最新刊期
期 6 ， 2018
- 摘要：This paper reviews the advances in pharmacological effects of Hemerocallis fulva. In clinic,it is often used in the treatment of hepatitis and insomnia. Pharmacological studies have found that it shows various effects,such as sedation and tranquillization,anti-depression,liver protection,antitumor,antioxidation,lipid lowering and so on.关键词：Hemerocallis fulva;pharmacological effects;insomnia;depression;anti-oxidation;review
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发布时间：2022-08-31 - 摘要：This paper summarizes Professor Shen Shuwen’s clinical experience in treating precancerous lesions of gastric cancer. It is considered that this disease often presents the syndrome of intermingled deficiency and excess. The deficiency mainly manifests deficiency of both qi and yin,and the excess is actually qi stagnation,dampness obstruction,phlegm stagnation and toxin accumulation based on deficiency,and the core is intermingled toxin and blood stasis. The therapy should benefit qi and nourish yin,remove toxic substance and resolve blood stasis. It emphasizes invigorating spleen and stomach and nourishing source of qi and blood,restoring qi and yin and taking reinforcement and elimination in combination,removing toxin and blood stasis obstruction. One proven case is presented.关键词：precancerous lesions of gastric cancer;therapy of traditional Chinese medicine;experience of famous doctor;Shen Shuwen
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发布时间：2022-08-31 - 摘要：Drug-induced syndrome is a new academic problem in the clinical practice of traditional Chinese medicine. It origins from the change of body microenvironment caused by drug adverse reactions,and affects the original syndrome. It belongs to the secondary new disease caused by drug in the treatment of certain disease. Combined with the clinical common drug-induced syndromes,it is considered that the clinical epidemiological investigation of drug-induced syndromes should be carried out extensively,and the standardized animal model of drug-induced syndromes should be developed and the material basis of its occurrence should be explored,which could help to improve the overall efficacy of integrated traditional Chinese and western medicine.关键词：drug-induced syndromes;Chinese medicine;animal model;material basis
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发布时间：2022-08-31 - 摘要：Internal injury caused by excess of seven emotions can lead to seminal deficiency and male infertility. Such as jealousy and narrow-mind,or sorrow and worry,or thoughtful and overtaxed mind-spirit,or irritability and indulgence,or stagnation of liver qi,or even the discord of couples feelings,all the ones can lead to infertility. These can be named as infertility due to depression syndrome. The main pathogenesis is disharmony between heart and kidney that impair spirit,liver stagnation transforming into fire that impair yin,spleen failing to govern transportation due to spleen deficiency that impair qi. If one want to have children,health-preservation is the most important. It could cleanse one’s heart and limit one’s desires,clam the psychological state,and not excessively think and consume mind-spirit；it should avoid excessive happy and anger,pay attention to temperance and essence nourishment,and make sure to enjoy ease of mind. The second is the combination of health-preservation and seed drugs therapy. The key of seed drug treatment is restoring normal coordination between heart and kidney,but not only tonifying kidney. The drugs of nourishing heart for tranquillization and relieving depression can be used,or even it is only emphasized the treatment principle of nourishing heart for tranquilizing and sedating mind,dispersing stagnated liver qi for relieving qi stagnation. The antidepressant western medicine shows the adverse effects on sexual function,so the therapy of traditional Chinese medicine presents certain advantages in the treatment of infertility due to depression syndrome.关键词：depression syndrome;infertility;etiology and pathogenesis;internal injury caused by excess of seven emotions
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发布时间：2022-08-31 - 摘要：Objective:To discuss the relationship between local syndrome factors of knee joints and tongue parameters in patients with knee osteoarthritis. Methods The local Chinese medical symptoms of knee joints in 110 patients with knee osteoarthritis were investigated through cross-sectional study. The local syndrome factors were summarized through clustering analysis,and the tongue nature and tongue coating parameters were collected through tongue module in tongue and pulse information collection and constitution identification system,and then the relationship between local syndrome factors of knee joints and tongue parameters was discussed. Results:①According to the clustering analysis,the local syndrome factors of knee joints could be