最新刊期
期 7 ， 2020
- 摘要：The cold and warm nature of ancient epidemic diseases was differentiated from their names. Clinically, the treatment of cold and warm epidemic diseases should be divided into three categories, namely, warming method, clearing method and cooling-warming combination method. For the coexistence of Shanghan and Wenbing in the clinic, we should trace back the origin of epidemic diseases, think about how to understand the inter-relations between Shanghan and Wenbing in the development process of epidemic diseases and take them as a whole to facilitate the study of traditional Chinese medicine and clinical practice.关键词：epidemic disease;differentiate cold and warm nature;divide treatment methods into three categories;holistic understanding of Shanghan and Wenbing
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发布时间：2022-08-31 - 摘要：Through sorting and analyzing of the main literatures of previous dynasties, this article differentiated COVID-19 in the categorization of Wenbing and Shanghan from aspects of solar terms of the outbreak, climate changes, clinical manifestations and reverse proofs on efficacy of the formulas, etc. It drew a conclusion that COVID-19 belonged to Shanghan, rather than Wenbing. COVID-19 started with Shanghan, and then developed into miscellaneous diseases, and simultaneously changed into complicated and deteriorated diseases due to incorrect treatments, so COVID-19 should be treated with methods of Shanghan.关键词：COVID-19;SARS-CoV-2;Shanghan;Wenbing;miscellaneous diseases;complicated and deteriorated diseases
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发布时间：2022-08-31 - 摘要：Since the outbreak of the pandemic, there has been a rapid increase in clinical researches of coronavirus disease 2019 (COVID-19). As a major public health event, the related research work has higher requirements for ethical review. We expounded the challenges faced by the ethical review work under pandemic, and also proposed countermeasures and suggestions by learning from the practical experience of Shuguang Hospital of Shanghai University of Traditional Chinese Medicine for the purpose of ensuring the health and safety of subjects and researchers during the special periods as well as the standardized and orderly development of clinical research.关键词：COVID-19;SARS-CoV-2;clinical research;ethical review
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发布时间：2022-08-31 - 摘要：Objective:To explore the clinical value and molecular mechanism of the core formula for novel coronavirus’s pneumonia (COVID-19) based on data mining and network pharmacology. MethodsWith the help of the Cloud Platform of Ancient and Modern Medical Records (V2.2.1), the published prescriptions of Chinese herbal medicines for COVID-19 were recorded, the statistics of syndrome patterns and the analysis of four qi and five flavors of traditional Chinese medicine were carried out, the rules of medication were excavated, and thus the core formula was preliminarily obtained. Then the active components and action targets of the core formula were searched by Traditional Chinese Medicine System Pharmacology Analysis Platform (TCMSP). The disease targets were screened by GeneCards and other databases, and the active component-target network and PPI protein interaction network were constructed by Cytoscape 3.7.2 software.After GO function analysis and KEGG pathway enrichment analysis of the target through DAVID database, the core formula was further refined and simplified. Finally, Autodockvina was used for molecular docking, preliminary screening and further verification of the core chemical composition set of Chinese herbal medicines used in core cases bound to the key target of COVID-19. Results:①A total of 38 clinical cases were obtained, and 77 prescriptions were collected, involving 164 Chinese herbal medicines. The four qi of the herbs are mostly cold, warm and mild, and the five flavors of the herbs are mostly bitter, pungent and sweet, which mainly belong to lung, stomach and spleen channels. Fourteen core formulae had obtained from 77 prescriptions, which include Houpu(Cortex Magnoliae Officinalis), Caodoukou(Semen Alpiniae Katsumadai), Gancao(Radix Glycyrrhizae), Jiegeng(Radix Platycodi), Chaihu(Radix Bupleuri), Kuxingren(Semen Armeniacae Amarum), Huangqin(Radix Scutellariae), Chenpi(Pericarpium Citri Reticula), Fuling(Poria), Lianqiao(Fructus Forsythiae), Banxia(Rhizoma