最新刊期
期 3 ， 2019
- 摘要：This paper introduces Professor Peng Peichu’s clinical experience in the treatment of adolescent’s acne. It is considered that the key to the onset of adolescent’s acne lies in the hyperactivity of kidney fire and damp-heat steaming the head. The principle of treatment is to clear away the kidney fire and remove damp- heat. One proven case is presented.关键词：acne;adolescent;kidney fire and damp-heat;experience of famous doctor;Peng Peichu
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发布时间：2022-08-31 - 摘要：This paper introduces the clinical experience of Lu’s acupuncture and moxibustion in the treatment of diplopia. It is believed that the etiology and pathogenesis of diplopia are external pathogenic factors blocking collaterals, deficiency of qi and blood, deficiency of the liver and kidney, and stagnation of qi and blood stasis. During the treatment, we should pay attention to treatment based on syndrome differentiation and follow the three principles of “local acupoints to dredge local meridian qi”, “distal acupoints to regulate whole body qi and blood” and “selecting corresponding Back-Shu acupoints to regulate the function of zang-fu organs”.关键词：Diplopia;Lu’s acupuncture and moxibustion;clinical experience
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发布时间：2022-08-31 - 关键词：depression syndrome;couplet medicines;clinical experience;Zhang Yunling
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发布时间：2022-08-31 - 摘要：This paper explores the academic experience of Professor Yan Dexin, a master of Chinese medicine, on the treatment of insomnia based on the theory of qi and blood. When treating the disease, Professor Yan Dexin would distinguish whether it was a new disease or a chronic one, a deficiency syndrome or an excess one, and chose prescriptions attached importance to regulating qi, promoting blood circulation and tranquilizing the mind. The basic treatment principle for excess syndrome was to soothe the liver and regulate qi, calm the heart and tranquilize the spirit, and the modified prescriptions with Chaihu(Radix Bupleuri) were often used. For deficiency syndrome, the principle was to strengthen the spleen and replenish qi, nourish blood and tranquilize the spirit, and the prescriptions were mainly the modified Guipi Decoction or Suanzaoren Decoction. With the development of the disease and changes of pathogenesis, some effective medicines were added into the prescriptions for regulating the functions of zang-fu organs and removing pathological products such as phlegm-fire and blood stasis. In addition, He was good at the application of synergistic couplet medicines and powerful tranquilizers, especially at the application of Huanglian(Rhizoma Coptidis) with unique characteristics.关键词：insomnia;treatment based on the theory of qi-blood;experience of famous doctor;Yan Dexin
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发布时间：2022-08-31 -
- 摘要：Objective:To explore the value of collagen fibers deposition, CD34 and Cytokeratin 19(CK19) expression in liver tissue in the diagnosis of chronic hepatitis B with blood stasis syndrome, in order to provide some reference for traditional Chinese medicine(TCM) syndrome differentiation and treatment on chronic hepatitis B.MethodsResearch objects were 128 hepatitis B patients undergoing liver biopsy.According to TCM four diagnostic information, the patients were divided into blood stasis syndrome group and non-blood stasis syndrome group.Hematoxylin-eosin staining, reticular fiber staining, Masson staining, CD34 and CK19 immunohistochemical staining were performed on the liver tissue sections, and then the positive expressions of collagen, CD34 and CK19 were calculated by the image analysis software to observe and compare the changes of collagen fiber, CD34 and CK19 expression in liver tissue. Results:①The positive expressions of collagen fibers, CD34, and CK19 in patients with liver fibrosis stages S2, S3 and S4 were significantly than those in patients with S1 stage(P<0.05). The expression of collagen fibers and CD34 in patients with S4 stage were significantly higher than those in stages S2 and S3(P<0.05). ②The positive area of collagen fibers in G4 degree of inflammatory activity was significantly larger than those in G1, G2 and G3 degree (P<0.05). There was no significant difference between G1, G2 and G3 degree (P>0.05) and no significant difference in positive expression of CD34 and CK19 between different degrees of inflammatory activity (P>0.05).③There was a significant difference in fibrosis stages between patients with blood stasis syndrome and non-bloody stasis syndrome(P<0.05).The positive expression of collagen fiber and CD34 in patients with blood stasis syndrome was significantly higher than that in patients without blood stasis syndrome(P<0.05). Conclusion:Positive expression of collagen fiber and CD34 in liver tissue of patients with chronic hepatitis B can be a quantitative index of liver fibrosis.Collagen fiber deposition and angiogenesis may be the main changes in liver histopathology of chronic hepatitis B with blood stasis syndrome, which may provide some references for TCM syndrome differentiation and treatment on chronic hepatitis B.关键词：chronic hepatitis B;liver histopathology;blood stasis syndrome
