最新刊期
期 4 ， 2019
- 摘要：This paper introduces Professor Li Xiangyun’s experience in treating infertility due to decreased ovarian reserve function. It is believed that kidney deficiency and blood stasis are the basic pathogenesis of infertility with decreased ovarian reserve function. It is held that invigorating the kidney and activating blood circulation should be the main method for infertility before pregency, while invigorating the kidney and strengthening the spleen be used to prevent abortion after pregnancy. One proven case is presented.关键词：decreased ovarian reserve function;invigorating the kidney and activating blood circulation;infertility;experience of famous doctor;Li Xiangyun
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发布时间：2022-08-31 - 关键词：recurrent oral ulcer;Aphtha ulcer;treatment from Kidney;clinical experience;Shi Xinde
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发布时间：2022-08-31 - 摘要：This paper introduces Professor Liu Xinquan’s clinical experience in treating wet age-related macular degeneration(wAMD) with Xiong Qin Decoction. He thinks that the etiology of wAMD is due to stagnation of qi and blood stasis, mixture of phlegm retention and blood stasis; the therapeutic methods are clearing away heat and activating blood circulation, invigorating the spleen and promoting urination to eliminate dampness; the prescription is modified Xiong Qin Decoction. Three proven cases in the early, middle and late period are presented.关键词：wAMD;Xiong Qin Decoction;clinical experience;Liu Xinquan
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发布时间：2022-08-31 - 摘要：This paper discusses the clinical experience of Professor Li Linggui in treating ulcerative colitis. Professor Li believes that the disease mainly originates from liver depression and spleen deficiency.Spleen deficiency causes dampness and turbidity and qi depression brings about fire and toxicity,which results in internal retention of turbidity and toxicity. This condition may cause the injury of yin,which results in yang nothing to rely on,finally causing the impairment of both yin and yang as well as deficiency of spleen and kidney yang. The main treatment method is removing turbidity and detoxification. The method of eliminating pathogenic factors and treating the branch should be applied in the attack stage and strengthening healthy qi and consolidating the root in the remission stage. At the same time, the method of protecting the stomach and treating both inside and outside should be considered. One proven case is presented.关键词：turbid toxin;ulcerative colitis;experience of famous doctors;Li Diangui
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发布时间：2022-08-31 -
- 摘要：This paper summarizes and analyzes the status of in vivo and in vitro research on lung cancer CTCs, organ-like models, lung cancer prevention models, lung cancer in situ models, lung cancer metastasis models and so on. It is considered that the existing animal models and cell models of lung cancer cannot simulate the actual pathological process of lung cancer, so it is difficult to achieve objective evaluation of intervention factors, and it is more difficult to reflect the overall view of TCM and the advantages of focusing on the tumor growth environment. In order to simulate the actual pathological process of lung cancer and reflect the systemic characteristics of lung cancer, the paper establishes and optimizes the existing research platform in vivo and in vitro based on the academic viewpoint of “hidden toxicity due to vital qi deficiency”, thus optimizes the preclinical research platform of lung cancer.关键词：lung cancer;hidden toxicity due to vital qi deficiency;circulating tumor cell;disease model
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发布时间：2022-08-31 - 摘要：Objective:To study the regularity of syndrome-treatment and prescription-drugs of proteinuria in chronic nephritis in contemporary Chinese medicine using data mining technology. MethodsLiteratures published in recent 20 years about proteinuria in chronic nephritis in contemporary Chinese medicine were retrieved and screened in the database of China National Knowledge Infrastructure (www.CNKI.net) and Wanfang Medical Network (http://med.wanfangdata.com.cn/), and the information on syndrome-treatment and prescription-drugs was extracted to establish a database. In statistical software, frequency statistics, association rules analysis, cluster analysis and other methods were used for data mining to explore the relevant regularity. Results:①The traditional Chinese medicine (TCM) syndromes from high to low frequency were spleen and kidney deficiency, qi and yin deficiency, external attack of wind pathogen, liver and kidney yin deficiency, excessive dampness and heat, etc. ②In terms of the function of prescriptions, tonifying recipes showed the highest frequency of occurrence, 252 times; followed by dampness dispelling recipes, 128 times; and stasis removing recipes, 51 times. ③In terms of the frequency of prescription application, the