最新刊期
期 10 ， 2021
- 摘要：The theory of "hidden toxicity due to vital qi deficiency" as the core pathogenesis of cancer metastasis in the subclinical stage has been proposed and verified. In the process of cancer metastasis, there is a struggle between "vital qi" and "hidden toxicity" . "If vital qi prevails, the hidden toxicity will be subdued without causing diseases; however, if vital qi is weak, the hidden toxicity will burst out to cause diseases" . The essence actually is the fight between cancer cells and the host’s cancer suppression power. After the radical resection of solid tumors, the residual cancer cells (e.g., circulating tumor cells, disseminated tumor cells, dormant tumor cells, etc.) are latent in the body, being in the dormant or quiescent phase. When there is a deficiency of vital qi (i.e., when the immune surveillance and immune clearance efficiency decreases), cancer cells will enter the proliferative phase from the quiescent phase, leading to the occurrence of clinical metastasis. Therefore, the key strategy to prevent the occurrence of cancer metastasis is to comprehensively regulate the patient’s vital qi in terms of form, qi and spirit.关键词：cancer metastasis;lung cancer;subclinical stage;hidden toxicity due to vital qi deficiency;traditional Chinese medicine pathogenesis;circulating tumor cells;immune escape
- 19
- |
- 607
- |
- 0
发布时间：2022-08-03 - 摘要：ObjectiveTo analyze the research status and hotspots of traditional Chinese medicine (TCM) treatment of osteosarcoma and to reveal the dynamic changes and development trend of research hotspots for the purpose of providing ideas and basis for the research of TCM treatment of osteosarcoma.MethodsThe research literature on TCM treatment of osteosarcoma in databases such as China National Knowledge Infrastructure (CNKI), Wanfang Database of China Medical Collections, and Chinese biomedical literature service system (SinoMed) was retieved from the date of database’s establishment to June 2019. Bibliometric methods were adopted to analyze and describe the basic information of the research literature (publication time, issuing institution, source of publication, fund projects, etc.). The Citespace software was used to depict and visualize the knowledge map of research hotspots, including co-occurrence analysis, cluster analysis, timeline view analysis and emergence analysis of literature keywords.Results①After screening, 197 valid articles were finally obtained, among which basic research articles were more than clinical research articles. ②The number of articles related to TCM treatment of osteosarcoma from 1988 to 2019 showed an overall upward trend, with a slow and fluctuating rise. ③Fujian University of Traditional Chinese Medicine (the main campus and affiliated institutions) published the most articles on TCM treatment of osteosarcoma. Generally speaking, there was relatively little cooperation among different regions and different universities and institutions. ④The relevant articles were published in 38 different sources, including journal articles and dissertations respectively, and the sources of publication were relatively scattered. ⑤The National Natural Science Foundation of China (NSFC) has topped the sponsorship, but less than 40% of the articles were funded. ⑥The author (first author) with the highest number of publications was Chen Yongqiang from the Shanghai Municipal Hospital of Traditional Chinese Medicine affiliated to Shanghai University of Traditional Chinese Medicine; the number of related scholars was small and they were scattered, and there was relatively little collaboration among different authors from different regions. ⑦The co-occurrence analysis and cluster analysis showed that the research hotspots of TCM treatment of osteosarcoma mainly focused on how to regulate the proliferation and apoptosis of osteosarcoma cells, and curcumin, Pien Tze Huang, and melittin were the most extensively and deeply studied. ⑧The timeline view analysis showed that the hot keyword change of TCM treatment of osteosarcoma from 2003 to 2019 was significant, and immune regulation may become the frontier research point. ⑨The emergence analysis showed that tripterine had the highest emergence rate, which surged from 2015 onwards.ConclusionsThe research on TCM treatment of osteosarcoma has achieved certain progress, but the overall research is still in the primary stage, and the academic cooperation among different regions and different research teams needs to be strengthened. At present, the research mainstream of TCM intervention in osteosarcoma is the research on TCM monomers, while the research on TCM syndrome standardization of osteosarcoma may be one of the key research directions in the future.关键词：osteosarcoma;traditional Chinese medicine treatment;traditional Chinese medicine monomers;research hotspot;research status;bibliometrics;visualization technique
