最新刊期
期 6 ， 2020
- 摘要：The clinical progress of traditional Chinese medicine (TCM) in history was closely related to the high incidence of epidemic diseases. At the end of Han dynasty and in Wei and Jin dynasties, On Cold Damage was written. During Jin and Yuan dynasties, there were fire-heat theory and On Spleen and Stomach due to epidemic diseases. During Ming and Qing dynasties, the school of warm diseases emerged after the completion of the book On Plague Diseases. In the process of changing and development of epidemic diseases, TCM formed basic methods of syndrome differentiation and treatment and the system of clinical diagnosis and treatment. TCM clinical progress has never stopped.关键词：epidemic disease;TCM clinical development history;On Cold Damage;fire-heat theory;On Spleen and Stomach;On Plague Diseases;On Warm-Heat
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发布时间：2022-08-31 - 摘要：The methods of preventing and treating coronavirus diseases 2019 (COVID-19) with traditional Chinese medicine was discussed based on the Treatise on Spleen and Stomach, which was written due to the Bianjing pestilence. Li Dongyuan attached great importance to strengthening body resistance for the treatment of internal injuries, whose therapeutic thinking is of great significance to the prevention and treatment of epidemic diseases. Based on the analysis of the pathogenic characteristics, clinical characteristics and pathological immune responses of COVID-19, it is believed that the occurrence and progress of the disease are closely related to the deficiency of the spleen and stomach and the yin-fire overwhelming the lungs. Nourishing the spleen and stomach and purging yin-fire can be used to prevent and treat SARS-CoV-2 infection and severe COVID-19.关键词：COVID-19;SARS-CoV-2;Treatise on Spleen and Stomach;nourishing the spleen and stomach;purging yin-fire;Bupiwei Xieyinhuo Shengyang decoction (decoction for nourishing the spleen and stomach, purging yin-fire and promoting yang)
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发布时间：2022-08-31 - 摘要：The application strategy of six meridian syndrome differentiation method was analyzed for the treatment of coronavirus disease 2019 (COVID 19). Through the analysis of clinical symptoms and the summary of prescriptions and treatment protocols, it was believed that the syndrome differentiation of the “pandemic” can be attributed to the six meridians, and the prescriptions used for prevention and treatment also conformed to the classical herbal prescriptions’ principle of correspondence between prescription and syndrome. Therefore, six meridian syndrome differentiation method can be used for the diagnosis and treatment of COVID 19. One cured case of COVID 19 was provided for exemplification.
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发布时间：2022-08-31 - 摘要：The application of traditional Chinese medicine (TCM) purgative method to the treatment of coronavirus disease 2019 (COVID-19) was explored from the aspects of eliminating pathogens and reducing fever, truncating and reversing, removing residual pathogens, dredging bowels and resolving dampness, etc. It is believed that the purgative method has unique functions in reducing fever, improving intestinal dysfunction symptoms, preventing the critical development of disease, promoting the negative conversion of fecal virus nucleic acid test and facilitating rehabilitation. The application timing should be selected wisely and appropriately and relevant precautions should be noted. One cured case was presented as the exemplification.关键词：COVID-19;SARS-CoV-2;warm disease;purgative method;traditional Chinese medicine treatment
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发布时间：2022-08-31 - 摘要：TCM treatment of coronavirus disease 2019 (COVID-19) based on “truncation and reversal” strategy was discussed. It is believed that implementing treatment measures in advance before the occurrence of corresponding syndromes is beneficial to quickly control the disease condition and truncate the disease progress. Commonly used therapeutic methods include invigorating spleen and assisting transportation, activating qi and resolving dampness, dredging fu organs and attacking the lower, cooling blood and removing blood stasis, eliminating phlegm and softening hard mass, and removing pestilential qi, etc.关键词：COVID-19;SARS-CoV-2;damp-heat pestilence;truncate and reverse;implementing treatment measures before the occurrence of corresponding syndromes;traditional Chinese medicine treatment