Pinelliae), Guanghuoxiang(Herba Pogostemonis). ②There are 87 active components in the core formula, which can act on covid-19 through 56 potential targets. ③A total of 1 522 bioprocess entries and 161 KEGG pathways in GO were obtained by enrichment analysis(P<0.05). As a result, the formula was simplified and the herbs were retained such as Caodoukou(Semen Alpiniae Katsumadai), Chaihu(Radix Bupleuri), Guanghuoxiang(Herba Pogostemonis), Lianqiao(Fructus Forsythiae), Jiegeng(Radix Platycodi), Huangqin(Radix Scutellariae), Chenpi(Pericarpium Citri Reticula)as the final core formula. It involves anti-inflammatory, anti-oxidation, anti-fibrosis, inhibition of apoptosis, inhibition of virus replication and other processes. Conclusion:The core formula for the clinical cases of COVID-19 belongs to the combination of cold, warm and mild in nature, mainly acting in lung, stomach and spleen channels. It mainly regulates key targets such as IL-6, MAPK1, MAPK8, CASP3, IL-1B, RELA, MAPK14, MAPK3, PTGS2, CCL2, FOS and regulates signal pathways such as MAPK, NFkB, TNF, IL-17 to treat COVID-19.关键词：SARS-CoV-2;COVID-19;Chinese medicine;network pharmacology;molecular mechanism;multiple targets;cloud platform of ancient and modern medical records (V2.2.1)
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发布时间：2022-08-31 - 摘要：Through literature analysis, we organized the application of moxibustion in the prevention and treatment of “seasonal epidemic febrile diseases” and heat syndromes. According to the characteristics of the coronavirus disease 2019 (COVID-19), we believe that moxibustion can play a preventive and therapeutic role owning to its disinfection, antipyretic, anti-inflammatory, anti-virus and immune response regulation effects. Fumigation moxibustion and gentle moxibustion can also be adopted for mild and medium cases and convalescent patients.关键词：COVID-19;SARS-CoV-2;traditional Chinese medicine therapy;moxibustion;seasonal epidemic disease;prevention and treatment
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发布时间：2022-08-31 - 摘要：Objective:To retrospectively analyze the clinical efficacy and liver injury of 100 cases of coronavirus disease 2019 (hereinafter referred to as COVID-19) treated by integrated traditional Chinese and western medicine in Shanghai. MethodsWe collected the clinical data and treatment plan of 100 cases of COVID-19 admitted to Shanghai Public Health Clinical Center from January 22,2020 to February 10,2020, observed the tongue image, pulse condition, TCM syndromes, liver injury indexes, clinical outcome and prognosis, and compared the changes of clinical symptoms, inflammation and immune related indexes. Results:①In this study, COVID-19 cases presented 3 main traditional Chinese medicine (TCM) syndromes:heat toxin blocking the lung, damp toxin stagnating the lung and qi deficiency of the lung and spleen. There were more cases of excess syndrome than cases of deficiency syndrome, and cases of the lung-spleen qi deficiency syndrome were more than cases of the dual deficiency of qi and yin syndrome. ② There were 43 asymptomatic cases (43.0%) at the time of admission. After taking TCM decoction, the main symptoms of 52 symptomatic cases, such as fever, cough, expectoration, poor appetite and diarrhea, were improved to varying degrees. ③There were significant differences in serum leukocyte count (WBC), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and immune index (CD4+) before and after 1 week of TCM decoction treatment (P<0.05). ④A total of 16 cases had mild liver injury before the treatment of TCM decoction. By comparing the liver function indexes before and after 1 week of TCM treatment, we found that the liver function of 2 cases recovered to normal after treatment while the other 14 cases still had mild liver injury, and there was no significant difference in liver function indexes before and after treatment (P>0.05). The liver function indexes of the other 84 cases were within the normal range before and after 1 week of TCM decoction treatment. ⑤In this study, 81 cases (81.0%) recovered and were discharged from hospital and 19 cases (19.0%) were still under treatment with a mortality rate of 0%. The average hospitalization duration of discharged patients was (16.0±5. 