摘要:This paper introduces Professor Lu Jin’gen’s clinical experience in the treatment of outlet obstructive constipation exemplified by the constipation cases due to anismus, pelvic floor relaxation (PFR), puborectal muscle hypertrophy (PRMH) and rectocele. In the clinic, Professor Lu does not advocate the over-use of purgative medicinals such as Dahuang (Rhei Radix et Rhizoma), Luhui (Alo) and Fanxieye (Sennae Folium), etc. as he believes that this type of constipation should be diagnosed with the combination of disease differentiation and TCM pattern identification and he proposes that invigorating qi, relieving constipation and unblocking collaterals should be taken as the major method. A proved case is attached for the supportive illustration.  
摘要:This paper introduces Professor Yang Jinkun’s clinical experience in the treatment of primary liver cancer with therapy of invigorating spleen for removing phlegm. He considers that spleen deficiency and phlegm accumulation is the main pathogenesis of primary liver cancer. The treatment should be based on invigorating spleen and replenishing qi, combined with resolving phlegm. According to the syndrome differentiation, different therapies are selected, such as therapy of eliminating dampness and resolving phlegm, therapy of softening hardness and resolving phlegm, therapy of warming yang to dissipate phlegm, therapy of regulating qi-flowing for eliminating phlegm. These therapies show good efficacy. One proven case is presented.  
关键词:primary liver cancer;spleen deficiency and phlegm accumulation;invigorating spleen and resolving phlegm;experience of famous doctor;Yang Jinkun
摘要:This paper introduces Professor Zhan Hongsheng’s clinical experience in treating osteoporosis. The etiology and pathology of osteoporosis is closely related to the liver, spleen and kidney, and to tendon, muscle and skeleton. The treatment should focus on reducing the risk of fracture, emphasizing the restoration of muscle strength and baby balance, treating the spleen and kidney simultaneously and the muscle and skeleton together. And the concept of “harmony in four aspects” runs through the whole treatment. One proven case is presented.  
关键词:osteoporosis;therapy of traditional Chinese medicine;experience of famous doctor;Zhan Hongsheng
摘要:The clinical experience of disease-pattern combination mode in the treatment of hyperuricemia is introduced. It is believed that the diagnosis and treatment mode of disease-pattern combination is a breakthrough and development of the theory of combination of disease differentiation and pattern identification. In the clinic, it is supposed to differentiate the disease first, identify the pattern later within a specific disease framework, and then define the mechanism and finally choose appropriate medicines for treatment. Two proved cases are attached.  
摘要:Taking the physiological function of the kidney as the starting point, combining with the theoretical origin and clinical application, this paper expounds on the correlation between “kidney governing the bone and generating marrow” and cervical spondylotic myelopathy (CSM), and holds that “tonifying the kidney and supplementing the marrow” is an important principle of traditional Chinese medicine in the treatment of CSM. A clinically proved case is attached as supportive evidence in the hope of providing diversified therapeutic approaches for the treatment of CSM.  
关键词:cervical spondylotic myelopathy (CSM);kidney-tonifying and marrow-supplementing method;Chinese medicine theory;acupuncture and moxibustion;tuina massage;Chinese medicine
摘要:Professor Wu Yin’gen believes that kidney yang deficiency pattern, as the basic core pattern of asthma, has a significant influence on the occurrence, development and prognosis of asthma. In the treatment of asthma, he suggests taking warming and tonifying the kidney yang as the basic treatment method and has developed a new formula Zhichuan (cough-relieving) capsule. Meanwhile, considerations and prospects are put forward in this paper, including the comprehensive analysis of the influence of genetic and environmental factors on the kidney yang deficiency pattern of asthma, as well as the discussion of the molecular mechanism and clinical research on the treatment of asthma by warming and tonifying the kidney yang method.  
