最新刊期
期 5 ， 2018
- 摘要：This paper introduces national Chinese medical science master Lu Fang’s experience in treating autoimmunity skin lesions with Yimian Decoction. It is considered that the pathogenesis of this disease is disharmony of Ying and Wei,dry and rotten skin. Yimian Decoction can harmonize yingfen and weifen,clear heat and cool blood,promote fluid production and dissipate masses,remove toxicity and dredge channels. The modified recipes according to symptoms should pay attention to the etiology-based medication and application of channel ushering drug of skin. One proven case is presented.关键词：autoimmunity;skin lesions;Yimian Decoction;experience of famous doctor;Lu Fang
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发布时间：2022-08-31 - 摘要：This paper analyzes the misunderstandings,etiology and strategy of drug treatments on gastroesophageal reflux disease at present. It is considered that the main problem of gastroesophageal reflux disease is not excessive secretion of gastric acid,but digestive tract dyskinesis induced acid dislocation,which caused by dysfunction of lower esophageal sphincter,disorder of esophageal acid clearance and gastrointestinal emptying delay. The ultimate goal of treatment should be to adjust the gastrointestinal motility to promote downward. The therapy of acupuncture and moxibustion shows good regulation on the gastrointestinal motility and lower esophageal sphincter,and presents the significant advantages in the treatment of gastroesophageal reflux disease.关键词：gastroesophageal reflux disease;acid regurgitation;acid dislocation;therapy of acupuncture and moxibustion
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发布时间：2022-08-31 - 摘要：Through the comprehensive review on ancient literatures,it finds that spermatorrhea mainly belongs to the category of depression syndrome. The main emotional causes lead to spermatorrhea include indulging oneself in carnal pleasure without restraint,excessive worry,psycholagny,depression of liver qi,hyperactivity of sovereign fire,overthinking and so on. In essence,spermatorrhea is a kind of heart disease,and the stagnation of liver qi and worry impairing spleen are the common pathogenesis. Kidney deficiency and kidney dysfunction in essence storage are two different concepts. Kidney deficiency results from chronic and serious illness,and may not appear spermatorrhea caused by dysfunction of essence storage；while kidney dysfunction in essence storage must be due to emotional factors,and lead to spermatorrhea through disharmony between heart and kidney and linkage mechanism of heart-liver-kidney. The common concomitant symptoms of spermatorrhea are mostly characterized by depression syndrome. In ancient times,the therapy for spermatorrhea due to depression syndrome was treated from the heart,and even emphasized clearing mind which could recover the illness without medicine. Until nowadays,more and more doctors have treated spermatorrhea from the liver. In the diagnosis and treatment of spermatorrhea,doctors should not only tonify kidney,but also be conscious of spermatorrhea due to depression syndrome.关键词：spermatorrhea;depression syndrome;relieving depression
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发布时间：2022-08-31 - 摘要：Objective:To establish the rat knee osteoarthritis model by partial patellar ligament defect combined with medial collateral ligament transection. Methods30 SD rats were randomly divided into the blank group, 3-week model group and 6-week model group, 10 rats in each group. The rats in the 3-week model group and 6-week model group were performed the operation to induce the partial patellar ligament defect and medial collateral ligament transection. The rats in the blank group were only cut the skin to expose the patellar ligament and medial collateral ligament, without other surgical treatment. One week after surgery, the rats were forced to exercise for 30 minutes per day. 3 weeks and 6 weeks after modeling,the rats were sacrificed respectively,and the knee joint was dissected. The articular surface of the patella and the femoral condyle were exposed to observe the morphological changes,and the cartilage morphology of femoral condyle articular surface was evaluated by Pelletier’s principle. The samples of knee joint were collected,and the pathological sections were stained with safranin O-fast green and Alcian blue to observe the morphological structure of cartilage by light microscopy,and the degree of degeneration was evaluated by modified Mankin’s score. Results:The degeneration of knee joints with different degrees were observed in the 3-week and 6-week model groups,and the Pelletier score and modified Mankin’s score in these two groups were higher than those in the blank group (P<0.05),and the scores in the 6-week model group were higher than those in the 3-week model group (P<0.05). Conclusion:The rat knee osteoarthritis model can be effectively established by the partial patellar ligament defect combined with medial collateral ligament transection. The histological changes are consistent with the pathological features of osteoarthritis cartilage degeneration,and the operation is simple with small traumas and low infection rates,which is an ideal modeling method.关键词：knee osteoarthritis;disease model;rat