8) days. Of 5 severe cases of COVID-19,3 cases recovered from the disease and were discharged from hospital. The remaining 2 cases turned to mild type and did not develop to critical type. The conversion rate of critical case was 0%. ⑥Of 81 cases discharged from hospital, only 5 cases (6.2%) showed complete absorption of pneumonia infection, and the remaining 76 cases (93.8%) showed abnormal lesions of different degrees on chest CT. Conclusion:The integrated treatment of traditional Chinese and western medicine, especially the application of TCM decoction based on syndrome differentiation, can obviously reduce the main clinical symptoms of patients with COVID-19 such as fever, cough, poor appetite and diarrhea, etc., improve inflammatory reactions and immune imbalance of the body, reduce mortality and critical case conversion rate, and will not cause drug-induced liver injury.关键词：COVID-19;SARS-CoV-2;Chinese herbal medicine;integrated traditional Chinese and western medicine treatment;drug-induced liver injury;asymptomatic infection;outcome and prognosis;retrospective analysis
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发布时间：2022-08-31 - 摘要：This paper introduces Professor Xie Lin's clinical experience in the treatment of cervical spondylosis of vertebral artery type(CSA) from Xuanfu(sweat pore) theory. Based on Liu Wansu’s Xuanfu theory and combined with the etiology and pathogenesis of CSA, the Xuanfu theory of CSA was creatively put forward. It is considered that the basic pathogenesis is deficiency of kidney essence, loss of nourishment of the liver and spleen, atrophy of Xuanfu, stagnation of the liver and deficiency of the spleen, obstruction of Xuanfu due to stagnation of phlegm. The treatment method is resolving phlegm to dredge orifices, activating blood to promote qi flow, and helped with the methods of reinforcing the kidney to replenish essence, soothing the liver and invigorating the spleen. By using the methods, the goal is achieved of treating both symptoms and root causes. And the self-made empirical prescription－Geduna Dingxuan decoction is created, mainly composed of wind Chinese herbal medicines .关键词：cervical spondylosis of vertebral artery type;Geduan Dingxuan decoction;TCM therapy;Xuanfu;clinical experience;Xie Lin
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发布时间：2022-08-31 - 摘要：This paper introduces Yang Wei’s clinical experience in the treatment of anal fistula of Crohn’s disease with combination of Chinese and western medicine. It is considered that the pathogenesis is downward flow of damp-heat as the branch cause, and qi deficiency of the spleen and stomach as well as qi and blood deficiency of the liver and kidney as the root cause. In the treatment, Professor Yang advocates the combination of Chinese and western medicine, disease differentiation and syndrome differentiation, and the combination of medication and surgical treatment. She selects the timing of operation, pay attention to the protection of anal function, striving for fistula healing through staged operation and thorough drainage. And one proven case is presented.关键词：Crohn’s disease;anal fistula;therapy of integrated traditional Chinese and western Medicine;clinical experience;Yang Wei
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发布时间：2022-08-31 - 摘要：This article introduces a proven case of acupuncture combined with medicine in the treatment of tinnitus cranii in order to provide a reference for the treatment of tinnitus cranii in clinic.关键词：tinnitus cranii;acupuncture combined with medicine;proven case
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发布时间：2022-08-31 - 摘要：The incidence of tumors has been increasing due to the continuous extension of human life span and the rapid development of early diagnosis. Simultaneously, the improvement of the comprehensive curative effects of tumors has kept the tumor survival population expanding. Under the principle of adhering to traditional Chinese medicine (TCM) discipline development rules, we should inherit the essence of Chinese civilization, TCM classics and the practical experience of TCM doctors of past dynasties, and actively integrate modern scientific concepts and technologies such as oncology, immunology, system biology and artificial intelligence, etc. These views help to enrich the prevention and control theory and technical system of “strengthening vital qi to treat cancer”, and are further conducive to promoting the innovative development of oncology and the improvement of comprehensive curative effects.关键词：tumor;cancer;traditional Chinese medicine treatment;strengthening vital qi to treat cancer;tumor metastasis