- 30
- |
- 521
- |
- 0
发布时间：2022-08-03 - 摘要：ObjectiveTo investigate the distribution and clinical features of TCM syndromes in bladder cancer patients in Shanghai.Methods525 cases of bladder cancer in Shanghai were investigated by TCM syndrome questionnaire, and the pathological results (yes/non-invasive bladder cancer) were investigated. Then the pathological cell grade (high/low risk), cystoscopy (number of tumors: single/multiple), history (age of onset, incidence: initial/recurrence) were investigated in the non-invasive bladder cancer patients and life scale was used to evaluate the quality of life.Results①Among 525 patients, male patients accounted for 68.0% and female patients accounted for 32.0%. In the patients’ TCM syndrome, the damp-heat perfusion type and stasis toxin accumulation type were relatively high, Then followed by deficiency of kidney yin type, non-documented/other TCM syndrome type and deficiency of spleen and kidney yang; Pathology showed that there was a difference in TCM syndrome distribution between non-invasive bladder cancer and invasive bladder cancer (P<0.05). The highest proportion of TCM syndrome type was damp-heat perfusion type in non-invasive bladder cancer. The most invasive bladder cancer patients was stasis toxin accumulation type. ②The most middle and low grade TCM syndrome in non-invasive bladder cancer patients was damp-heat perfusion type. The high level was stasis toxin accumulation type. ③Among the main TCM syndromes of non-invasive bladder cancer, the scores of urination symptom and intestinal symptom of damp-heat perfusion type were significantly higher than those of other syndromes (P<0.05), and the score of pain symptom of stasis toxin accumulation type was significantly higher than that of other syndromes (P<0.05).ConclusionsThe TCM syndromes of bladder cancer in Shanghai are mainly damp-heat perfusion type and stasis toxin accumulation type, among which non-invasive bladder cancer had the most incidence. The distribution of TCM syndromes of non-invasive bladder cancer was different from that of invasive bladder cancer. The non-invasive bladder cancer patients with stasis toxin accumulation type were significantly different from other syndrome types in terms of pathological grade, infiltration and recurrence rate, which should be paid attention in clinical.关键词：bladder cancer;non-invasive;TCM syndromes;epidemiology;quality of life;stasis toxin accumulation
- 57
- |
- 261
- |
- 0
发布时间：2022-08-03 - 摘要：ObjectiveTo analyze and summarize Professor Liu Sheng’s medication principles in the treatment of triple negative breast cancer (TNBC) based on data mining technology.MethodsThe medical records and prescriptions for the treatment of TNBC from Professor Liu Sheng’s outpatient clinic were collected to establish a database. Excel 2010 was used for data mining and clustering, and statistical information on the use frequency and efficacy, four qi, five flavors, and meridian tropism of traditional Chinese herbal medicines (TCHMs) was obtained. IBM SPSS Modeler 14.1 software was used for modeling, and medicinal association rule analysis and symptom-medicine association rule analysis were performed with Apriori algorithm. IBM SPSS 21.0 statistical software was used for cluster analysis to obtain core medications.Results①A total of 66 patients were included, involving 1,000 prescriptions and 252 TCHMs. ②The more frequently used TCHMs were those that enter the liver, spleen, lung, stomach, and kidney meridians. TCHMs with a use frequency over 50% were Atractylodis Macrocephalae Rhizoma, Poria, Salvia chinensis Benth, Codonopsis Radix, Curcumae Rhizoma, Solanum nigrum L., Epimedii Folium, Scutellariae Barbatae Herba, Polygoni Multiflori Caulis, Albiziae Cortex, and Lilii Bulbus. ③According to the efficacy classification of TCHMs, the top 6 in order of use frequency were heat-clearing and detoxifying medicinals, qi-tonifying medicinals, yang-tonifying medicinals, yin-tonifying medicinals, heart-nourishing and mind-tranquilizing medicinals, and blood-activating and mass-removing medicinals. ④The applied TCHMs were mostly cold in property and sweet, bitter and pungent in flavor. ⑤The