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发布时间：2022-08-31 - 摘要：This paper introduces Professor Liu Sheng’s clinical experience in the treatment of granulomatous mastitis. It is considered that the pathogenesis of this disease is liver qi depression transforming into fire and turbidity due to spleen deficiency. The therapy is based on resolving method and the liver and spleen are emphasized. The treatments should perform by stages and focus on local part. The internal treatment is taken as the principal thing and the external treatment is taken as the supplement. Recovery is important to reinforce. For pregnancy,it could nourish yin and clear heat,expel toxin and prevent miscarriage.关键词：granulomatous mastitis;clearing and resolving method;experience of physician;Liu Sheng
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发布时间：2022-08-31 - 摘要：This paper introduces Professor He Dongyi’s five methods in the treatment of rheumatoid arthritis. It is considered that rheumatoid arthritis is a syndrome of deficiency in origin and excess in superficiality. The treatment should focus on tonifying kidney to treat the root cause. The therapy of promoting blood circulation for removing blood stasis and dredging collateral for relieving rheumatism should be applied throughout the course of disease. Protecting spleen and stomach and preventing disease before disease onset are proposed. Meanwhile,taking internal and external treatment in combination is emphasized,the external or exercise therapies include Qin’s Xiaobi Ointment,joint health exercise and so on. The couplet medicines are made good use to eliminate pathogen. The pharmacological mechanism of Tripterygium wilfordii,a herb drug of anti-rheumatism with toxicity,is deeply explored.关键词：rheumatoid arthritis;promoting blood circulation for removing blood stasis;dredging collateral for relieving rheumatism;experience of famous doctor;He Dongyi
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发布时间：2022-08-31 - 摘要：This paper introduces Professor Wang Jing’s clinical experience in treating chest painful impediment from phlegm and blood stasis due to qi deficiency. It is considered that the pathogenesis of chest painful impediment is qi deficiency,phlegm and blood stasis. In the treatment,it should pay attention to the syndrome differentiation of qi and blood. The therapy of invigorating qi and nourishing blood is emphasized,and combined with therapies of promoting blood circulation for removing blood stasis and dissipating phlegm and removing dampness,which treat both manifestation and root cause of disease and take dredging and tonifying in combination. One proven case is presented.关键词：chest bi;chest pain;experience of famous doctor;Wang Jing
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发布时间：2022-08-31 - 关键词：melanephidrosis;asthma and oppression in chest;vertigo;Erjia Longgu Decoction;corresponding of prescription and syndrome
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发布时间：2022-08-31 - 摘要：Based on Zhang Zhongjing’s view of “slight sweating all over the body” in Shanghan Lun(Treatise on Cold Pathogenic Diseases),this paper discusses the application of slight sweating method in Shanghan Lun,including the key principle and theory,methods and aims,dosage and decocting method,medication instruction and contraindication,and explains its influence on later generation and the modern clinical application.关键词：slight sweating method;Shanghan Lun(Treatise on Cold Pathogenic Diseases);application;influence
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发布时间：2022-08-31 - 摘要：Ge Hong creates the unique thought of physique-spirit based on Lao-Zhuang’s thoughts. It is considered that physique takes spirit as the base,and spirit takes physique as the manifestation. The health preservation of physique-spirit emphasizes the noble morality,firm belief,harmony of body and mind,combination of dynamics and statics. Inspired by the thought of physique-spirit,the important contents of psychosomatic medicine should include carrying forward the concept of medical humanitarianism,deepening the thought of preventive treatment of disease and exploring its foundation of traditional culture.关键词：Ge Hong;physique-spirit thought;health preservation;psychosomatic medicine
