最新刊期
期 5 ， 2021
- 摘要：ObjectiveTo compare the differences between the International Classification of Traditional Medicine (ICTM) in the Eleventh Revision of International Classification of Diseases (ICD-11) and the new editions of Traditional Chinese Medicine (TCM) National Standards, and promote the application of ICTM and the new editions of TCM National Standards.MethodsRelational mappings were created for identifying differences between ICTM and the new editions of TCM National Standards from the aspects of inclusion scope, coding rules, and classification system through mathematical statistics, text comparison, and literature analysis.ResultsIn respect of inclusion scope: ICTM and the new editions of TCM National standards have 169 common terms of diseases and 196 common terms of syndromes, but they actually share the remaining 141 terms of diseases and 175 terms of syndromes in common after excluding invalid codes. In respect of coding rules: In ICTM, Y and Z are used separately to mark "other specific" terms and "unspecified" terms, and a single column is used to mark valid codes, invalid codes and other rules; The new editions of TCM National Standards use a dot "." to indicate the classification hierarchy of terms, and the category attribute of each term is identified by the dot "." at the end of the code. In respect of classification system: Compared with ICTM, the new editions of TCM National Standards are more comprehensive in disease and syndrome classification system, which basically cover the syndrome classification system of ICTM.ConclusionsThere is significant difference in the number of terms included in ICTM and the new editions of TCM National Standards, and ICTM does not include terms of treatment. In respect of term compilation, classification and definition, the new editions of TCM National Standards either basically cover ICTM terms, or create relational mappings with ICTM terms.关键词：ICD-11;ICTM;Classification and Codes of Diseases and Patterns of Traditional Chinese Medicine;Clinic Terminology of Traditional Chinese Medical Diagnosis and Treatment;TCM standardization;TCM internationalization;TCM syndrome term;TCM disease term
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发布时间：2022-08-03 - 摘要：This paper introduced Professor Jiang Jian’s academic experience in diagnosing and treating aversion to cold due to depression syndrome mainly by releasing depression. It is considered that aversion to cold due to depression syndrome is a kind of masked depression syndrome under the cloak of aversion to cold, which has the characteristics of emotional disease, and the symptom of aversion to cold is weird and eccentric, also accompanied by the symptoms of autonomic nerve dysfunction. Furthermore, treating the disease from depression aspect is worked. Fully understanding and mastering the morphology of depression syndrome and abandoning the narrow fixed thought on the etiology, pathogenesis and therapeutic prescriptions of cold syndrome are the two magic weapons to improve the level of diagnosis and treatment of aversion to cold due to depression.关键词：cold syndrome;depression syndrome;aversion to cold due to depression syndrome;masked depression syndrome;soothing the liver and regulating qi;nourishing the heart to tranquilize spirit
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发布时间：2022-08-03 - 摘要：The empirical facts in traditional Chinese medicine (TCM) can usually be recognized and utilized by western medicine, but the theory of TCM is difficult to be assimilated or shaped, which has become the core symbol of the existence and uniqueness of TCM. In theory, TCM and western medicine present two different worldviews, and the essence of theoretical differences lies in the differences in concepts and conceptual networks. The transformation of scientific worldview will not occur just because of an outstanding research discovery. In order to achieve self-realization of TCM worldview, necessary conditions need to be provided, such as discovering and revealing abnormal events in modern medical research, preparing alternative theories, and predicting accurately, etc. Only when we realize that the core goal of our research is to embody the worldview of TCM can we truly embark on the road of TCM research from proof research to original research.关键词：traditional Chinese medicine;research paradigm;worldview