divided into the wind pattern,cold pattern and dampness pattern. ②There were statistically significant differences on the L value in the tip and root of tongue and the b value in the root of tongue between the cold-dampness group and the normal group without wind,cold and dampness (P<0.05). There was statistically significant difference on the b value in the middle of tongue coat and root of tongue between the cold-dampness group and the normal group without wind,cold and dampness (P<0.05). The results indicated that for the patients of knee osteoarthritis with cold-dampness patterns,the tongue color in the tip and root of tongue was lighter and brighter,and the color in the middle and root of tongue coating was heavier,with a certain tendency of heat-transmission. Conclusion:There is a strong correlation between the local syndrome factors of knee joints and the overall tongue parameters,especially the parameters in the middle and root of tongue,which can provide certain objective basis for the clinical differentiation and treatment of knee osteoarthritis.关键词：knee osteoarthritis;syndrome factor;parameters of tongue manifestation;clustering analysis
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发布时间：2022-08-31 - 摘要：This article introduces Professor Li Fazhi’s experience in treating allergic rhinitis due to deficient cold with Chuanjiao Powder. It is considered that the pathogenesis of this disease is yang deficiency of spleen and kidney and wind-cold attacking lung. Chuanjiao Powder shows the effects of tonifying spleen and kidney and expelling cold and dispersing lung qi. Combined with medical cases,the key points of syndrome differentiation and medication experience according to symptoms are summarized.关键词：Chuanjiao Powder;allergic rhinitis due to deficient-cold;experience of famous doctor;Li Fazhi
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发布时间：2022-08-31 - 摘要：This paper summarizes Professor Chen Hongfeng’s experience in treating complex mastitis with acne based on quadruple external treatments,including incision and expansion- thread dragging- fumigation and washing- cotton pad. It is considered that this disease is caused by qi stagnation and blood coagulation,obstruction of meridians and collaterals,which leads to chronic stagnation transforming into heat. The heat induces the rotten skin and then it becomes abscess and easily forms fistula after fester. According to the different stages of the disease,the quadruple comprehensive treatments of “incision and expansion- thread dragging- fumigation and washing- cotton pad” can be applied. The application period,methods and attentions of each therapy are introduced. One proven case is presented.关键词：mastitis with acne;plasma cell mastitis;granulomatous mastitis;external treatment;experience of famous doctor;Chen Hongfeng
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发布时间：2022-08-31 - 摘要：This paper introduces Professor Cao Shili’s clinical experience in treating osteodynia of diabetic nephropathy with traditional Chinese medicine. At the beginning,the pathogenesis of the disease is dryness-heat due to yin deficiency which consumes qi and damages yin. As the disease progression,yin deficiency involves yang which leads to both deficiency of spleen and kidney. Until the advanced stage,it presents both deficiency of yin and yang,stasis and stagnation of vessels and meridians. The property of this disease is mainly deficiency in origin and excess in superficiality. The treatment methods include tonifying therapy that warming yang or nourishing yin to eliminating stagnation,therapy of promoting blood circulation for removing blood stasis by worm medicine,therapy of removing obstruction in collaterals according to symptoms,therapy of reinforcement and elimination in combination,and Niuxi(Achyranthis Bidentatae Radix)is made good use. One proven case is presented.关键词：diabetic nephropathy;osteodynia;therapy of traditional Chinese medicine;experience of physician;Cao Shili
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发布时间：2022-08-31 - 摘要：This article introduces Professor Liu Lingshuang’s experience in treating lung cancer in elderly by therapy of strengthening body resistance for treating cancer. It is considered that the etiology and pathogenesis are deficiency of vital qi,imbalance of yin and yang,decline of zang-fu viscera and invasion of pathogenic toxin. In clinical practice,the therapy of strengthening body resistance for treating cancer is taken as the principle,the treatment should balance yin and yang,invigorate spleen and tonify kidney and combine with accompanying symptoms. One prove case is presented.关键词：therapy of strengthening body resistance for treating cancer;lung cancer in elderly;experience;Liu Lingshuang
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发布时间：2022-08-31 - 关键词：classic formula;Huangqin Decoction;Dachaihu Decoction;Xiaochaihu Decoction;prescriptions of Chinese materia medica