medicinal association rules showed: the more frequently used medicinal pairs were Albiziae Cortex-Polygoni Multiflori Caulis, Lilii Bulbus-Polygoni Multiflori Caulis, Lilii Bulbus-Albiziae Cortex, and Anemarrhenae Rhizoma-Lilii Bulbus; the more frequently used medicinal groups of 3 TCHMs were Albiziae Cortex-Polygoni Multiflori Caulis-Lilii Bulbus, and Lilii Bulbus-Polygoni Multiflori Caulis-Anemarrhenae Rhizoma; the more frequently used medicinal groups of 4 TCHMs were Lilii Bulbus-Albiziae Cortex-Polygoni Multiflori Caulis-Anemarrhenae Rhizoma. ⑥A total of 14 medicinal categories were obtained by cluster analysis. Combined with the results of frequency analysis, the core TCHMs used by Professor Liu Sheng for the treatment of TNBC were Atractylodis Macrocephalae Rhizoma, Poria, Salvia chinensis Benth, Codonopsis Radix, Curcumae Rhizoma, Solanum nigrum L. and Epimedii Folium. The results of association rules showed that the common medicinal pairs used by Professor Liu Sheng for the treatment of concurrent symptoms of TNBC were Polygoni Multiflori Caulis-Albiziae Cortex, Lilii Bulbus-Anemarrhenae Rhizoma, Atractylodis Rhizoma-Magnoliae Officinalis Cortex, and Pinelliae Rhizoma-Citri Reticulatae Pericarpium.ConclusionProfessor Liu Sheng treats TNBC by strengthening the spleen and supplementing kidney, resolving phlegm and eliminating blood stasis, removing toxins and extinguishing wind, and he treats both primary and concurrent syndromes simultaneously.关键词：triple negative breast cancer;data mining;researoh of traditinal Chinese herbal medicine;Liu Sheng
- 12
- |
- 301
- |
- 0
发布时间：2022-08-03 - 摘要：The article summarized professor Li Yan’s clinical experience in treating bladder cancer by elimination of pathogens through purgation and diuresis. The occurrence of bladder cancer was due to the insufficiency of the spleen and kidney, while dampness in Sanjiao was an important pathological mechanism. Therefore, tonifying deficiency was mainly to strengthen the spleen and kidney, while the way to eliminating dampness pathogenic factors was purgation and diuresis, adjusting qi activity of Sanjiao. Giving consideration on both strengthening the body resistance and eliminating pathogenic factors could improve the clinical efficacy, and one proven case was presented.关键词：bladder cancer;traditional Chinese medicine therapy;elimination of pathogens through purgation and diuresis;expert experience;Li Yan
- 19
- |
- 383
- |
- 0
发布时间：2022-08-03 - 摘要：The clinical application of programmed death-1(PD-1) has brought remarkable progress in the treatment of non-small cell lung cancer (NSCLC), but it may cause immunotherapy-related adverse reactions and nearly half of the patients still can hardly benefit from it. According to the clinical manifestations at different treatment stages of NSCLC treated with PD-1 monoclonal antibody, four syndromes can be identified among NSCLS patients: wind-heat attacking the lung syndrome, spleen-deficiency and phlegm-dampness syndrome, qi stagnation and blood stasis syndrome, and dual deficiency of qi and yin syndrome. It has been proved that the appropriate application of Sangju Decoction, Shenling Baizhu Powder, Xuefu Zhuyu Decoction, Shengmai Decoction plus Shashen Maidong Decoction to corresponding syndromes can effectively improve the clinical symptoms of patients and reduce adverse reactions related to immunotherapy. One proved case is attached.关键词：non-small cell lung cancer;programmed death-1 (PD-1);immunotherapy;traditional Chinese medicine therapy
- 10
- |
- 489
- |
- 0
发布时间：2022-08-03 - 摘要：The purpose of this paper is to explore Professor Luo Xuexin’s clinical experience of using the method of mild regulation of yin and yang in the treatment of peri-menopausal syndrome after endocrine therapy of breast cancer. It is considered that its pathogenesis is kidney deficiency and imbalance between yin and yang, which is treated with modified Baihe Dihuang Decoction and Ganmai Dazao Decoction based on syndrome differentiation by the methods of mildly regulating yin and yang, tonifying the kidney and soothing the liver, nourishing the heart to tranquilize spirit. And one proven case is presented.关键词：breast cancer;peri-menopausal syndrome;mild regulation of yin and yang;therapy of traditional Chinese medicine;Luo Xuexin
- 16
- |
- 244
- |
- 0