top five prescriptions are Shenling Baizhu Powder (30 times), Liuwei Dihuang Pill (29 times), Shenqi Dihuang Decoction (20 times), Erzhi Pill (18 times) and Jinkui Shenqi Pill (18 times). ④Astragalus root is the most frequently used traditional Chinese medicine. ⑤After cluster analysis of data on traditional Chinese medicine, five new drug combinations were found, including “poria, rehmannia root, cornus fruit, dioscorea root, alisma tuber, tree peony bark, astragalus root and ovate atractylodes root”, “codonopsis root, dodder seed, aged tangerine peel, licorice root, cherokee rose fruit and euryale seed” and “salvia root, motherwort, Chinese angelica, Sichuan lovage root, red peony root, peach kernel and safflower”. ⑥The core Chinese medicines for proteinuria in chronic nephritis were mainly qi-tonifying and spleen-invigorating drugs (astragalus root, ovate atractylodes root and poria) and drugs supplementing the kidney and astringing essence (cornus fruit, dioscorea root, rehmannia root and euryale seed). Conclusion:①According to TCM, the basic pathogenesis of proteinuria in chronic nephritis is spleen and kidney deficiency. Wind pathogen, dampness-heat and blood stasis are the common pathological factors. Clinically, syndrome differentiation and treatment can be carried out considering six syndromes:spleen and kidney deficiency, qi and yin deficiency, liver and kidney yin deficiency, external attack of wind pathogen, excessive dampness and heat and blood stasis. ②Shenling Baizhu Powder, Liuwei Dihuang Pill and Shenqi Dihuang Decoction are the commonly used prescriptions clinically; the core Chinese medicines for proteinuria in chronic nephritis are mainly qi-tonifying and spleen-invigorating drugs (astragalus root, ovate atractylodes root and poria) and drugs supplementing the kidney and astringing essence (cornus fruit, dioscorea root, rehmannia root and euryale seed).关键词：proteinuria;chronic nephritis;data mining;astragalus root;spleen and kidney deficiency;tonifying qi and invigorating the spleen;supplementing the kidney and astringing essence
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发布时间：2022-08-31 - 摘要：This paper introduces the clinical experience of using syndrome differentiation of six meridians to treat female pelvic inflammatory disease by way of some proven cases, including Guizhi Decoction combined with Danggui Shaoyao Powder for treating pelvic inflammatory disease with the pattern of taiyang-taiyin complication,Taohe Chengqi Decoction for treating the pattern of taiyang blood accumulation ,modified Dachengqi Decoction for the excess pattern of yang ming fu-organ,Chaihu Guizhi Ganjiang Decoction combined with Danggui Shaoyao Powder for the pattern of shaoyang-taiyin complication,modified Zhenwu Decoction for the cold transformation pattern of Shaoyin, Zhuling Decoction for the heat transformation pattern of Shaoyin,and Yiyi Fuzi Baijiangcao Powder and Guizhi Fuling Pill for the pattern of cold and heat complex. It is believed that the treatment of pelvic inflammatory disease guided by the syndrome differentiation of six meridians is more effective than viscera syndrome differentiation in dealing with complicated clinical symptoms.关键词：syndrome differentiation of six meridians;pelvic inflammatory disease;classical prescription;clinical experience
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发布时间：2022-08-31 - 摘要：This paper discussions Huang Liquan’s ideas of the differentiation and treatment as well as his characteristics of medication through three proven cases treated by Huang Liquan. For the cases with exogenous wind-warm influencing liver yang, the treatment should be focused on dredging collaterals and regulating the liver, clearing away heat and resolving phlegm, and the herbs with greasy nature and pungent taste should be less used; for the cases with damp-warmth, the treatment should be concentrated on diathermia with herbs of bland taste; for the cases with pathogenic wind invading upward, the treatment should be considered of suppressing yang with heavy herbs.关键词：Huang Liquan;dredging collaterals and resolving phlegm;eliminating dampness with herbs of bland taste;eliminating wind by suppressing and settling therapies;medical record
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发布时间：2022-08-31 - 摘要：Based on the medical case, this paper introduces the characteristics of the treatment of erectile dysfunction (ED) by Ye Tianshi from six aspects, such as using Wang Jinggong’s Miaoxiang San to regulate heart qi, flesh and blood products to fill the extraordinary vessels, and the herbs with the functions of activating yang and eliminating yin to recover erectile function of the penis, the herbs of promoting interaction between the heart and the kidney to recover penis function, the herbs of soothing the liver and relieve depression to regulate qi and blood, and the herbs of clearing away heat and eliminating dampness to treat the disease.关键词：Ye Tianshi;erectile dysfunction(ED);medical record;characteristics.