关键词:bronchial asthma;kidney yang deficiency pattern;warming and tonifying kidney yang method;Wu Yin’gen
摘要:This paper discusses the clinical experience of using Xiaoxuming decoction in the treatment of stroke under the guidance of the theory of “exogenous wind”. It is considered that “exogenous wind” is the main aspect of the pathogenesis of stroke, and the prescription of treating wind plays a dominant role in the treatment. By summing up the clinical and experimental research results, this paper expounds the mechanism of Xiaoxuming decoction in the treatment of stroke.  
摘要:Objective:To study the diversity of gut microbiome in pancreatic cancer patients with damp-heat syndrome based on high-throughput sequencing technology. This study aims to exploring the role of gut microbiome in the pathogenesis of pancreatic cancer,and providing new ideas for clinical diagnosis and treatment. MethodsThirty-one cases of pancreatic cancer patients with damp-heat syndrome who were treated in our hospital from January 2018 to December 2018 were served as observation group. Another 8 cases of normal people who received a physical examination at the same time were served as control group. Stool samples were collected from two groups of people. DNAs were extracted and the database was constructed. The high-throughput sequencing was performed on the Illumina Hiseq PE150 platform,and then the biological information of the samples was obtained. The species annotation and functional annotation of the samples between the two groups were analyzed and compared. Results:① After deredundancy, 328 787 mitochondrial open reading frames (ORFs) were obtained. The total number of genes in the two groups was 137 849, the unique gene of the observation group was 114 439, and the unique gene in the control group was 60 473.② There was a significant difference in the species abundance between the two groups at the level of the door and the level of the genus. On the level of the door, the abundance of the flora in the observation group was observed, such as the abundance of Proteobacteria, Acinetobacteria, Verrucomicrobia, Synergistertes and Fibrobacteres, which was higher than that of the control group. But the abundance of Bacteroidetes, Firmicutes, Spirochaetes, Candidatus and Chlamydiae was lower than that of the control group. At the genus level, the abundance of Akkermansia, Collinsella, Streptococcus, Bilophila was higher than that of the control group, while the abundance of Bifidobacterium, Prevotella, Bacteroides, Faecalibacterium, Clostridium, Roseburia was lower than that of the control group.③ The principal coordinate analysis (PCoA) based on distance matrix showed that there was significant difference in the composition and structure of flora between the two groups.④ The results of functional interpretation and analysis of KEGG database showed that the gene expression level in metabolism, environmental information processing and cell processing related pathways in the observation group was higher than that in the control group, and the distribution of functional abundance between the observation group and the control group could be distinguished obviously. Conclusion:Compared with normal people,the intestinal flora of pancreatic cancer patients with damp-heat syndrome has obvious differences in diversity,flora structure and function,so monitoring the changes of intestinal flora can provide valuable information for clinical prevention and treatment of pancreatic cancer.  
关键词:high-throughput sequencing technology;pancreatic cancer;damp-heat syndrome;gut microbiome;composition and structure;diversity;gene function
摘要:To discuss the treatment experience of acne by Chaihu Guizhi Ganjiang decoction combined with Tuo(pus-expelling) method in TCM surgery. It is suggested that the combination of Chaihu Guizhi Ganjiang Decoction and Tuo (pus-expelling) method is mainly used to treat acne of cold and heat complex pattern and upper-heat and lower-cold pattern. One proved case is exemplified.  
摘要:The harmonizing method in the six channels and nine differentiation methods is divided into the methods of regulating Ying and Wei, adjusting rise and fall, considering cold and warm. The harmonizing method is expounded respectively from aspects of the main points of diagnosis and treatment, treatment method and prescription and its evolution in later generations. The corresponding basic prescriptions, analogical prescriptions and modified prescriptions are summarized.  
关键词:six channels and nine differentiation methods;method of regulating Ying and Wei in Taiyang channel;method of adjusting rise and fall in Shaoyang channel;method of considering cool and warm in Jueyin channel;main points of diagnosis and treatment;modified prescriptions
摘要:This paper introduces Wang Mengying’s academic thoughts on preserving body-fluid and nourishing yin, mainly summarizing the clinical application characteristics of Wang’s preserving body-fluid and nourishing yin from four aspects, such as removing pathogenic factors and preserving body-fluid and nourishing yin, especially focusing on the lung and stomach; nourishing yin in the lung and stomach being different from the kidney; a large number of sweet-cold Chinese medicine nourishing and saving critical patients; combination of medicine and food having great obvious effect.  