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发布时间：2022-08-31 - 摘要：Combined with the specific cases of insomnia treatment in Ye Tianshi’s Linzheng Zhinan Yi’an(Guide to Clinical Practice with Medical Records),this paper summarizes Ye’s treatment on insomnia into six methods,including benefiting kidney water to restrict heart fire,relieving Shaoyang stagnated fire,nourishing spleen for tonifying deficiency,eliminating phlegm and fluid retention and dredging middle-jiao,filling gallbladder fluid to constrain dysphoria,invigorating liver and kidney for astringing floating yang.关键词：Ye Tianshi;insomnia;syndrome differentiation and treatment
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发布时间：2022-08-31 - 摘要：This paper discusses the potential connections between epigastric throb and autonomic nerves. Based on the description of epigastric throb in Shanghan Zabing Lun(Treatise on Cold Pathogenic and Miscellaneous Diseases),modern pharmacological researches on Chinese medicine for epigastric throb and modernization studies for abdominal throb by physicians of Chinese medicine in Japan,this paper analyzes the possible potential connections between epigastric throb and autonomic nerves from its symptoms,pharmacological mechanisms and modern researches. Epigastric throb may be related to the imbalance of autonomic nervous system with relative hyperactivity of sympathetic nerve and decreased vagus nerve tension.关键词：epigastric throb;autonomic nerves;relationship
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of “Chen’s No.9 Formula for kidney diseases” in the treatment of idiopathic membranous nephropathy. Methods60 patients with idiopathic membranous nephropathy were randomly divided into the treatment group and control group,30 cases in each group. The control group was treated with glucocorticoid plus cyclophosphamide and the treatment group was treated with“Chen’s No.9 Formula for kidney diseases”,with a course of 12 months. The clinical efficacy and the medication safety were observed. The changes on 24 h urine protein quantity,plasma albumin(Alb)and serum creatinine(Scr)were compared. Results:①The total remission rates were 76.7% in the treatment group and 59.3% in the control group. The clinical efficacy of the treatment group was obviously better than that of the control group (P<0.05). ②In both groups,there were statistically significant differences on the levels of 24 h urine protein quantity and Alb between treatment before and after (P<0.05),while there was no statistically significant difference on the Scr level between treatment before and after(P>0.05). After treatment,the level of 24 h urine protein quantity in the treatment group was obviously lower than that in the control group (P<0.05),while the Alb level in the treatment group was obviously higher than that in the control group (P<0.05). ③During the trial,there was statistically significant difference on the incidence rate of adverse reactions between the two groups,the incidence rate of adverse reactions in the treatment group was obviously lower than that in the control group(P<0.05). Conclusion:“Chen’s No.9 Formula for kidney diseases” shows satisfactory efficacy in the treatment of idiopathic membranous nephropathy with fewer adverse reactions,especially for the patients who are not sensitive to routine western medicine.关键词：idiopathic membranous nephropathy;“Chen’s No.9 Formula for kidney diseases”;benefiting qi and activating blood circulation and resolving dampness;specific formula for specific disease;Shuizhi(Hirudo)
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of Baogui Capsules combined with western medicine in the treatment of polycystic ovarian syndrome and its effects on anti-mullerian hormone(AMH). Methods68 patients with polycystic ovarian syndrome were randomly divided into the control group and treatment group,34 cases in each group. The control group was treated with ethinylestradiol and cyproterone acetate tablets,and the treatment group was treated with Baogui Capsules based on the treatment for the control group,with a course of 3 menstrual cycles. The patients were followed-up for 3 months. The efficacy on menstrual disorder was observed. The changes on AMH level and serum levels of estradiol(E2),follicle stimulating hormone(FSH),luteinizing hormone(LH)and total testosterone(T)were compared. Results:①The clinical total effective rates were 85.3% in the treatment group and 58.8% in the control group. The efficacy on menstrual disorder in the treatment group was obviously better than that in the control group (P<0.05). ②In both groups,there was statistically significant difference on the AMH level between treatment before and 3 months after drug withdrawal (P<0.05). 