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发布时间：2022-08-31 - 摘要：Objective:To explore the clinical efficacy of Qiangxin Jieyu Decoction combined with conventional therapy in the treatment of chronic heart failure complicated with depression in the elderly. MethodsSixty elderly patients with chronic heart failure complicated with depression were randomly assigned into treatment group and control group, 30 patients in each group. Patients in both groups were given the routine treatment for heart failure. On top of this, patients in the control group were treated with sertraline, and those in the treatment group were given Qiangxin Jieyu Decoction. The treatment duration was 8 weeks in both groups. The treatment effect on depression was evaluated. Lee`s heart failure score, total score on Hamilton Depression Scale (HAMD), plasma NT-proBNP level and left ventricular ejection fraction (LVEF) were observed. Results:①=1\*GB3The total effective rate in depression treatment was 93.3% and 83.3% in the treatment and control groups, respectively; and there was statistically significant difference in the treatment effect on depression between the groups (P<0.05). ②=2\*GB3After treatment, the total HAMD-24 score and Lee`s heart failure score decreased (P<0.05), the LVEF level increased (P<0.05), and the NT-proBNP level decreased (P<0.05) significantly in both groups; and there were statistically significant differences in these indicators between the groups (P<0.05). Conclusion:Qiangxin Jieyu Decoction is effective in the treatment of chronic heart failure complicated with depression in the elderly. It can effectively control the patients’ depressive symptoms and improve heart function.关键词：chronic heart failure in the elderly;depression;integrated traditional Chinese and Western medicine therapy;Qiangxin Jieyu Decoction;clinical observation
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发布时间：2022-08-31 - 摘要：Objective:To observe the role of fast-rehabilitation pedal in integrated traditional Chinese and Western medicine therapy in preventing postoperative venous thrombosis of the lower extremities in patients with laparoscopic-assisted transabdominal perineal radical resection for rectal cancer. Methods160 patients after laparoscopic-assisted transabdominal perineal radical resection for rectal cancer were randomly assigned into treatment group and control group, 80 patients in each group. To prevent venous thrombosis of the lower extremities, patients in the control group were treated with intermittent pneumatic pressure pump and those in the treatment group were treated with fast-rehabilitation pedal. Both groups were treated for 7 days. The incidence of venous thrombosis and adverse reactions in the lower extremities was observed. The internal diameter of the common femoral vein (DV), maximum flow rate of the common femoral vein at the end of expiration (Vmax), internal diameter of the common femoral vein/internal diameter of the common femoral artery (DV/DA), D-dimer (D-D) and prothrombin time (PT) were compared. Results:①The incidence of venous thrombosis in the lower extremities was significantly lower in the treatment group (15.00%) than in the control group (32.50%), with statistically significant difference (P=0.009<0.05). ②After treatment, the levels of DV and DV/DA were significantly lower in the treatment group than in the control group (P=0.027,0.000<0.05). The Vmax level was significantly higher in the treatment group than in the control group (P=0.014<0.05).③After treatment, the levels of D-D and PT were significantly lower in the treatment group than in the control group (P=0.000<0.05). ④There was no significant difference in the incidence of adverse reactions between the groups (P=0.311>0.05). Conclusion:Fast-rehabilitation pedal can effectively reduce the incidence of postoperative lower extremity venous thrombosis in patients with laparoscopic-assisted transabdominal perineal radical resection for rectal cancer, improve coagulation function and physiological index of the common femoral vein, and has good safety.关键词：laparoscopic-assisted transabdominal perineal radical resection for rectal cancer;accelerated rehabilitation surgery;lower extremity venous thrombosis;integrated traditional Chinese and Western medicine therapy;acupoint application;prevention and treatment