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发布时间：2022-08-03 - 摘要：ObjectiveBased on modern medical cases, this paper explored the rules of traditional Chinese medicine for nephrotic syndrome in children and explored the idea of applying high-frequency drugs in combination.MethodsThe literature about Chinese medicine treatment of nephrotic syndrome in children from the database of CNKI, CBM, VIP, and Wan Fang data built to April 2020 was used as the data source, and factor analysis, cluster analysis and association analysis of medicines were applied to explore the idea of application of high frequency drugs.ResultsAmong the 116 prescriptions and 212 Chinese medicines that met the inclusion criteria, most of them were tonifying deficiency medicine. Among them, Poria, Astragali Radix, Alismatis Rhizoma and Atractylodis Macrocephalae Rhizoma etc. were used more frequently.Most of the channel tropism were spleen, lung and kidney. Using both cold and temperature medicine, the dosage was careful.ConclusionThe treatment of nephrotic syndrome in children should pay attention to the physiological characteristics of children, and the medication should be mainly used to tonify deficiency, often combined with the treatment methods of diuresis, dispelling wind and relieving symptoms, clearing heat and invigorating blood, and astringency and consolidation.关键词：nephrotic syndrome in children;medical cases;medication rules;traditional Chinese medicine;data mining
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发布时间：2022-08-03 - 摘要：This paper introduces Professor Huang Shilin’s clinical experience in treating primary myelofibrosis (PMF) based on the pathological feature of "bone depletion and marrow deficiency" .Professor Huang believes that PMF belongs to the category of "Gu Wei" (atrophic debility of bones)in traditional Chinese medicine (TCM), and its pathological feature is "bone depletion and marrow deficiency" . The basic pathogenesis is intermingled toxin and blood stasis, kidney asthenia and essence deficiency. Professor Huang has developed a distinctive treatment plan based on syndrome differentiation and staging treatment. He divides PMF into intermingled toxin and blood stasis syndrome and kidney asthenia and essence deficiency syndrome. The former is treated with the method of removing toxin and clearing heat, removing blood stasis and resolving stagnation; while the latter is treated with the method of strengthening body resistance and eliminating pathogenic factors, consolidating kidney and supplementing essence. Professor Huang also differentiates PMF into progressive stage and stable stage, and adopts integrated traditional Chinese and western medicine for treatment. To sum up, the therapeutic principles of removing toxin and resolving stasis, strengthening body resistance and eliminating pathogenic factors should be followed throughout the whole treatment process.关键词：primary myelofibrosis;malignant tumor;blood diseases;atrophic debility of bones;removing toxin and resolving stasis;strengthening body resistance and eliminating pathogenic factors;famous doctor’s experience;Huang Shilin
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发布时间：2022-08-03 - 摘要：Professor Su Xiao’s experience in treating Sjogren’s syndrome from Sanjiao was summarized. Professor Su believed that the main pathogenesis of Sjogren’s syndrome was the external or internal injury, which led to yin deficiency in the viscera, injury fluid by heat, qi depression in Sanjiao and blood stasis. All those pathogenic factors resulted in the loss of viscera in nourishing. The treatment was based on self-made Yangyin Huoxue Shengjin Prescription, which was modified by syndromes combined with the physiological characteristics of Sanjiao and different disease positions.关键词：Sjogren’s syndrome;Sanjiao;expert experience;Su Xiao
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发布时间：2022-08-03 - 摘要：This paper introduces the clinical experience of electrocautery needling (a therapy improved from traditional Chinese medicine fire needling) in treating vitiligo in stable stage. We believe that compared with traditional fire needling, electrocautery needling has the following advantages: lower risk of scald and infection, higher treatment efficiency and less pain feelings. Meanwhile, based on a preliminary clinical observation on the condition of a small sample of patients before and after treatment, it was found that electrocautery needling and excimer laser phototherapy had an equivalent effect on inducing the repigmentation of white depigmented macules in stable stage. The rate of repigmentation of vitiligo white macules mainly depends on the location. Those presenting on the skin of the head and face have better curative effect as they are more sensitive, while those at hands and feet have relatively worse curative effect as they are less sensitive. One proven case is provided.关键词：electrocautery needling;fire needling therapy;excimer laser phototherapy;vitiligo;disfiguring dermatosis;external treatment of traditional Chinese medicine