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of Buxu Ruangan Decoction in the treatment of primary biliary cirrhosis with poor response to ursodeoxycholic acid. MethodsSixty-two patients of primary biliary cirrhosis with poor response to ursodeoxycholic acid were randomly divided into the treatment group (n=32) and control group (n=30). The control group was treated with routine therapy,and the treatment group was treated with Buxu Ruangan Decoction based on the treatment for the control group,with a course of 24 weeks. The clinical efficacy was observed,and the changes on the chronic liver disease questionnaire (CLDQ) score,liver function indexes,liver fibrosis indexes and fibroscan test were compared. Results: ①The total effective rates was 87.5% in the treatment group and 63.3% in the control group,and the clinical efficacy of the treatment group was better than that of the control group(P＜0.05). ②After treatment,the CLDQ score was decreased in both groups(P＜0.05),and there was statistically significant difference on the CLDQ score between the two groups(P＜0.05). ③After treatment,the levels of ALT,AST,TBIL,GGT,ALP and TBA were decreased in both groups(P＜0.05),and there were statistically significant differences on the levels of all liver function indexes between the two groups(P＜0.05). ④After treatment,the levels of HA,LN,PC-Ⅲ and Ⅳ-C and the value of liver stiffness measurement(LSM)were decreased in both groups(P＜0.05),and the levels of HA and Ⅳ-C and the LSM value in the treatment group were lower than those in the control group(P＜0.05). Conclusion:Buxu Ruangan Decoction shows good clinical efficacy in the treatment of primary biliary cirrhosis with poor response to ursodeoxycholic acid,which can improve the liver function and liver fibrosis indexes,decrease the LSM value and enhance the quality of life.关键词：Buxu Ruangan Decoction;ursodeoxycholic acid;primary biliary cirrhosis
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发布时间：2022-08-31 - 摘要：Objective:To observe the effects of traditional Chinese medicine (TCM) clinical pathway on life quality of postoperative patients of colorectal cancer with spleen deficiency. Methods60 postoperative patients of colorectal cancer with spleen deficiency were randomly divided into the pathway group and non-pathway group, 30 cases in each group. The patients in the non-pathway group were treated according to the symptoms, and the patients in the pathway group were treated according to the established TCM clinical pathway, with a course of 12 weeks. The changes on TCM syndrome score and EORTCQLQ-C30 scale score were compared. Results:①Compared with treatment before, the TCM syndrome score was decreased in the pathway group after treatment (P<0.01), but no obvious change was observed in the non-pathway group (P>0.05). After treatment, there was statistically significant difference on the TCM syndrome score between the two groups (P<0.01). ②Compared with treatment before, the scores of physical and cognitive function in function subscale were increased in the pathway group after treatment (P<0.01), and no obvious changes on all the scores were observed in the non-pathway group (P>0.05). After treatment, there were statistically significant differences on the physical and cognitive function scores between the two groups (P<0.01). ③Compared with treatment before, the scores of fatigue, nausea and vomiting, dyspnea and diarrhea in symptom subscale were decreased in the pathway group after treatment (P<0.05), while only the symptom score of dyspnea was decreased in the non-pathway group (P<0.05). After treatment, the improvements on fatigue, nausea and vomiting, diarrhea and appetite in the pathway group were better than those in the non-pathway group (P<0.01). ④Compared with treatment before, the score of general health subscale was increased in the pathway group (P<0.01) and decreased in the non-pathway group (P<0.01) after treatment. After treatment, there was statistically significant difference on the score of general health subscale between the two groups (P<0.01). Conclusion:The TCM clinical pathway can improve the clinical symptoms and enhance the life quality of postoperative patients of colorectal cancer with spleen deficiency.关键词：colorectal cancer;postoperation;spleen deficiency syndrome;clinical pathway;life quality