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发布时间：2022-08-31 - 摘要：Objective:To observe the effect of Jianpi Xiaozhuo Decoction on serum hypersensitive C-reactive protein (hs-CRP) in patients with metabolic syndrome of the phlegm-stasis type. Methods64 patients with metabolic syndrome of phlegm-stasis type were randomly assigned into control group (32 patients) and treatment group (32 patients). Improved lifestyle and exercise therapy were given to the control group and Jianpi Xiaozhuo Decoction was administered to patients in the treatment group on top of the treatment in the control group. Treatment duration was 3 months. Clinical efficacy was observed. Changes in TCM syndrome score, waist circumference and serum hs-CRP level were compared. Results:①=1\*GB3The total effective rate was 84.4% and 50.0% in the treatment and control groups, respectively. The clinical efficacy in the treatment group was superior to that in the control group (P<0.05). ②=2\*GB3After treatment, TCM syndrome scores and waist circumference decreased in both groups (P<0.05); they were lower in the treatment group than in the control group (P<0.05). The differences in TCM syndrome scores and waist circumference were greater in the treatment group than in the control group compared to those before treatment (P<0.05). ③=3\*GB3After treatment, the level of serum hs-CRP decreased in both groups (P<0.05); it was lower in the treatment group than in the control group (P<0.05). The difference in hs-CRP level was greater in the treatment group than in the control group (P<0.05). ④=4\*GB3Pearson correlation analysis showed that the degree of decline in serum hs-CRP level was weakly positively correlated with decrease in waist circumference (r=0.410, P=0.016) and TCM syndrome score (r=0.492, P=0.024). Conclusion:Jianpi Xiaozhuo Decoction shows satisfactory effect in treating metabolic syndrome of the phlegm stasis type. It can significantly improve the clinical symptoms of patients and reduce low-grade in flammatory reactions.关键词：metabolic syndrome;phlegm-stasis type;Jianpi Xiaozhuo Decoction;hypersensitive C-reactive protein
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of YANG’s pricking-cupping therapy combined with electro-acupuncture (EA) in treating degenerative lumbar spinal stenosis (DLSS) of the wind-cold obstruction type. Methods60 patients with DLSS were randomized into treatment group and control group, 30 patients in each group. Patients in the treatment group were treated with YANG’s pricking-cupping therapy combined with EA and those in the control group were given transcutaneous electrical nerve stimulation (TENS) combined with physiotherapy. 8 weeks were regarded as a course of treatment. The clinical efficacy was observed. Changes in scores of simplified Chinese versions of Swiss Spinal Stenosis (SC-SSS) Questionnaire were compared. Results:①During the trial, there was one drop-out in the treatment group, and 59 patients completed the trial. ②The total effective rate was 89.66% and 80.00% in the treatment and control groups, respectively, with no significant difference between the two groups (P>0.05), Clinical efficacy in the treatment group was superior to that in the control group (P<0.05). ③There were statistically significant differences in severity of symptoms and physical function score between 4 and 8 weeks of treatment, with significant decrease in the treatment group compared to those in the control group (P<0.05). Conclusion:Compared with physiotherapy alone, YANG’s pricking-cupping therapy combined with electro- acupuncture shows better efficacy in treating DLSS of the wind-cold obstruction type, significantly relieving pain of the waist and legs, improving intermittent claudication and alleviating numbness of the legs.关键词：degenerative lumbar spinal stenosis;syndrome of wind-cold obstruction;pricking blood therapy;cupping;electro-acupuncture;Dr. YANG Yongxuan
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发布时间：2022-08-31 - 摘要：Objective:To observe the effect of acupuncture combined with Western medicine on serum T lymphocyte subsets, inflammatory factors and vascular cell adhesion molecule-1 (VACM-1) in patients with moderate to severe intermittent allergic rhinitis. Methods120 patients with moderate to severe intermittent allergic rhinitis were randomly assigned into control group and treatment group, 60 patients in each group. The control group was treated with fluticasone propionate nasal spray and azostatin hydrochloride tablets, and the treatment group was given acupuncture on top of treatment in the control group. Treatment duration was 28 days. Clinical efficacy was observed and changes in TCM syndrome score, serum T lymphocyte subsets, inflammatory factors [interleukin-4 (IL-4) and interleukin-10 (IL-10)] and VACM-1 