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发布时间：2022-08-03 - 摘要：Through textual research on Scutellariae Radix in past dynasties, and analyzing its ancient and modern medication situation, origins and producing areas, it is inferred that Scutellariae Radix used in Treatise on febrile Diseases is Scutellaria baicalensis Georgi with longer growth time or Scutellaria baicalensis Georgi with shorter growth time. Combined with modern pharmacology, ultra-high liquid chromatography fingerprint and pattern recognition analysis, it is found that Scutellariae Radix used in Treatise on febrile Diseases is Scutellaria baicalensis Georgi with longer growth time.关键词：Scutellariae Radix;Treatise on Febrile Diseases;Scutellaria baicalensis Georgi with longer growth time
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发布时间：2022-08-03 - 摘要：This paper introduced Professor Huang Huang’s clinical experience in the application of Fangfeng Tongsheng Powder through three medical cases. It is considered that Fangfeng Tongsheng Powder is suitable for the syndrome of external exuberance of pathogenic wind with internal pathogenic heat, characterized by simultaneous excess in the exterior and interior, and clinically Professor Huang Huang emphasizes the combination of constitution identification, syndrome differentiation and disease differentiation, and pays attention to the important clinical value of correspondence between prescription and syndrome and selection of prescription based on patient’s constitution.关键词：Fangfeng Tongsheng Powder;constitution identification;classical prescriptions;experience of famous doctors;Huang Huang
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发布时间：2022-08-03 - 摘要：ObjectiveTo observe the efficacy of Shuanglong Antifiber Capsule (SAC) in patients with connective tissue disease associated with pulmonary fibrosis and its effect on serum KL-6 and T lymphocyte subsets in peripheral blood.MethodsA total of 80 patients with pulmonary fibrosis in connective tissue disease were randomly divided into treatment group and control group, 40 cases in each group. On the basic treatment, the treatment group was treated with SAC, and the control group was treated with placebo. The treatment course was 12 weeks. The TCM syndrome score, high resolution CT(HRCT) score, peripheral blood T lymphocyte subsets (CD4+, CD8+) levels and serum levels of KL-6 were compared between the two groups.Results①During the treatment, 2 cases were lost and 38 cases were completed the experiment in each group.②After treatment, the TCM syndrome scores and serum KL-6 level of both groups were decreased (P<0.05), but the HRCT scores of both groups were no significant changes (P>0.05). The TCM syndrome scores and serum KL-6 level in the treatment group were lower than those in the control group (P<0.05).But the HRCT scores were no statistical difference between the two groups (P>0.05). ③After treatment, CD4+T lymphocytes and CD4+/CD8+ in the treatment group were increased (P<0.05), and CD8+ T lymphocytes were decreased (P<0.05), while there were no significant changes in CD4+T lymphocytes, CD8+ T lymphocytes and CD4+/CD8+ in the control group (P>0.05).After treatment, CD4+T lymphocytes, CD8+T lymphocytes and CD4+/CD8+ in the treatment group were better than those in the control group (P<0.05).ConclusionsSAC had good efficacy in the treatment of connective tissue disease associated with pulmonary fibrosis. The mechanism might be associated with the decreased expression of KL-6 which secreted by alveolar epithelial cells Ⅱ. In addition, SAC could improve CD4+T lymphocytes and decrease CD8+T lymphocytes of the peripheral blood to adjust cellular immunity.关键词：Shuanglong Antifibrin Capsule;connective tissue disease;pulmonary fibrosis;KL-6;T lymphocyte subsets;clinical observation
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发布时间：2022-08-03 - 摘要：ObjectiveTo observe the clinical effect of modified Shaoyao Gancao Decoction combined with pregabalin on patients with fibromyalgia syndrome (FMS) of liver depression and spleen deficiency syndrome.MethodsSixty-four patients with FMS of liver depression and spleen deficiency syndrome were randomly divided into treatment group (n=32) and control group (n=32). The control group was administered with pregabalin, while the treatment group was administered with Shaoyao Gancao Decoction besides pregabalin. The course of treatment in both groups was 1 month. The clinical effect and incidence of adverse reactions were observed, and the changes of scores of visual analogue scale (VAS), TCM syndrome, Fibromyalgia Impact Questionnaire (FIQ), Pittsburgh Sleep Quality Index (PSQI) and the 36-item Short Form (SF-36) Health Survey were compared.Results①The total effective rates of the treatment group and the control group were 96.88% and 75.00%, respectively, and the clinical effect of the treatment group was better than that of the control group (P<0.05). ②The VAS scores, TCM syndrome scores, FIQ scores and PSQI total scores of both groups decreased after treatment (P<0.05) and the VAS scores, TCM syndrome scores, FIQ scores and PSQI total scores in the treatment group were lower than those in the control group (P<0.05). ③The scores of each item in SF-36 Health