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发布时间：2022-08-31 - 摘要：COVID-19 belongs to the category of damp pestilence in Chinese medicine. The key pathogenesis is plague poison blocking the lung caused by exogenous turbid dampness and plague poison lurking the pathogenhidde, motivating shaoyang fire, wood-fire impairing the metal and dampness poison fire uprush. In treatment of COVID-19, we use harmonic method throughout the whole process, strengthening and consolidating basis by protecting middle qi, pectoral qi, and yin fluid. The basic therapeutic principle are dredging qi activity and clearing heat, with combination of frigotherapy and pyretotherapy. Multiple methods are mixed by freeing lung, clearing heat and detoxifying, boosting qi and nourishing yin, activating blood and dissolving stasis, resolving dampness and dredging collaterals, aiming at cutting off the progress of the disease and saving lives.关键词：COVID-19;SARS-CoV-2;damp pestilence;harmonic method;protecting middle qi;protecting pectoral qi;protecting yin fluid
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发布时间：2022-08-31 - 摘要：At present, there is still a lack of clinical evidence with clear efficacy in the prevention and treatment of coronavirus disease 2019 (COVID-19). The considerations and suggestions for further traditional Chinese medicine clinical research of COVID-19 were discussed from the aspects of medical ethics, research fields, research protocols, design of randomized controlled trials, real world research and clinical trial cooperation, etc. for the purpose of providing reliable evidence for the evidence-based medicine (EBM).关键词：COVID-19;SARS-CoV-2;clinical trial;EBM
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发布时间：2022-08-31 - 摘要：Objective:sTo systematically evaluate the clinical efficacy of integrated traditional Chinese and western medicine in the treatment of coronavirus disease 2019 (hereinafter referred to as “COVID-19”). MethodsData were collected by searching Wanfang DATA, CNKI, MEDLINE and Embase databases from the creation date of these databases to March 12,2020. The risk of bias was assessed according to the Cochrane Manual “Risk of Bias” assessment tool. Newcastle-Ottawa Scale (NOS) was used to evaluate the methodological quality of non-random trials, and the quality of evidence was measured by GRADE. Review Manager 5.3 software was used for meta-analysis on the outcome indexes of different intervention measures in the treatment of COVID-19, and comparison of the clinical effects between the treatment group (integrated treatment of traditional Chinese and western medicine) and the control group (conventional western medicine treatment). Results:①A total of 8 clinical studies were included, containing 804 cases. ②One of the studies was a randomized controlled trial, four were retrospective cohort studies, one was before-after study in the same patient, and two were case series studies. Five studies were included in meta-analysis, and three studies without control group were only evaluated by NOS. ③The conversion rate of severe cases was compared and analyzed in two studies. The results showed that the conversion rate of severe cases in the treatment group was lower than that in the control group [RR=0.18,95% CI(0.04,0.79), P=0.02]. ④The clinical cure rate was compared and analyzed in three studies. The results showed that the clinical cure rate of the treatment group was higher than that of the control group [OR=7. 82,95% CI(2.04,30.06), P=0.003]. ⑤The mortality rate was analyzed in two studies. The results showed that there was no significant difference between the two groups [RR=0.18, 95% CI(0.01,4.23), P=0.29], and no heterogeneity conclusion could be drawn. ⑥The fever duration was compared and analyzed in four studies, and there were obvious heterogeneity among the studies (P=0.003, I2=84%). The results suggested that the antipyretic effect of the treatment group was better than that of the control group [MD=-0.81,95% CI(-1.53, -0.09), P=0.03], but the further analysis on the sources of heterogeneity was required. ⑦The symptom scores were compared and analyzed in two studies. The results showed that the clinical symptoms of the patients were better improved in the treatment group than in the control group [SMD=-0.84,95% CI(-1.25, -0.43), P<0.000 1]. ⑧The remission rate of cough was compared and analyzed in three studies. The results showed that the cough symptom was better relieved in the treatment group than in the control group [OR=5. 14,95% CI(1.49,17.75), P=0.010]. ⑨The remission rate of diarrhea was compared and analyzed in three studies. The results showed that there was no significant difference between the two groups [OR=1. 19,95% CI(0.17,8.64), P=0.86]. ⑩The absorption rate of pneumonia was compared and analyzed in three studies. The results showed that there was no significant difference between the two groups [RR=1.02,95% CI(0.85,1.22), P=0.87]. Conclusion:The integrated treatment of traditional Chinese and western medicine can reduce the conversion rate of severe cases, improve the clinical cure rate, and has certain advantages in relieving cough and fever among patients with COVID-19. In order to improve the evidence integrity of this study, more high-quality clinical evidence-based studies are needed for data update.关键词：COVID-19;SARS-CoV-2;integrated Chinese and Western medicine therapy;Meta-analysis;systematic review;clinical research