关键词:Wang’s Medical Case;Wang Mengying;preserving body-fluid and nourishing yin
摘要:Objective:To observe the clinical effect of Yishen Xiezhuo (kidney-supplementing, turbidity-discharging and stasis-dissolving) decoction on diabetic kidney disease (DKD). MethodsRandomly divide 68 cases of DKD into the control group and the treatment group with 34 cases each. The control group was treated with routine medication plus valsartan, and the treatment group was given Yishen Xiezhuo (kidney-supplementing, turbidity-discharging and stasis-dissolving) decoction along with the treatment in the control group. The course of treatment in both groups was 12 weeks. The clinical effect, TCM pattern score, as well as the level changes of serum creatinine (Scr), estimated glomerular filtration rate (eGFR), microalbuminuria (MA), fibrinogen (Fbg) and D-Dimer (D-Di) were observed. Results:①There were 60 cases in the final statistical analysis including 31 cases in the control group and 29 cases in the treatment group as 8 cases were excluded during the clinical observation. ②The clinical effective rate of the treatment group and the control group were 86.2% and 71.0% respectively; the clinical effect of the treatment group was better than that of the control group (P<0.05). ③TCM pattern scores in the two groups both decreased significantly after treatment (P<0.05); TCM pattern scores in the treatment group were lower than those in the control group (P<0.05). ④The levels of Scr and MA in the two groups decreased while the levels of eGFR increased after treatment (P<0.05); and the improvement in the treatment group was more significant compared to that of the control group according to the levels of Scr, eGFR and MA after treatment (P<0.05). ⑤The levels of Fbg and D-Di in the treatment group decreased significantly after treatment (P<0.05), but there was no obvious change of the levels of Fbg and D-Di in the control group (P>0.05); The levels of Fbg and D-Di in the treatment group was lower than those in the control group (P<0.05). Conclusion:The clinical effect of Yishen Xiezhuo (kidney-supplementing, turbidity-discharging and stasis-dissolving) decoction combined with valsartan is better than that of valsartan alone in the treatment of DKD as together they can effectively reduce the urinary protein, control the coagulation disorder and improve the renal microcirculation and renal function, as well as alleviate clinical symptoms for patients with DKD.  
摘要:Objective:To observe the clinical efficacy of Xiadan ointment moving cupping therapy in the treatment of stable plaque psoriasis. MethodsSeventy-two patients with stable plaque psoriasis were randomly assigned into treatment group (37 patients) and control group (35 patients). The treatment group was treated with Xiadan ointment moving cupping therapy, and the control group with paraffin oil moving cupping therapy. The treatment course was 1 month in both groups. The clinical efficacy was observed, the changes in psoriasis area severity index (PASI) score were compared and safety was evaluated in the groups. Results:①The total effective rate was 71.6% and 52.8% in the treatment group and the control group, respectively; the clinical efficacy was superior in the treatment group to that in the control group (P<0.05). ②The PASI score decreased significantly in the groups after treatment (P<0.05); there were greater differences before and after treatment in the treatment group than in the control group, with significant differences (P<0.05). ③There were no severe adverse reactions in the groups. Conclusion:The Xiadan ointment moving cupping therapy is effective in the treatment of stable plaque psoriasis and leads to no remarkable adverse reactions, worthy of further extension and application in clinical practice.  
摘要:Objective:To observe the clinical effect of TCM three-dimensional traction in the treatment of cervical spondylotic radiculopathy (CSR). MethodsEighty cases of CSR were randomized into the treatment group and the control group with 40 cases in each group. The treatment group was treated with TCM three-dimensional traction device (three-dimensional intermittent traction), while the control group was treated with traditional cervical traction chair (traditional continuous traction). The course of treatment in both groups was 2 weeks. The safety of treatment was observed and the changes of Visual Analogue Scale (VAS) and Neck Disability Index (NDI) were compared. Results:①There was no drop-out case during the trial. ②The scores of VAS and NDI of the two groups before and after treatment respectively were significantly reduced (P<0.05); After treatment, the VAS score and NDI score of the treatment group were significantly lower than those of the control group (P<0.05). ③The incidence of adverse reactions in the treatment group was significantly lower than that in the control group (P<0.05). Conclusion:Compared with the traditional cervical traction, TCM three-dimensional traction can better relieve the pain and dysfunction with fewer adverse reactions in the treatment of CSR.  