Survey in the treatment group increased after treatment (P<0.05), while in the control group only the scores of physiological function, somatic pain and psychological health items increased (P<0.05); There were significant differences in the scores of each item in SF-36 Health Survey between the groups after treatment (P<0.05). ④The incidence of adverse reactions in the treatment group and the control group were 15.63% and 12.50% respectively, with no significant difference between the groups (P>0.05).ConclusionModified Shaoyao Gancao Decoction and pregabalin have synergistic effects, Thus the combined use has satisfactory therapeutic effect on patients with FMS of liver depression and spleen deficiency syndrome by better relieving clinical symptoms such as pain, and improving sleep and quality of life of patients with good safety.关键词：fibromyalgia syndrome;Shaoyao Gancao Decoction;prebahrain;somatic symptom;psychological symptom
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发布时间：2022-08-03 - 摘要：ObjectiveTo observe the clinical effect of modified Wuhu Zhuifeng Powder on non-motor symptoms of Parkinson’s disease (PD) patients with kidney deficiency and blood stasis syndrome.MethodsEighty-four PD cases of kidney deficiency and blood stasis syndrome were randomly divided into control group (n=42) and treatment group (n=42). The control group was administered with levodopa and benserazide hydrochloride tablets, while the treatment group was additionally administered with modified Wuhu Zhuifeng Powder besides the basic treatment of the control group. The course of treatment in both groups was 3 months. The clinical effect and medication safety were observed, and the changes of scores of Non-motor Symptom Quest (NMS-Quest), Hamilton Depression Scale (HAMD), Parkinson’s Disease Sleep Scale (PDSS), and Mini-Mental State Examination (MMSE), as well as the changes of serum adiponectin (APN) and uric acid (UA) levels were compared.Results①During the trial, there was no drop-out case in both groups, and 84 cases finally completed the trial. ②The total effective rates of the treatment group and the control group were 83.33% and 26.19% respectively; The difference in the clinical effect between two groups was statistically significant (P<0.05). ③The scores of NMS-Quest and HAMD in the two groups were significantly different before and after treatment (P<0.05), and the scores in the treatment group decreased significantly and those in the control group increased significantly. After treatment, the scores of NMS-Quest and HAMD in the treatment group were significantly lower than those in the control group (P<0.05). ④There were significant differences in PDSS and MMSE scores in the treatment group before and after treatment (P<0.05), while there was no significant difference in the control group before and after treatment (P>0.05). After treatment, the scores of PDSS and MMSE in the treatment group were significantly higher than those in the control group (P<0.05). ⑤There were significant differences in APN and UA levels in the treatment group before and after treatment (P<0.05), while there was no significant difference in the control group before and after treatment (P>0.05). After treatment, the levels of APN and UA in the treatment group were significantly higher than those in the control group (P<0.05). ⑥There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05).ConclusionCombined use of modified Wuhu Zhuifeng Powder can significantly relieve non-motor symptoms such as depression, insomnia, cognitive dysfunction in patients with Parkinson’s disease of kidney deficiency and blood stasis syndrome, and up-regulate the serum levels of APN and UA with satisfactory medication safety.关键词：Parkinson’s disease;kidney deficiency and blood stasis syndrome;traditional Chinese medicine therapy;modified Wuhu Zhuifeng Powder;non-motor symptom;depression
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发布时间：2022-08-03 - 摘要：ObjectiveTo explore the clinical efficacy of acupuncture and medicine combined with enhanced recovery after surgery (ERAS) on promoting the rehabilitation of patients undergoing laparoscopic colorectal cancer surgery.MethodsOne hundred and twenty patients underwent laparoscopic colorectal radical resection of colorectal cancer between January 2019 and August 2020 were randomly divided into the acupuncture and medicine (AM) group (62 cases) and the traditional treatment (TT) group (58 cases). The patients in the TT group were treated only with ERAS, while the patients in the AM group were treated additionally with electroacupuncture at Zusanli point after 6 hours of operation, and Rensheng Yiyi Fuzi Baijiang Powder after 12 hours of operation, with a course of 3 days. Compare the first aerofluxus time, first defecation time, oral feeding time, score of Chinese medical syndrome, level of prealbumin and albumin, postoperative