3 months after drug withdrawal,there was statistically significant difference on the AMH level between the two groups,and the AMH level in the treatment group was significantly lower than that in the control group (P<0.05). ③In both groups,there were statistically significant differences on the levels of LH,LH/FSH and T between treatment before and 3 months after drug withdrawal (P<0.05),while there were no statistically significant differences on the levels of E2 and FSH(P>0.05). 3 months after drug withdrawal,there was statistically significant difference on the T level between the two groups (P<0.05). Conclusion:Compared with western medicine alone,Baogui Capsules combined with western medicine can more effectively improve the menstrual disorder in the treatment of polycystic ovary syndrome,and reduce the serum T and AMH levels.关键词：polycystic ovarian syndrome;Baogui Capsules;Diane-35;anti-mullerian hormone;ovulation disorder;hyperandrogenemia
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of Huayu Sanjie Enema in the treatment of adenomyosis with syndrome of blood stasis due to burning heat. Methods62 patients of adenomyosis with syndrome of blood stasis due to burning heat were randomly divided into the treatment group(32 cases)and control group(30 cases). The treatment group was treated with Huayu Sanjie Enema and the control group was treated with desogestrel and ethinylestradiol tablets,with a course of 3 menstrual cycles. The changes on the scores of dysmenorrhea,menstrual quantity and TCM syndrome,the uterine size and the level of serum carbohydrate antigen(CA)125 were observed and compared. Results:①In both groups,there were statistically significant differences on the scores of dysmenorrhea,menstrual quantity and TCM syndrome between treatment before and after (P<0.05). After treatment,the TCM syndrome score in the treatment group was significantly lower than that in the control group (P<0.05),while there were no statistically significant differences on the scores of dysmenorrhea and menstrual quantity between the two groups(P>0.05). ②In both groups,there was statistically significant difference on the uterine size between treatment before and after (P<0.05). For the comparison between treatment before and after,there was statistically significant difference on the CA125 level in the treatment group (P<0.05),while there was no statistically significant difference in the control group(P>0.05). After treatment,the uterine size in the treatment group was obviously smaller than that in the control group (P<0.05),and the CA125 level in the treatment group was obviously lower than that in the control group (P<0.05). Conclusion:Huayu Sanjie Enema shows satisfactory efficacy in the treatment of adenomyosis with syndrome of blood stasis due to burning heat,which can obviously relieve the clinical symptoms and signs of patients.关键词：adenomyosis;syndrome of blood stasis due to burning heat;Huayu Sanjie Enema;oral contraceptives;rectal administration
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of Sanhuang Jiedu Decoction combined with metronidazole in the treatment of acute pericoronitis,and the influence on the periodontal microflora,erythrocyte sedimentation rate(ESR)and C-reactive protein(CRP). Methods114 patients with acute pericoronitis were randomly divided into the control group and treatment group,57 cases in each group. The control group was treated with metronidazole powder after rinsing the wisdom tooth crown and blind bag,and the treatment group was treated with sponge dipped Sanhuang Jiedu Decoction based on the treatment for the control group,with a course of 3 days. The clinical efficacy was observed,the changes on traditional Chinese medicine(TCM)syndrome score,periodontal microflora,ESR and CRP were compared. Results:①Compared with treatment before,the TCM syndrome score was decreased in both groups after treatment (P<0.05). After treatment,the TCM syndrome score in the treatment group was significantly lower than that in the control group (P<0.05). ②The total effective rates were 98.25% in the treatment group and 85.96% in the control group,there was statistically significant difference on the clinical efficacy between the two groups (P<0.05). ③Compared with treatment before,the proportion of gram-positive(G+)bacteria was increased in both groups after treatment (P<0.05),while the proportion of gram-negative(G-)bacteria and spirochete and the bacteria value were decreased in both groups after treatment(P<0.05). After treatment,there were statistically significant differences on the proportion of G+,G- and spirochete and the bacteria value between the two groups (P<0.05). ④Compared with treatment before,the levels of ESR and CRP were decreased in both groups after treatment (P<0.05). After treatment,the levels of ESR and CRP in the treatment group were significantly lower than those in the control group (P<0.05). Conclusion:Sanhuang Jiedu Decoction combined with metronidazole shows good efficacy in the treatment of acute pericoronitis,which can significantly improve the clinical symptoms of patients,regulate the periodontal microflora and control the inflammation.关键词：Sanhuang Jiedu Decoction;metronidazole;acute pericoronitis;periodontal microflora
