最新刊期
期 12 ， 2020
- 摘要：Cases of cold damage diseases recorded in TCM classic book-On Cold Damage introduced the general scope and identification methods of epidemic diseases. TCM doctors in Ming and Qing dynasties paid more attention to the induction and summary of therapeutic methods and formulas and pursued clinical practicality. With in-depth thinking from different angles such as the name and nature of the disease, the rules of treatment and clinical thinking mode, etc., it is believed that the ancient experience of syndrome differentiation and treatment of epidemic diseases can still guide our clinical practice today.关键词：COVID-19;epidemic disease;On Cold Damage;case of cold damage diseases
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发布时间：2022-08-31 - 摘要：Objective:To investigate the distribution and general pattern of TCM syndromes of knee osteoarthritis (KOA) among the middle-aged and elderly people in Shanghai community. MethodsThe middle-aged and elderly KOA patients were selected from 33 communities in 9 municipal districts of Shanghai from July 1,2016 to September 1,2018 by using the questionnaire of TCM syndromes of knee osteoarthritis. The general data, course of disease, local symptoms of knee joints, general symptoms, tongue and pulse conditions and X-ray findings of knee joints were collected and statistically analyzed by professional TCM orthopedists and traumatologists to determine TCM syndromes, and the correlation between TCM syndromes and age, sex, course of disease and X-ray grading was also analyzed. Results:①A total of 1,990 KOA patients were included in the study, including 484 males and 1,506 females. The age ranged from 45 to 85 years old, with an average age of 66.0±5.8 years old. The course of disease lasted 1 to 480 months, and the median course of disease was 24 months. There were 702 cases of cold-dampness obstruction syndrome, 542 cases of liver and kidney deficiency syndrome, 406 cases of phlegm-stasis obstruction syndrome, 244 cases of qi and blood deficiency syndrome, and 96 cases of damp-heat blocking collaterals syndrome. According to the X-ray findings, 62 cases were classified as grade 0,265 cases as grade I, 628 cases as grade Ⅱ, 666 cases as grade Ⅲ and 369 cases as grade Ⅳ. ②There was a weak correlation between the distribution of KOA TCM syndromes and gender (χ2=118.8, P＜0.001, C=0.237). More women patients had cold-dampness obstruction syndrome and qi and blood deficiency syndrome, more men patients had damp-heat blocking collaterals syndrome, and the proportion of men and women in patients with phlegm-stasis obstruction syndrome and liver and kidney deficiency syndrome was equivalent. ③There was a weak correlation between the distribution of KOA TCM syndromes and age (χ2=361.3, P＜0.001, C=0.392). Patients with cold-dampness obstruction syndrome and phlegm-stasis obstruction syndrome were mostly aged between 55 and 75 years old. Patients with damp-heat blocking collaterals were mostly under 65 years old. Patients with liver and kidney deficiency syndrome and qi and blood deficiency syndrome were mostly over 55 years old. ④There was a weak correlation between the distribution of KOA TCM syndromes and disease course (χ2=190.7, P＜0.001, C=0.296). The disease course of patients with damp-heat blocking collaterals syndrome was mostly within 6 months, that of patients with cold-dampness obstruction syndrome and phlegm-stasis obstruction syndrome was mostly over 7 months, and that of patients with liver and kidney deficiency syndrome and qi and blood deficiency syndrome was often more than 24 months. ⑤There was a weak correlation between the distribution of KOA TCM syndromes and X-ray grading (χ2=544.1,P＜0.001, C=0.463). Based on the X-ray grading system, cold-dampness obstruction syndrome was mostly classified into grade I, Ⅱ and Ⅲ, damp-heat blocking collaterals syndrome was mostly classified into grade I and Ⅱ, liver and kidney deficiency syndrome and phlegm-stasis obstruction syndrome were mostly classified into grade Ⅲ and Ⅳ, and qi and blood deficiency syndrome was classified into grade Ⅱ and above. Conclusion:TCM syndromes of knee osteoarthritis in the middle-aged and elderly people in Shanghai community were ranked from cold-dampness obstruction syndrome, liver and kidney deficiency syndrome, phlegm-stasis obstruction syndrome, qi and blood deficiency syndrome to damp-heat blocking collaterals syndrome in turn. Moreover, the distribution of KOA TCM syndromes has weak correlations with gender, age, course of disease and X-ray grading, which needs further study.关键词：knee osteoarthritis;TCM syndrome;epidemiological investigation;Shanghai;middle-aged and elderly population