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发布时间：2022-08-31 - 摘要：Objective:To explore the traditional Chinese medicine (TCM)syndrome types of ankylosing spondylitis(AS), and providethis article provided reference for the objective diagnosis of AS syndromes. MethodsInformations of 278 AS patients based on TCM four diagnostic methods were collected. Common factors were extracted and symptom loads were measured by factor analysis. Syndrome groups were summarized by systematic cluster analysis in order to determine the TCM syndrome types of AS. Results:24 common factors were extracted from clinical diagnostic informations of AS patients by factor analysis, and the factor scores were used as variables to cluster them.The basic TCM syndromes of AS could be divided into four categories, including nephrasthenia cold syndrome, yin-deficiency of kidney combined with yang-deficiency and dampness-heat syndrome, moist heat arthralgia spasm combined with spleen-deficiency syndrome, and stagnation of blood stasis syndrome. Conclusion:It was feasible that factor analysis combined with cluster analysis to induce AS syndromes, according to fundamental theories of TCM.关键词：ankylosing spondylitis;TCM syndromes;factor analysis;clustering analysis
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发布时间：2022-08-31 - 摘要：Objective:To investigate the expression difference of microRNAs (miRNAs) in urine of chronic hepatitis B (CHB) patients with liver-gallbladder dampness-heat (LGDH) syndrome and liver depression and spleen deficiency (LDSD) syndrome, to explore the characteristic miRNAs of different syndromes and to provide biological markers and objective basis for CHB syndrome differentiation and classification. MethodsUrine samples were collected from 53 subjects with LGDH syndrome, 53 subjects with LDSD syndrome and 24 healthy subjects. The expression levels of urinary miRNA were detected by miRNA microarray and miRNAs with differential expressions were analyzed; The quantitative reverse transcription-polymerase chain reaction (RT-qPCR) was used to detect the relative expressions of some differentially expressed miRNAs in urine of CHB patients with LGDH syndrome and LDSD syndrome. Prediction, GO and pathway analysis of miRNAs potential target genes were carried out. Receiver-operator characteristic (ROC) curves were generated to determine the specificity and sensitivity of each individual miRNA. The target genes of miRNAs were predicted and Gene Ontology (GO) and pathways were analyzed using bioinformatics methods. Results:①The microarray of miRNAs expression profiles showed that 21 miRNAs were up-regulated and 1 miRNA was down-regulated in CHB patients with LGDH compared with LDSD (fold change>1.5, P<0.05). ②The results from RT-qPCR revealed that miR-483-3p and miR-4700-3p were in significantly higher expression (P<0.05) and miR-4745-5p was in significantly lower expression (P<0.05) in the urine of CHB patients with LGDH syndrome compared with those with LDSD syndrome; miR-483-3p was in lower expression (P<0.05) and miR-4745-5p was in higher expression (P<0.05) in the urine of CHB subjects with LGDH syndrome LDSD syndrome and miR-4700-3p was in lower expression (P<0.05) in the urine of CHB subjects with LDSD syndrome compared with healthy subjects. ③ROC curve analysis exhibited the AUC (0.8106) and showed that these three miRNAs of miR-483-3p, miR-4700-3p and miR-4745-5p were sensitive and specific enough to distinguish LGDH and LDSD subjects. ④Bioinformatics methods showed that the difference of syndromes expressed potential target genes regulated by miRNAs. GO was mostly related to biological regulation, metabolic process, development of multicellular organisms, stress response and cell proliferation; Pathway was mostly related to post-translational protein modification, TGF-β signaling pathway, TGF-β family member signaling, TGF-β receptor complex signaling and other pathways. Conclusion:The miRNAs in the urine of CHB patients with LGDH syndrome and LDSD syndrome are differentially expressed. miR-483-3p, miR-4700-3p and miR-4745-5p may be potential markers for syndrome differentiation in CHB and these miRNAs may affect the development of syndromes by regulating their related target genes.关键词：chronic hepatitis B;liver-gallbladder dampness-heat syndrome;liver depression and spleen deficiency syndrome;urine;microRNAs;TCM syndrome differentiation