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of modified Erxian Decoction combined with Jinghuosu in the treatment of oligospermia and asthenospermia with deficiency of kidney yang. Methods105 patients of oligospermia and asthenospermia with deficiency of kidney yang were randomly divided into the combination group, Chinese medicine group and Jinghuosu group, 35 cases in each group. The combination group was treated with modified Erxian Decoction plus Jinghuosu, the Chinese medicine group was treated with modified Erxian Decoction and the Jinghuosu group was treated with Jinghuosu, with a course of 3 months. The clinical efficacy was observed, and the changes on semen routine indicators were compared. Results:①The clinical total effective rates were 62.9% in the Jinghuosu group, 65.7% in the Chinese medicine group and 82.9% in the combination group. The clinical efficacy of the combination group was significantly better than that of the Jinghuosu group (P<0.05) and the Chinese medicine group (P<0.05). There was no statistically significant difference on the efficacy between the Jinghuosu group and Chinese medicine group (P>0.05). ②In all groups, there were statistically significant differences on the semen concentration, sperm survival rate, proportion of a-grade sperm and proportion of (a+b)-grade sperm between treatment before and after (P<0.05). After treatment, there were statistically significant differences on the semen concentration and sperm survival rate between the Chinese medicine group and Jinghuosu group (P<0.05). The improvement on all the indicators in the combination group was significantly better than that in the Jinghuosu group (P<0.05) and the Chinese medicine group (P<0.05). Conclusion:The modified Erxian Decoction combined with Jinghuosu can significantly improve the semen quality and sperm motility in the treatment of oligospermia and asthenozoospermia with deficiency of kidney yang.关键词：oligospermia and asthenospermia;male infertility;syndrome of deficiency of kidney yang;modified Erxian Decoction;Jinghuosu
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of Ruyong Formula on lactational mastitis and its possible mechanism. Methods52 patients with lactational mastitis were treated with Ruyong Formula, with a course of one week. A comparative study was conducted between treatment before and after and between bilateral breasts. The changes on the scores of clinical symptoms (such as breast lump, breast distending pain, obstructed milk, local hot breast skin, aversion to cold with fever) were observed and compared before and after treatment. And the pathogenic bacteria and probiotics in milk of bilateral breasts were qualitatively and quantitatively determined. Results:①Compared with treatment before, the breast lump was obviously reduced (P<0.05), the breast distending pain was obviously alleviated (P<0.05), the obstructed milk was obviously improved (P<0.05), the local hot breast skin was obviously relieved (P<0.05) and the aversion to cold with fever was obviously improved (P<0.05) after treatment. ②After treatment, the amount of pathogenic bacteria was significantly reduced in the milk of the affected side (P<0.05). ③Before treatment, there were no statistically significant differences on the quantitative Ct values of Straphylococcus, Streptococcus, Bifidobacteria and Lactobacilli in breast milk between the affected side and healthy side (P>0.05). ④For the Straphylococcus-infected patients, on the same side of breast (affected or healthy side), there were statistically significant differences on Ct values of Straphylococcus, Bifidobacteria and Lactobacilli between treatment before and after (P<0.05). For the comparison of D-value on flora Ct values between treatment before and after, the D-value on Ct value of Straphylococcus was increased more obviously on the affected side, which meant that the amount of Straphylococcus was reduced more significantly on the affected side (P<0.05). The D-value on Ct value of Bifidobacteria was decreased more obviously on the affected side, which meant that the amount of Bifidobacteria was increased more significantly on the affected side (P<0.05). For the comparison of D-value on Ct value of Lactobacilli between treatment before and after, there was no statistically significant difference between the affected side and healthy side (P>0.05). ⑤Only one case infected with Streptococcus was found in this study. In the milk of affected side, the amount of Streptococcus showed the decreasing trend and the amount of Bifidobacteria and Lactobacilli showed the increasing trend. ⑥For the non-bacteria mediated patients, on