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发布时间：2022-08-31 - 摘要：Objective:To investigate the relationship between subchondral bone marrow lesions and articular cartilage injuries in early knee osteoarthritis (OA) patients. MethodsThe knee joints of 67 patients with early knee osteoarthritis (OA group) and 22 healthy volunteers (healthy group) were enrolled for magnetic resonance imaging (MRI) scanning, and T2 values of knee cartilage in different regions were measured by T2 mapping sequence. In OA group, the whole organ magnetic resonance imaging score (WORMS) was used to evaluate the subchondral bone marrow lesions (BMLs) in different regions. The difference of cartilage T2 values in different regions was compared, and the correlation between cartilage T2 values in different regions and BMLs scores in corresponding regions was analyzed. Results:①In OA group, 41.77% of the patients were within K-L grade I and 58.20% in K-L grade Ⅱ. There was no significant difference in body mass and BMI between the two groups (P>0.05). ②The T2 values of patellar cartilage and femoral cartilage in OA group were significantly higher than those in healthy group (P<0.05). ③Patients with early knee osteoarthritis were often accompanied by different degrees of cartilage injuries and osteophyte formation with high incidences of subchondral bone marrow lesions and meniscus injuries, accounting for 86.4% and 84.8% respectively. It is suggested that the pathological change of early knee osteoarthritis is the degeneration of total knee joints including cartilage, subchondral bone, and meniscus, etc. ④WORMS scores of BMLs in medial and lateral femoral condyle and medial and lateral tibial plateau of patients with early knee osteoarthritis were positively correlated with T2 values of cartilage in corresponding regions (P<0.05). Conclusion:Femoral and patellar cartilages of patients with early knee osteoarthritis have degeneration to varying degrees. The larger the BMLs range in tibial plateau and femoral condyle region, the more severe the cartilage injuries in the corresponding region.关键词：knee osteoarthritis;magnetic resonance;BMLs;cartilage injury;WORMS;T2 mapping
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发布时间：2022-08-31 - 摘要：Objective:To analyze the intestinal microbiota characteristics in ulcerative colitis (UC) patients with thin white fur (TWF) and yellow greasy fur (YGF), and to explore the relationship between tongue fur and intestinal microbiota in UC patients. MethodsThirty-five UC patients with TWF, 35 UC patients with YGF and 35 healthy persons were included to form UCTWF group, UCYGF group and healthy control (HC) group respectively. Fecal specimens were collected, and 16S rDNA gene sequencing and bioinformatics methods were used to analyze and compare the diversity and abundance of intestinal microbiota in each group. Results:①Compared with HC group, the abundance and diversity of intestinal microbiota decreased significantly (P<0.001) in UCTWF group and UCYGF group, and the number of intestinal microbiota species decreased. Compared with UCTWF group, there was no significant difference in intestinal microbiota abundance in UCYGF group (P>0.05), but the number of intestinal microbiota species decreased. ②At the phylum level: compared with HC group, the intestinal microbiota of UCTWF group and UCYGF group decreased in the content of Firmicutes (P<0.05). Compared with UCTWF group, the intestinal microbiota of UCYGF group increased in the content of Cyanobacteria (P<0.05). ③At the genus level: Compared with HC group, the contents of Escherichia-Shigella and Streptococcus increased (P<0.05), while the contents of Subdoligranulum, Dorea, Ruminococcus and Roseburia decreased (P<0.05) in the intestinal microbiota of UCTWF group; Compared with HC group, the contents of Escherichia-Shigella and Lactobacillus increased (P<0.05), while the contents of Dorea, Subdoligranulum, Ruminococcus, Phascolarctobacterium and Roseburia decreased (P<0.05) in the intestinal microbiota of UCYGF group. Compared with UCTWF group, the content of Streptococcus increased (P<0.05) while the contents of Megasphaera, Alloprevotella, Prevotella and Neisseria decreased (P<0.05) in intestinal microbiota of UCYGF group. ④At the levels of class, order and family, the abundance of f_Prevotellaceae, o_Neisseriales and f_Neisseriaceae in the intestinal microbiota of UCTWF group was significantly higher than that of UCYGF group (LDA>2.0). The abundance of c_Bacilli, o_Lactobacillales, f_Streptococccaceae and f_Peptostreptococccaceae in UCYGF group was significantly higher than that in UCTWF group (LDA>2.0). Conclusion:sThe abundance and diversity of intestinal microbiota in UC patients with thin white fur and yellow greasy fur were significantly lower than those in healthy patients. There are differences in intestinal microbiota composition and abundance between UC patients with thin white fur and UC patients with yellow greasy fur. Pathogenic bacteria increase while bacteria with potential protective effect decrease in the intestinal tract of UC patients with yellow greasy fur. Intestinal microbiota detection can provide valuable information for the modernization of tongue diagnosis.关键词：ulcerative colitis;tongue fur;intestinal microbiota;tangue diagnosis;16S rDNA gene sequencing