发布时间：2022-08-03 - 摘要：This paper introduced professor Qian Lin’s experience in treating human papilloma virus (HPV) persistent infection. Professor Qian considered that the deficiency of qi and yin, and dampness, heat, toxicity in lower jiao were the main pathogenesis of HPV persistent infection. He followed the treatment principle of enriching qi and yin, removing heat and toxins, and combined internal and external treatment to treat this disease. The internal treatment was to regulate the body’s immunity by enriching qi and yin, and the external treatment was mainly to remove heat and toxins to resist virus. Besides, he attached importance to the treatment from dampness and took advantage of the multiple effects of herbal medicines to remove dampness throughout the entire treatment process.关键词：human papilloma virus;persistent infection;the internal and external treatment;enriching qi and clearing heat;expert’s experience;Qian Lin
- 9
- |
- 218
- |
- 0
发布时间：2022-08-03 - 摘要：The purpose of this paper is to discuss the meaning of "Keqi(pathogenic factors)" in Treatise on Exogenous Febrile Diseases and the guiding effect of its thought on clinic. Starting with the discrimination of syndrome of binding in chest and syndrome of stuffiness, it is known that "syndrome of binding in chest" is easy to occur in yang meridian and "syndrome of stuffiness" in yin meridian, and it is concluded that "emptiness in stomach" is not equal to "stomach qi deficiency" . Furthermore, it is known that the pathogenesis of "Keqi" in the corresponding clauses of Treatise on Exogenous Febrile disease is also different. The Keqi in " Keqi invading the diaphragm" refers to the pathogenic heat sinking inside of the superficial yang, while the Keqi in "ascending counterflow of Keqi" refers to stomach qi deficiency and effulgent heart fire.关键词：Treatise on Exogenous Febrile Diseases;Keqi;syndrome of binding in chest;syndrome of stuffiness
- 9
- |
- 252
- |
- 0
发布时间：2022-08-03 - 摘要：ObjectiveTo observe the clinical efficacy of internal and external application of Wenyang Sanjie Decoction combined with conventional therapy in the treatment of tumor osseous metastasis pain.MethodsNinety-three patients with tumor osseous metastasis pain were randomly divided into a control group (n=47) and a treatment group (n=46). The control group was administered with conventional analgesic therapy, while the treatment group was administed with internal and external application of Wenyang Sanjie Decoction besides the treatment measures of the control group. Both groups were treated continuously for 6 cycles. The frequency of pain outbreaks in the two groups was observed, the changes of pain numeric rating scale (NRS) score, 24-h dosage of oxycodone hydrochloride, laboratory indexes, and the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire-Bone Metastases22 (EORTC QLQ-BM22) score were compared, and safety evaluation was also performed.Results①During the trial, there was one drop-out case in the treatment group, and in the control group one case dropped out and one case was removed. Finally, 90 cases completed the trial, with 45 cases in each group. ②The NRS score decreased in both groups after 2 cycles of treatment, 4 cycles of treatment and 6 cycles of treatment respectively compared with that before treatment (P<0.001); the NRS score of the treatment group was lower than that of the control group after 2 cycles of treatment, 4 cycles of treatment, and 6 cycles of treatment respectively (P<0.05). ③During the whole treatment process, the frequency of painful outbreaks in the treatment group was lower than that in the control group, and the difference was statistically significant (P<0.05). ④Compared with the condition after 3 days of treatment, the 24-h dosage of oxycodone hydrochloride decreased in the treatment group (P<0.001) but increased in the control group (P<0.001) after 4 cycles of treatment and 6 cycles of treatment; the 24-h dosage of oxycodone hydrochloride in the treatment group was lower than that in the control group after 4 cycles of treatment and 6 cycles of treatment (P<0.05). ⑤Compared with the condition before treatment, the serum calcium level was lowered in the treatment group after 4 cycles of treatment (P<0.01); after 6 cycles of treatment the tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels was lowered in the treatment group (P<0.01), while the alkaline phosphatase (ALP) level increased in the control group (P<0.01). The IL-6 level in the treatment group was lower than that in the control group after 4 cycles of treatment and 6 cycles of treatment respectively (P<0.05). ⑥Compared with the pre-treatment condition, the scores of pain site, pain degree and dysfunction modules in the EORTC QLQ-BM22 