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发布时间：2022-08-31 - 摘要：Objective:To summarize the prescriptions and medication rules of Qianjin Fang (Essentially Treasured prescriptions) for treating low back pain based on the software of traditional Chinese medicine inheritance assistance system. MethodsThe prescriptions for the treatment of low back pain in Qianjin Fang were sorted out and the usage frequency of various Chinese herb medicines in the prescriptions and the association rules among Chinese herb medicines were analyzed by unsupervised data mining methods such as apriori algorithm of association rules and entropy clustering of complex system. Results:A total of 38 prescriptions were selected and the Chinese herb medicines used frequently in the top 5 were Rougui (Cortex Cinnamomi), Gancao (Radix Glycyrrhizae), Duzhong (Cortex Eucommiae), Fuling (Poria), Danggui (Radix Angelicae Sinensis); 14 core combinations of Chinese herb medicines and 7 new candidate drug groups were extracted. Conclusion:The prescriptions for low back pain in Qianjin Fang mostly have the functions of tonifying and purging, simultaneously regulating qi and blood, and focus on warming yang to dissipate cold, promoting qi flow to activate blood, tonifying the liver and kidney, invigorating qi to promote urination and dispelling wind to relieve exterior syndrome.关键词：Qianjin Fang;Sun Simiao;Low back pain;medication rules;data mining;association rules;clustering algorithm
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发布时间：2022-08-31 - 摘要：This paper introduces the clinical experience of Gao Jianzhong in using small-dose Xiaoqinglong decoction from the aspects of pathogenesis, prescription dosage, indications, modifying the prescription and so on. And one proven case is presented.关键词：Xiaoqinglong decoction;small-dose;clinical experience;Gao jianzhong
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发布时间：2022-08-31 - 摘要：This paper analyzes the thinking mode of the medication in Treatise on Cold Damage and Synopsis of the Golden Chamber. It is considered that on the basis of the theory of correspondence between prescription and syndrome, it is Zhang Zhongjing’s basic idea to adapt to the changes of diseases and syndromes through the combination or disassembly of classical prescriptions, the addition and subtraction of drug composition, and the changes of dosage and processing and decocting methods.关键词：Treatise on Cold Damage;Synopsis of the Golden Chamber;correspondence between prescription and syndrome;selection of treatment methods according to syndromes
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发布时间：2022-08-31 - 摘要：Objective:To explore the application value of Chinese medicine in enhanced recovery after surgery (CMERAS) for rectal cancer surgery. MethodsNinety patients with rectal cancer were randomly divided into treatment group and control group with 45 cases in each group. The control group adopted enhanced recovery after surgery (ERAS) for perioperative management, while the treatment group adopted CMERAS for perioperative management. The changes of TCM syndromes of the two groups were observed by using “TCM online" visual diagnostic instrument during the treatment process, and the clean degree of intestines during the operation, the first time of anal exhaust after the operation, the hospitalization time after the operation, the direct medical expenses and the occurrence of complications were compared between the two groups. Results: ①=1\*GB3Before treatment and on the first day after operation, there was no significant difference in the number of spleen deficiency syndrome cases, yin deficiency syndrome cases, qi deficiency syndrome cases, phlegm dampness syndrome cases and non-syndrome cases identified by visual diagnostic instrument between two groups (P>0.05). On the day of discharge, the number of patients with spleen deficiency syndrome, yin deficiency syndrome, qi deficiency syndrome and phlegm-dampness syndrome in the treatment group was less than that in the control group (P<0.05), and the number of non-syndrome cases was more than that in the control group (P<0.05). ②Intraoperative clean degree of intestines in the treatment group was better than that in the control group (P<0.05), the first time of anal exhaust after operation was earlier in the treatment group than that in the control group (P<0.05), the postoperative hospitalization time was shorter in the treatment group than that in the control group (P<0.05), and the direct medical expenses in the treatment group were less than that in the control group (P<0.05).