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发布时间：2022-08-31 - 摘要：Objective:To study rules of acupuncture point selection for tinnitus treatment in modern literature based on data mining to provide more effective and scientific reference for acupuncture prescriptions for clinical treatment of tinnitus. MethodsThe relevant literatures of acupuncture treatment of tinnitus from January 1,1960 to December 31,2018 were retrieved from databases, and the frequency, meridian tropism, location, characteristics and indications of the selected acupoints were statistically analyzed. Cluster analysis, association rules and neural network model were further used to mine and analyze the rules of acupoint selection. Results:① A total of 220 articles were obtained, including 214 articles written in Chinese and 6 articles in English. ②There were 69 acupoints used as the main points in the research literature. The acupoints selected with a higher frequency of use (over 20%) were Tinghui (GB 2), Tinggong (SI 19), Yifeng (SJ 17), Ermen (SJ 21), Zhongzhu (SJ 3), Fengchi (GB 20) and Baihui (DU 20) in turn. Acupuncture treatment of tinnitus varied according to the syndromes of patients, as shown in the combination of points. For example, the complementary points selected with a higher frequency were Taixi (KI 3), Shenshu (BL 23) and Guanyuan (RN 4) in turn for the syndromes of liver and kidney deficiency (including liver and kidney deficiency, kidney essence deficiency and kidney deficiency).③The acupoints selected for tinnitus treatment mainly located at the head and neck, limbs, back and abdomen, among which, the main points locating at the head,especially around the ears, accounting for 68.91%, while the complementary points accounting for 59.32% mainly located in the lower limbs. ④Most main points selected for tinnitus treatment were on the Sanjiao meridian, followed by Gallbladder meridian and Small Intestine meridian. Most complementary points selected for tinnitus treatment were on the Stomach meridian, followed by Liver meridian and Gallbladder meridian. ⑤Selected acupoints (including main points and complementary points) for tinnitus treatment attached great importance to the application of specific points. The use of specific points accounted for 52.17% of the total, of which Five-Shu points were the most frequently used, and the complementary points were mostly specific points.⑥Accord to the cluster analysis, Tinggong (SI 19), Yifeng (SJ 17) and Tinghui (GB 2) formed the core combination of main points for the treatment of tinnitus. ⑦The analysis of association rules showed that the combination of the two main points with the strongest correlation was Fengchi (GB 20)-Tinghui (GB 2), the combination of the three main points with the strongest correlation was Zhongzhu (SJ 3)-Tinghui (GB 2)-Ermen (SJ 21), and the combination of the four main points with the strongest correlation was Zhongzhu (SJ 3)-Yifeng (SJ 17)-Tinghui (GB 2)-Ermen (SJ 21). ⑧The neural network model indicated that the correlation among the five points of “Tinggong (SI 19), Tinghui (GB 2), Zhongzhu (SJ 3), Yifeng (SJ 17) and Ermen (SJ 21)” was relatively strong. Conclusion:sThe acupuncture prescriptions for the tinnitus treatment often use main points and complementary points together. The main points are mostly local points along the meridians around the ears and the complementary points are often selected along the meridians at the distal part and based on syndrome differentiation. Meanwhile, the selection of specific points is paid special attention to when treating tinnitus with acupuncture. In clinical acupoint selection, satisfactory treating effects can be achieved by combining disease differentiation, meridian differentiation with syndrome differentiation.关键词：tinnitus;acupuncture;acupoints;meridian and collateral;rule of acupoint selection;data mining
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发布时间：2022-08-31 - 摘要：This paper introduces the experience of Professor Liu Jiaxiang, a master of traditional Chinese medicine, in treating gastric cancer after surgery. It is considered that the pathogenesis is weakness of spleen and stomach, injury of both qi and yin, and lingering of pathogenic toxin. The dual deficiency of spleen and stomach can be seen throughout the disease process, and the influence of deficiency of stomach yin on the disease is emphasized. On the basis of “strengthening body resistance and treating cancer”, the method of supplementing qi and nourishing yin is used. Sijunzi Decoction and Yiwei Decoction as the main prescription, which can be modified clinically, can effectively prolong the survival time of patients. Two cases were exemplified to advocate the therapeutic thinking.