摘要:Objective:To explore the therapeutic efficacy of Bushen Huayu decoction coloclysis in the treatment of ovarian endometriosis(EMT). MethodsSixty-four patients with ovarian EMT were included and randomly divided into the treatment group (33 cases) and the control group (31 cases). The patients in the treatment group were treated with Bushen Huayu decoction coloclysis and the patients in the control group were orally treated with Guizhi Fuling decoction, with a course of 3 months. The scores of dysmenorrhea and the cyst volume of endometriosis in ovary between the two groups were compared; the levels of the liver and kidney function indexes were detected and the adverse reactions were recorded during the course of treatment in order to evaluate the safety of treatment. Results:After treatment, the cyst volumes in ovary in both groups were significantly reduced compared with treatment before (P<0.05), and the reduction degree of the cyst volume in ovary in the treatment group was more obvious compared with the control group (P<0.05); the scores of dysmenorrhea in both groups were significantly decreased compared with treatment before (P<0.05), and the reduction degree of the dysmenorrhea scores in the treatment group was more obvious compared with the control group (P<0.05). No serious adverse reactions and abnormity of liver and kidney function happened in the course of treatment. Conclusion:Bushen Huayu decoction coloclysis can effectively improve the dysmenorrhea symptom and reduce the cyst volume in ovary in patients with ovarian EMT, which has better safety.  
关键词:Bushen Huayu decoction;coloclysis;ovarian endometriosis;traditional Chinese medical therapy
摘要:Objective:To observe the clinical effect of Kangmin Zhike (anti-allergy and cough-relieving) decoction combined with Kechuanning (cough-asthma-calming) acupoint application on children with cough variant asthma (CVA) due to wind-cold. MethodsRandomly divide 90 children with CVA into the control group, the decoction group and the combination group with 30 cases each. The control group was given montelukast sodium chewable tablets, the decoction group was given Kangmin Zhike (anti-allergy and cough-relieving) decoction orally, and the combination group was given Kangmin Zhike (anti-allergy and cough-relieving) decoction and Kechuanning (cough-asthma-calming) acupoint application. All the three groups were treated for 8 weeks. The scores of TCM patterns, the changes of percentage of forced expiratory volume in 1 second (FEV1%), percentage of predicted peak expiratory flow (PEF%) and fractional exhaled nitric oxide (FeNO) concentration were observed and compared, and the recurrence was also observed 2 months after the treatment. Results:①The differences of TCM pattern scores in each group before and after treatment were significant (P<0.01); according to the comparison of difference values calculated based on TCM pattern scores before and after treatment, the decoction group showed better improvement than the control group (P<0.05) and the improvement in the combination group was even more significant than that in the control group (P<0.01) and that in the control group (P<0.01) and that in the decoction group (P<0.05). ②The FEV1% and PEF% of the three groups all increased after treatment (P<0.05), and the increase of FEV1% and PEF% was especially significant in the combination group (P<0.01); Based on the comparison of FEV1% and PEF% among three groups after treatment, the improvement in the decoction group was better than that in the control group (P<0.05) and the improvement in the combination group was better than that in the control group (P<0.01) and that in the decoction group (P<0.05). ③The levels of FeNO of the three groups all decreased when compared before and after treatment (P<0.05), and the decrease of FeNO level was more significant in the combination group (P<0.01); According to the comparison of FeNO levels among groups after treatment, the decoction group had a lower FeNO level than the control group (P<0.05), and the combination group had an even lower FeNO level than the control group (P<0.01) and the decoction group (P<0.05). ④The recurrence rates of the control group, decoction group and combination group were 32.00%, 18.52% and 7.14% respectively, and the differences were statistically significant (P<0.01). ⑤During the trial, no serious adverse reactions were found in each group, and no abnormality was found in blood routine test, urination and stool routine test, and liver and kidney function test. Conclusion:The combination of Kangmin Zhike (anti-allergy and cough-relieving) decoction and Kechuanning (cough-asthma-calming) acupoint application can significantly improve the symptoms, pulmonary function and airway inflammation of children with wind-cold type CVA, and reduce the recurrence rate.  