levels were compared. Results:①The total effective rate was 95.0% and 81.7% in the treatment and control groups, respectively. The clinical efficacy in the treatment group was superior to that in the control group (P<0.05). ②At 7 days after treatment, the TCM syndromes in both groups (stuffy nose, intolerably itchy nose, runny nose and frequent sneezing) were improved compared to those before treatment. There was better improvement in the treatment group than in the control group at 7 days after treatment (P<0.05). ③At 7 days after treatment, in both groups, the levels of serum CD3+, CD4+, CD4+/CD8+ increased compared to those before treatment (P<0.05), while the levels of serum CD8+, IL-4, IL-10 and VCAM-1 were lower than those before treatment (P<0.05). There were significant differences in the above indicators between the groups at 7 days after treatment (P<0.05). Conclusion:Acupuncture combined with Western medicine shows satisfactory effect in the treatment of moderate to severe intermittent allergic rhinitis. It can effectively alleviate the clinical symptoms of patients, improve the immune status of the body and reduce inflammatory reactions.关键词：moderate to severe intermittent allergic rhinitis;acupuncture;T lymphocyte subsets;interleukin-4;interleukin-10;vascular cell adhesion molecule-1
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发布时间：2022-08-31 - 摘要：Objective:To observe the effect of Luqi Granule on cardiac function and serum endotoxin (lipopolysaccharid, LPS) level in rats with acute myocardial infarction (MI). Methods60 healthy adult male Wistar rats were randomly assigned into sham operation group, model group and vancomycin group (0.075 g·300 ml-1·d-1), low-dose Chinese medicine group (0.25 ml·kg-1·d-1), middle-dose Chinese medicine group (0.5 ml·kg-1·d-1) and high-dose Chinese medicine group (0.75 ml·kg-1·d-1), 10 rats in each group. Except the sham operation group, MI model was established with rats in the other 5 groups by ligating the left anterior descending coronary artery. The sham operation group received the same procedure except that the coronary artery was not ligated. After successful modeling, each drug group was given the corresponding drug, and the sham operation group and the model group were given an equal volume of 0.9% NaCl solution for 6 weeks. Cardiac function [left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (FS)], serum LPS level and myocardial histopathology were observed. Results:①=1\*GB3Compared with the sham operation group, the model group had lower LVEF and FS values (P<0.05) and a higher serum LPS level (P<0.05). ②=2\*GB3Compared with the model group, the serum LPS level decreased in the vancomycin group and the middle- and high-dose Chinese medicine groups (P<0.05); the LVEF and FS values increased in the middle- and high-dose Chinese medicine groups (P<0.05). ③=3\*GB3Compared with the vancomycin group, the LVEF and FS values increased in the middle- and high-dose Chinese medicine groups (P<0.05). ④=4\*GB3Compared with the low-dose Chinese medicine group, the LVEF and FS values increased and the serum LPS level decreased in the middle- and high-dose Chinese medicine groups (P<0.05). ⑤=5\*GB3There were no significant differences in cardiac function and serum LPS level between the middle- and high-dose Chinese medicine groups (P>0.05). Conclusion:Luqi granule improves the cardiac function of rats with acute MI and reduces inflammatory area of myocardial tissue. The mechanism may be related to decrease in serum LPS level and maintenance of cardiac homeostasis.关键词：acute myocardial infarction;rats;Luqi Granule;cardiac function;Endotoxin
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发布时间：2022-08-31 - 摘要：Objective:To observe the effect of gastrodine on p-AKT and caspase 3 in rats with epilepsy induced by pentylenetetrazole (PTZ). Methods69 Wistar rats were assigned into blank control group (18 rats), epilepsy model group (PTZ group, pentylenetetrazole, 55~75 mg/kg) (28 rats) and gastrodine group (Gas group, gastrodine, 200 mg/kg) (23 rats). Epileptic model was established with rats in all of groups by PTZ intraperitoneal injection except the blank control group. Gastrodine was given to the Gas group. Histomorphological changes of hippocampus were observed under electron microscope. The expression of serine-threonine protein kinase (p-AKT) and cysteine-aspartic acid specific protease 3 (caspase 3) in hippocampus was detected by immunohistochemistry and Western blotting. Results:Compared with the control group, the hippocampal area of the PTZ group showed