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of massage and acupoint pressure in the treatment of spastic hemiplegia of upper limb after cerebral infarction. MethodsEighty patients with spastic hemiplegia of upper limb after cerebral infarction were randomly divided into the treatment group and control group,40 cases in each group. The control group was treated with routine rehabilitation training,and the treatment group was treated with acupoint pressure based on the treatment for the control group,with a course of 21 days. The changes on the FMA scale,BI scale and upper limb electromyography were observed. Results:After treatment,the FMA score and the BI score were significantly increased in both groups(P<0.05),and there were statistically significant differences on the FMA score and BI score between the two groups(P<0.05). After treatment,the ratio of Hmax/Mmax were significantly decreased in both groups(P<0.05). For the D-value of Hmax/Mmax before and after treatment,there was statistically significant difference between the two groups(P＜0.05). Conclusion:Therapy of massage and acupoint pressure shows definite efficacy in the treatment of spastic hemiplegia of upper limb after cerebral infarction,which can play the positive effects on improving limb spasticity and promoting nerve function recovery.关键词：cerebral infarction;spastic hemiplegia of upper limb;massage;acupoint pressure
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of intra-articular injection of chitosan combined with manipulation in the treatment of degenerative knee osteoarthritis. Methods120 patients were randomly divided into the control group and treatment group,60 cases in each group. The control group was only treated with chitosan by intra-articular injection,the treatment group was treated with manipulation plus chitosan by intra-articular injection,with a course of 5 weeks. The clinical efficacy and the changes of WOMAC scores were observed. Results:The total effective rates were 95.0% in the treatment group and 88.3% in the control group,and the clinical efficacy of the treatment group was better than that of the control group(P<0.05). After treatment,the scores of knee pain,stiffness and joint function were significantly decreased in both groups(P<0.05),and the reductions of stiffness and joint function scores in the treatment group were greater than those in the control group(P<0.05). Conclusion:The intra-articular injection of chitosan combined with manipulation shows good clinical efficacy in the treatment of degenerative knee osteoarthritis,which is safe and reliable. Compared with intra-articular injection alone,it can better improve the knee stiffness and joint function of patients.关键词：degenerative knee osteoarthritis;injection therapy;manipulation;clinical research
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发布时间：2022-08-31 - 摘要：Objective:To observe the efficacy of hemisection and ligation surgery of lateral anal internal sphincter in the treatment of chronic anal fissure. Methods60 patients with chronic anal fissure were randomly divided into the treatment group and control group,30 cases in each group. The control group was treated with anal internal sphincterotomy and the treatment group was treated with hemisection and ligation surgery of lateral anal internal sphincter. The postoperative pain and bleeding,wound area and wound healing time were observed and compared between the two groups. Results:①On the 1st,3rd and 7th day after the operation,the pain and bleeding scores in the treatment group were lower than those in the control group at the corresponding period (P<0.05). ②On the 1st,3rd,7th and 14th day after the operation,the wound area score in the treatment group was lower than that in the control group at the corresponding period (P<0.05). ③There was statistically significant difference on the wound healing time between the two groups (P<0.05). Conclusion:The hemisection and ligation surgery of lateral anal internal sphincter in the treatment of chronic anal fissure can reduce the bleeding and relieve the pain after surgery,diminish the wound area and shorten the wound healing time.关键词：anal fissure;internal sphincter;seton;ligation
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发布时间：2022-08-31 - 摘要：This paper discusses the relationship between stagnation of collaterals due to yang deficiency and gouty. It is considered that stagnation of meridian and collaterals due to yang deficiency is one of the most important pathogenesis of gouty. Yang deficiency failing to warm causes cold-dampness accumulating in the interior,yang deficiency failing to warm leads to intermingled phlegm and blood stasis,yang deficiency induces dampness and blood stasis,which easily transforms into heat when long stagnated. For gouty due to yang deficiency,the root cause of the disease is yang deficiency of spleen and kidney,and the excess in superficiality is dampness,phlegm and blood stasis obstructing meridians and collaterals. The treatments should take therapy of warming yang and dredging collaterals as principle. One proven case is presented.关键词：gouty;Bi syndrome;yang deficiency;stagnation of collaterals;warming yang and dredging collaterals
