （5 mg/kg）， and those in the blank and model groups were given equal volume of 0.9% NaCl solution by gavage. After the final administration， pancreatic tissues of rats were collected and serum was isolated. Serum alanine aminotransferase （ALT）， aspartate aminotransferase （AST）， albumin （ALB）， total bile acid （TBA）， creatinine （Cr）， urea （UREA） and interleukin-6 （IL-6） contents were detected. HE staining and Sirius scarlet staining were used to assess the pancreatic histological morphology， and immunohistochemistry were used to investigate the expression of type I collagen α1 （COLⅠA1） and α-smooth muscle actin （α-SMA）.ResultsCompared with the blank group， the activities of ALT and AST in the DBTC model group were significantly increased （P<0.05）. HE staining showed that the pancreatic glands in the DBTC model group were atrophic， with destruction of pancreatic ducts and acinar vesicles， fibrous tissue hyperplasia， a red-stained structureless area between some lobules， and a large number of inflammatory cells infiltrated in the pancreatic interstitium. Sirius scarlet staining showed that some of the destroyed and atrophied acinar structures in the pancreatic tissue of rats in the DBTC model group were replaced by collagen fibers， and the damaged acini， lobules and islets presented nodular fibrosis. The surface ratio （SR） of positive Sirius scarlet staining also increased significantly （P<0.05）. Compared with the model group， the ALT and AST activities of rats in each drug intervention group decreased to different degrees （P<0.05）； the SR of pancreatic tissue in each drug intervention group also decreased to different degrees （P<0.05）； and the differences in the expression of COLⅠA1 and α-SMA protein in pancreatic tissues of rats in each drug intervention group were statistically significant （P<0.05）. Compared with Dahuang Mudan Decoction group and Sijunzi Decoction group， the activities of ALT and AST in the compound group decreased （P<0.05）. Compared with the compound group，the differences in the expression of COLⅠA1 and α-SMA proteins in pancreatic tissues of rats in the Dahuang Mudan Decoction group， Sijunzi Decoction group and coenzyme Q10 group were statistically significant （P<0.05）.ConclusionDahuang Mudan Decoction plus Sijunzi Decoction can significantly improve DBTC induced pancreatic fibrosis in rats， and there is a certain degree of effect-enhancing mechanism through compatibility.关键词：chronic pancreatitis;pancreatic fibrosis;Dahuang Mudan Decoction;Sijunzi Decoction;compatibility of compound formula;rat model;traditional Chinese herbal medicine research
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发布时间：2022-10-26 - 摘要：ObjectiveTo observe the effect of acupoint catgut embedding on postoperative recurrence of polyps and metabolic level in patients with colorectal adenomatous polyps （CAP） complicated with metabolic syndrome （MS）.MethodsEighty patients with CAP （postoperative） complicated with MS were randomly divided into a treatment group （n=40） and a control group （n=40）. The control group was administered with symptomatic treatment and health management， while the treatment group was administered with acupoint catgut embedding besides the treatment measures of the control group. The treatment lasted 3 consecutive months. The recurrence of polyps in both groups was observed at 6 and 12 months after treatment，and the treatment safety was observed. The levels of serum total cholesterol （TC）， triacylglycerol （TG）， low-density lipoprotein cholesterol （LDL-C）， high-density lipoprotein cholesterol （HDL-C）， fasting plasma glucose （FPG）， and glycated hemoglobin （HbA1c）， as well as body mass， body mass index （BMI）， waist circumference （WC）， waist-to-hip ratio （WHR）， systolic blood pressure， and diastolic blood pressure were compared before and after treatment.Results①During the trial， there were 3 drop-out cases in the treatment group and 2 drop-out cases in the control group. Finally， 75 cases completed the trial， including 37 cases in the treatment group and 38 cases in the control group. ②At 6 months after treatment， there was no statistically significant difference in the postoperative recurrence rate of polyps between the two groups （P>0.05）. At 12 months after treatment， the postoperative recurrence rate of polyps was 13.5% in the treatment group and 34.2% in the control group， and the difference was statistically significant （P<0.05）. ③According to the intra-group comparison before and after treatment， the levels of serum LDL-C， HDL-C， FPG and HbA1c， as well as systolic blood pressure， body mass， BMI， WC and WHR decreased in the treatment group （P<0.05）； The levels of serum TC and LDL-C， as well as WC and WHR decreased in the control group （P<0.05）. After treatment， the levels of serum LDL-C， FPG and HbA1c， as well as systolic blood pressure， body mass， BMI， WC and WHR in the treatment group were lower than those in the control group （P<0.05）. ④No abnormal safety index was found in both groups.ConclusionsAcupoint catgut embedding has certain popularization value as it can prevent the postoperative recurrence of colorectal adenomatous polyps by regulating metabolic levels， and effectively prevent and treat common community diseases such as hypertension， diabetes and hyperlipidemia. The safety of this treatment is good.