complications, hospital stay and hospitalization expenses between two groups.ResultsThe first aerofluxus time, first defecation time and oral feeding time of the AM group were significantly shorter than those of the TT group (P<0.05). The score of Chinese medical syndrome of the AM group was lower than that of the TT group (P<0.01). The level of prealbumin and albumin was the same between the two groups before operation and on the first postoperative day(P>0.05), however, they were higher in the AM group on the fifth postoperative day(P<0.05). The total postoperative complications, hospital stay and hospitalization expenses of the AM group were less than those of the TT group (P<0.05).ConclusionAcupuncture and medicine combined with ERAS can accelerate the recovery of gastrointestinal function, improve nutritional status, decrease postoperative complications, shorten hospital stay and save hospitalization expenses for patients who undergo laparoscopic surgery for colorectal cancer.关键词：ERAS;Rensheng Yiyi Fuzi Baijiang Powder;electroacupuncture;Zusanli point;clinical research
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发布时间：2022-08-03 - 摘要：ObjectiveTo observe the effect of clearing heat and removing blood stasis traditional Chinese medicine on the inflammation of patients with adenomyosis after operation of high intensity focused ultrasound (HIFU).MethodsSixty patients with adenomyosis treated by HIFU were included and randomly divided into the control group and the treatment group, 30 cases in each group. The patients in the control group were treated with subcutaneous injection of gonadotropin releasing hormone analogue (GnRH-a) on the first day after operation; the patients in the treatment group were treated with clearing heat and removing blood stasis traditional Chinese medicine based on the treatment of the control group from the first day after operation with a course of 7 days. On the first day after operation (before treatment) and the tenth day after operation (after treatment), the scores of Chinese medical syndrome of the two groups were compared, and the effect of Chinese medical syndrome in both groups were evaluated; the levels of cancer antigen125 (CA125), vascular endothelial growth factor (VEGF), soluble vascular cell adhesion molecule-1 (sVCAM-1) and the inflammatory factors including interleukin (IL)-1, IL-6 and prostaglandin E2 (PGE2) in both groups were tested and compared.Results①After treatment, the total effective rates of the treatment group and the control group were 96.7% and 83.3% respectively, and the effect of the treatment group was superior to the control group (P<0.05). ②After treatment, the total scores of Chinese medical syndrome in both groups were significantly decreased (P<0.05), and the score of the treatment group was lower than that of the control group (P<0.05); the scores of all the symptoms in both groups were significantly decreased (P<0.05), and the scores of abdominal pain and sacrum discomfort symptoms in the treatment group were lower than those in the control group (P<0.05). ③After treatment, the levels of VEGF and sVCAM-1 in both groups were significantly decreased (P<0.05), the level of CA125 in the control group was increased (P<0.05), and the levels of VEGF and sVCAM-1 in the treatment group were lower than those in the control group (P<0.05). ④After treatment, the levels of IL-6 and PGE2 in the treatment group were decreased (P<0.05), the level of IL-1 in the control group was increased (P<0.05), and the level of IL-1 in the treatment group was lower than that in the control group (P<0.05).ConclusionClearing heat and removing blood stasis traditional Chinese medicine can improve the Chinese medical syndrome, reduce the levels of inflammatory factors and control the abnormal proliferation of blood vessels in patients with adenomyosis after operation of HIFU, thus improve the inflammation after operation of HIFU.关键词：adenomyosis;inflammatory factor;high intensity focused ultrasound;clearing heat and removing blood stasis
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发布时间：2022-08-03 - 摘要：ObjectiveTo explore the effect of traditional Chinese medicine (TCM) Sanyin Decoction on postoperative disease-free survival (DFS) of patients with triple-negative breast cancer (TNBC).MethodsA retrospective cohort study was conducted by enrolling 156 TNBC patients in the TCM-exposed group (treatment with Sanyin Decoction involved) and 192 TNBC patients in the non-TCM-exposed group (treatment without Sanyin Decoction involved). The DFS rates at 6 months, 12 months, 18 months and 24 months after operation were compared between the two groups, the survival curve was drawn with the Kaplan-Meier Plotter, and a Cox regression model was established to analyze the factors affecting the recurrence and metastasis of TNBC after operation.Results①There was no significant difference in the DFS