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发布时间：2022-08-31 - 摘要：Objective:To study the inhibitory effects of Changweiqing on chemotherapy induced lung metastasis of colon cancer in mice and explore its mechanism. MethodsThe chemotherapy induced lung metastasis of colon cancer model was established by one-time intraperitoneal injection of oxaliplatin(L-OHP)at maximum tolerable dose and intravenous injection of colon cancer CT26 cells via caudal vein 3 days later. 24 BALB/c mice were randomly divided into the model group,chemotherapy induced lung metastasis(L-OHP)group,Changweiqing plus chemotherapy induced lung metastasis(CWQ+L-OHP)group. The nodules number of lung metastasis was counted,the tumor lesions in the lung were detected by HE staining,the microvessel density(MVD)of lung metastatic tumor tissue was detected by immunohistochemistry,and the protein and mRNA expressions of vascular endothelial growth factor(VEGF)-A,matrix metalloproteinase 2/9(MMP2/9)in the lung metastasis were detected by Western blot and qRT-PCR. Results:Compared with the L-OHP group,the nodules number of lung metastasis was reduced in the CWQ+L-OHP group(P<0.05),and the MVD value of tumor tissue was decreased(P<0.01),and the protein and mRNA expressions of VEGF-A,MMP2 and MMP9 in the lung tissue were all down-regulated(P<0.01). Conclusion:Changweiqing can inhibit chemotherapy induced lung metastasis of colon cancer in mice. Its mechanism may be related to down-regulating the expressions of VEGF-A,MMP2 and MMP9 in tumor tissue and reducing MVD and tumor angiogenesis.关键词：Changweiqing;chemotherapy;colon cancer;lung metastasis;vascular endothelial growth factor;matrix metalloproteinase
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发布时间：2022-08-31 - 摘要：Objective:To discuss the effects and possible molecular mechanisms of artesunate on the cell apoptosis of human gastric cancer line HGC27. Methods The HGC27 cells were divided into the control group,artesunate groups at concentrations of 20 mg·L-1,80 mg·L-1 and 160 mg·L-1,respectively. The effects of artesunate on HGC27 cell proliferation were detected by CCK8 assay after treatment for 24 h,48 h and 72 h,respectively. The effects of artesunate on HGC27 cell apoptosis were detected by flow cytometry. The effects of artesunate on the expressions of β-catenin,glycogen synthase kinase-3β(GSK-3β),c-Myc and cysteinyl aspartate specific proteinase-3(Caspase-3)were detected by Western blot. Results:Artesunate could inhibit the HGC27 cell proliferation. Compared with the control group,the inhibitory rate of cell proliferation was significantly increased in artesunate groups at all concentrations(P<0.05),with a concentration- and time-dependent manner. Artesunate could induce the HGC27 cell apoptosis. Compared with the control group,the rate of cell apoptosis was significantly increased in artesunate groups at all concentrations(P<0.05),with a concentration-dependent manner. The results of Western blot showed that the protein expression levels of GSK-3β and Caspase-3 were significantly up-regulated(P<0.05),while those of β-catenin and c-Myc were significantly down-regulated(P<0.05)in artesunate-treated groups. Conclusion:Artesunate can significantly inhibit the proliferation and induce the apoptosis of HGC27 cells,which may be related to inhibiting the activation of Wnt/β-catenin signaling pathway.关键词：artesunate;human gastric cancer cell;cell apoptosis;Wnt/β-catenin signaling pathway
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发布时间：2022-08-31 - 摘要：Objective:To observe the intervention effects of Chinese medical manipulation on the articular cartilage degeneration in rabbit model of knee osteoarthritis,and explore its mechanism from the perspective of cell signaling transduction. Methods24 New Zealand white rabbits were randomly divided into the normal group,model group and manipulation group. Except the normal group,the model of knee osteoarthritis was established by operation in the other two groups. After modeling for 4 weeks,the manipulation group was treated with manipulation therapy such as patella massage and joint passive flexion and extension. After intervention for 8 weeks,the rabbits were sacrificed and the knee joints were exposed for morphological observation. The femoral condyle was collected and made into pathological sections for HE staining. The histopathological features were observed under microscope and evaluated by Mankin’s score,and