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发布时间：2022-08-31 - 摘要：This paper introduces Professor Liu Qiquan’s clinical experience in treating recurrent aphthous ulcers by syndrome differentiation and corresponding treatment. It is considered that the accumulation of turbid toxin is the key pathogenesis. The lingering of turbid toxin damages healthy qi and causes aphthous ulcers to recur. Once the disease becomes chronic it affects collaterals, stagnating qi and blood flow, causing long-term fester and difficult-to-heal wound. During the treatment, it is essential to resolve turbidity and remove toxin. Meanwhile, take the morphology and location of aphthous ulcers into consideration and treat according to syndrome differentiation. As long as we adopt multiple measures simultaneously, treat both internally and externally together with daily care, and combine treatment and adjustment, satisfactory curative effect can be achieved. One cured case is exemplified.关键词：recurrent aphthous ulcer;turbid toxin;supplementing qi;activating blood;auricular point;point-application;famous doctor’s experience;Liu Qiquan
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发布时间：2022-08-31 - 摘要：This article explores the pathogenesis and treatment of high-risk human papilloma virus(HPV)infection in traditional Chinese medicine. It is believed that the disease is primarily caused by the deficiency of vital qi while dampness, heat, stasis and poison are the secondary,and the poison damages Chong and Ren and knots in the uterus. It results in abnormality of color,quality and smell in leucorrhea. The clinical symptoms are complicated,even accompanied by vulvar warts and pruritus. Clinical treatments should be adjusted according to the syndrome,such as clearing heat and removing dampness to treat liver depression and spleen suppression,damp-heat flowing down; strengthening vital qi and removing pathogenic factors to eliminate deficiency and evil lingering,as well as dampness, heat, stasis and poison; internal and external treatment to remove condyloma acuminatum.关键词：human papilloma virus;viral infection;TCM pathogenesis;treatment
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发布时间：2022-08-31 - 摘要：Professor Li Zhidao found that when practicing acupuncture on patients with weak body constitution, non-urgent pain due to long-term disease course, or poor acupuncture tolerance, it is often inappropriate to produce too strong stimulation effect. Instead, good therapeutic effect can be obtained by using the “weak acupuncture sensation method”. The application essentials of “weak needle sensation method” mainly include needling method, needle retention method, and needle withdraw method, etc. By adhering to the idea of “acupoints cannot be separated from meridians”, this method should avoid needling tissues around the acupoints that are easy to cause strong acupuncture sensation. During the needle retention period, no manipulation of needles is allowed, or only needle manipulation with small amplitude, low frequency and short time is allowed. When withdrawing needles, gentle and soft needle withdrawal is adopted.The acupuncture therapy abandons the previous clinical thinking pattern, that is, “the stronger the acupuncture sensation is, the better the curative effect will be”, and provides a new idea of clinical treatment for patients with poor acupuncture tolerance by controlling acupuncture sensation.关键词：weak needle sensation method;needle sensation;acupuncture therapy;clinical efficacy;famous doctor’s experience
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发布时间：2022-08-31 - 摘要：To analyze the characteristics of the prescription of Bentun Decoction in Synopsis of the Golden Chamber.It is put forward that white barks of the sweet and the bitter plum roots in the prescription are the same drug. Based on the analysis of the characteristics of Zhong Jing’s use of Guizi(Ramulus Cinnamomi)in the treatment of emotional diseases, it is considered that the addition of Guizi(Ramulus Cinnamomi) in Bentun Decoction can soothe the liver to dredge meridians, make the adverse-qi downwards,and warm the kidney to relieve depression. Meanwhile, the different use of Bentun Decoction and Chaihu (Radix Bupleuri) prescriptions is discussed. And two proven cases are presented.关键词：Bentun Decoction;Guizhi;Chaihu;white bark of plum root;Synopsis of the Golden Chamber
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical effect of Dengzhan Shengmai Capsule combined with conventional therapy on coronary atherosclerotic heart disease (hereinafter referred to as “coronary heart disease”) with hyperlipidemia (qi deficiency and blood stasis syndrome). MethodsNinety patients with coronary heart disease (CHD) accompanied with hyperlipidemia (qi deficiency and blood stasis syndrome) were randomly divided into control group and observation group, 45 cases in each group. The control group was given basic conventional therapy, while the observation group was also given Dengzhan Shengmai Capsule besides the conventional therapy. The course of treatment was 2 months in both groups. The clinical efficacy was observed and the level changes of serum homocysteine (Hcy), high sensitivity C-reactive