the same side of breast (affected or healthy side), there was statistically significant difference on the Ct value of Bifidobacteria between treatment before and after (P<0.05), and there was no statistically significant difference on the Ct value of Lactobacilli between treatment before and after (P>0.05). For the comparison of D-value on Ct value of Bifidobacteria between treatment before and after, the Ct value of Bifidobacteria was decreased more obviously on the affected side, which meant that the amount of Bifidobacteria was increased more significantly on the affected side (P<0.05). For the comparison of D-value on Ct value of Lactobacilli between treatment before and after, there was no statistically significant difference between the affected side and healthy side (P>0.05). Conclusion:Ruyong Formula shows satisfactory efficacy in the treatment of lactational mastitis, which can significantly improve the clinical symptoms of patients. It may play the therapeutic role through regulating the mammary gland flora such as reducing pathogenic bacteria and increasing probiotics.关键词：lactational mastitis;Ruyong Formula;stagnated heat in liver and stomach;regulation of mammary gland flora;Staphylococcus;Bifidobacteria
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of Wentong Powder with Shenque acpoint application in the treatment of primary dysmenorrhea with syndrome of blood stasis due to coagulated cold, and discuss its possible mechanisms. Methods72 patients of primary dysmenorrhea with syndrome of blood stasis due to coagulated cold were randomly divided into the treatment group and control group, 36 cases in each group. The control group was treated with ibuprofen sustained release capsules and the treatment group was treated with acpoint application of Wentong Powder at Shenque point (CV8),with a course of 2 menstrual cycles. The Chinese medical syndrome score was compared, and the changes on the serum levels of cyclooxygenase-2 (COX-2), prostaglandin F2α (PGF2α), prostaglandin E2 (PGE2), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), estradiol (E2) and progesterone (P) were observed and compared. Results:①In both groups, there was statistically significant difference on Chinese medical syndrome score between treatment before and after (P<0.05). After treatment, the improvement on Chinese syndrome score in the treatment group was obviously better than that in the control group (P<0.05). ②Compared with treatment before, the levels of COX-2, PGF2α and PGF2α/PGE2 were significantly decreased in both groups after treatment (P<0.01), the level of PGE2 was significantly increased in the treatment group (P<0.01) but obviously decreased in the control group (P<0.01). After treatment, there were statistically significant differences on the levels of COX-2, PGF2α and PGE2 between the two groups (P<0.01), while there was no statistically significant difference on the level of PGF2α/PGE2 between the two groups (P＞0.05). ③Compared with treatment before, the levels of IL-6 and TNF-α were significantly decreased in both groups after treatment (P<0.01). After treatment, there were statistically significant differences on the levels of IL-6 and TNF-α between the two groups, and the levels in the treatment group were obviously lower than those in the control group (P<0.05). Compared with treatment before, the E2 level was significantly increased in the treatment group (P<0.01) but no obvious change was found in the control group (P>0.05) after treatment. There was no obvious change on the P level in both groups (P>0.05). After treatment, the E2 level in the treatment group was significantly higher than that in the control group (P<0.01). ④During the trial, no adverse reactions were observed in both groups, and no abnormality in routine examinations of blood, urine, stool and liver and kidney function was detected in both groups before and after treatment. Conclusion:Wentong Powder with Shenque acpoint application in the treatment of primary dysmenorrhea with syndrome of blood stasis due to coagulated cold can significantly improve the dysmenorrhea in patients. Its mechanisms may be related to increasing E2, decreasing COX-2, IL-6 and TNF-α and regulating PGs (decreasing PGF2α and increasing PGE2).关键词：primary dysmenorrhea;syndrome of blood stasis due to coagulated cold;Wentong Powder;Shenque point (CV8);umbilical therapy;nerve-endocrine-immunity network