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发布时间：2022-08-31 - 摘要：This paper introduces the clinical experience of Professor Li Samei in applying Mahuang Shengma Decoction. Combined with the original text of the Shanghanlun(Treatise on Cold Pathogenic Diseases)and the annotations of ancient and modern doctors, this paper discusses the recipe, and syndromes, etiologies and pathogenesis of the diseases treated with Mahuang Shengma Decoction from the aspect of correspondence between prescription and syndromes. Mahuang Shengma Decoction has the functions of dispersing stagnant yang, clearing away heat of the upper and warming yang of the lower part of the body and harmonizing cold and heat, which is used for the treatment of the spleen yang damage due to incorrect treatment of exterior syndrome by purgation, stagnation of heat in the lung and stomach,invasion of Jueyin by pathogen, and interior stagnation of yang qi. One proven case is presented .关键词：Mahuang Shengma Decoction;Jueyin disease;Shanghanlun(Treatise on Cold Pathogenic Diseases);experience of famous doctor;Li Saimei
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发布时间：2022-08-31 - 摘要：Combined with the etiology, pathogenesis and syndrome characteristics of depression syndrome, this paper summarizes the methods of differentiation and treatment of depression syndrome based on ancient and modern literature. Depression syndrome can be treated from emotion, five zang-organs, yin and yang. It can also be treated by stages, disease differentiation and syndrome differentiation, special method and special prescription, and morphological treatment. Psychotherapy is indispensable in the treatment of depression syndrome. In addition, acupuncture and moxibustion, massage, taijiquan and other methods can play an auxiliary role in the treatment.关键词：depression syndrome;treatment;Chinese medicine
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发布时间：2022-08-31 - 摘要：Xiaokebing (diabetes mellitus) is closely related to diet and there have been a lot of description about food taboos of Xiaokebing since ancient times. The thoughts originated in the Huangdi Neijing (Yellow Emperor’s Inner Canon of Medicine) and the specific discussion about them first occurred in the Shanghɑn Zabinglun (Treatise on Febrile and Miscellaneous Diseases). This theory had been accumulated and perfected in many medical books in the Wei, Jin, Southern and Northern dynasties . By the Tang dynasty, the theory had been more mature and formed its own system in the Waitai Miyao (Medical Secrets of an Official) and Yixinfang(Ishinpo). This paper systematically summarizes the description of food taboos of Xiaokebing in the classical medical books before the Tang dynasty and analyses its inherent laws to help expand and deepen academic research on diabetic diet.关键词：Xiaokebing;diabetes mellitus;dietotherapy;food taboo
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发布时间：2022-08-31 - 摘要：Objective:To evaluate the clinical efficacy of Fuzheng Kangyan Formula combined with gefitinib on non-small cell lung cancer (NSCLC) (stage IV) of the qi deficiency and blood stasis type. Methods60 patients were randomly assigned into control group and treatment group, 30 patients in each group. The control group was given gefitinib + placebo and the treatment group received gefitinib + Fuzheng Kangyan Formula. Drugs were given until tumor progression. The short- and long-term efficacy (progress-free survival, PFS), changes in KPS score and adverse reactions were observed and compared. Results:①There were no drop-outs during the trial. ②The effective rate and tumor control rate were 53.33% and 93.33% respectively in the treatment group and 43.33% and 90.00% respectively in the control group. There was no statistically significant difference in short-term efficacy between the groups (P>0.05). ③There was statistically significant difference in PFS between the groups, which was longer in the treatment group than in the control group (P<0.05). ④After treatment, the KPS score was significantly higher in the treatment group than in the control group (P<0.05). ⑤Severity of adverse reactions including diarrhea, nausea and vomiting was significantly different, which was obviously lower in the treatment group than in the control group (P<0.05).关键词：non-small cell lung cancer;qi deficiency and blood stasis syndrome;Fuzheng Kangyan Formula;gefitinib;molecular targeted agent;progress-free survival
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发布时间：2022-08-31 - 摘要：Objective:To observe the effects of Yiqi Yangyin Formula on radiation pneumonitis(RP) and lung function in patients undergoing postoperative radiotherapy for non-small cell lung cancer (NSCLC) (stage IIIa). Methods60 patients undergoing postoperative radiotherapy for NSCLC (stage IIIa) were randomly assigned into treatment group and control group, 30 patients in each group. The control group received routine radiotherapy after operation, and the treatment group received Yiqi Yangyin Formula in addition. Both groups were treated for 28 days. Changes in classification of acute RP (RTOG classification) and in lung function were observed. Results:①During the trial, there were 1 and 3 drop-outs in the treatment and the control groups respectively; and 56 patients finally completed the trial, including 29 in the treatment group and 27 in the control group.