关键词：colorectal polyps;precancerous lesions;metabolic syndrome;acupoint catgut embedding;recurrence;colorectal cancer;clinical trial
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发布时间：2022-10-26 - 摘要：ObjectiveTo observe the inhibitory effect of Chaihu Shugan Powder on liver fibrosis in rats with chronic steatohepatitis， and to explore its effect on the regulation of Notch pathway in liver tissues.MethodsTotally 50 SD adult rats were fed with high-fat diet to establish chronic steatohepatitis in rats. The successful modelled rats were divided into model group， positive group， low， medium and high dose groups of Chaihu Shugan Powder by a random number table. Another 8 adult SD rats were recorded as normal group. The positive group was administrated with rosiglitazone 2 mg/kg， Chaihu Shugan Powder low， medium and high dose groups with 3.15， 6.30， 12.60 g/kg Chaihu Shugan Powder soup， and the model group and the normal group with 1 mL/ 100 g normal saline once a day for 10 weeks. Hematoxylin eosin （HE） staining was used to observe the modeling. The serum liver function was measured with ELISA before and after the intervention， and radioimmunoassay was used to detect the liver fibrosis indexes and contents of hydroxyproline （Hyp） after intervention. Hyp contents in liver tissues were detected by modified chloramine-T method. HE staining was used to observe the pathological changes of liver tissues. Hepatic fibrosis was observed by Sirius red staining. The mRNA and protein expressions of TGF-β1， Notch 1， Notch 3， hairy and enhancer of split 1 （Hes1）， α- smooth muscle actin （α-SMA） were detected by RT-qPCR and Western blot.ResultsThe levels of serum liver function and liver fibrosis indexes， Hyp content in liver tissues of the model group after intervention were higher than those of the normal group， while those in the positive group and Chaihu Shugan Powder low， medium and high dose groups were lower than those in the model group， and the levels in the low dose group of Chaihu Shugan Powder were higher than those in the positive group， while those in the high dose group of Chaihu Shugan Powder were lower than those in the positive group. And the above differences were statistically significant （P<0.05）. The pathological changes of liver fibrosis in the model group were significant， while those in the positive group and Chaihu Shugan Powder low， medium and high dose groups were alleviated， and the changes in the high dose group of Chaihu Shugan Powder were most similar to those of the normal group. After intervention， the relative expressions of TGF-β1， Notch3， Hes1， α-SMA mRNAs and proteins in liver tissue in the model group were higher than those in the normal group， while those in the positive group and Chaihu Shugan Powder low， medium and high dose groups were lower than those in the model group， and those in the low dose group of Chaihu Shugan Powder were higher than those in the positive group， while the expressions in the high dose group of Chaihu Shugan Powder were lower than those in the positive group. And the changes of Notch 1 mRNA and protein were on the contrary. The above differences had highly statistically significant （P<0.05）.ConclusionIntragastric administration of Chaihu Shugan Powder to rats with chronic steatohepatitis can alleviate liver damage and inhibit hepatic fibrosis， which may be related to the regulation of Notch pathway， inhibition of TGF-β1， Notch3， Hes1， α-SMA expression and up-regulation of Notch1 expression.关键词：chronic hepatitis;fatty liver;liver fibrosis;Chaihu Shugan Powder;Notch pathway;rat model;traditional Chinese herbal medicine research