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发布时间：2022-08-31 - 摘要：Based on the study of the understanding of epidemic disease in traditional Chinese medicine(TCM), the influence of Yunqi theory on epidemic situation and the characteristic of prescriptions and medicines for the prevention and treatment of epidemic disease in TCM, this paper discusses the practical significance of ancient Chinese anti epidemic theory and its clinical guiding value for COVID-19 prevention and treatment. It is believed that combined with the theory of Yunqi, the cause of COVID-19 is that the water and fire are mutually controlled at the end of 2019. It is estimated that the large-scale spread of COVID-19 will be controlled in March 2020 and eliminated in June to August. The high-frequency TCM for the prevention and treatment of COVID-19 is mainly aromatic resuscitation medicine, heat clearing and detoxification medicine, wind dispelling and deficiency tonifying medicine,but the way of formulation is different due to the different stages of the disease.关键词：coronavirus disease 2019;SARS-CoV-2;therapy of traditional Chinese medicine;infectious diseases;Yunqi;data mining
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发布时间：2022-08-31 - 摘要：This paper analyzes the clinical experience of Professor Wang Xiaomin in treating advanced tumors from the perspective of yang deficiency and toxin accumulation. He believes that the pathogenesis of advanced tumors is the mixture of deficiency and excess, with yang deficiency in the whole and toxin heat in the local and treats the disease based on the principle of strengthening healthy qi and reinforcing deficiency, warming yang and eliminating stagnation, clearing away heat and removing toxin, and expelling stasis and dredging collaterals. Professor Wang Xiaomin is proficient in using Huangqi (Radix Astragali) and Dangshen (Radix Codonopsis) to strengthen healthy qi and reinforce deficiency, Fuzi (Radix Aconiti Lateralis Preparata) and Ganjiang (Rhizoma Zingiberis) to warming yang and eliminating stagnation, Banzhilian (Herba Portulacae Grandiflorae) and Baihua Sheshecao (Herba Hedyotis Diffusae) to clearing away heat and removing toxin, and Tubichong (Eupolyphaga Seu Steleophaga) and Chuanshanjia (Squama Manitis) to expelling stasis and dredging collaterals.关键词：yang deficiency and toxin accumulation;tumors;specialist experience;Wang Xiaomin
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发布时间：2022-08-31 - 摘要：Professor Que Huafa’s experience in the treatment of thyroid cancer was introduced. It was suggested that the pathogenesis of the disease was due to the deficiency of vital energy and the stagnation of toxin,he emphasized that the treatments of this disease were based on the strengthening of vital energy and removing pathogenic factors. It was necessary to review for differentiation and regulating the spleen and kidney. Not only combination of disease and syndrome but also differentiation of meridians should be concerned. He was expert in using the drugs to guide the meridians. He paid more attentions to the constitution differentiation, and advocated strengthening yang qi.One proven case was presented.关键词：thyroid cancer;treatment of traditional Chinese medicine;expert experience;Que Huafa
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发布时间：2022-08-31 - 摘要：Professor Wang Xixing’s clinical experience in differentiating and treating colorectal carcinoma (CRC) liver metastasis was introduced. It is believed that the fundamental pathogenesis of CRC liver metastasis is the dual deficiency of the liver and spleen and accumulation of phlegm and blood stasis. Clinically, both the root and branches of the disease are considered and the reinforcing and reducing methods are applied with regulating and harmonizing the liver and spleen as the priority and decreasing toxicity and eliminating mass as the complement. Buzhong Tiaogan (middle-tonifying and liver-regulating) decoction, a special prescription formulated to fight against cancer with the harmonizing method, has achieved great clinical effects. One case was attached for exemplification.关键词：colorectal carcinoma;liver metastasis;regulating and harmonizing the liver and spleen;decreasing toxicity and eliminating mass;harmonizing method;famous doctor experience;Wang Xixing