were lowered in the treatment group after 2 cycles of treatment (P<0.01). The intra-group comparison between the condition after 4 cycles of treatment and that after 6 cycles of treatment showed that the scores of pain site, pain degree and dysfunction modules decreased in both groups (P<0.01). After 6 cycles of treatment, the score of dysfunction module in treatment group was lower than that in the control group (P<0.05). ⑦The incidence of adverse reactions in the treatment group was lower than that in the control group, and the difference was statistically significant (P<0.05).ConclusionsCompared with the single use of conventional analgesia, the additional use of internal and external application of Wenyang Sanjie Decoction in the treatment of tumor osseous metastatic pain has achieved a satisfactory result, which could reduce the frequency of pain outbreaks and the dosage of analgesic drugs, and improve the survival quality of patients. The mechanism may be related to the reduction of serum TNF-α, IL-6 and calcium levels.关键词：tumor osseous metastases;cancer pain;integrated traditional Chinese and Western medicine therapy;Wenyang Sanjie;internal use of traditional Chinese herbal medicine;external application of traditional Chinese herbal medicine;cytokine
- 15
- |
- 330
- |
- 0
发布时间：2022-08-03 - 摘要：ObjectiveTo establish a sensitive and rapid high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) method to investigate the pharmacokinetics of antitumor components (Euphorbia factor L2, L3) after intragastric administration, intraperitoneal injection and intravenous injection in rats.MethodsThe plasma concentrations of Euphorbia factors L2 and L3 were determined by LC-MS /MS internal standard method. The mobile phase was methanol-water (80: 20). The chromatographic column was BSD Hypersil C18 (100 mm × 2.1 mm, 3 μm). The column temperature was 30 ℃, the flow rate was 0.2 mL/min, and the injection volume was 5 μL. Mass spectrometry conditions: electrospray ionization (ESI) source, positive ion mode scanning, multi response monitoring mode (MRM) were used to detect the effective components, and pharmacokinetic parameters were calculated by WinNonlin 4.1 pharmacokinetics software.ResultsThe good linear range of Euphorbia factors L2 and L3 was 5~1 000 ng/mL (r2 > 0.999). The extraction recoveries ranged from 90.5% to 107.2%. The matrix effects ranged from 89.1% to 94.2% and from 88.1% to 93.9%, respectively. The intra-day and inter-day precisions (RSDs, %) were below 12.8%. Cmax and AUC0-24 h after intravenous injection of Euphorbia factor L2 and L3 were (20.76±7.55), (13.48±4.59) μg/mL and (8.43±2.34), (5.13±1.38) h·μg-1·mL-1, respectively; Cmax and AUC0-24 h after intraperitoneal injection of Euphorbia factor L2 and L3 were (0.95 ±0.26), (0.27±0.06) μg/mL and (3.52±0.90), (0.98±0.29) h·μg-1·mL-1, respectively. After intragastric administration of Euphorbia factors L2 and L3, the concentrations of several samples were lower than the detection limit and could not be detected.ConclusionThe method is sensitive, rapid and selective, and can be used for the simultaneous determination of Euphorbia factors L2 and L3 in rat plasma and its pharmacokinetics. It showed that the bioavailability of Euphorbia factors L2 and L3 were low after intragastric administration, and the exposure dose of Euphorbia factor L2 was higher than that of L3 in rats after intraperitoneal injection.关键词：Euphorbia factor L2;Euphorbia factor L3;LC-MS/MS;pharmacokinetics antineoplastic;traditional Chinese herbal medicine research
- 11
- |
- 752
- |
- 0
发布时间：2022-08-03 - 摘要：ObjectiveTo study the effects of Oldenlandia diffusa roxb and Scutellariae barbatae herba (Od-Sb) on the proliferation and apoptosis of HepG2/ADM.Methods①In vitro experiment: the effect of different concentrations of Od-Sb on the survival rate of HepG2/ADM was detected by CCK8 assay; the effect of Od-Sb on HepG2/ADM clonal formation ability was detected by cloning formation experiment; the effect of Od-Sb on the apoptosis of HepG2/ADM was detected by flow cytometry; the effect of Od-Sb on the protein expression of Ki-67 and Bcl-2 was detected by Western blot. ②In vivo experiment: the subcutaneous transplanted tumor of HepG2/ADM in nude mice was used as a model to observe the inhibitory effect of Od-Sb on the tumor, and the proteins expression of Ki-67 and Bcl-2 was detected by immunohistochemistry.Results①In vitro experiments, it was showed that compared with the control group, Od-Sb could inhibit the proliferation of HepG2/ADM cells, inhibit the formation of HepG2/ADM cells, promote its apoptosis, and down-regulated the expression of Ki-67 and Bcl-2 proteins (P<0.05) . ② In vivo experiments showed that compared with the control group, Od-Sb inhibited the growth of HepG2/ADM subcutaneous transplanted tumor body, and down-regulated the expression of Ki-67 and Bcl-2 proteins (P<0.05, P<0.001) .ConclusionOd-Sb can down-regulate the expression of Ki-67 and Bcl-2 proteins, inhibit the growth of HepG2/ADM and promote its apoptosis. Od-Sb can partially reverse the resistance of HepG2/ADM through the mechanism of proliferation-apoptosis regulation.