③The incidence of complications in the treatment group and the control group were 4.4% and 2.2% respectively, with no significant difference (P>0.05). Conclusion:Compared with ERAS, CMERAS is beneficial to improve the pathological states of spleen deficiency, qi deficiency, phlegm-dampness and yin deficiency of rectal cancer patients after operation, accelerate postoperative rehabilitation, shorten hospitalization time and reduce medical expenses.关键词：rectal cancer;perioperative period;ERAS;CMERAS;postoperative rehabilitation;TCM syndrome
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of acupuncture treatment of TCM flaccidity syndrome of spleen and stomach deficiency with the method of “directing channel and guiding qi”. MethodsSixty patients with flaccidity syndrome (polyneuropathy) of spleen and stomach deficiency were randomly divided into treatment group and control group according to the random number table method, with 30 cases in each group. The control group maintained the patients' basic medication and gave mecobalamin tablets and Vitamin B1 for oral administration three times a day; The treatment group was given acupuncture to guide channel-qi besides the routine treatment, and acupoints on the Yangming and Shaoyang channels were selected on limbs affected. The course of treatment in both groups was 4 weeks, and the changes of sensory disturbance score, nerve conduction velocity and TCM syndrome score were observed. Results:①There were significant differences in sensory disturbance score, nerve conduction velocity and TCM syndrome score of both groups before and after treatment (P<0.05). ②After treatment, there were significant differences in sensory disturbance score, nerve conduction velocity and TCM syndrome score between the two groups (P<0.05), and the scores of the treatment group were significantly better than those of the control group. Conclusion:The acupuncture treatment of TCM flaccidity syndrome of spleen and stomach deficiency with the method of “directing channel and guiding qi” has achieved satisfactory clinical effects, which can significantly improve the sensory disturbance score, nerve conduction velocity and TCM syndrome score, and further help to improve patients’ quality of life.
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发布时间：2022-08-31 - 摘要：Objective:To analyze the distribution of TCM syndromes of obstructive sleep apnea hypopnea syndrome (OSAHS) in children and to explore the application value of polysomnography (PSG) in the diagnosis of OSAHS and differentiation of TCM syndromes. MethodsThe information of TCM syndromes and PSG data of 90 OSAHS children (case group) and 30 non-OSAHS children (control group) were collected to analyze the distribution of TCM syndromes, sex, age and disease severity of OSAHS children, and to compare the differences of PSG indexes between case group and control group as well as among cases with different TCM syndromes. Results:①Among 90 OSAHS cases, the top 10 symptoms or signs ranked by occurrence frequency were snoring, adenoid enlargement, mouth breathing, apnea or awakening due to suffocation, tonsil enlargement, daytime drowsiness, susceptibility to common cold, tiredness, hyperhidrosis and poor appetite. ②The most common TCM syndromes of OSAHS children were phlegm-dampness interaction syndrome (42.2%), followed by lung-spleen qi deficiency syndrome (31.1%) and lung meridian stagnation-heat syndrome (26.7%); Male OSAHS cases were more than female ones (P＜0.05). The number of OSAHS children aged 3-6 was more than that of OSAHS children aged 7-12 (P＜0.05). ③Compared with the control group, the obstructive apnea index (OAI) and apnea hypopnea index (AHI) increased in OSAHS children with lung meridian stagnation-heat syndrome, phlegm-dampness interaction syndrome and lung-spleen qi deficiency syndrome (P＜0.05), while average oxygen saturation (SM) and minimum oxygen saturation (SN) decreased (P＜0.05). Compared with the cases with lung meridian-heatstagnation syndrome and spleen-lung qi deficiency syndrome, OAI and AHI increased in OSAHS children with phlegm-dampness interaction syndrome (P＜0.05) while SM and SN decreased (P＜0.05). Conclusion:The most common TCM syndrome of OSAHS children is phlegm-dampness interaction syndrome. PSG is beneficial to the objectification and standardization of TCM syndrome differentiation in children with OSAHS.关键词：obstructive sleep apnea hypopnea syndrome;children;TCM syndrome;polysomnography