protein (hs-CRP), blood lipid and TCM syndrome scores were compared. Results:①The total effective rates of the observation group and the control group were 88.9% and 57.8%, respectively, and the clinical efficacy of the observation group was better than that of the control group (P<0.05). ②The total score of TCM syndromes and the score of each sub-item in the two groups decreased after treatment (P<0.05). The total score of TCM syndromes and the score of each sub-item in the observation group were lower than those in the control group (P<0.05). ③The frequency and duration of angina attacks decreased in both groups after treatment (P<0.05). Compared with the control group after treatment, the observation group had less frequency of angina attacks and shorter duration of angina attacks (P<0.05). ④There were significant differences in levels of Hcy, hs-CRP, TG, LDL-C and HDL-C in the observation group before and after treatment (P<0.05), while there were significant differences in levels of hs-CRP, TG, LDL-C and HDL-C in the control group before and after treatment (P<0.05). After treatment, the differences in levels of Hcy, hs-CRP, TG, LDL-C and HDL-C between two groups were significant (P<0.05). ⑤No significant adverse reactions occurred in both groups. Conclusion:Compared with conventional therapy alone, Dengzhan Shengmai Capsule is effective in treating coronary heart disease with hyperlipidemia (qi deficiency and blood stasis syndrome), which can significantly improve angina symptoms and inflammatory state of patients and regulate blood lipid level.关键词：coronary atherosclerotic heart disease;hyperlipidemia;qi deficiency and blood stasis;Dengzhan Shengmai Capsule
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发布时间：2022-08-31 - 摘要：Objective:To observe the inhibitory effect of Jiawei Jinning formula on postoperative recurrence of ground-glass early lung cancer. MethodsSeventy-two patients with ground-glass early lung cancer were randomly divided into treatment group and control group after operation, 36 cases in each group. The treatment group was administered Jiawei Jinning formula, while the control group was administered 1/20 dose of Jiawei Jinning formula. Both groups were treated continuously for 1 year or until the disease progression occurred to observe response evaluation in solid tumors and immune efficacy, as well as the comparison of the changes of TCM syndrome scores. Results:①During the trail, there were 3 dropout cases in the treatment group and 3 dropout cases in the control group, and 66 cases finally completed the test, 33 cases in each group. ②Response evaluation in solid tumor: within one year after operation, the disease control rates of the treatment group and the control group were 93.94% and 72.73%, respectively. The curative effect of treatment group was better than that of control group (P<0.05). ③Immune efficacy: three months after operation, the effective rates of the treatment group and the control group were 75.76% and 69.70%, respectively. There was no significant difference in immune efficacy between the two groups (P>0.05). Comparisons of the immune efficacy were made between the two groups 6 months, 9 months and 12 months after operation respectively, and each time the treatment group showed better immune efficacy than that of the control group (P<0.05). ④ After treatment, the TCM syndrome scores in the two groups showed a continuous downward trend, and the difference was statistically significant compared with the scores in the previous observation time point (P<0.05). Comparisons of TCM syndrome scores between the two groups 6 months, 9 months and 12 months after operation showed that TCM syndrome scores in the treatment group was lower than those in the control group (P<0.05). Conclusion:Jiawei Jinning formula can inhibit the postoperative recurrence of ground-glass early lung cancer to some extent, contribute to the recovery of immune function and improve TCM syndromes, which is worthy of clinical application.关键词：early lung cancer;ground-glass nodule;Jinning formula;post-operation recurrence;immune function;TCM therapy
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发布时间：2022-08-31 - 摘要：Objective: To observe the clinical efficacy of oral administration of Xiaoyong Prescription combined with external application of Jinhuang Ointment in the treatment of early stage of breast carbuncle during lactation and its effect on milk microflora. MethodsA total of 81 patients with breast carbuncle during lactation at the initial stage were randomly divided into control group (n=27) and treatment group (n=54) according to the proportion of 1∶2. Both groups were treated with Jinhuang Ointment externally. The control group was also given cefdinir capsules orally, while the treatment group was given Xiaoyong Prescription orally in the form of granules (modified according to syndromes). The course of treatment in both groups was 3 days. The clinical efficacy was observed, and the changes of quantitative scores of symptoms and signs of breast carbuncle, the positive rate of milk bacteria and specific bacteria quantity were compared. Results:①The total effective rate of the treatment group and the control group were 90.7% and 