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical effect of the Miaoling Xianzi decoction for the syndrome of accumulated dampness-heat of acute gouty arthritis. MethodsNinety patients with the syndrome of accumulated dampness-heat of acute gouty arthritis were randomly divided into three groups.The tradition-medicine group was treated with Miaoling Xianzi decocti]on.The modern-medicine group was treated with colchicine.The tradition-modern medicine group was treated with Miaoling Xianzi decoction and colchicine.There were 30 cases in each group,which was followed up for 1 month,and recorded joint pain score,joint swelling score,joint function score,TCM symptom score,uric acid(UA) level,inflammatory cytokines,kidney function and adverse reactions before and after treatment in each group.Calculate the time required for the reduction of colchicine in the modern-medicine group and the tradition-modern medicine group. Results:①After treatment,the joint pain score,joint swelling score,and joint function score of the tradition-modern medicine group were lower than tradition-medicine group and modern-medicine group(P<0.05).There was no significant difference between the tradition-medicine group and modern-medicine group(P>0.05).②After treatment,the improvement of TCM symptoms of the tradition-medicine group and the tradition-modern medicine group is better than that of modern-medicine group(P<0.05).③The efficacy of uric acid(UA)of the tradition-medicine group and the tradition-modern medicine group was significantly better than the modern-medicine group(P<0.01).There was no statistical difference in the White blood cells(WBC), Erythrocyte sedimentation rate(ESR),C-reactive protein(CRP),Serum creatinine(SCR)and Blood urea nitrogen(BUN) in three groups(P>0.05).④The adverse reaction rate of the tradition-medicine group and the tradition-modern medicine group were significantly lower than the modern-medicine group(P<0.05).⑤The time required for colchicine reduction in the tradition-modern medicine group was less than the modern-medicine group(P<0.05). Conclusion:Miaoling Xianzi decoction is not inferior to anti-inflammation and to relieve pain than colchicine in the treatment for accumulated dampness-heat of acute gouty arthritis,with the effect of lower uric acid and lower probability of adverse reactions.关键词：acute gouty arthritis;accumulated dampness-heat;tradition-medicine treatment;Miaoling Xianzi decoction
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发布时间：2022-08-31 - 摘要：Based on 10 fever cases caused by different diseases,syndromes and causes,we can tell that fever can be caused by external contraction or internal injuries. Therefore,the syndrome differentiation and treatment of fever is complicated.The idea of traditional Chinese medicine for the treatment of fever requires the combination of disease differentiation and syndrome differentiation,the identification of deficiency and excess,and cause deduction based on syndrome analysis.关键词：fever;external contraction;internal injury;deducing the cause by analyzing the syndrome;treatment based on syndrome differentiation;traditional Chinese medicine theory
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发布时间：2022-08-31 - 摘要：Epigenetic modifications mainly include DNA, RNA and histone modifications. The modifications include various forms, including methylation, acetylation, lactylation, and glycosylation, etc. Epigenetic modifications play an important role in the occurrence and development of tumors, which could be applied to the early diagnosis and prognosis evaluation of tumors, and could also prompt a new direction for the targeted therapy strategy of tumors. Traditional Chinese Medicine could play an anti-tumor role by regulating epigenetic modifications. Epigenetic antitumor drugs mainly include DNMT inhibitors, HDAC inhibitors and IDH inhibitors, which have been applied in clinical trials. The research on RNA methylation is still in its infancy and there are no approved drugs, but its important function in tumors has been confirmed. Therefore, DNA, RNA and histone modification could be used as diagnostic or prognostic markers for tumors, and the development of anti-tumor drugs targeting DNA, RNA and histone modification enzymes will become a new direction for tumor treatment.关键词：epigenetic modification;DNA modification;RNA modification;histone modification;tumor diagnosis;regulation and control of traditional Chinese medicine;epigenetic antitumor drug