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of auricular application therapy on the postoperative pain of tibia and fibula fracture. Methods66 patients with tibia and fibula fracture were randomly divided into the treatment group and control group, 33 cases in each group. Under the continuous epidural anesthesia, the patients in both groups were performed the internal fixation of intramedullary nailing for tibia fracture combined with open reduction and internal fixation for fibula fracture. After surgery, the premixed analgesic solution was injected by electric controlled analgesic injection pump. After operation, the control group was treated with basic nursing and the treatment group was treated with auricular application based on the basic nursing, with a course of 72 hours. The satisfaction of postoperative nursing was observed. The numerical rating scale (NRS) score, residual solution amount in analgesic pump and supplementary times of analgesic solution were compared. Results:①Compared with operation before, the NRS scores in both groups were obviously decreased at each timepoint after operation (P<0.05), which showed a tendency of gradually obvious reduction over time (P<0.05). There were statistically significant differences on NRS scores between the two groups at each timepoint after operation, and the scores in the treatment group were obviously lower than those in the control group (P<0.05). ②72 hours after operation, the residual solution amount in analgesic pump in the treatment group was obviously more than that in the control group (P<0.05), and the supplementary times of analgesic solution in the treatment group was obviously less than that in the control group (P<0.05). ③3 days after operation, the overall satisfactions of nursing were 90.0% in the treatment group and 69.7% in the control group, there was statistically significant difference on the satisfaction of nursing between the two groups (P<0.05). Conclusion:The auricular application therapy can obviously relieve the pain and discomfort in postoperative patients following tibia and fibula fracture, and improve the satisfaction of nursing.关键词：tibia and fibula fracture;postoperative pain;auricular application;analgesia;external treatment of traditional Chinese Medicine
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发布时间：2022-08-31 - 摘要：This paper summarizes the experience in differentiating and treating reflux esophagitis from balancing middle-jiao. The key pathogenesis of reflux esophagitis is imbalance of middle-jiao caused by spleen failing to ascend clear,failure of stomach qi to descend and adverse rising of stomach qi,which can present the syndromes of adverse rising of stomach qi and liver fire invading stomach. The treatments mainly focus on descending adverse qi for harmonizing stomach and balancing middle-jiao.关键词：reflux esophagitis;balancing middle-jiao;clinical experience
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发布时间：2022-08-31 - 摘要：This paper introduces the experience in the clinical differentiation and treatment of hypertension with traditional Chinese medicine. It is considered that,the antihypertensive therapy needs warming and nourishing,therapy of removing dampness and dissolving turbidity can be used for youth with hypertension,therapy of regulating and harmonizing yin and yang can be used for aged hypertensive patients,therapy of activating the blood circulation should not be ignored in the chronic hypertension. Based on the treatment according to syndrome differentiation,it also should pay attention to individualized treatment and comprehensive adjustment of physical condition.关键词：hypertension;therapy of traditional Chinese medicine;treatment according to syndrome differentiation
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发布时间：2022-08-31 - 关键词：glanzmann thrombasthenia;nourishing kidney water;experience of physician
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发布时间：2022-08-31 - 摘要：Objective:To explore the inhibiting effects and mechanisms of notoginsenoside R1 on liver lipid deposition in ApoE knocked out (ApoE-/-) mice. MethodsThe wild type C57BL/6J mice were taken as the normal group. The ApoE-/- mice were randomly divided into the model group and notoginsenoside R1 group. The normal group and the model group were treated with the solvent therapy, and the notoginsenoside R1 group was treated with notoginsenoside R1 at dose of 25 μg/g by intraperitoneal injection, with a course of 8 weeks. The changes on the liver pathological morphology and lipid deposition were observed after hematoxylin and eosin (HE) staining and oil red O staining. The total RNA was extracted from the liver. The gene expressions of PPARγ, LXRα, ABCA1, SRBI and the expressions of miR-26a, miR-33 and miR-122 were analyzed by Real-time PCR. Results:The results of HE staining and