②At 2 months after radiotherapy, there was statistically significant difference in RTOG grade in the treatment group (P<0.05), while there was no statistically significant difference in the control group (P>0.05) compared to that at the end of radiotherapy. At the end of radiotherapy, there was no statistically significant difference in RTOG grade between the groups (P>0.05), and no statistically significant difference in the incidence of RP between the treatment group (72.41%) and the control group (85.19%) (P>0.05). At 2 months after radiotherapy, there was statistically significant difference in RTOG grade between the groups; and in the treatment group, the incidence of RP was significantly lower than in the control group (37.93% vs. 85.19%), and the degree of inflammation was significantly lower than in the control group (P<0.05). ③There was no significant difference in FVC, FEV1 or MVV in the groups at the end of radiotherapy compared to that before radiotherapy (P>0.05). FVC, FEV1 and MVV decreased significantly in the control group(P<0.05) and they remained stable in the treatment group with no statistically significant differences (P>0.05) at 2 months after radiotherapy compared to those before radiotherapy. At the end of radiotherapy, there was no significant difference in FVC, FEV1 or MVV between the groups (P>0.05). At 2 months after radiotherapy, the differences in FVC, FEV1 and MVV were statistically significant between the groups, significantly higher in the treatment group than in the control group (P<0.05). ④Variance analysis of repeated measures data showed that there was statistical significance in the time factor and the time x group factor in intra-group variation of FVC, FEV1 and MVV (P<0.05), and there was no statistical significance in the group factor in inter-group variation (P>0.05). Conclusion:Yiqi Yangyin Formula can stabilize the lung function of patients undergoing postoperative radiotherapy for NSCLC (stage IIIa) to a certain extent, lower the incidence of radiation pneumonitis, and help to reduce the degree of the inflammation.关键词：radiation pneumonitis;lung function;non-small cell lung cancer;Yiqi Yangyin Formula
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发布时间：2022-08-31 - 摘要：Objective:To observe the effects of Wushen Oral Liquid combined with conventional Western medicine therapy on autonomic nervous function in patients after percutaneous coronary intervention (PCI) for myocardial infarction (MI) of the qi-yin deficiency type. Methods70 patients after PCI for MI of the qi-yin deficiency type were randomly assigned into treatment group and control group, 35 patients in each group. Conventional Western medicine therapy was given to the control group and its combination with Wushen Oral Liquid was administered to the treatment group. The treatment course was 3 months in both groups. Therapeutic effect on TCM syndromes was observed and changes in measures of autonomic nervous function (variability and deceleration capacity of heart rate) were compared. Results:①The overall effective rate was 97.1% and 88.6% in the treatment and control groups respectively. The therapeutic effect on TCM syndromes in the treatment group was significantly superior to that in the control group (P<0.05). ②The levels of SDNN, SDANN, rMMSD and PNN50 increased significantly in the treatment group (P<0.05) and the levels of SDNN and rMMSD increased significantly in the control group (P<0.05) after treatment compared to those before treatment. The levels of SDNN, SDANN and rMMSD were significantly higher in the treatment group than in the control group after treatment (P<0.05). ③The deceleration capacity of heart rate increased significantly in both groups after treatment compared to that before treatment (P<0.05). There was no significant difference in deceleration capacity of heart rate between the groups after treatment (P>0.05). Conclusion:Wushen Oral Liquid in combination with conventional Western medicine therapy improves the TCM syndrome of patients after PCI for MI of the qi-yin deficiency type, and balances the autonomic nervous function by increasing the measures for heart rate variability (SDNN, SDANN and rMMSD) and tending to elevate the deceleration capacity of heart rate.关键词：myocardial infarction;percutaneous coronary intervention;qi-yin deficiency;integrated traditional Chinese and Western medicine therapy;Wushen Oral Liquid;autonomic nervous function
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发布时间：2022-08-31 - 摘要：Objective:To study the endometrial thickness, volume, endometrial blood flow pattern and uterine blood flow parameters at midluteal phase in patients with persistent thin endometrium and the effect of Ma’s Bubao Decoction combined with acupuncture on endometrial receptivity (ER) in them. MethodsNinety cases of persistent thin endometrium of childbearing age were randomly divided into treatment group and control group, 45 cases in each group . In addition, 30 women of normal childbearing age in the same period were included as normal control group .The control group was treated with aspirin, while the treatment group was treated with Ma’s Bubao Decoction and acupuncture on the basis of aspirin. Both groups were treated for 2 months (suspend treatment during menstrual period) and then the changes of endometrial thickness, volume, endometrial blood flow pattern, uterine blood flow hemodynamic parameters and TCM syndrome scores at midluteal phase were observed. Results:①During the experiment, there were 3 cases dropped in the treatment group (due to interruption of treatment), 2 cases in the control group, and no drop in the normal control group. Totally 115 cases completed