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发布时间：2022-08-31 - 摘要：Objective:To study the skincare effect of collagen peptide from Sika antlerbase and to optimize the process of extracting the collagen peptide by enzymatic hydrolysis. MethodsThe collagen extracted by hot water method as the raw material was digested with trypsin. The degree of collagen hydrolysis was used as an index. The single-factor test and Box-Behnken design test were used to optimize the preparation process of collagen peptide from the antlerbase. The antioxidation and reducing ability of the collagen peptide were investigated in the concentration range of 10,20,30,40 and 50 g/mL. Forty Kunming mice were randomly divided into blank group,control group,low,middle and high dose collagen peptide solution groups (0.1 g/L,0.2 g/L,0.4 g/L).The skin of mice was smeared with the peptide twice a day. At the end of 6 weeks,the activity of SOD,the content of hydroxyproline (Hyp) and the level of MDA were measured. Results:The antlerbase collagen comtent is 22.46%. The optimal preparation process of the antlerbase collagen peptide was enzymatic hydrolysis time 2.2 h,pH 8.0,temperature 50 ℃,enzyme amount 1 200 μ/g. Under this condition,the degree of protein hydrolysis is 15.97%.The collagen peptide of the antlerbase had the strongest antioxidation and reducing ability when the concentration reached 50 mg/mL. Compared with the blank group,the low,middle and high dose groups of collagen peptide had significant skincare effect as SOD and Hyp significantly increased (P<0.01) and MDA significantly decreased (P<0.01). Conclusion:The optimized enzymatic extraction conditions are stable and feasible,and the antlerbase collagen peptide has a good skincare effect.关键词：antlerbase;collagen peptide;hydroxyproline;SOD;MDA;mice
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发布时间：2022-08-31 - 摘要：Objective: To observe the effects of Gantang Yifang (GTYF) and its disassembled prescriptions—Fuzheng Huayu(FZHY)recipe and Shipi (spleen-firming) recipe on liver fibrosis and abnormal glucose metabolism in cirrhotic rats induced by CCl4. Methods A total of 98 SPF Wistar rats were randomly divided into 6 groups including normal group,model group,GTYF group,Shipi group,FZHY group and metformin group. All rats except the rats in the normal group were modelled by subcutaneous injection of 40%CCl4 olive oil solution combined with high-fat diet. From the fifth week of modeling, the rats in medication groups were given intragastric administration according to body mass once a day for 8 weeks. At 12 weeks, the glucose tolerance of rats was measured by OGTT test; the levels of blood glucose AUC and serum insulin were compared; and the insulin resistance index (HOMA-IR) was calculated. The body mass, liver mass and spleen mass of the rats were recorded, and the liver-to-body ratio and spleen-to-body ratio were calculated. Serum and tissue samples were collected to detect the levels of serum ALT, AST, Alb and TBiL. Liver steatosis was observed by HE staining, collagen deposition by Sirius red staining, and the content of hydroxyproline (Hyp) in liver tissue determined by colorimetry. Results:Compared with the model group, GTYF and FZHY recipe could significantly improve the abnormal glucose tolerance, reduce the blood glucose AUC (P<0.05) and HOMA-IR (P<0.05), and the fasting insulin concentration in the Shipi recipe group was significantly lower than that in the model group (P<0.05). The results of serum biochemical test showed that GTYF and Shipi recipe could reduce the level of ALT (P<0.05) and Shipi recipe could also reduce the level of AST (P<0.05). The results of HE staining showed that GTYF could significantly reduce the steatosis of hepatocytes, and Sirius red staining showed that GTYF and FZHY recipe could significantly reduce the collagen area of the liver and the content of Hyp in liver tissue (P<0.05,P<0.01). Conclusion:GTYF and its disassembled prescriptions—FZHY recipe and Shipi recipe can relieve hepatogenic diabetes induced by CCl4 combined with high-fat diet by reducing hepatic steatosis and fibrosis, regulating and improving abnormal glucose tolerance and insulin resistance.关键词：Gantang YiFang;abnormal glucose tolerance;insulin resistance;liver fibrosis;abnormal glucose metabolism;hepatogenic diabetes;rats