rate between the two groups at 6 months and 12 months after operation (P>0.05). The DFS rates of the TCM-exposed group and the non-TCM-exposed group were 98.08% and 92.19% respectively at 18 months after operation, and the difference between the groups was statistically significant (P<0.05). The DFS rates of the TCM-exposed group and the non-TCM-exposed group were 98.08% and 89.06% respectively at 24 months after operation, and the difference between the two groups was statistically significant (P<0.05). ②The results of Cox regression analysis showed that the staging of metastatic lymph nodes (P<0.001) and TCM intervention (P<0.001) were factors affecting the recurrence and metastasis of TNBC patients after operation, among which TCM intervention was a protective factor [HR=0.191 (95% CI: 0.083~0.443) ], the staging of metastatic lymph nodes was a risk factor, and the higher the grade is, the higher the risk of recurrence and metastasis will be. Age of onset, pathological grade, tumor size, percentage of Ki-67-positive cells, pathological type and with/without neoadjuvant chemotherapy were not related to prognosis (P>0.05).ConclusionSanyin Decoction has an adjuvant therapeutic effect on TNBC, which is beneficial to the improvement of DFS. The staging of metastatic lymph nodes is a risk factor for postoperative recurrence and metastasis of TNBC, while the intake of Sanyin Decoction is a protective factor.关键词：triple-negative breast cancer;Sanyin Decoction;survival analysis;cohort study
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发布时间：2022-08-03 - 摘要：ObjectiveTo observe the clinical effect of modified Tuduzi Buqi Decoction combined with western medicine conventional therapy on the treatment of refractory ascites in liver cirrhosis.MethodsFifty-seven patients with refractory ascites in liver cirrhosis were divided into treatment group (n=32) and control group (n=25). The control group was administered with western medicine conventional therapy, while the treatment group was additionally administered with Tuduzi Buqi Decoction besides the western medicine conventional therapy. The course of treatment in both groups lasted 14 days. We compared the changes of ascites related indicators (24-hour urine volume, body mass, abdominal circumference, and ascites depth in supine position) and laboratory related indicators (liver function, renal function, electrolyte, and coagulation function).Results①According to the intra-group comparison before and after 7 days of treatment, in the treatment group the 24-hour urine volume increased (P<0.05), and the body mass, abdominal circumference, and depth of ascites in supine position decreased (P<0.05). However, there was no significant difference in the above indicators in the control group (P>0.05). According to the intra-group comparison before and after 14 days of treatment, there were significant differences in the above-mentioned ascites related indicators in both groups (P<0.05). According to the inter-group comparison after 7 days of treatment and 14 days of treatment, there were significant differences in 24-hour urine volume and ascites depth in supine position between the two groups (P<0.05). ②According to the intra-group comparison before and after 14 days of treatment, the serum creatinine level in the control group increased (P>0.05), but there was no significant difference in indicators of liver function, renal function, electrolyte, and coagulation function in the treatment group (P>0.05). According to the inter-group comparison after 14 days of treatment, there was no significant difference in the above indicators between the groups (P>0.05).ConclusionCompared with the single use of western medicine conventional therapy, the combined use of Tuduzi Buqi Decoction and western medicine is more conducive to the resolution of refractory ascites in liver cirrhosis without increasing the risk of renal function injury and electrolyte disturbance.关键词：liver cirrhosis;refractory ascites;Tuduzi Buqi Decoction;kidney function;electrolyte;clinical research
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发布时间：2022-08-03 - 摘要：ObjectiveTo explore the role of Yihefang in regulating the microecological structure of gut microbiota, bone marrow abnormal inflammation and serum lipid metabolism in mice by 16S rDNA sequencing technology.MethodsThirty male C57BL/6J mice were randomly divided into normal group, high-fat group and Yihefang group. The high-fat group was given a high-fat diet to induce a lipid metabolism disorder model, and the Yihefang group was given the same amount of Yihefang liquid on the basis of the high-fat group. At 12 weeks of the treatment, samples of fresh feces, serum and bone marrow fluid were collected from mice to detect serum cholesterol and triglyceride levels. qPCR and Western blot were used to detect IL-6 levels of inflammatory factors, and 16S rDNA sequencing to detect bacterial diversity of stool samples.ResultsCompared with the high-fat group, the body weight, serum cholesterol and triglyceride levels of mice in Yihefang group were significantly reduced (P<0.05, P<0.01); IL-6 transcription and protein expression in bone marrow were significantly reduced (P<0.05); and the microecological structure of gut microbiota tended to be normalization.ConclusionYihefang can regulate the disorder of lipid metabolism induced by high-fat diet, alleviate the abnormal inflammatory reaction of bone marrow, and also play a positive role in the recovery and reconstruction of intestinal microecological imbalance.