the degeneration of articular cartilage in each group was compared. The mRNA expression levels of IL-1β、iNOS and MMP-13 in synovial tissues and P38 as well as ERK1/2 in cartilage tissue were detected by RT-PCR. The protein expression levels of MMP-13 and phosphorylated ERK1/2(p-ERK1/2)in cartilage tissue were detected by Western-blot. All the experimental data were compared and conducted the statistical analysis. Results:The degree of articular cartilage degeneration in the manipulation group was obviously alleviated compared with the model group,and the Mankin’s score in the manipulation group was significantly lower than that in the model group (P<0.05). Compared with the model group,the mRNA expression levels of P38 and ERK1/2 in cartilage and IL-1β,iNOS and MMP-13 in synovial tissues in the manipulation group were significantly down-regulated (P<0.05). Compared with the normal articular cartilage,the protein expressions of MMP-13 and p-ERK1/2 were significantly up-regulated in the model group and manipulation group. While the protein activation degree of MMP-13 and p-ERK1/2 in the manipulation group was obviously inhibited compared with that in the model group. Conclusion:Chinese medical manipulation can effectively inhibit the cartilage degeneration degree of knee osteoarthritis,and affect the expressions of inflammatory mediators and cytokines. Its mechanism may be associated with inhibition of mitogen-activated protein kinase(MAPK)signal transduction pathway in articular cartilage.关键词：knee osteoarthritis;manipulation;articular cartilage;mitogen-activated protein kinase;signaling pathway
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发布时间：2022-08-31 - 摘要：Objective: To observe the effects of external application of Hongzhongxiao Tincture,a drug for encircling lesion,on the C reactive protein(CRP)and procalcitonin(PCT)in plasma and matrix metalloproteinase-2(MMP-2)in secretion and the histopathology at different locations around the abscess in rats with skin abscess under the guidance of defended field theory. Methods40 rats were divided into the blank group,pretreatment group,treatment group,control group and model group. Except the blank group,the subcutaneous abscess model was established by subcutaneous injection of Staphylococcus aureus in the other four groups. After modeling,the rats were treated with different drugs. On the 3rd,7th and 14th day of treatment,the cardiac blood at volume of 1.5 ml was collected in each group. Except the rats in the control group,100 μl 0.9% NaCl solution was used for other rats to rinse the wound and then the wound secretion was recycled. The levels of CRP and PCT in plasma and MMP-2 in secretion were detected by enzyme linked immunosorbent assay(ELISA). The rats were sacrificed after treatment for 14 days,and the subcutaneous tissues at 1 cm,2 cm and 3 cm around abscess were extracted respectively and made into sections for Masson staining. The inflammatory cell infiltration,vascular proliferation and telangiectasia in skins and muscles at different locations around abscess were observed under the microscope. Results:In different treatment periods,the plasma levels of CRP and PCT in the model group,control group,pretreatment group and treatment group showed the decreasing tendency and were higher than those in the blank group,and tended to be stable at the end of the treatment. During the treatment periods,the plasma levels of CRP and PCT in the pretreatment group and treatment group(both treated with Hongzhongxiao Tincture by external application)were lower than those in the control group and model group to varying degrees. In the early stage of the treatment(3rd and 7th day),there were statistically significant differences on the plasma levels of CRP and PCT between the control group(treated with Mupirocin Ointment by external application)and pretreatment group or treatment group(P<0.05,P<0.01). On the 14th day of treatment,the plasma levels of CRP and PCT in the pretreatment group and treatment group were close to those in the control group,with no statistically significant differences(P>0.05). In the middle and late stage of the treatment(7th and 14th day),the MMP-2 level in wound secretion in the pretreatment group and treatment group was lower than that in the model group and control group,with statistically significant differences(P<0.05,P<0.01). The pathology of tissues around abscess under the microscope showed that the inflammatory cells infiltration in the subcutaneous muscle at different locations around abscess in the treatment group and pretreatment group was significantly lower than that in the control group and model group. Conclusion:The therapy of encircling lesion with Hongzhongxiao Tincture is applied in the treatment of rat skin abscess,which can alleviate inflammatory infiltration in tissue around abscess and improve the systemic and local infection by reducing the levels of CRP and PCT in plasma and MMP-2 level in secretion,and thus provide favorable conditions for the formation of defended field.关键词：defended field theory;therapy of encircling lesion with drugs;abscess;inflammatory factors;rat