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发布时间：2022-08-31 - 摘要：Objective:To observe the effect of salidroside on erythropoietin (EPO) level and bone marrow pathology in rats with high altitude polycythemia (HAPC) after intervention, and to explore the mechanism of salidroside in preventing and treating HAPC.MethodsForty SPF Wistar rats were randomly divided into normal control group, model group and high, middle and low dose groups with 8 rats in each group. Except the normal control group, the other 4 groups duplicated HAPC rat models according to the methods from the literature. Salidroside was administered to the high, middle and low dose groups by gavage at doses of 200 mg/kg, 100 mg/kg and 50 mg/kg respectively, while the equal volume of 0.9% NaCl solution was administered to the model group and the normal control group by gavage respectively. All groups were intervened for 40 days.The levels of EPO in serum and kidney were determined by ELISA and Western blot respectively. Pathological smears of bone marrow were made. After HE staining, the morphology, classification and cytological counting of bone marrow cells were observed under microscope. Results:①Compared with the model group, EPO levels in kidney and serum of rats in the high, middle and low dose groups were significantly reduced, and the difference was statistically significant (P<0.01). In particular, the down-regulation of EPO level in serum was significantly dose-dependent. ②The bone marrow erythron of rats in the low dose group proliferated actively, mainly the orthochromatic erythroblasts; Compared with the bone marrow erythron of rats in the model group, no obvious morphological change was found in the low dose group, and there was no significant difference in each index (P>0.05).③Although there was erythroid hyperplasia in the bone marrow of rats in the middle dose group, the hyperplasia of orthochromatic erythroblasts and mature erythrocytes was not significant, the morphology of mature erythrocytes was normal, and the ratio of granulocyte to nucleated erythrocyte was significantly different from that in the model group (P<0.05).④The proportion of bone marrow granulocyte series and erythron in the high dose group tended to be normal.Compared with the model group, the proliferation of orthochromatic erythroblasts was not active, the morphology of mature erythrocyte was normal, and the proportion of nucleated erythrocyte, the proportion of granulocyte and the ratio of granulocyte to nucleated erythrocyte in the test were significantly different (P<0.01). Conclusion:Salidroside can regulate the overexpression of EPO in HAPC rats and improve the excessive compensatory proliferation of bone marrow erythron.关键词：high altitude polycythemia;rats;salidroside;erythropoietin;bone marrow pathology
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发布时间：2022-08-31 - 摘要：Skeletal muscle dysfunction is a prominent manifestation of extrapulmonary effect of chronic obstructive pulmonary disease (COPD). Due to the low physical activity level, systemic inflammation, hypoxia and other reasons, changes in structural and metabolic characteristics that occur in the early stage of the disease, including skeletal muscle mass reduction, cross-sectional muscle area decrease, reduction of total muscle fiber amount, muscle fiber-type shifting, capillary density decrease, mitochondrial dysfunction, etc., may cause weakening of skeletal muscle strength and endurance of patients, affect their exercise performance, increase hospitalization rate and mortality rate, and seriously affect their quality of life. As a unique form of exercise in China, traditional Chinese medical exercise (Gongfa) belongs to the low and moderate intensity aerobic exercise and is an effective exercise rehabilitation method as it can effectively slow down and improve the structural changes of skeletal muscle, improve its muscle strength and enhance its muscle endurance by reducing the level of inflammatory factors and improving blood oxygen level in COPD patients.关键词：chronic obstructive pulmonary disease;skeletal muscle;dysfunction;traditional Chinese medicine;traditional Chinese medical exercise;exercise rehabilitation;lung rehabilitation
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发布时间：2022-08-31
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