remarkable pathological changes of epilepsy, including significant neuronal degeneration and necrosis, mainly the large pyramidal cells and some dentate gyrus granulosa cells, nucleus condensation in damaged cells, fragmentation-like nuclear changes, cytosolic swelling, disappearance of the nissl bodies and increase in cell peripheral gap and cell volume. The pathological changes were improved after drug intervention. After seizure in rats, the expression of p-AKT and caspase-3 was higher in the epilepsy model group than in the blank control group (P<0.05). Compared with the epilepsy model group, the expression of caspase-3 decreased in the gastrodine group (P<0.05), with very significant difference at day 3 between the two groups (P<0.01). Conclusion:Gastrodine plays an anti-apoptotic role and protects against epilepsy by reducing the expression of p-AKT and caspase 3.关键词：gastrodine;epilepsy;p-AKT;caspase 3;rats
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发布时间：2022-08-31 - 摘要：Objective:To explore the pharmacological mechanism of Fu-Fang-Li-Shao Pill (FFLSP) improving chronic migraine of the liver-yang hyperactivity type by observing changes in the levels of pro-inflammatory factors in plasma of rats with nitroglycerin-induced chronic migraine. Methods36 male Sprague-Dawley rats were randomly assigned into 6 groups:normal control (NC) group, migraine group, low-dose FFLSP (FFLSP-L) group, middle-dose FFLSP (FFLSP-M) group, high-dose FFLSP (FFLSP-H) group and flunarizine hydrochloride (FH) group. FFLSP at corresponding doses (420,0 and 1,0 mg/kg) was given to the FFLSP-L, FFLSP-M and FFLSP-H groups; FH (1 mg/kg) was given by gavage to the FH group, and an equal volume of 0.9% NaCl solution was given by gavage to the NC group and the migraine group. Each group was dosed once a day for 30 days. Except the NC group, migraine model was established by subcutaneous injection of nitroglycerin (10 mg/kg) to rats in the other 5 groups on days 18,1, 24,7 and 30 during treatment. The gene and protein levels of pro-inflammatory factors and 5-HT in plasma of rats in each group were detected with qRT-PCR, ELISA and Griess reagent. Results:①Compared with the NC group, the expression of iNOS, IL-1β, IL-6 and TNF-α mRNA in plasma increased significantly in the migraine group (P<0.001); the content of iNOS, IL-1β, IL-6 and TNF-α mRNA in plasma decreased significantly after FFLSP treatment (P<0.001). ②Compared with the NC group, the levels of IL-1β, IL-6, TNF-α and NO products in serum increased significantly in the migraine group (P<0.05); and they were reduced significantly after FFLSP treatment (P<0.05). ③Compared with the NC group, the level of 5-HT in serum decreased in the migraine group (P<0.05); and it increased significantly after FFLSP treatment (P<0.05). Conclusion:FFLSP may reduce inflammatory reactions and relieve migraine of the liver-yang hyperactivity type by reducing the gene and protein levels of pro-inflammatory factors in the blood.关键词：migraine;Fu-Fang-Li-Shao Pill;pro-inflammatory factors;5-HT;rats
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发布时间：2022-08-31 - 摘要：Objective:To explore the effects of Bushen Gubiao Granules on serum interleukin-2 (IL-2), interferon-γ (IFN-γ) and tumor necrosis factor (TNF-α), NK cell activity and splenic lymphocyte proliferation in hypoimmune mice. MethodsSixty male ICR mice were randomly assigned into 6 groups:blank control group, model group, Yupingfeng group, and high-, medium- and low-dose Bushen Gubiao Granules groups. Except the blank control group, mice in the other groups were injected cyclophosphamide 50 mg·kg-1·d-1 intraperitoneally for 3 days to establish an animal model of immunosuppression. Corresponding drug was given by gavage to each group for 28 days. Splenic lymphocyte proliferation was detected by MTT colorimetry; the killing activity of NK cells was determined using LDH method; and the serum levels of IL-2 and IFN-γ were measured by ELISA. Results:Compared with the model group, Bushen Gubiao Granules could enhance the proliferation of splenic lymphocytes in immunosuppressed mice (P<0.05), increase the killing rate of these cells (P<0.001), and significantly increase the serum levels of IL-2, IFN-γ and TNF-α (P<0.01). Conclusion:Bushen Gubiao Granules can enhance the transformation and killing ability of splenic lymphocytes, and increase the serum levels of IL-2, IFN-γ and TNF-α in immunocompromised mice, thereby improving the immune function of the body.关键词：Bushen Gubiao Granules;interleukin-2;interferon-γ;NK cell;splenic lymphocyte proliferation;mice