关键词：Oldenlandia diffusa roxb;Scutellariae barbata herba;hepatocellular carcinoma;adrimycin;drug resistance;model mice;traditional Chinese herbal medicine research
- 13
- |
- 472
- |
- 0
发布时间：2022-08-03 - 摘要：ObjectiveTo qualitatively analyze the chemical composition of Qinsu Capsule (QSC) by Ultra performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS) and mass spectrometry database, and establish HPLC fingerprint for QSC.MethodsThe Agilent ZORBAX RRHD SB-C18(2.1 mm×100 mm, 1.8 μm) was used with a mobile phase of acetonitrile-0.1% methanoic acid in gradient elution. The flow rate was 0.3 mL/min, the column temperature 25 ℃, and the detection wavelength 270 nm. UPLC-Q-TOF/MS was used for qualitative analysis in the positive and negative ion modes. The chromatographic fingerprints analysis of 10 batches of QSC were carried out in the Waters Xbridge Shield RP18(4.6 mm×250 mm, 5 μm) column with a mobile phase of acetonitrile-0.05% phosphoric acid in gradient elution, the flow rate 0.8 mL/min, the column temperature 30 ℃, and the detection wavelength 270 nm.ResultsTotally 68 chemical components were detected from QSC, and 22 of them were identified. The HPLC fingerprint for 10 batches of QSC was established and 33 common peaks were obtained. The similarity of 10 batches of QSC was over 0.990.ConclusionThe chemical composition of QSC was qualitatively analyzed and HPLC fingerprint was established, which can provide support for the further study of substance basis and quality standard promotion of QSC.关键词：Qinsu Capsule;UPLC-Q-TOF/MS;fingerprint;high resolution mass spectrum database;traditional Chinese herbal medicine
- 10
- |
- 425
- |
- 0
发布时间：2022-08-03 - 摘要：ObjectiveTo establish a method for qualitative identification and quality control of Alismatis Rhizoma, Salviae miltiorrhizae Radix et Rhizoma, Poria and Cynomorii Herba in Shenkangning Capsule by thin layer chromatography (TLC) .MethodsThe single-factor test method was used to systematically investigate the factors of TLC conditions, including different developing solvents, chromogenic reagents, spot volume, temperature, humidity, and to determine the optimal TLC conditions for the identification of Alismatis Rhizoma, Salviae miltiorrhizae Radix et Rhizoma, Poria and Cynomorii Herba.ResultsThe TLC conditions for Alismatis Rhizoma and Salviae miltiorrhizae Radix et Rhizoma were to develop on silica gel G thin-layer plate with n-hexane-ethyl acetate-formic acid (7∶4∶0.6) as the developing solvent. After the plate was taken out and dried, the fluorescent spots were observed under ultraviolet lamp (365 nm) , then sprayed with 2% phosphomolybdate ethanol solution, heated at 105℃ until the spots became clear. The TLC conditions for Poria were to develop on silica gel G thin-layer plate with n-hexane-ethyl acetate-formic acid (7∶4∶0.4) as the developing solvent. After the plate was taken out and dried, the fluorescent spots were observed under ultraviolet lamp (365 nm) . The TLC conditions for Cynomorii Herba were to develop on silica gel G thin-layer plate with cyclohexane-ethyl acetate-methanol-formic acid (8∶6∶1∶1) as the developing solvent After the plate was taken out and dried, it was soaked in 0.04% DPPH absolute ethanol solution. The spots were clear under daylight after the plate was dried again.ConclusionsThin layer chromatography features clear identification spots, strong specificity, negative control without interference. The method is also simple, convenient and has good repeatability, which can be adopted for the quality control of Shenkangning capsule.关键词：Shenkangning Capsule;thin layer chromatography;Alismatis Rhizoma;Salviae miltiorrhizae Radix et Rhizoma;Poria;Cynomorii Herba
- 16
- |
- 867
- |
- 0