the experiment, 42 cases in the treatment group, 43 cases in the control group and 30 cases in the normal control group. ②At midluteal phase, the thickness and volume of endometrium in the thin endometrium group were significantly lower than those in the normal control group (P < 0.05); the levels of endometrial blood flow S/D and uterine artery blood flow mPI, mRI and mS/D were significantly higher than those in the normal control group (P < 0.05); the difference of endometrial blood flow pattern between the two groups was significant (P < 0.05). ③In the midluteal phase, there were significant differences in endometrial thickness, endometrial volume, endometrial blood flow S/D, uterine artery blood flow mPI and endometrial blood flow pattern compared with those before treatment within both groups (P < 0.05), and there were significant differences in the uterine artery blood flow mS/D in the treatment group (P<0.05). There were significant differences in endometrial thickness, endometrial blood flow S/D and uterine artery mS/D between the two groups after treatment. The improvement of the above indexes in the treatment group was significantly better than that in the control group (P<0.05). There was no significant difference in endometrial blood flow pattern (P>0.05). ④ There were significant differences in TCM syndrome scores compared with those before treatment within both groups (P<0.05). All of them were significantly decreased after treatment. After treatment, the TCM syndrome scores in the treatment group were significantly lower than those in the control group (P<0.05). Conclusion:①The resistance of uterine blood flow at midluteal phase of persistent thin endometrium patients was significantly higher than that of normal women, and the endometrial thickness and volume were significantly decreased. ②On the basis of oral aspirin, the combination of Ma’s Bubao Decoction and acupuncture can improve the clinical symptoms of patients with persistent thin endometrium, increase the thickness and volume of endometrium and enhance the blood flow perfusion of the uterus,which are conducive to the improvement of endometrial receptivity.关键词：thin endometrium;Ma’s Bubao Decoction;acupuncture;endometrial receptivity;endometrial blood low;uterine artery blood flow
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发布时间：2022-08-31 - 摘要：Objective:To investigate the clinical efficacy of sclerosing foam combined with point-stage stripping in the treatment of varicose veins of the lower limbs under local anesthesia by Fushe acupoint injection. Methods100 patients with varicose veins of the lower limbs were randomly assigned into treatment group and control group, 50 patients in each group. The treatment group was given sclerosing foam combined with point-stage stripping under local anesthesia by Fushe acupoint injection and the control group received high ligation of the great saphenous vein combined with stripping under lumbar anesthesia. Regular follow-up was performed for a year after operation. Clinical efficacy and relapse were observed, and intraoperative measures (blood loss and operation duration), duration of hospitalization (days) and postoperative adverse reactions (nausea and vomiting, urinary retention and limb numbness) and complications (wound infection, ecchymosis or hematoma and pain) were compared. Results:①The cure rate was 96.0% and 98.0% in the treatment and control groups, respectively with no significant difference between the groups (P<0.05). ②Blood loss and duration of hospitalization decreased significantly in the treatment group compared to the control group (P<0.05). There was no statistically significant difference in operation duration between the groups (P>0.05). ③Good post-operative recovery was seen in the treatment group, with no patients showing post-operative reactions including nausea, vomiting, urinary retention or limb numbness. Acceptable recovery was seen in the control group, with 10 patients showing the post-operative reactions mentioned above. ④In terms of post-operative complications, there was significant decrease in wound infection, ecchymosis or hematoma and pain in the treatment group than in the control group (P<0.05). ⑤1 and 8 patients had relapse in the treatment and control groups respectively during follow-up, and the relapse rate was significantly lower in the treatment group than in the control group (P<0.05). Conclusion:Compared with high ligation of the great saphenous vein combined with stripping under traditional lumbar anesthesia, sclerosing foam combined with point-stage stripping under local anesthesia by Fushe acupoint injection shows advantages in the treatment of varicose veins, including decreased blood loss, shorter duration of hospitalization, less postoperative complications, decreased wound infections, less pain and lower relapse rate.关键词：Fushe acupoint;injection anesthesia;varicose veins;point-stage stripping;sclerosing foam