oil red O staining revealed that compared with the normal control group, large vacuolation and degenerative hepatocytes swelling were observed in the liver tissues of mice in the model group, as well as a great number of lipid vacuoles and lipid deposition. Compared with the model group, the hepatocytes swelling was significantly relieved and the lipid vacuoles and lipid deposition were reduced in the notoginsenoside R1 group. The results of expression analysis on lipid metabolism-related genes and miRNAs revealed that compared with the model group, the expression levels of PPARγ, LXRα, ABCA1 and SRBI were significantly up-regulated in the notoginsenoside R1 group (P＜0.05, P＜0.01), and the expression level of miR-26a was significantly down-regulated (P＜0.05). Conclusion:Notoginsenoside R1 can significantly suppress the liver lipid deposition in ApoE-/- mice, and its mechanisms may be associated with regulation on cholesterol metabolism.关键词：notoginsenoside R1;liver;cholesterol metabolism
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发布时间：2022-08-31 - 摘要：Objective:To observe the effects of Jingfang Baidu Powder on the protein and gene expressions of Nephrin, CD2-associated protein (CD2AP) and integrin linked kinase (ILK) in podocytes of adriamycin-induced nephropathy rat renal tissue. MethodsSD rats were randomly divided into the normal group, model group, benazepril group and Jingfang Baidu Powder group. Except the normal group, the rat model of nephropathy was established in other groups by single intravenous injection of adriamycin in tail. Each group was treated with the corresponding drug. The levels of 24 h urinary protein and renal function indexes in rats of each group were detected. The protein and gene expressions of Nephrin, CD2AP and ILK in the renal tissue were detected. Results:Compared with the normal group, the levels of 24 h urinary protein and renal function indexes were obviously increased in the model group, the protein and gene expression levels of Nephrin and CD2AP were obviously down-regulated, whereas the protein and gene expression levels of ILK were significantly up-regulated in the model group. Compared with the model group, the levels of 24 h urinary protein and renal function indexes were obviously decreased in the Jingfang Baidu Powder group, the protein and gene expression levels of Nephrin and CD2AP were obviously up-regulated, whereas the protein and gene expression levels of ILK were significantly down-regulated in the Jingfang Baidu Powder group. Conclusion:Jingfang Baidu Powder can decrease the proteinuria level in adriamycin-induced nephropathy rats. Its mechanism may be associated with the down-regulation of ILK expression and the up-regulation of Nephrin and CD2AP expressions in podocytes.关键词：Jingfang Baidu Powder;adriamycin-induced nephropathy;Nephrin;CD2-associated protein;ILK
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发布时间：2022-08-31 - 摘要：Objective:To investigate the effects of medicated serum of Liuwei Dihuang Pill on carboxylated and uncarboxylated osteocalcin in MC3T3-E1 cells, and further explore the common material basis of Liuwei Dihuang Pill in the treatment of osteoporosis and diabetes mellitus. MethodsThe suspension of Liuwei Dihuang Pill was prepared and given to the adult Wistar rats by intragastric administration, and then the medicated serum of Liuwei Dihuang Pill was collected. The cells were divided into the control group, medicated serum groups with low-, middle- and high-dose. The MC3T3-E1 cells were cultured for 22 days and starved for 24 hours, and then the culture medium was replaced by 10% medicated serum with different concentrations. After cultured for 24,8 and 72 hours respectively, 1 ml supernatant was aspirated from each group to detect carboxylated and uncarboxylated osteocalcin. The expression of osteocalcin mRNA was detected after cultured for 72 hours. Results:Compared with the control group, the expression of osteocalcin mRNA was significantly increased in the medicated serum groups with low-, middle- and high-dose (P<0.01). For uncarboxylated osteocalcin in the supernatant,the contents in the medicated serum groups with middle- and high-dose were significantly higher than those in the control group and low-dose group at each timepoint (P<0.01, P<0.05).For carboxylated osteocalcin in the supernatant, the content in the medicated serum group with low-dose was significantly higher than that in the control group after incubated for 48 hours (P<0.01), and the contents in the middle- and high-dose groups were significantly higher than those in the control group at each timepoint (P<0.01). Meanwhile, the content in the middle-dose group was significantly higher than that in the low-dose group after incubated for 24 and 72 hours (P<0.05), and the content in the high-dose group was significantly higher than that in the low-dose group (P<0.01) and the middle-dose group (P<0.05) after incubated for 72 hours. Conclusion:The medicated serum of Liuwei Dihuang Pill can promote the contents of carboxylated and uncarboxylated osteocalcin in the supernatant of MC3T3-E1 cells, which suggests that osteocalcin may be the basic material of Liuwei Dihuang Pill in treating two diseases, that is treating different diseases with the same method.