关键词：lipid metabolism;high fat diet;model mice;gut microbiota;inflammation;16S rDNA sequencing;traditional Chinese medicine research
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发布时间：2022-08-03 - 摘要：ObjectiveTo explore the mechanism of Ziziphi Spinosae Semen extract affecting the mice growth in length and slow wave sleep of rats by regulating the cAMP-PKA pathway.Methods①Forty-eight Kunming male mice were randomly divided into 6 groups: blank control group (intragastrically administered with deionized water, and injected with sterilized 0.9% NaCl in the lateral ventricle after the last intragastric administration), blue ink group (intragastrically administered with deionized water, and injected with filtered and sterilized blue ink in the lateral ventricle after the last intragastric administration), medication group (intragastric administration of the Ziziphi Spinosae Semen extract, and injection of filtered and sterilized blue ink in the lateral ventricle after the last intragastric administration), positive control group (intragastric administration of jujube saponin A, and injection of filtered and sterilized blue ink in the lateral ventricle after the last intragastric administration), Gi/o protein inhibitor + medication group (intragastric administration of Ziziphi Spinosae Semen extract, and injection of PT with filtered and sterilized blue ink in the lateral ventricle after the last intragastric administration) and 5-HT1AR inhibitor + medication group (intragastric administration of Ziziphi Spinosae Semen extract, and injection of P-MPPF with filtered and sterilized blue ink in the lateral ventricle after the last intragastric administration). The mice in each group were given corresponding drugs by intragastric administration for 20 days. The body length of the mice was measured. The level of serum growth hormone (GH), the activity of 5-hydroxytryptamine 1A receptor (5-HT1AR) in brain tissue, the contents of PKA and Gi/o protein in brain tissue were measured by ELISA. The expression of 5-HT1AR protein in mouse brain tissue was determined by Western blot. ②Thirty six SD rats were randomly divided into 6 groups (6 rats in each group). The grouping and administration methods were the same as those of mice. The sleep phase differences of rats were analyzed by WXL sleep automatic analysis system after 3 days of continuous administration.Results①Compared with the blank control group and the blue ink group, the body length of mice increased significantly in the medication group, the Gi/o protein inhibitor + medication group, the 5-HT1AR inhibitor + medication group and the positive control group (P<0.01); Compared with the blank control group, the serum GH level, 5-HT1AR protein content and Gi/o protein content of mice in the medication group and the positive control group increased significantly (P<0.01), and the 5-HT1AR activity was also increased (P<0.05), while the PKA content was decreased significantly (P<0.01) ; Compared with the blue ink group, the serum GH level was significantly increased (P<0.01) and Gi/o protein content and 5-HT1AR activity were increased(P<0.05), while the PKA content was decreased significantly (P<0.01) in the medication group and the positive control group; Compared with the Gi/o protein inhibitor + medication group and the 5-HT1AR inhibitor + medication group, the serum GH level, the protein content of 5-HT1AR were increased significantly(P<0.01) and 5-HT1AR activity was increased (P<0.05) in the medication group and the positive control group. There were no significant differences in body length, GH level, 5-HT1AR activity, PKA content, Gi/oprotein content and 5-HT1AR protein content between the medication group and the positive control group (P>0.05). ②Compared with the blank control group, the total sleep period was increased significantly in the medication group (P<0.01) and the positive control group (P<0.05). Compared with the blue ink group, the total sleep period was increased significantly in the medication group (P<0.01) and the positive control group (P<0.05). Compared with the Gi/o protein inhibitor + medication group and the 5-HT1AR inhibitor + medication group, the total sleep period increased significantly (P<0.05) in the medication group and the positive control group. There was no significant difference in the total sleep period between the medication group and the positive control group (P>0.05).ConclusionZiziphi Spinosae Semen extract can increase the expression of Gi/o protein, increase the content and activity of 5-HT1AR in the brain through cAMP-PKA pathway, prolong slow wave sleep and promote the secretion of growth hormone to play the biological effect of promoting bone growth.关键词：Ziziphi Spinosae Semen extract;Jujube saponin A;bone growth;cAMP-PKA pathway;Gi/o protein;5-HT1A receptor;model mice;model rats;slow wave sleep