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发布时间：2022-08-31 - 摘要：Objective:To observe the effects of Yiqi Huoxue Lishui Daxie Formula combined with western medicine on proliferative vitreoretinopathy in patients after reposition for retinal detachment. Methods60 patients after reposition for retinal detachment were randomly assigned into treatment group and control group, 30 patients in each group. Patients in the control group received tobramycin dexamethasone eye drops and compound tropicamide eye drops, while those in the treatment group received Yiqi Huoxue Lishui Daxie Formula on top of the treatment in the control group for 7 consecutive days after surgery. The treatment course was 21 days in both groups. The clinical efficacy was observed, and changes in the PVR score, electroretinogram and intraocular pressure were compared. Results:①The effective rate was 53.3% and 26.7% at 7 days after surgery, 86.7% and 60.0% at 30 days after surgery, and 96.7% and 83.3% at 60 days after surgery in the treatment and control groups respectively. There were statistically significant differences in clinical efficacy between the groups at each time point of observation (P<0.05). ②The PVR score decreased significantly in each group at 30 and 60 days after surgery compared to that before surgery (P<0.05). It was significantly lower in the treatment group than in the control group at 30 and 60 days after surgery (P<0.05). ③The amplitude and latency of a and b waves on electroretinogram increased at 60 days after surgery compared to those before surgery in each group (P<0.05). There were statistically significant differences in the amplitude and latency of a and b waves between the groups at 60 days after surgery (P<0.05). Conclusion:Yiqi Huoxue Lishui Daxie Formula shows satisfactory clinical efficacy in patients after reposition for retinal detachment, suppresses PVR progression and promotes recovery of visual function.关键词：retinal detachment;after surgery;proliferative vitreoretinopathy;therapy of traditional Chinese medicine;Yiqi Huoxue Lishui Daxie Formula;electroretinogram
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of acupuncture and moxibustion in the treatment of constipation in patients with middle- and advanced-stage Parkinson’s disease (PD) and the effect on medication compliance. Methods72 middle- and advanced-stage PD patients with constipation were randomly assigned into treatment group and control group, 36 patients in each group. Routine treatment for PD was given to patients in both groups, and in addition, patients in the control group took Maren Soft Capsules and those in the treatment group were treated with acupuncture and moxibustion. The treatment course was 28 days in both groups. The clinical efficacy was observed and changes in the score on unified Parkinson’s disease rating scale (UPDRS) II and III, constipation score and medication compliance were compared. Results:①The overall effective rate was 72.2% and 44.4% in the treatment and control groups respectively, and the clinical efficacy in the treatment group was superior to that in the control group (P<0.05). ②Constipation improved significantly in both groups after treatment compared to that before treatment (P<0.05); and it showed significantly greater improvement in the treatment group than in the control group after treatment (P<0.05). ③The UPDRS II score decreased in both groups after treatment compared to that before treatment (P<0.05), and it was lower in the treatment group than in the control group after treatment (P<0.05). ④Medication compliance improved significantly in both groups after treatment compared to that before treatment (P<0.05), and it was significantly better in the treatment group than in the control group after treatment (P<0.05). Conclusion:Acupuncture and moxibustion show satisfactory efficacy in the treatmentof constipation in patients with middle- and advanced-stage Parkinson’s disease. They can effectively relieve constipation and improve the patients’ daily activities and medication compliance.关键词：Parkinson’s disease;middle- and advanced-stage;constipation;acupuncture and moxibustion
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发布时间：2022-08-31 - 摘要：Objective:To observe the effect of Yigong Powder on repair of spleen tissue structure by inhibiting inflammatory reactions in mice with anemia of chronic disease(ACD). MethodsSixty 8-week-old male C57 BL/6 mice were randomly assigned into blank control group, model group and Yigong Powder group, 20 mice in each group. Except the blank control group, the model of mice with ACD was copied in the other groups (lipopolysaccharide and zymosan A were injected intraperitoneally at day 1 and day 7 respectively). At 5 days after injection of zymosan A, Decoction of Yigong Powder (15.413 g·kg-1·d-1) was administered by gavage to mice in the Yigong Powder group and 0.2 ml of ultra-pure water was given by gavage to the blank control and model groups. After 1 week and 2 weeks of treatment, whole blood was collected, white blood cell (WBC) count, neutrophil count and hemoglobin level were determined with an automated cell counter; the spleen tissue was isolated to observe the structure by HE staining; and mitochondria of spleen was observed under electron microscope. Results:Compared to the blank control group, WBC count and neutrophil count increased and the hemoglobin level decreased (P<0.01), the spleen enlarged significantly, the spleen index increased (P<0.01), and the normal structure of mitochondria disappeared in spleen tissue in the model group. After 1 week and 2 weeks of treatment, compared to the model group, WBC count and neutrophil count decreased significantly and the hemoglobin level increased significantly (P<0.01); the spleen shrank significantly (P<0.01), and the spleen index decreased (P<0.01) in the Yigong Powder group. The mitochondrial structure was observed to be close to normal under electron microscope, and the number of damaged mitochondria was reduced. Conclusion:Yigong Powder improves anemia in mice with ACD by inhibiting inflammatory reactions and reducing damage to the spleen tissue structure caused by inflammatory factors.关键词：anemia of chronic disease;Yigong Powder;inflammatory reaction;structure of spleen tissue;mice