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发布时间：2022-10-26 - 摘要：ObjectiveTo study the effects of Wenhua Juanbi Recipe （WJR） on inflammation and m6A methylation in rats with collagen-induced arthritis （CIA）.MethodsFifty female Wistar rats were randomly divided into two groups： a normal group （n=10） and a model group （n=40）. CIA model was established by intracaudal multi-point injection of emulsifier. The model was evaluated by volumetric method （drainage volume） and arthritis index （AI） scoring method to assess the degree of toe swelling and joint inflammation， and the model with toe swelling> 1.6 mL and AI score≥4 was considered as successful. Thirty successfully modeled CIA rats were randomly divided into a model group （n=10）， a WJR group （TCM group， n=10） and a MTX group （n=10）. The TCM group was administered with WJR at a dose of 22.9 g·kg-1·d-1； and the MTX group was given methotrexate suspension （MTX） at a dose of 0.78 mg·kg-1 once a week. The normal group and the CIA group were given equal amounts of 0.9% NaCl solution once a day. The synovial membrane of the knee of rats was taken from each group after 30 days of treatment. The synovial pathology was examined by HE staining. The gene expressions of Mettl3， Mettl14， Fto， Alkbh5， Ythdf1 and Ythdf2 and the expression levels of joint inflammatory factors TNF-α， IL-1β and IL-6 genes in synovial membrane were detected by real-time PCR. Western blot was used to detect the expressions of Mettl3 and Fto proteins.ResultsCompared with the condition in the normal group， the toe swelling degree and AI score of rats in the model group increased significantly， and in the model group the expressions of Mettl3， Mettl14， Fto， Alkbh5 and Ythdf1 genes increased， the expression of Ythdf2 gene decreased， the expressions of TNF-α， IL-1β and IL-6 genes increased， and the expressions of Mettl3 and Fto proteins increased （P<0.05）. Compared with the condition in the model group， the toe swelling degree and AI score of rats in the WJR group and the MTX group decreased gradually with time， and in the WJR group and the MTX group the expressions of Mettl3， Mettl14， Fto， Alkbh5 and Ythdf1 decreased， the expression of Ythdf2 increased， the expressions of TNF-α， IL-1β and IL-6 genes decreased， and the expressions of Mettl3 and Fto proteins decreased （P<0.05）. Compared with the condition in the MTX group， the expressions of Mettl3， Mettl14， Fto， Alkbh5 and Ythdf1 decreased， the expression of Ythdf2 gene increased， the expressions of TNF-α， IL-1β and IL-6 genes decreased， and the expressions of Mettl3 and Fto proteins decreased in the WJR group （P<0.05）.ConclusionWenhua Juanbi Recipe may have a therapeutic effect on CIA by affecting the m6A methylation to exert anti-inflammatory effects.关键词：rheumatoid arthritis;collagen induced arthritis;Wenhua Juanbi Recipe;m6A;rat model;traditional Chinese herbal medicine research
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发布时间：2022-10-26 - 摘要：ObjectiveTo observe the effect of Modified Taohong Siwu Decoction （MTHSWD） on JAK2-STAT3 pathways in rat model of retinal vein occlusion （RVO）.MethodsNinety SD rats aged 8 weeks were randomly divided into blank control group （group A）， model control group （group B）， MTHSWD high concentration group （group C）， MTHSWD medium concentration group （group D）， MTHSWD low concentration group （Group E） with 18 rats in each group. Group A was left untreated， and the right eyes of rats in other groups were treated with laser combined with Rose Bengal to establish the RVO model in SD rats. After successful modeling， groups A and B were given 2 mL of sterile injection by gavage， and groups C， D and E were given low （10 mg/kg）， medium （20 mg/kg） and high （40 mg/kg） concentrations of MTHSWD by gavage. The retinal structure， thickness and edema of ganglion cell layer （GCL） were observed by HE staining at 7， 14 and 21 days after administration. The protein expressions of Janus kinase protein 2 （JAK2）， Janus kinase protein 2 phosphorylation （P-JAK2）， signal transduction and transcription factor 3 （STAT3）， signal transduction and transcription factor 3 phosphorylation （P-STAT3） in retina were detected by Western blot method.Results①Retinal structure： The structure of three layers of retina in each group was intact on the 7th day， and partial recanalization of thrombus in the retinal vein was found in each group on the 14th day， and there was no significant change in the number of ganglion cells in each group on the 21st day. Retinal thickness： The gap of retinal nerve fiber layer was broadened in group B， C and E on the 7th day， and the retinal thickness became thinner in group C， D and E on the 14th and 21st day. GCL edema： GCL edema in each group on the 7th day and the edema in C， D and E groups on the 14th day alleviated， and GCL had no edema on the 21st day. ②Expression of JAK2 protein： Compared with group A， the expression of JAK2 protein in group B was lower （P<0.05）， the expression of JAK2 protein in group C