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发布时间：2022-08-03 - 摘要：ObjectiveTo establish a new method for determination of seven components (allantoin, hesperidin, narirutin, isosinensetin, nobiletin, 3, 5, 6, 7, 8, 3’, 4’-heptamethoxyflavone, tangeretin) in Jianpi Prescription by ultra performance liquid chromatography (UPLC) with diode array detector (DAD) and to carry out its application research.MethodsAgilent 1290 series ultra high performance liquid chromatography was used to detect the seven components by UV multi-wavelength detection. The detection wavelength of allantoin was set at 224 nm, with narirutin and hesperidin at 284 nm, isosinensetin at 342 nm, and nobiletin, 3, 5, 6, 7, 8, 3’, 4’-heptamethoxyflavone and tangeretin at 333 nm. The method was used to analyze the contents of seven components in Jianpi Prescription extracts prepared by different extraction methods.ResultsIn the appropriate concentration range for all above seven components, the concentrations showed quite good linear relationship with the peak area (r>0.999 3).All indexes of the methodological investigations including precision, repeatability, stability and accuracy met the requirements. The contents of seven components in Jianpi Prescription extracts prepared by various of extraction methods were significantly different. The concentration of allantoin in water decoction of Jianpi prescription was the highest, while the concentrations of hesperidin, narirutin, isosinensetin, nobiletin, 3, 5, 6, 7, 8, 3’, 4’-heptamethoxyflavone and tangeretin in ethanol reflux liquid were higher than those in water decoction of Jianpi Prescription. It was suggested that in the extraction and purification process of Jianpi Prescription, the rationality of the method should be evaluated from the point of view of multi-component content analysis.ConclusionThe established method based on UPLC-DAD in this study is stable and reliable, and can provide reference for its process research and quality control.关键词：Jianpi Prescription;UPLC-DAD;multi-component content analysis;hesperidin;allantoin
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发布时间：2022-08-03 - 摘要：This review summarizes the research progress on the drug resistance mechanisms of Candida albicans (CA) causing vulvovaginal candidiasis from 6 aspects, including drug target change, drug pump high expression, biofilm formation, vesicle formation, calcium signaling pathway and altered sterol introduction, in order to provide theoretical basis for further research on drug resistance mechanism of CA and guidance for rational use of antifungal drugs in clinical practice.关键词：Candida albicans;drug resistance;efflux pump;biofilms;review
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发布时间：2022-08-03 - 摘要：Abnormally increased hepatic gluconeogenesis plays an important role in diabetes. Insulin resistance is the pathological basis of T2DM, leading to an increase in hepatic gluconeogenesis. PEPCK and G6Pase, two key enzymes of hepatic gluconeogenesis, are activated by transcription factors such as CREB, TORC2 and PGC-1ɑ, which are also molecular targets of medicines in regulating blood glucose. From the perspective of traditional Chinese medicine, excessive hepatic gluconeogenesis is associated with dysfunction of the spleen in transport and storing essence. In recent years, "treatment from spleen" , by the regulation of spleen transport and storing essence function to treat diabetes of deficiency of Qi and Yin syndrome, spleen deficiency and dampness syndrome and damp-heat syndrome to reduce the hepatic gluconeogenesis, has become a hot spot of diabetes research. The anti-diabetic activity of traditional Chinese medicines has been proved and their mechanisms in improving insulin resistance and inhibiting transcription of hepatic gluconeogenesis have been identified, through treating diabetes from spleen, replenishing vital energy, dispelling dampness and clearing heat and removing dampness. These researches holds extensive promise for the clinical application of traditional Chinese medicine.关键词：diabetes;traditional Chinese herbal formula;gluconeogenesis;insulin resistance;treatment from the spleen;review
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发布时间：2022-08-03
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