关键词：medicated serum;osteocalcin;treating different diseases with the same method;Liuwei Dihuang Pill
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发布时间：2022-08-31 - 摘要：Objective:To discuss the effects of Bushen Huoxue Recipe on the differentiation and proliferation of mouse limb bud cells into chondrocytes. Methods The limb bud cells of 12.5-day mouse embryo were isolated and cultured, and then treated with medicated serum of Bushen Huoxue Recipe. The proliferation activity of limb bud cells was detected by MTT, the differentiation capacity of limb bud cells into chondrocytes was observed by alcian blue staining, and the mRNA expression levels of Col2a1, Col10a1, Mmp9, β-catenin and Runx2 in limb bud cells were detected by RT-PCR. Results:Compared with the blank control group, the proliferation of mouse limb bud cells was promoted in medicated serum group of Bushen Huoxue Recipe at timepoints of 24 h, 48 h and 72 h. After micromass culture for 6 days, the alcian blue staining showed that the cartilage formation was increased in the medicated serum group of Bushen Huoxue Recipe. The RT-PCR results showed that the mRNA expression level of Col2a1 in limb bud cells was up-regulated in the medicated serum group of Bushen Huoxue Recipe, while the mRNA expression levels of Col10a1, Mmp9, β-catenin and Runx2 were down-regulated. Conclusion:The medicated serum of Bushen Huoxue Recipe may promote the proliferation and differentiation of mouse embryo limb bud cells through β-catenin signaling pathway.关键词：limb bud cell;micromass culture;Bushen Huoxue Recipe;chondrogenesis
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发布时间：2022-08-31 - 摘要：Objective:To establish the optimum method of extracting chlorogenic acid from Jinyinhua (Lonicerae Japonicae Flos) by ionic liquid. MethodsThe conditions of extraction of chlorogenic acid from Jinyinhua (Lonicerae Japonicae Flos) by ionic liquid microwave and ionic liquid bath heating were investigated and optimized,respectively,and the comparison was made. Results:The optimum extraction method was as followed:the composition proportion in aqueous solution of ionic liquid was V / V water as 4/11,the ratio of liquid to material was 30,the extraction process was performed by 400 W microwave for 2.5 minutes. The average yield of chlorogenic acid was 3.238% under this processing condition. Conclusion:The method is fast,environmentally friendly and energy saving,with higher extraction rate.关键词：chlorogenic acid;ionic liquid;microwave method;water bath heating method
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发布时间：2022-08-31 - 摘要：This paper reviews the research progress on mechanism of commonly used Chinese herbal medicine in constipation. Studies have found that the laxative effects of Chinese herbal medicine are related to lubricating the intestinal tract,promoting the secretion of intestinal water and mucus,increasing the intestinal volume,enhancing the intestinal motility and regulating gastrointestinal hormones,neurotransmitters and Cajal interstitial cells.关键词：constipation;Chinese herbal medicine;mechanism;review
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发布时间：2022-08-31 - 摘要：This paper reviews the new research progress on the analytic determination methods of ginsenoside in recent years. There are many analytic determination methods of ginsenosides,mainly including colorimetry,high performance liquid chromatography,ultra high performance liquid chromatography,high performance liquid chromatography-tandem mass spectrometry,ultra high performance liquid chromatography-tandem mass spectrometry and micellar electrokinetic capillary chromatography. The specific application of the above methods in the determination of ginsenosides are introduced respectively, and on this basis,the summary and prospect are carried out.关键词：ginsenoside;determination methods;review
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发布时间：2022-08-31
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