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发布时间：2022-08-31 - 摘要：Objective:To investigate the effect of hydroxycamptothecin on proliferation and energy metabolism of human prostate cancer PC-3 cells and on the critical enzymes in these cells. MethodsUsing MTT method, the proliferation inhibitory ability was determined after the PC-3 cells were treated with hydroxycamptothecin at 1.0,2.0 and 5.0 μmol/L for 24h. The energy metabolism parameters related to glucose uptake, lactic acid production and ATP production were determined with spectrophotometry. The expression of lactate dehydrogenase A (LDHA) and AMP-activated protein kinase (AMPK) was detected by Western blotting. Results:Hydroxycamptothecin inhibited proliferation of the PC-3 cells at 1.0,2.0 and 5.0 μmol/L in a dose-dependent manner (P<0.05, P<0.01), with an IC50 value of 3.24 μmol/L. Compared with the control group, hydroxycamptothecin at different concentrations could reduce glucose uptake, inhibit lactic acid production and ATP production in a dose-dependent manner (P<0.05, P<0.01). Western blotting results showed that hydroxycamptothecin at different concentrations could reduce the expression of LDHA and AMPK in PC-3 cells (P<0.05, P<0.01). Conclusion:Hydroxycamptothecin inhibits aerobic glycolysis and cell proliferation in the tumor by reducing the expression of LDHA and AMPK in PC-3 cells.关键词：hydroxycamptothecin;PC-3 cells;lactate dehydrogenase A (LDHA);AMP-activated protein kinase (AMPK)
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发布时间：2022-08-31 - 摘要：Objective:To investigate the chemical components of the dry fruits of Forsythia suspense(Thunb.)Vahl and their anti-inflammatory activities. MethodsChemical compounds were isolated from the dry fruits of Forsythia suspense using silica gel, Sephadex LH-20 and MCI chromatography, in combination with isolation techniques including ODS middle-pressure preparative chromatography, preparative thin layer chromatography and preparative liquid chromatography, and structures of the components were identified with mass spectrometry and nuclear magnetic resonance spectroscopy. The inhibitory effect of some components on nitrix oxide(NO)secretion were determined by using induced model on mouse macrophages RAW 264.7. Results:Thirty compounds were isolated and the compounds including 4-allylcatechol (1), 19-hydroxy-13-oxo-15,6-dinor-ent-labda-8 (17)-ene (2), 18-hydroxy-13-oxo-15,6-dinor-cleroda-3,4-ene (3), 3,3-oxo-15,6-dinor-ent-labda-8 (17)-ene (4), dammarendiol II (6), serralabdane B (9), and (4S, 9R, 10R) methyl 18-carboxy-labda-8,3 (E)-diene-15-oate (15) were isolated for the first time from genus Forsythia. Experimental results of the cell inflammation model showed that the compounds 2~4, (±)-pinoresinol (16) and rengyolone (18) inhibited NO secretion. Conclusion:8 diterpenoids were isolated from the dry fruits of Forsythia suspense, and anti-inflammatory activities of compounds 2~4 were reported for the first time.关键词：Forsythia suspense;chemical components;nitric oxide;anti-inflammatory
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发布时间：2022-08-31 - 摘要：This paper reviews the research progress on effect of traditional Chinese medicine on eosinophils in bronchial asthma. Eosinophils are important effector cells in asthma; traditional Chinese medicine can reduce eosinophil-related airway inflammation, inhibit eosinophil-related airway remodeling, and regulate eosinophil-related immune response.关键词：traditional Chinese medicine;bronchial asthma;eosinophils;airway inflammation;airway remodeling;immune regulation;review
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发布时间：2022-08-31 - 摘要：This paper reviews the research progress on the mechanism of traditional Chinese medicine regulating immune cells in the treatment of bronchial asthma. Bronchial asthma is mainly characterized by chronic airway inflammation, and its pathogenesis involves a variety of immune cells (eosinophils, neutrophils, helper T cells, mast cells, dendritic cells and innate lymphocytes, etc.) and corresponding cytokines. Compound Chinese traditional medicine (Pingchuan Formula and Shegan Mahuang Chonglou Decoction etc.) and Chinese medicine extract (astragaloside and astragalus polysaccharide etc.) relieve airway inflammation and airway remodeling state in bronchial asthma by regulating immune cells.关键词：bronchial asthma;immune cells;pathogenesis;traditional Chinese medicine therapy;review
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发布时间：2022-08-31
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