74.1% respectively, and the clinical efficacy of the treatment group was better than that of the control group (P<0.05). ②The quantitative score of symptoms and signs of breast carbuncle in both groups decreased after treatment (P<0.05), and the quantitative score of breast carbuncle symptoms and signs in the treatment group was lower than that in the control group after treatment (P<0.05). ③The scores of breast carbuncle symptoms and signs including breast pain, skin redness, size and quantity of lumps, body temperature, percentage of neutrophils (N%) and C-reactive protein (CRP) in the two groups were all lower than those before treatment (P<0.05). After treatment, the differences in the scores of size and quantity of breast lumps between the groups were statistically significant (P<0.05). ④There was no significant difference in the positive rate of milk bacteria between the two groups before and after treatment (P>0.05). After treatment, the positive rate of milk bacteria in the treatment group was lower than that in the control group (P<0.05). ⑤There was no significant difference in the number of streptococcus, staphylococcus and lactobacillus in the milk of the two groups before and after treatment (P>0.05). There was no significant difference in the number of the above bacteria between the two groups before and after treatment (P>0.05). Conclusion:Compared with oral administration of cefdinir combined with external application of Jinhuang Ointment, oral administration of Xiaoyong Prescription combined with external application of Jinhuang Ointment has more satisfactory efficacy on patients with early stage of breast carbuncle during lactation in resolving lumps, and can better reduce the positive rate of bacteria in milk.关键词：breast carbuncle during lactation;acute mastitis;lactation;early stage;Xiaoyong Prescription;Jinhuang Ointment;milk microflora;TCM bacteriostatic
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发布时间：2022-08-31 - 摘要：Objective:To observe the effect of total flavonoids in stigmata maydis(TFSM) on the concentration of serum uric acid(SUA) and other related metabolic indexes as well as insulin resistance(IR) state in rat models of insulin resistance complicated with hyperuricemia(IR-HUA rat models). Methods60 male SD rats were randomly divided into the normal control group(Control group),the IR-HUA model group(IR-HUA group),the pioglitazone positive control group(Pioglitazone group),the benzbromarone positive control group(Benzbromarone group),the TFSM high dose group(TFSM.H group) and the TFSM low dose group(TFSM.L group). The Control group was fed with the ordinary feed and drinking water. The other groups were fed with high energy feed and drinking 10% fructose water for 8 weeks. At the beginning of the 7th week(d43),the Control group and the IR-HUA group were given intragastric administration of 2% Tween 80 CMC-Na, and the other groups were given the corresponding medicine solution once a day for 15 days; 3-4 h after intragastric administration,the Control group was given intragastric administration of 0.5% CMC-Na solution,the other groups were given adenine solution(150 mg/kg body mass) once a day for 15 days. Results:Compared with the Control group, in the IR-HUA group, the Lee’s index, the fraction excretion of uric acid(FEUA), the fasting plasma glucose (FPG) and the HOMA-IR index increased, and the fasting insulin (FINS) increased slightly. Compared with the IR-HUA group, the body length of the Benzbromarone group increased and the Lee’s index decreased; the concentrations of SUA and the urine uric acid(UUA) in the Benzbromarone group, TFSM.H group and TFSM.L group decreased slightly, while the urination volume increased slightly. The total uric acid excretion decreased slightly in the Pioglitazone group, Benzbromarone group, TFSM.H group and TFSM.L group, but FEUA increased slightly in the Pioglitazone group, Bebzbrommarone group and TFSM.L group. And the FPG, FINS, HOMA-IR index of the Pioglitazone group, Benzbromarone group, TFSM.H group and FINS of the TFSM.L group decreased slightly. In addition, the concentration of SUA in the Benzbromarone group and TFSM.H group was correlated with FEUA. Conclusion:In IR-HUA model rats,the increase of SUA concentration was accompanied by the increase of FPG concentration,Lee’s index and HOMA-IR index. The results suggested that the IR-HUA rat model is successful. TFSM,pioglitazone and benzbromarone all showed a certain trend of promoting renal excretion of uric acid,reducing SUA concentration and improving IR state in the model rats. The SUA concentration is closely related to the renal uric acid excretion capacity.关键词：total flavonoids in stigmata maydis;insulin resistance;hyperuricemia;metabolic syndrome;IR-HUA model;rats