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发布时间：2022-08-31 - 摘要：Objective:To discuss the therapeutic effects of echinacoside on lead acetate induced dyszoospermia in mice. Methods60 male BALB/c mice were randomly divided into the control group,model group,positive drug group and echinacoside groups with low-,middle- and high-dose,10 mice in each group. Except the control group,the mouse model of dyszoospermia was established by intraperitoneal injection of lead acetate at daily dose of 20 mg/kg for continuous 7 days in the other groups. After successful modeling,the positive drug group was treated with human chorionic gonadotropin(HCG)by intraperitoneal injection at daily dose of 500 U/kg. The echinacoside groups with low-,middle- and high-dose were treated with echinacoside by intragastric administration at doses of 25 mg/kg,50 mg/kg and 100 mg /kg,respectively. The control group and the model group were treated with 0.9% NaCl solution by intragastric administration at equivalent volume,with a course of 30 days. 24 hours after the last intragastric administration,the epididymis was separated,the sperm density,sperm motility rate and teratospermia rate were observed. The testicular tissue was fixed to observe the histopathological changes. The serum was separated and the testicular tissue was homogenized,the level of testosterone(T)in testicular tissue and the levels of luteinizing hormone(LH)and follicle stimulating hormone(FSH)in serum were detected by enzyme linked immunosorbent assay. Results:Compared with the control group,the sperm density and the sperm motility rate were decreased and the teratospermia rate was increased in the model group,and the T level in the testicular tissue homogenate was decreased and the levels of FSH and LH in serum were increased,with statistically significant differences(P<0.01). Compared with the model group,the sperm density and the sperm motility rate were increased and the teratospermia rate was decreased in the echinacoside groups with different doses,and the levels of FSH and LH in serum were decreased and the T level in the testicular tissue homogenate was increased,with statistically significant differences(P<0.01). The therapeutic effects of echinacoside on the reproductive impairment were in a dose-dependent manner. The histological observation showed that the pathological injuries in the testicular tissue were improved in echinacoside groups with different doses. Conclusion:Echinacoside shows obvious therapeutic effects on lead acetate induced dyszoospermia in mice,and its mechanisms may be associated with improving the reproductive hormone levels and recovering the regulation function of hypothalamus-pituitary-gonad axis.关键词：echinacoside;lead acetate;spermatogenesis;reproductive hormone;mouse
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发布时间：2022-08-31 - 摘要：Objective:To determinate and compare the content of ferulic acid in root bark and root xylem of Xinjiang Berberis heteropda Schrenk by HPLC,and optimize the extraction process conditions. Methods①The root bark and root xylem of Berberis heteropoda Schrenk were extracted with different solvents,and the optimum extraction solvent was selected according to the results of contents determination. ②Based on the results of single factor test,the extraction process of ferulic acid was optimized by response surface methodology. Results:①The content of ferulic acid in the ethanol extraction was the highest. ②The optimum extraction conditions were confirmed after three times parallel test. The extraction efficiency was maximum when the extraction solvent was 60% ethanol,the extraction temperature was 50 ℃,the liquid ratio was 1:15 and the times of extraction were 3. The extraction amount of ferulic acid was 0.71%(RSD=1.24%)in the root bark and 0.32%(RSD=1.51%)in the root xylem. Conclusion:This extraction process is stable and feasible with higher extraction efficiency.关键词：root bark of Berberis heteropda Schrenk;root xylem of Berberis heteropoda Schrenk;ferulic acid;response surface methodology;content determination
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发布时间：2022-08-31 - 摘要：This paper reviews the research progress on influence of alkaloids compounds on central nervous system. Alkaloids show the therapeutic and neuroprotective effects on diseases of central nervous system,such as Alzheimer’s disease,Parkinson’s disease,depression,anxiety,convulsion,epilepsy. And the development prospect of their clinical treatment is also forecasted.关键词：alkaloids;central nervous system;Alzheimer’s disease;review
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发布时间：2022-08-31
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