摘要:Objective:To explore the effects of Huanglian(Rhizoma Coptidis)-Dahuang(Radix et Rhizoma Rhei)-Rougui(Cortex Cinnamomi)compound and its best components compatibility on tumor proliferation and syndromes of liver cancer mice. MethodsHuman hepatocarcinoma cells were injected subcutaneously into the right axilla to establish a liver cancer model of transplanted nude mice.After modeling success,mice were divided into model control group(model group),Huanglian(Rhizoma Coptidis)-Dahuang(Radix et Rhizoma Rhei)-Rougui(Cortex Cinnamomi)compound group(compound group) and berberine-emodin-cinnamaldehyde group (compatibility group). Each group was treated with corresponding drug for 2 weeks.During the administration, the state of the mice was observed.Before and after administration,the tumor diameter and volume,body weight, axillary temperature, grip force, number of horizontal movements and vertical standings in the squat field, microphotograph of the claws and tails were measured twice.The degree of excess or deficiency of Qi,Xue,Yin and Yang were calculated according to the above indexes.The ratios of post-treatment/pre-treatment of these indexes were used to analyze the change of syndromes.Finally, the mice were sacrificed and the tumors were weighed and the tumor inhibition rate was measured. Results:①Mice state:During the experiment, the mice were all in good condition, both the movement and the defecation were normal.②Antitumor effect:The tumor volume of each group increased with time,which was displayed as the model group> compound group>compatibility group. The variation of tumor weight was consistent with that of the tumor volume.③Syndrome change:After treatment,the degree of excess or deficiency of Qi was increased,and the degree of the compatibility group was better than that of the compound group.The degree of excess or deficiency of Xue was also raised,and the degree of the compound group was superior to the compatibility group.The degree of excess or deficiency of Yin and Yang almost had no change. Conclusion:Both the compound and the best compatibility of the medicines can inhibit the tumor proliferation of liver cancer mice and has the trend of invigorating qi and blood.  
关键词:Huanglian(Rhizoma Coptidis)-Dahuang(Radix et Rhizoma Rhei)-Rougui(Cortex Cinnamomi)compound;components compatibility;liver cancer;mice;syndrome
摘要:Objective:ObjectiveTo investigate the effects of Qinggan Yishen Qufeng formula (QYQ) on the dual pathological conditions of myocardial injury and high blood pressure as well as the possible mechanism. MethodsSpontaneously hypertensive rats(SHRs)were randomly divided into QYQ-treated group(QYQ),Candesartan-treated group(Can)as well as the vehicle-treated groups(SHR). Eight male WKY rats of the same age were used as normal control group (WKY).All experimental animals were subject to drug intervention at 6 weeks of age for 14 weeks. The systolic and diastolic blood pressure levels were measured by the tail pressure method at every 2 weeks in each group to evaluate the effect of QYQ. At the age of 20 weeks, the cardiac tissue was taken and paraffin embedded section was performed. HE staining was used to calculate the cross-sectional area of the cardiac muscle cells to evaluate the preventive effect of QYQ on the hypertension-associated myocardial injury. The differential expression of miR-125a-5p and its target genes, such as IL-6R, STAT3 and IL-6 mRNA in cardiac tissue was further tested. Results:Compared with the model group, QYQ significantly decreased the systolic blood pressure (SBP) and diastolic blood pressure (DBP) of SHR rats, decreased the cross section area of cardiomyocytes (P<0.05), upregulated the expression of miR-125a-5p in cardiac tissue, down-regulated the expression of direct target gene, such as IL-6R,STAT3 mRNA, and down-regulated the expression of IL-6 mRNA in cardiac tissue (P<0.05). Conclusion:QYQ not only has significant antihypertensive effect, but also has significant preventive effect on hypertension-associated myocardial injury. The regulatory effect of QYQ on miR-125a-5p/IL-6R/STAT3 pathway may be one of the potential molecular mechanisms of QYQ in the prevention and treatment of hypertension-associated myocardial injury.  