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发布时间：2022-08-31 - 摘要：Objective:To obtain the core traditional Chinese medicine (TCM) syndrome elements and western medicine physical examination indexes of sub-health population through complex network analysis, and to provide multi-dimensional clinical basis and reliable methods for distinguishing TCM excess syndromes in sub-health population. MethodAccording to the classification criteria of health status, we screened 233 cases of sub-health status diagnosed with TCM excess syndrome, including 105 cases of qi stagnation syndrome, 69 cases of blood stasis syndrome, 34 cases of phlegm-dampness syndrome and 25 cases of excess heat syndrome. The complex network analysis was used to analyze the core network relationship between TCM syndrome elements of each syndrome and western medicine physical examination indexes. Results:①The core syndrome elements of Qi stagnation syndrome were epigastric stuffiness and distension, distending pain of chest and hypochondrium and thick coating, etc. The core physical examination indexes of western medicine were abnormal diastolic blood pressure and monocyte level. The associated nodes of the two groups of items were epigastric stuffiness and distension, belching, thick coating, fatigue and abnormal diastolic blood pressure. ②The core syndrome elements of blood stasis syndrome were pale purple tongue, white coating and thin left pulse, etc. The core physical examination indexes of western medicine were abnormal systolic blood pressure, basophil level and platelet distribution width, etc. The associated nodes of the two groups of items were pale purple tongue, white coating, wiry left pulse, thin left pulse, abnormal systolic blood pressure, diastolic blood pressure, basophilic granulocyte level, platelet distribution width and monocyte level. ③The core syndrome elements of phlegm-dampness syndrome were greasy coating, dizziness and insomnia, etc. The core physical examination indexes of western medicine were abnormal body mass index (BMI), uric acid and total cholesterol, etc. The associated nodes of the two groups of items were yellow coating, thick coating, greasy coating, abnormal systolic blood pressure, monocyte level, platelet distribution width and body mass index. ④The core syndrome elements of excess heat syndrome were gingival swelling and pain, yellow coating and constipation, etc. The core physical examination indexes of western medicine were abnormal BMI, systolic blood pressure, monocyte level, etc. The associated nodes of the two groups of items were constipation, yellow coating, abnormal systolic blood pressure, diastolic blood pressure, basophil level and monocyte level. Conclusion:The complex network analysis can be used to find the core TCM syndrome elements and core western medicine physical examination indexes in sub-health population with different excess syndromes, and to provide more accurate and multi-dimensional basis for distinguishing TCM excess syndromes in sub-health population. Specific physical examination indexes corresponding to different TCM syndromes are helpful to find abnormal manifestations of sub-health population from the perspective of western medicine, which may further help early intervention to reduce the risk of suffering from certain diseases.关键词：sub-health population;complex network;TCM syndrome;excess syndrome;physical examination indexes
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发布时间：2022-08-31 - 摘要：Objective:To study the characteristics of tongue images, pulse conditions and medication rules of seasonal pathogen induced diseases in children based on the data mining method. MethodsA standardized database was established by including medical records of 634 cases of seasonal pathogen induced diseases in children extracted from the Collection of Pediatric Medical Records of Past Dynasties, and the correlation between tongue images, pulse diagnosis and Chinese herbal medicines (CHMs) and the medication rules were analyzed by using frequency statistics, logistic regression analysis, association rules and other methods. Results:①A total of 634 cases of medical records from Han dynasty to modern times were included, involving 634 prescriptions and 250 kinds of CHMs. ②There were 23 kinds of seasonal pathogen induced diseases in children, most of which were exogenous warm febrile diseases. There were 16 kinds of tongue images with frequency greater than or equal to 4. The tongue images mainly indicated a heat syndrome, including red tongue, crimson tongue and prickly tongue, etc., with greasy coating, yellow coating, white coating or thick coating, etc. There were 16 kinds of pulse conditions with frequency greater than or equal to 3, most of which were rapid pulse, slippery pulse, wiry pulse and fine pulse, etc. There were 22 kinds of CHMs with frequency greater than or equal to 82, which were Xingren (Armeniacae Semen Amarum), Gancao (Radix Glycyrrhizae), Lianqiao (Fructus Forsythiae), etc. ③There were 15 strong correlations between tongue images and CHMs. For instance, red tongue had a strong correlation with Digupi (Cortex Lycii), Zhuli (Succus Bambusae) and Gualou (Fructus