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发布时间：2022-08-31 - 摘要：Objective:To study the effect of Wenshen Formula on the expression of group 2 innate lymphoid cells (ILC2)-related molecules in asthmatic mice. MethodsThirty BALB/c mice were randomly assigned into the normal control (NC) group, the IL-33 group, the Western medicine (PRE) group, the Wenshen Formula (WSF) group and the combination (CO) group, 6 mice in each group. Except the normal control group, rmIL-33 nasal drops (0.4 μg/40 μl) were given to the other 4 groups for 6 consecutive days and starting at day 7, the drug was administered once every other day for 1 week. Drug invention was given by gavage for 15 days to the PRE group (prednisone acetate 1.125 g/kg), WSF group (Wenshen Formula 9 g/kg) and CO group (Wenshen Formula + prednisone acetate) at 1 hour before nasal administration. Lung tissue was stained with hematoxylin-eosin (HE) to observe morphology of the tissue and infiltration of inflammatory cells. ELISA was used to detect the expression of IL-4, IL-5 and IL-13 in bronchoalveolar lavage fluid (BALF). Fluorescence quantitative PCR was used to detect the expression of RORα in lung tissue. Results:①Compared to the NC group, the mucosal folds of lung tissue were thicker and infiltration of inflammatory cells was more obvious in the IL-33 group. Infiltration of inflammatory cells was reduced significantly in the WSF, PRE and CO groups. ②Compared to the NC group, the expression levels of IL-4, IL-5 and IL-13 in BALF of mice increased significantly in the IL-33 group (P<0.001). The expression levels of IL-4, IL-5 and IL-13 in BALF were reduced in the WSF, PRE and CO groups, with significant differences compared to the IL-33 group (P<0.01). ③Compared to the NC group, the expression of ILC2 RORα mRNA in lung tissue was increased significantly in the IL-33 group (P<0.001). The expression of RORα mRNA in lung tissue was down- regulated in the WSF, PRE and CO groups, with statistically significant differences compared to the IL-33 group (P<0.05). Conclusion:Wenshen Formula reduces infiltration of the airway inflammatory cells and alleviates asthmatic inflammation in mice. It down-regulates the expression of IL-4, IL-5 and IL-13 and of RORα mRNA.关键词：Wenshen Formula;asthma;ILC2;mice
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发布时间：2022-08-31 - 摘要：Objective:To explore the mechanism of action of Feiyanning Recipe (FYN) in inhibiting invasion of the A549 lung cancer cells by regulating metastasis-associated lung adenocarcinoma transcript 1 (MALAT1). MethodsThe A549 cells transfected with LV-vector and LV-over/MALAT1 were treated with FYN (0～500 μg/ml). The expression of MALAT1 was detected by qRT-PCR; the effect of FYN on tumor cell proliferation was determined by MTT colorimetric assay; the invasive ability of tumor was determined by cell invasion test; and the expression of Wnt/β-catenin/EMT signaling pathway related proteins in A549 cells was detected by Western blotting. Results:Results of the cell invasion test indicated that MALAT1 overexpression strengthened the invasive ability of the A549 cells, and there was significant difference compared to the normal control group and the lentivirus vector group (P<0.01). Compared to the normal control group, FYN inhibited invasion of the A549 cells, with significant difference (P<0.01), but the ability of FYN to inhibit cell invasion was reduced after MALAT1 overexpression. Results: of MTT colorimetric assay showed that FYN inhibited proliferation of the A549 cells in a concentration-dependent manner. Results: of qRT-PCR indicated that FYN down-regulated the expression of MALAT1. Results: of Western blotting showed that MALAT1 down-regulated E-cadherin and up-regulated N-cadherin, Snail and β-catenin in the nucleus, whereas FYN up-regulated E-cadherin and down-regulated N-cadherin, Snail and β-catenin in the nucleus, but MALAT1 overexpression reduced the regulatory effect of FYN on these proteins. Conclusion:MALAT1 overexpression promotes lung cancer cell invasion by activating the Wnt/β-catenin pathway and accelerating the EMT progress. Feiyanning Recipe inhibits invasion of the A549 lung cancer cells by down-regulating MALAT1 and suppressing the Wnt/β-catenin/EMT signal axis.关键词：lung cancer;Feiyanning Recipe;A549 lung cancer cells;MALAT1;EMT;Wnt/β-catenin pathway