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发布时间：2022-08-31 - 摘要：Objective:To study the effect of aconite and cinnamon, two traditional Chinese medicines which have the function of dispelling cold by their warming and heating property, on the function of adrenal cortex. MethodsY1 mouse adrenocortical tumor cells (Y1 cells) were taken as subjects in this experiment. Y1 cells were treated with 0.25 g/L, 1 g/L, 4 g/L, 8 g/L concentration of aconite and cinnamon decoction alcohol precipitation for 36 h or 48 h, and a blank control group and 1 μmol/L FSK group were established. The cell proliferation was detected by MTT, the content of corticosterone secreted by the cells was detected by ELISA, the expression of steroid hormone synthase gene by real-time quantitative PCR (RT-qPCR) and protein expression by Western blot. Results:Compared with the control group, the viability of Y1 cells was inhibited significantly in 0.25 g/L, 1 g/L, 4 g/L and 8 g/L aconite and cinnamon groups(P<0.05). The corticosterone secretion of Y1 cells was decreased significantly in 4 g/L and 8 g/L aconite and cinnamon groups(P＜0.01). Cyp11a1, Cyp21a1 and Cyp11b1 genes expression were inhibited significantly by 0.25 g/L, 1 g/L, 4 g/L and 8 g/L aconite and cinnamon(P＜0.01). However, 8 g/L aconite and cinnamon significantly promoted Star gene expression(P＜0.01). And 4 g/L aconite and cinnamon significantly inhibited CYP11A1, CYP21A2, CYP11B1 proteins expression. Compared with the FSK group, 4 g/L aconite and cinnamon silenced the expression of Star, Cyp11a1, Cyp21a1, Cyp11b1 and could not be induced by FSK (P<0.01). Conclusion:Aconite and cinnamon inhibits adrenocortical function by inhibiting cell viability and down-regulating the expression of steroid hormone synthase.关键词：adrenal cortex;corticosterone;Y1 cells;aconite;cinnamon;Chinese Kidney-tonifying herbs
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发布时间：2022-08-31 - 摘要：Objective:To explore the expression of differentially expressed genes (DEGs) in the hippocampus of insomnia rat model with kidney-not-storing-will type. MethodsTwenty SD rats were randomly divided into two groups:the blank group and the kidney-not-storing-will insomnia group (model group),with 10 rats in each group. The disease model was induced by subcutaneous injection of D-galactose (D-gal) combined with intraperitoneal injection of para-chlorophenylalanine (PCPA)in the kidney-not-storing- will insomnia group and the rats in the blank group were given the same volume of 0.9% NaCl solution. The transcriptome sequencing was performed on the hippocampus of the rats in the two groups,and DEGs were screened after the establishment of the rat model. Moreover,GO enrichment and KEGG pathway analysis of DEGs were carried out,and the expression of significant DEGs was verified by real-time fluorescence quantitative PCR (RT-qPCR). Results:Compared with the blank group,there were 1 628 DEGs in the model group,of which 887 DEGs were up-regulated and 741 DEGs were down-regulated. The DEGs were mainly involved in neuro development,cell development,triglyceride metabolism,ribosomes,oxidative stress and inflammatory factors. The DEGs were closely associated with Parkinson’s disease,Alzheimer’s disease,retrograde endocannabinoid signaling transduction pathway and Huntington’s disease. Compared with the blank group,the expression of fatty acid amide hydrolase (Faah) mRNA on hippocampus was significantly down-regulated (P<0.05),and the expression of DNA topoisomerase II (Top2a) mRNA was down-regulated,but there was no statistically significant difference (P＞0.05). The expression of guanine nucleotide binding protein coding gene 3 subunit (Gnb3) and matrix metalloproteinase (MMP-9) were both significantly up-regulated (P<0.05). Conclusion:The kidney-not-storing-will insomnia was related to nerve,metabolism and inflammation pathways. Faah,Gnb3 and MMP-9 were the DEGs in the hippocampus of rats with kidney-not-storing-will insomnia.关键词：failure ofkidney storing will;insomnia model;transcriptomics;rats
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发布时间：2022-08-31 - 摘要：Objective:To evaluate the effect of pristimerin on the migration and invasion of non-small cell lung cancer(NSCLC)NCI-H1299 cells,and explore the underlying mechanism. MethodsNCI-H1299 cells were treated with different concentrations of pristimerin (0~80 μmol/L). The proliferation of NCI-H1299 cells was detected by cell counting kit (CCK-8) assay, and the non-toxic dose of pristimerin to NCI-H1299 cells was determined, which was used as the follow-up dose for the study of pharmacodynamics and molecular mechanism. The effect of pristimerin on the lateral migration ability of NCI-H1299 cells was evaluated by wound-healing assay; the effect of pristimerin on the longitudinal migration and invasion ability of NCI-H1299 cells was evaluated by transwell migration and invasion assay; the protein and mRNA expression levels of epithelial-mesenchymal related markers were measured by Western blot and real-time quantification PCR assay, respectively. Results:When the concentration of pristimerin was less than 2.5 μmol/L, it had no obvious cytotoxicity to NCI-H1299 cells. Compared with the blank control group, the non-toxic dose of pristimerin (0.5 μmol/L, 1.0 μmol/L and 2.0 μmol/L) could significantly inhibit the lateral migration and longitudinal migration and invasion of NCI-H1299 cells (P<0.01). After treatment with pristimerin, the expression levels of epithelial markers E-cadherin and ZO1 were significantly increased, while the expression levels of mesenchymal cell markers N-caherin and Vimentin were significantly decreased (P< 0.01). Conclusion: Pristimerin can inhibit the migration and invasion of NCI-H1299 cells, and this effect may be related to the regulation of epithelial-mesenchymal transformation of NCI-H1299 cells.关键词：pristimerin;non-small cell lung cancer(NSCLC);cancer cells migration;cancer cells invasion;epithelial-mesenchymal transformation