Trichosanthis); greasy coating had a strong correlation with Paojiang (Rhizoma Zingiberis Preparata), Peilan (Herba Eupatorii) and Mahuang (Herba Ephedrae); and yellow coating had a strong correlation with Dangshen (Radix Codonopsis), Huanglian (Rhizoma Coptidis) and Zhishi (Fructus Aurantii Immaturus). ④There were 13 strong correlations between pulse conditions and CHMs. For instance, rapid pulse had a strong correlation with Douchi (Semen Sojae Preparatum), Gegen (Radix Puerariae) and Xuanshen (Radix Scrophulariae); slippery pulse had a strong correlation with Zhuli (Succus Bambusae), Rendongteng (Caulis Lonicerae) and Digupi (Cortex Lycii); and wiry pulse had a strong correlation with Xiakucao (Prunella Vulgaris), Juluo (Citri Reticulatae Exocarpium Album) and Rendongteng (Caulis Lonicerae), etc. ⑤The drug pairs with high confidence were Pipaye (Folium Eriobotryae) and Zhebeimu (fritillaria thunbergii), Ziwan (Radix Asteris) and Xingren (Armeniacae Semen Amarum), Bohe (Herba Menthae) and Lianqiao (Fructus Forsythiae), etc. The drug pairs with high support were Zhebeimu (fritillaria thunbergii) and Xingren (Armeniacae Semen Amarum), Jinyinhua (Flos Lonicerae) and Lianqiao (Fructus Forsythiae), Shanzhizi (Fructus Gardeniae) and Lianqiao (Fructus Forsythiae), etc. ⑥The correlations between tongue images and pulse conditions with high confidence were yellow coating and rapid pulse, white coating and rapid pulse, red tongue and rapid pulse, etc. The correlations between tongue images and pulse conditions with high support were red tongue and rapid pulse, greasy coating and rapid pulse, yellow coating and rapid pulse, etc. Conclusion:Seasonal pathogen induced diseases recorded in the Collection of Pediatric Medical Records of Past Dynasties mostly have damp-heat tongue images, pulse conditions are rapid pulse, slippery pulse and wiry pulse, and most CHMs used are Xingren (Armeniacae Semen Amarum), Gancao (Radix Glycyrrhizae) and Lianqiao (Fructus Forsythiae), etc. There are correlations among certain tongue images, pulse conditions and CHMs used and association rules in the compatibility of CHMs. Modern pediatric clinical practice of traditional Chinese medicine may learn from the experience of diagnosis and treatment in pediatric medical records of previous dynasties.关键词：epidemic diseases;children;tongue image;pulse condition;traditional Chinese medicine diagnosis;Chinese herbal medicine;rule of diagnosis and treatment;data mining;regression analysis
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发布时间：2022-08-31 - 摘要：Obstructive sleep apnea syndrome(OSA) is an independent risk factor for hypertension. Because of it being ignored as the cause of hypertension,the patients with OSA-related hypertension are often missed and delayed. The cognition of OSA in patients with hypertension was surveyed by using the Sleep Apnea Cognitive Questionnaire for Patients with Hypertension. The concealment,clinical characteristics and pathogenesis of OSA-related hypertension were analyzed so as to explore its thought way of lowering blood pressure. It was suggested that the patients should postpone the choice of western medicine to directly lower the blood pressure after trying to adjust their life style to lower blood pressure and obtaining poor effect,and try to relieve the symptoms of OSA with Chinese medicine,meanwhile,the continuous positive airway pressure(CPAP) was used to improve the ventilation condition,thereby to regulate the blood pressure and recover the normal circadian rhythm of blood pressure.关键词：obstructive sleep apnea syndrome;hypertension;concealment;cognitive survey;thought way of lowering blood pressure
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发布时间：2022-08-31 - 摘要：Objective:To observe the effect of electroacupuncture at Zusanli and Changqiang points on the physiological function of anal sphincter in rats with neurogenic fecal incontinence and to explore its mechanism. MethodsNinety healthy male SD rats were selected and randomly divided into the model group, electrical stimulation group and electroacupuncture group, with 30 rats in each group, and another 6 healthy male SD rats were set as blank group. Except the blank group, the fecal incontinence models were established in other three groups of rats by cutting off bilateral pudendal nerves. After successful modeling, the electroacupuncture group and the electrical stimulation group were continuously intervened for 14 days, while the model group and the blank group were not intervened. The stool condition of rats was observed, and related indexes of the physiological function of anal sphincter (anorectal manometry and myoelectric activity of external anal sphincter) were detected. HE staining was used to observe the pathological changes of anal sphincter tissue