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发布时间：2022-08-31 - 摘要：Objective:To observe the changes of gastrointestinal motility in rats with functional dyspepsia (FD), investigate the difference in the effect of regulating gastrointestinal motility following different acupoint pressure sequences and explore the sequence effect of “first opening Middle Jiao” in zang-fu viscera acupoint pressure therapy. Methods60 SD rats were randomly assigned into the blank group, the model group, the Upper-Middle Jiao group, the Middle-Upper Jiao group and the Middle-Lower Jiao group, 12 rats in each group. Except the blank group, rats in the other groups were treated with mild tail-clamping stimulation plus intermittent diet for 21 days for modeling. Each group was given corresponding intervention for 14 consecutive days after successful modeling. The behavior, electrogastrogram, intragastric residual rate and small intestinal propulsion rate of rats were observed and the expression of c-kit was detected in the gastric antrum tissue in each group. Results:The gastric electrical amplitude was lower in the model group than in the blank group (P<0.05); it was higher in the Upper-Middle Jiao and Middle-Lower Jiao groups than that in the model group (P<0.05); the gastric residual rate was higher and the small intestinal propulsion rate was lower in the model group than in the blank group (P<0.05); the gastric residual rate was lower and the small intestinal propulsion rate was higher in the Middle-Lower Jiao group than in the model group (P<0.05); and in the manipulation intervention groups, the small intestinal propulsion rate was higher in the Middle-Lower Jiao group than in the Upper-Middle Jiao and Middle-Upper Jiao groups (P<0.05). The mean optical density (OD) value of c-kit in the gastric antrum tissue was lower in the model group than in the blank group (P<0.01), with decrease in c-kit positive interstitial cells of Cajal (ICCs) and a lighter color. The mean OD value was higher in the Middle-Lower Jiao group than in the model group (P<0.05), with increase in c-kit positive ICCs and a darker color. Conclusion:Zang-fu viscera acupoint pressure therapy can effectively treat gastrointestinal motility disorder in FD rats, upregulating the expresion of c-kit and “first opening Middle Jiao” in the Middle-Lower Jiao group shows the best effect.关键词：gastrointestinal motility disorder;zang-fu viscera acupoint pressure therapy;electrogastrogram;gastric residual rate;small intestinal propulsion rate;c-kit;rats
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发布时间：2022-08-31 - 摘要：Objective:To study the effects of Ganmai Dazao Decoction on the expression of brain-derived neurotrophic factor (BDNF) and serotonin transporter (SERT) genes in prefrontal lobe and amygdala and on the serum level of 5-hydroxytryptamine (5-HT) in depression model rats. Methods40 male Wistar rats were randomly assigned into normal group, model group, Ganmai Dazao Decoction group and fluoxetine hydrochloride group, 10 rats in each group. Except the normal group, the depression model was established by chronic unpredictable mild stress (CUMS) combined with orphaned method in the other groups. After modeling, Ganmai Dazao Decoction concentrate solution (12.5 ml/kg) was given to the Ganmai Dazao Decoction group, fluoxetine hydrochloride solution (10 ml/kg) was administered to the fluoxetine hydrochloride group and 2 ml of distilled water was given to the normal and model groups. All groups were treated for 28 days. The serum level of 5-HT in rats was detected by GC-MS; and the relative expression of BDNF mRNA and SERT mRNA in prefrontal lobe and amygdala was determined by real-time quantitative PCR. Results:①The serum level of 5-HT and the relative expression of BDNF mRNA and SERT mRNA in prefrontal lobe and amygdala decreased in the model group compared to the normal group (P<0.05). ②Compared to the model group, the serum level of 5-HT increased in the Ganmai Dazao Decoction and fluoxetine hydrochloride groups (P<0.05); the relative expression of BDNF mRNA in prefrontal lobe increased in the Ganmai Dazao Decoction group (P<0.05) and the relative expression of BDNF mRNA and SERT mRNA in prefrontal lobe increased in the fluoxetine hydrochloride group (P<0.05); and the relative expression of BDNF mRNA and SERT mRNA in amygdala increased in the Ganmai Dazao Decoction and fluoxetine hydrochloride groups (P<0.05). ③There were no statistically significant differences in the serum level of 5-HT and the relative expression of BDNF mRNA and SERT mRNA in prefrontal lobe and amygdala between the Ganmai Dazao Decoction group and the fluoxetine hydrochloride group (P>0.05). Conclusion:Ganmai Dazao Decoction may treat depression by increasing the serum level of 5-HT and the expression of BDNF mRNA in prefrontal lobe and amygdala and SERT mRNA in amygdala.关键词：depression;rats;Ganmai Dazao Decoction;5-HT;BDNF;SERT;gene expression
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- 562
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发布时间：2022-08-31 - 摘要：Objective:To investigate the effects of Yinqi Tiaozhi Decoction on lipid metabolism in rats with nonalcoholic fatty liver disease (NAFLD) and the mechanism of action. Methods48 SD rats were randomly assigned into normal group, model group, simvastatin (2.08 mg/kg) group, low-dose (8.28 g/kg), middle-dose (16.56 g/kg) and high-dose (33.12 g/kg) Yinqi Tiaozhi Decoction groups. Except the normal group, high-fat diet was given to rats in the other 5 groups for 12 weeks to establish the NAFLD rat model. After successful modeling, corresponding drugs were administered by gavage to the drug groups, and 0.9% NaCl solution of equal volume was given by gavage to the normal and model groups. At 8 weeks of treatment, wet weight of the liver and liver index were calculated, the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), cholesterol (TC) and triglyceride (TG) in serum were detected; the levels of TC, TG, malondialdehyde (MDA), superoxide dismutase (SOD) and free fatty acid (FFA) in liver tissue were determined by ELISA; hematoxylin-eosin (HE) staining and oil red O staining were performed to observe the pathology of liver tissue; and