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发布时间：2022-08-31 - 摘要：Objective:To observe the protective effect of Jianpi Jiedu Formula(JPJDF)on 5-FU-induced intestinal mucositis in rats through inhibiting TLRs/NF-κB signaling pathway. MethodsSixty SD rats were randomly divided into blank group, model group, JPJDF-Low(0.31 g/mL), JPJDF-Medium(0.62 g/mL) and JPJDF-High(1.24 g/mL) groups of(0.31 g/mL, 0.62 g/mL, 1.24 g/mL)and Bifico group, with 10 rats in each group. All the rats except the rats in the blank group were injected intraperitoneally with 5-FU (50 mg/kg) once a day for 5 consecutive days. Rats in Chinese medicine treatment groups were given low, middle and high dose of JPJDF(3.1 g/kg, 6.2 g/kg, 12.4 g/kg)respectively, once a day for 7 days during the chemotherapy, while those in Bifico group were given Bifico (151 mg/kg)daily for 7 days. Diarrhea and body weight of rats were recorded daily. Seven days later, HE staining was used to observe the structure of rat small intestine. Immunohistochemistry was used to detect the expression of Occludin protein in small intestine.The serum levels of TNF-α and IL-10 were measured by ELISA, and the expressions of MyD88, p65 and TLRs in the intestine were detected by Western blot. Results:①During the experiment, the weight of the rats in the blank group increased progressively. From the 3rd day, the weight of the rats in the model, JPJDF-Low, JPJDF-Medium, JPJDF-High and Bifico groups began to decrease. From the 5th day of the experiment, the rats in the JPJDF-High group had milder weight loss compared with those in the model group(P<0.05). Diarrhea occurred in all groups except the blank group from the 5th day and reached the peak on the 6th day, and the incidence of diarrhea in the JPJDF-High group was lower than that in the model group on the 6th day (P<0.05).②Compared with the blank group, the expression of Occludin protein in the model group decreased(P<0.01). Compared with the model group, the expression of Occludin protein in all dose groups of JPJDF increased, among which the JPJDF-High group increased most significantly(P<0.01).③The expression of serum TNF-α in the model group was higher than that in the blank group(P<0.05). Each treatment lowered its level and the TNF-α in the groups of JPJDF-Medium and JPJDF-High was lower than that in the JPJDF-Low group(P<0.05). IL-10 in the model group was lower than that in the blank group(P<0.05), and its level in the JPJDF-High group and Bifico group were higher than that in the model group(P<0.05). ④The expressions of MyD88, p65, TLR4 protein in model group was higher than those in the blank group(P<0.01), while the expressions in the JPJDF-Medium and JPJDF-High groups and Bifico group were lower than those in the model group, and the expressions also decreased in the JPJDF-Medium and JPJDF-High groups compared with JPJDF-Low group. Conclusion:JPJDF can inhibit TLRs/NF-κB signaling pathway and regulate inflammatory related factors, finally alleviating the 5-FU-induced intestinal mucosal inflammation in rats.关键词：intestinal mucositis;rats;5-fluorouracil;Jianpi Jiedu Formula;signaling pathways
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发布时间：2022-08-31 - 摘要：This article reviews the current distribution of TCM syndromes in Alzheimer’s disease(AD),and summarizes and discusses the standardization of diagnostic criteria for TCM syndromes in AD. The clinical manifestations of AD are mostly complex syndromes of deficient syndrome with excessive symptoms.Deficient syndrome may include kidney deficiency,heart deficiency,etc.Excessive syndrome may include phlegm turbidity,blood stasis, etc. At present,based on standardized TCM syndrome factors,the AD syndrome scale with TCM characteristics has been used in the clinic.The biological research progress of AD can provide scientific basis for microscopic differentiation of TCM syndromes,and provide research strategies for interpreting AD TCM syndrome theory.The biological research of AD will help the organic combination of macro-syndrome and micro-syndrome differentiation,and formulate the diagnostic criteria of syndromes that are compatible with the entire pathological development of AD.关键词：Alzheimer’s disease;TCM syndromes;diagnostic criteria;review
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发布时间：2022-08-31 - 摘要：This article reviewed the research progress on Baduanjin for depression and anxiety form four aspects:basic connotation,TCM theory,clinical effects and practicing methods.It was found that Baduanjin had favorable effects on primary and secondary depression and anxiety；There would be broad prospect in the application of Baduanjin for depression and anxiety under the background of “Healthy China” strategy.关键词：Baduanjin;depression;anxiety;situation and thinking;review
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发布时间：2022-08-31
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