of rats in each group. Results:①=1\*GB3In the blank group, the padding in the box was dry and the feces were granular. In the model group, the padding was wet and the feces were thin and mushy. The feces of rats in the electrical stimulation group and the electroacupuncture group gradually formed from mush to soft spherical shape. The feces in the electroacupuncture group improved more significantly and their texture was harder. ②=2\*GB3Compared with the blank group, the anorectal manometry in the model group decreased (P<0.05), and the myoelectric activity of the external anal sphincter decreased (P<0.05). ③=3\*GB3Compared with the model group, there was no significant difference in anorectal manometry and myoelectric activity of external anal sphincter in the electrical stimulation group at each observation time point (P>0.05). In the electroacupuncture group, the anorectal manometry increased on day 3, day 7 and day 14 after treatment (P<0.05), and the myoelectric activity of the external anal sphincter increased on day 7 and day 14 after treatment (P<0.05). ④Compared with the electrical stimulation group, the anorectal manometry in the electroacupuncture group was higher than that in the electrical stimulation group on day 3 and day 14 (P<0.05), and the myoelectric activity of the external anal sphincter was stronger than that in the electrical stimulation group on day 7 and day 14 (P<0.05). ⑤The muscle fibers of the external anal sphincter of rats in the blank group were arranged densely and orderly. In the model group, the muscle fiber structure was disordered, the muscle fiber space was widened, and the external anal sphincter atrophy occurred to varying degrees. The structure and arrangement of muscle fibers in electrical stimulation group and electroacupuncture group were significantly improved, and the improvement in electroacupuncture group was more significant. Conclusion:Electroacupuncture at Zusanli and Changqiang points can effectively improve the fecal incontinence of neurogenic fecal incontinence rats, and its mechanism may be related to increasing anorectal manometry and enhancing myoelectrical activity of external anal sphincter.关键词：fecal incontinence;neurogenic;electroacupuncture;anorectal manometry;external anal sphincter;electric activity;rats
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发布时间：2022-08-31 - 摘要：Objective:To observe the regulation effect of AT1 receptor on the reproductive system of male mice,and preliminarily explore the mechanism of improving the reproductive function of ♂AT1+/－mice by Wuzi Yanzong pills, a traditional Chinese medicine compound. MethodsTwenty 8-week-old C57BL/6 normal male mice and 40 AT1KO heterozygous male mice(♂AT1 +/－) were randomly divided into group A(normal male mice group), group B(♂AT1 +/－mice group),and group C(♂AT1+/－mice + Wuzi Yanzong pills group). The mice in group A and group B were given intragastric administration with 0.9% NaCl solution,and the mice in group C with Wuzi Yanzong pills at 5.8 g/kg, twice daily with 0.5 mL each time. The expression of AT1 in testis, hypothalamus and pituitary was determined by RT-PCR method. Pregnancy rate was measured before sampling,and sperm morphology was observed in each group. Plasma sex hormone levels, including gonadotropin releasing hormone (GnRH), follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone (T),were measured by ELISA enzyme immunoassay. Results:Compared with the normal group, only a small amount of AT1 was detected in testis,pituitary and hypothalamus tissues in both groups of B and C(P<0.01), indicating that the model was successful. None of the female mice in cages with mice from group B and C were pregnant, but the sperm morphology in group B and C was significantly abnormal as compared with that in the normal group (P<0.01). After the intervention of Wuzi Yanzong pills, the normal rate of sperm was significantly increased, and there was significant difference between group C and group B (P<0.05). The level of GnRH in group B was significantly higher than that in group A (P<0.01). After the intervention of Wuzi Yanzong pills, the levels of GnRH and FSH in group C were significantly higher than those in group B (P<0.05).The levels of GnRH, FSH and LH in group C at the 4th week were higher than those in the second week of the same group (P<0.05). Conclusion:The knockout of AT1 gene can lead to infertility in male mice. Wuzi Yanzong pills may improve the reproductive function of male mice by increasing the level of sex hormones and the normal rate of spermatozoa in AT1+/- mice.关键词：renin-angiotensin system;infertility;mice;kidney governing reproduction;Wuzi Yanzong pills
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发布时间：2022-08-31
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