the relative expression of liver X receptor α (LXRα) and sterol regulatory element-binding protein-1c (SREBP-1c) in liver tissue was detected by polymerase chain reaction (PCR). Results:①Compared to the normal group, the body mass, wet weight of the liver and liver index increased (P<0.05); the levels of ALT, AST, TC and TG in serum increased (P<0.05), the levels of TC, TG, MDA and FFA, the relative expression of LXRα and SREBP-1c mRNA in liver tissue increased (P<0.05) and the SOD level decreased (P<0.05) in the model group. ②Compared to the model group, the body mass, wet weight of the liver and liver index decreased (P<0.05); the levels of ALT, AST, TC and TG in serum decreased (P<0.05), the levels of TC, TG, MDA and FFA and the relative expression of LXRα and SREBP-1c mRNA in liver tissue decreased (P<0.05) and the SOD level increased (P<0.05) in the simvastatin group and Yinqi Tiaozhi Decoction groups. ③Compared to the simvastatin group, the levels of ALT and AST in serum decreased in the middle-dose and high-dose Yinqi Tiaozhi Decoction groups (P<0.05), the body mass, wet weight of the liver and liver index decreased, the levels of FFA and MDA and the relative expression of LXRα mRNA and SREBP-1c mRNA decreased (P<0.05), and the SOD level increased (P<0.05) in the high-dose Yinqi Tiaozhi Decoction group. ④The body mass, wet weight of the liver and liver index decreased significantly in the high-dose group compared to the low-dose group (P<0.05); the levels of ALT and AST in serum decreased in the middle-dose group compared to the low-dose group (P<0.05), and the levels of ALT, AST, TC and TG decreased in the high-dose group compared to the low-dose group (P<0.05). The levels of TC and TG (middle-dose group vs. low-dose group) and of FFA, MDA and SOD (high-dose group vs. low- and middle-dose groups) decreased significantly with increase in dose; and the relative expression of LXRα mRNA and SREBP-1c mRNA in liver tissue decreased in the high-dose group compared to the low-dose group (P<0.05). Conclusion:Yinqi Tiaozhi Decoction reduces significantly the levels of blood and liver lipid in in rats with NAFLD, inhibits lipid accumulation, reduces oxidative stress, protects liver function, and improves pathological injury of the liver. The mechanism of action may be related with inhibition of the expression of LXRα and SREBP-1c.关键词：non-alcoholic fatty liver disease;rats;Yinqi Tiaozhi Decoction;lipid metabolism;LXRα;SREBP-1c
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发布时间：2022-08-31 - 摘要：Objective:To establish the method of quantitative analysis of multi-components by single-marker (QAMS) in the determination of five flavonoids in the flower of Salix caprea L. MethodsUsing rutin as the reference substance, the relative correction factors of luteolin-7-O-β-D-pyranoside, apigenin-7-O-β-D-pyranoside, luteolin and apigenin were calculated by high performance liquid chromatography (HPLC). Related influencing factors were investigated and the relative correction factors were determined. The content of the other four components was calculated directly using the content of rutin and the relative correction factors to establish the QAMS method with rutin as the reference substance. The results were compared with those obtained by external standard method. Results:The relative correction factors were fluteolin-7-O-β-D- pyranoside / rutin =1.49 (RSD=1.84%), fapigenin-7-O-β-glucopyranoside / rutin =2.26 (RSD=2.26%), fluteolin / rutin =2.63 (RSD=1.01%) and fapigenin / rutin =2.86 (RSD=1.55%), with good repeatability. There were no significant differences between the values obtained with the QAMS method and those obtained with external standard method, and the correction factor obtained was reliable. Conclusion:It is feasible and accurate to determine the content of five flavonoids in flower of Salix caprea L. by the QAMS method, which can be used for quality evaluation of the flower.关键词：flower of Salix caprea L.;flavonoid components;QAMS;HPLC;relative correction factor
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发布时间：2022-08-31 - 摘要：This paper reviews the relationship between macrophage polarization and atherosclerosis and the intervention effects of traditional Chinese medicine. M1 type macrophage polarization can promote the development of atherosclerosis, while M2 type macrophage polarization plays a dual role on atherosclerosis. The extracts of traditional Chinese medicine such as Danshen(Radix SalviaeMiltiorrhizae), Chuɑnxinlian(HerbaAnorographitis), Jianghuang(RhizomaCurcumaeLongae), Guipi(Cortex CinnamomiJaponici), Huɑnglian(RhizomaCoptidis)can control the progression of atherosclerosis by regulating the polarization of macrophages.关键词：macrophage;polarization;atherosclersis;traditional Chinese medicine;review
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发布时间：2022-08-31 - 摘要：This paper reviews the research progress in chemical constituents and pharmacological effects of Pulsatilla Decoction. The chemical components of Pulsatilla Decoction mainly include alkaloids,coumarins,saponinsand limonoids. Modern pharmacological research shows that Pulsatilla Decoction has pharmacological effects such as anti-inflammatory,antibacterial,immune regulation and anti-tumor.关键词：Pulsatilla Decoction;chemical composition;pharmacological action;review
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发布时间：2022-08-31
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