发布时间：2022-08-03 - 摘要：ObjectiveTo investigate the effect of Astragaloside Ⅳ on microglia activity in Alzheimer’s disease (AD) rats based on mitogen activated protein kinase 5 (MEK5)/extracellular signal regulated kinase 5 (ERK5) signaling pathway.MethodsSixty clean grade healthy SD male rats were selected, and 10 were randomly selected as a normal group. The remaining 50 were randomly divided into the model group, the positive control group, the astragaloside Ⅳ low dose group, the Astragaloside Ⅳ medium dose group and the Astragaloside Ⅳ high dose group after the establishment of the AD model, with 10 rats in each group. The positive control group was intervened with huperzine A (0.02 mg/kg), the normal group and model group were administered with the equal volume of 0.9% NaCl solution, and the astragaloside Ⅳ low dose group, the Astragaloside Ⅳ medium dose group and the astragaloside Ⅳ high dose group were administered with 15 mg/kg, 30 mg/kg and 60 mg/kg of Astragaloside Ⅳ, respectively. All groups were intervened for 21 days. Interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) levels as well as the expressions of glucose-regulated protein 78 (GRP78), C/EBP homologous protein (CHOP), MEK5 and ERK5 were tested.ResultsCompared with normal group, the levels of IL-1, TNF-α and the expressions of GRP78, CHOP, MEK5 and ERK5 in model group, positive control group, low dose astragaloside Ⅳ group, medium dose astragaloside Ⅳ group and high dose astragaloside Ⅳ group were increased (P<0.05). Compared with model group, the levels of IL-1, TNF-α levels and the expressions of GRP78, CHOP, MEK5 and ERK5 in positive control group, low dose astragaloside Ⅳ group, medium dose astragaloside Ⅳ group and high dose astragaloside Ⅳ group were decreased in a dose-dependent manner, and also decreased with dose increase (P<0.05).ConclusionAstragaloside Ⅳ can inhibit the activity of microglia and reduce the apoptosis of neurons in AD rats based on MEK5 /ERK5 signaling pathway.关键词：astragaloside Ⅳ;mitogen activated protein kinase 5;extracellular signal regulated kinase 5;Alzheimer’s disease;microglia;model rat;research of traditional Chinese herbal medicine
- 17
- |
- 544
- |
- 0
发布时间：2022-08-03 - 摘要：The research progress on the effect of oxidative stress in thyroid cancer and intervention of Chinese medicine monomer in vitro were reviewed. Oxidative stress affected the process of thyroid cancer in a paradoxical way, it not only participated in the formation and development of tumors, but also led to the death of cancer cells; the accumulation of reactive oxygen species was involved in the occurrence and progression of thyroid cancer, which could also lead to the migration and invasion of cancer cells. There were some Chinese medicine monomers such as parthenolide, icariin, triptolide, crocin, artesunate, curcumin, salvianolic acid B, puerarin and shikonin could cause the accumulation of oxidative substances in thyroid cancer cells in vitro, reduce the production of antioxidant enzymes, thereby inducing the apoptosis of thyroid cancer cells, etc., so as to exert its therapeutic effect.关键词：oxidative stress;thyroid cancer;cell apoptosis;Chinese medicine monomer;review
- 10
- |
- 463
- |
- 0
发布时间：2022-08-03 - 摘要：Sophorae Flavescentis Radix is a commonly used Chinese medicine for clearing heat and drying dampness, which is mainly used for the treatment of damp-heat syndrome by oral administration, or for skin and gynecological diseases by external application. Sophorae Flavescentis Radix contains a variety of chemical constituents, and some new chemical constituents have been isolated and discovered in recent years. The purpose of this paper is to summarize comprehensively the chemical constituents from Sophorae Flavescentis Radix by searching major scientific databases (CNKI, Web of Science, PubMed, SciFinder, Sci-hub, i-Data, Microsoft Academic, and Research Gate) for information published since 1953, and the bioactivities of some chemical constituents were highlighted in the paper. The chemical constituents from Sophorae Flavescentis Radix consist of alkaloids, flavonoids, phenylpropanoids, dibenzoyl derivatives, terpenoids, steroids, organic acids, fatty acids, and volatile oils, among which alkaloids and flavonoids are the principal chemical types, showing huge research value in antibacterial, anti-inflammatory, anti-virus and anti-tumor.关键词：Sophorae Flavescentis Radix;chemical constituents;bioactivity;pharmacologic action;research advance;review
- 29
- |
- 2718
- |
- 0
发布时间：2022-08-03
- Technical support is provided by Beijing Founder electronics co., LTD 沪ICP备13044091号
沪公网安备 31011502015228号 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
- Total Visits：70575 Visits Today：56
0