and D was higher than that in group B （P<0.05）， and the expression of JAK2 protein in group C and D was higher than that in group E （P<0.05）. The expression of P-JAK2 protein： Compared with group A， the expression of P-JAK2 in group B and E was higher （P<0.05）， the expression of P-JAK2 in group C， D and E was lower than that in group B （P<0.05）， and the expression of P-JAK2 in group C and D was lower than that in group E （P<0.05）. The expression of STAT3 protein： Compared with group A， the expression of STAT3 protein in group D decreased， and the expression of STAT3 protein increased in group E （P<0.05）. Compared with group B， the expression of STAT3 protein decreased in group C and increased in group E （P<0.05）. Compared with group E， the expression of STAT3 protein in group C and D was lower （P<0.05）. The expression of P-STAT3 protein： Compared with group A， the expression of P-STAT3 protein in group B and C was higher （P<0.05）， and the expression of P-STAT3 protein in group C was lower than that in group B （P<0.05）.ConclusionModified Taohong Siwu Decoction can improve fundus ischemia and hypoxia and block the development of RVO， and its mechanism may be related to the inhibition of JAK2-STAT3 pathway activation.关键词：retinal vein occlusion;Modified Taohong Siwu Decoction;JAK2;STAT3;rat model;traditional Chinese herbal medicine research
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发布时间：2022-10-26 - 摘要：ObjectiveTo investigate the effect and possible mechanism of Qingfei Tongluo Formula on oxidative stress injury after Mycoplasma pneumoniae （MP） infection of human alveolar basal epithelial cells （A549 cells）.MethodsA549 cells were divided into a blank group， an MP infected group （MP concentration was 1×107 CFU·mL-1， and Qingfei Tongluo Formula groups at different concentrations （the concentrations of Qingfei Tongluo Formula were 0.5 mg·L-1， 1.0 mg·L-1， 2.0 mg·L-1， respectively）. After the treatment of Qingfei Tongluo Formula for 3 days， the morphological changes of cells in each group were observed by inverted microscope， the contents of intracellular reactive oxygen species （ROS） were detected by 2'，7'-dichlorofluorescein acetate （DCFH-DA） fluorescence staining， and the expression levels of nuclear transcription factor E2-related factor 2 （Nrf2）， heme oxygenase-1 （HO-1）， cytoplasmic chaperone molecule （Keap1） and quinone oxidoreductase （NQO1） were detected by Western blot. A549 cells were divided into a blank group， an MP infection group， an MP + siNC group， an MP + Qingfei Tongluo Formula group， an MP + siNrf2 group， and an MP + siNrf2 + Qingfei Tongluo Formula group. The cells were transfected after being treated with Qingfei Tongluo Formula for 3 days， and the expression levels of Nrf2， Keap1， HO-1 and NOQ1 were detected by Western blot.ResultsA suitable concentration of Qingfei Tongluo Formula up-regulated the protein expression of Nrf2， Keap1， HO-1 and NOQ1 in A549 cells after MP infection and decreased the intracellular ROS level， which in turn inhibited cell apoptosis. After inhibiting the expression of Nrf2， the antioxidant effect of Qingfei Tongluo formula decreased.ConclusionQingfei Tongluo Formula can attenuate oxidative injury in A549 cells after MP infection， and the mechanism may be related to the regulation of Nrf2/ARE signaling pathway.关键词：mycoplasmal pneumoniae pneumonia;Qingfei Tongluo Formula;oxidative stress;A549 cells;Nrf2/ARE signaling pathway;mechanism of function;traditional Chinese herbal medicine research
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发布时间：2022-10-26 - 摘要：ObjectiveTo investigate the effects of Tuina combined with spinal cord electrical stimulation （SCS） on the regulation of apoptosis and the expression of B-cell lymphoma-2 （Bcl-2） and Bcl-2-associated X-protein （Bax） in anterior horn motor neurons of the spinal cord in rats with sciatic nerve injury （SNI）.MethodsTwenty-four clean SD rats were randomly divided into a sham operation group （n=8）， a model group （n=8） and a treatment group（n=8）. SNI model was established in 16 rats except those in the sham operation group， and interventions began 7 d after the model was established. Behavioral changes of rats and morphological changes of nerve fibers were observed at 21 d after modeling， and the neuronal cell morphology and expression of Bcl-2 and Bax proteins in the anterior horn of the spinal cord of L4~L6 segments were observed by Nissl staining and immunohistochemistry. The number of apoptotic neurons was detected by TUNEL assay.ResultsCompared with rats in the sham operation group， the modeled rats showed swelling and deformation of nerve fibers and functional