摘要:Objective:To investigate the effect of Yiqifusheng prescription on the proliferation and migration of human gastric cancer cell line BGC-823. MethodsMTT assay was used to detect the inhibitory effect of Yiqifusheng prescription on BGC-823 cell viability. The effect of Yiqifusheng prescription on cell migration was tested by wound healing test in BGC-823 cell. Flow cytometry was used to determine the effects of Yiqifusheng prescription on cell cycle and apoptosis. Results:Compared to the blank control group, BGC-823 cells treated with various concentrations of Yiqifusheng prescription (0.05 mg/mL,0.1 mg/mL,0.2 mg/mL,0.4 mg/mL,0.8 mg/mL,1 mg/mL) decreased cell activity. The wound healing test results showed that the BGC-823 cell migration ability of Yiqifusheng prescription-treated group was reduced than that of the blank control group. Cell cycle test showed that Yiqifusheng prescription could block the cycle progression of human gastric cancer cells BGC-823.Apoptosis test showed that Yiqifusheng prescription could promote cell apoptosis of human gastric cancer cells. Conclusion:Yiqifusheng prescription can significantly inhibit the proliferation and migration of human gastric cancer cell,and play an anti-growth role in gastric cancer cells of BGC-823.  
摘要:Objective:To optimize the processing technology of charring chrysanthemum. MethodsCharred Chrysanthemum samples were prepared by means of L9 (34) orthogonal test design, and the contents of chlorogenic acid, luteolin, 3,5-O-di-coffee acyl quinic acid were determined by high performance liquid chromatography(HPLC). The gradient elution was carried out with acetonitrile as mobile phase A and 0.1% aqueous phosphoric acid as mobile phase B. The flow rate was 1.0 mL/ min, the detection wavelength was 348 nm, and the column temperature was 30 ℃. And the appearance character identification, the water content and the extract content determination were carried out. The optimum processing technology and the specific process parameters of charring chrysanthemum were selected based on the appearance character, the extract content and the content of the chlorogenic acid, the luteolin, the 3,5-O-di-coffee and the quinine acid. ResultThe optimum charring process of the chrysanthemum was as follows:1 000 g of chrysanthemum put in a hot pot with a temperature of 300 ℃, fried for 6 minutes. Conclusion:The optimized technology is stable,feasible and the technical paramater is definite.  
摘要:This paper reviews the mechanisms and progress of Chinese medicine against Candida. Studies have shown that some monomer components,extracts,and prescriptions of traditional Chinese medicine can inhibit the growth of pathogenic Candida. Besides restraining the expression of virulence factors(hydrolase,mycelium,biofilm)and related genes,they can promote the production of endogenous reactive oxygen species and aggravate the oxidative damage of yeast cells.  
关键词:Candida;traditional Chinese medicine;monomer;mechanism research;review
摘要:The relevant factors in sleep-awakening mechanism and research progress in its regulation with Chinese medicine are reviewed. With the development of neuroanatomy, pharmacology and molecular biology, it has been found that sleep-awakening mechanism is related to many factors, such as the thalamus-pituitary-adrenal axis, the melatonin system, dopamine, 5-hydroxytryptamine, neuropeptide, acetylcholine and gamma-aminobutyric acid etc. Recipes in TCM like Modified Sini Powder and Modified Xiaoyao Powder can influence the sleep-awakening mechanism by regulating hormone levels and neurotransmitters.  
关键词:sleep-awakening rhythm;therapy in traditional Chinese medicine;molecular neurobiology;regulatory mechanisms;review