impairment. Compared with rats in the model group， rats in the treatment group showed significantly increased sciatic function index （SFI） at 21 d after modeling （P<0.05）. Compared with rats in the sham operation group， modeled rats showed neuron apoptosis in anterior horn of the spinal cord； The number of apoptotic neurons in anterior horn of the spinal cord in treatment group was significantly lower than that in the model group （P<0.05）. The Bcl-2 protein expression was significantly higher （P<0.05） and Bax protein expression was significantly lower （P<0.05） in anterior horn neurons of the spinal cord in the treatment group than those in the model group.ConclusionTuina combined with SCS can promote the recovery of nerve function in SNI rats， protect injured spinal cord neurons， and may be involved in the anti-apoptosis process of neurons after sciatic nerve injury through up-regulating Bcl-2 protein expression and down-regulating Bax protein expression.关键词：peripheral nerve injury;rehabilitation;Tuina;spinal cord electrical stimulation;sciatic nerve;nerve regeneration;cell apoptosis;rat model
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发布时间：2022-10-26 - 摘要：Reviewed the domestic and foreign research in the past 40 years， it was found that sublingual collaterals diagnosis was widely used in a variety of clinical diseases such as digestive system， cardiovascular and cerebrovascular system， endocrine system， tumor， respiratory system， gynecology and so on. Sublingual collaterals diagnosis had practical value in diagnosis and prognosis of different diseases.关键词：tongue diagnosis;collaterals;diagnostics of traditional Chinese medicine;clinical application;research progress
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发布时间：2022-10-26 - 摘要：This paper summarized the application of surface electromyography in acupuncture acupoint selection， acupoint specificity， acupoint deqi， acupuncture manipulation research， and acupuncture efficacy evaluation. Surface electromyography technology could dynamically and quantitatively reflect neuromuscular electromyography signals in real time， and could objectively and accurately evaluate the efficacy of acupuncture from the characteristic parameters of surface electromyography signals. Therefore， this technology was widely used in diagnosis and efficacy evaluation of neuromuscular diseases such as muscle fatigue， neuromuscular dysfunction after stroke， dysphagia， etc.关键词：surface electromyography;acupuncture;acupoint specificity;neuromuscular disease;stroke;therapeutic evaluation;research progress
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发布时间：2022-10-26 - 摘要：Vascular cognitive impairment （VCI） is a clinical syndrome of clinical stroke or subclinical vascular brain injury involving impairment of at least one cognitive domain as a result of cerebrovascular lesions and their risk factors. Microglia are the main regulators and releasers of neuroinflammation in the brain， which have the dual functions of neurocytoprotection and neurotoxicity. Recent studies have found that they can be used as an important marker to evaluate the condition of VCI and predict the prognosis. The intervention of microglia through traditional Chinese herbal medicines （extracts， preparations， and compound prescriptions） to treat vascular cognitive impairment has received considerable attention in recent years and has made some research progress， but further in-depth studies are still needed.关键词：cognitive impairment;dementia;stroke;microglia;inflammation;traditional Chinese herbal medicine;research progress
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发布时间：2022-10-26 - 摘要：This paper reviews the research progress of Chaihu plus Longgu Muli Decoction against depression. Chaihu plus Longgu Muli Decoction can reconcile Shaoyang and calm the nerves. It has been applied in various depressive disease including depression， tumor-related depression， cardiovascular disease with depression， neurological disease with depression， withdrawal depression， obstetrics and gynecology with depression， digestive system disease with depression. Its therapeutic mechanism may be related to regulating the expression of neurotransmitters and their receptors， adjusting the hypothalamic-pituitary-adrenal axis， protecting neuronal cells， improving the immune status of the body， and reducing the secretion of inflammatory factors.关键词：depression;Chaihu plus Longgu Muli Decoction;Chinese classic formulas;clinical application;mechanism research;research progress
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发布时间：2022-10-26
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