最新刊期
期 3 ， 2018
- 摘要：This paper discusses Wang Xiafang’s experience in the differentiation and treatment of respiratory diseases in children. It is considered that the spleen and lung diseases are common in clinical pediatric diseases. It advocates the theory that stomach qi as root of all diseases,and the therapy of reinforcing earth for generating metal is widely applied in clinic,which form the academic thoughts for the treatment of respiratory diseases according to different periods and syndromes. It refers to treating lung in acute attack period and co-regulating spleen and lung in remission period and invigorating spleen in recovery period,which especially emphasizes the importance of invigorating spleen in recovery period.关键词：respiratory diseases;reinforcing earth for generating metal;experience of famous doctor;Wang Xiafang
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发布时间：2022-08-31 - 摘要：This paper tries to discuss the syndrome and treatment of plague by Wu Youke from specific diseases. Wu Youke’s treatment of plague focuses on the dredging and dispersing of qi movement. Sweating therapy should be applied according to the condition,and be sure to keep the gastrointestinal tract unobstructed. Dahuang(Rhei Radix et Rhizoma)is preferred to dredge and disperse,rather than Huanglian(Coptidis Rhizoma). It is considered that the bitter-flavour and cold-natured drugs could only treat the manifestations but not root cause. After sweating,the symptoms are relieved,the pathogen is removed and the vital qi is restored. The therapy of dredging promotes the pathogen into the surface and then the therapy of dispersing expels the pathogen out. Wenyi Lun(Treatise on Plague)was complemented and extended by Dai Tianzhang and Yang Lishan,and Wu Jutong also discussed the prescriptions,and Chongding Guang Wenre Lun was made by He Lianchen,which enlarged the syndrome and treatment of epidemic febrile disease.关键词：Wu Youke;Wenyi Lun(Treatise on Plague);plague;clinic;syndrome and treatment
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发布时间：2022-08-31 - 摘要：Analogy is one of the important philosophical thoughts of traditional Chinese medicine,and makes an important contribution to the formation and development of theory system of traditional Chinese medicine. With the development of modern life science,analogy in traditional Chinese medicine also has a new connotation. This paper attempts to use the modern life science terminology to elucidate the new understanding of analogy based on theory of yin-yang and concept of “fighting poison with poison”,and further explores the value of analogy of traditional Chinese medicine in the life science research and discovers the potential scientific logic,in order to guide and explore new scientific phenomena and solve key scientific issues in life sciences and medicine.关键词：traditional Chinese medicine;analogy;life science;theory of yin-yang;fighting poison with poison
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发布时间：2022-08-31 - 摘要：This paper mainly discusses the causes, mechanism, syndromes and treatments of allotriogeusia and abnormal tongue perception due to depression syndrome. Allotriogeusia includes bitter taste, sweet taste, sour taste, salty taste,pungent taste in the mouth. Abnormal tongue perception includes glossalgia, numb tongue, dry tongue, stiff tongue, swollen tongue and burning sensation of tongue. Allotriogeusia and abnormal tongue perception due to depression syndrome are caused by emotional factors. In addition to emotional manifestations, a series of complicated somatic symptoms can be observed simultaneously. The disease location mainly involves the liver-gallbladder, heart-kidney and spleen-stomach. And the pathogenesis and syndromes mainly involve depressed lesser yang, liver qi depression, liver fire, dampness-heat of liver-gallbladder, deficiency of heart-gallbladder, disturbance of heart spirit, heart fire, hyperactivity of fire due to yin deficiency, disharmony between heart and kidney and accumulated heat in spleen and stomach. As for the treatment, relieving depression according to different syndromes should be flexibly used. If necessary, it also should resolve phlegm and remove blood stasis. In modern medicine, psychological trauma, obsessive compulsive disorder, depression, anxiety disorder, hysteria, neurasthenia, and menopausal syndrome, burning mouth syndrome and other diseases related to emotional factors may result in allotriogeusia and abnormal tongue perception due to depression syndrome.关键词：allotriogeusia;abnormal tongue perception;etiology and pathogenesis;treatment based on relieving depression
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发布时间：2022-08-31 - 摘要：Objective:To discuss Professor Liu Fengbin’s medication rules in the treatment of ulcerative colitis based on traditional Chinese medicine(TCM)inheritance support system. MethodsProfessor Liu Fengbin’s prescriptions for ulcerative colitis were collected,the prescription characteristics and medication rules were analyzed by association rule mining and complex system entropy cluster in TCM inheritance support system. Results:①This study totally included 169 prescriptions involving 126 drugs with a cumulative frequency of 2 248. ②There were 34 kinds of drugs with frequency of usage more than 20 times. Among,12 drugs that with the highest frequency of usage(medication frequency more than 58 times as high frequency drugs)were Huoxiang(Agastaches Herba),Fuling(Poria),Huanglian(Coptidis Rhizoma),Taizishen(Pseudostellariae Radix),Muxiang(Aucklandiae Radix),Baizhu(Atractylodis Macrocephalae Rhizoma),Gancao(Glycyrrhizae Radix),Baihua Sheshecao(Hedyotis Herba),Huotanmu(Polygonum chinensis),Shanyao(Rhizoma Dioscoreae),Wumeitan(charred Mume Fructus),Qingdai(Naturalis Indigo). ③According to the number of prescriptions,the correlation (degree of association between drugs and drugs) was set as 8 and the penalty (parameters to reduce the interference of negative data information) was 2,then the cluster analysis was performed. 17 couplet medicines that correlation coefficient was more than 0.03 were obtained. ④Based on the complex system entropy clustering,under the constraints of correlation degree as 8 and penalty as 2,14 core combinations that contained 3-4 drugs were evolved. On this basis,by unsupervised entropy hierarchical clustering,seven new prescriptions were further combined and evolved. Conclusion:12 drugs with high frequency of usage,17 commonly used couplet medicines,14 core drug combinations and 7 new prescriptions are summarized. Professor Liu Fengbin has rich clinical experience in the treatment of ulcerative colitis. The products with effects of invigorating spleen for eliminating dampness and clearing heat and promoting diuresis are often used in the treatment,which aim at the pathogenesis of spleen deficiency as root cause and dampness heat as manifestation. TCM inheritance support system can be used for the study of experience of famous and veteran doctors of TCM.关键词：ulcerative colitis;prescriptions;medication rules;traditional Chinese medicine inheritance support system;Liu Fengbin
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发布时间：2022-08-31 - 摘要：This paper introduces Professor Wu Yaochi’s experience in treating multiple pain syndrome with qi-guiding acupuncture combined with substance-partitioned moxibustion,such as epigastric pain, headache, dysmenorrhea, back pain, facial pain, ophthalmodynia, pain of muscle and tendon injury, and arthromyodynia pain of neck, shoulder and knee. The clinical treatment should take acupuncture and moxibustion in combination,and emphasize the effective combination of acupoint massage and acupuncture.关键词：pain syndrome;qi-guiding acupuncture;substance-partitioned moxibustion
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发布时间：2022-08-31 - 摘要：This paper introduces Professor Jiang Zhiyan’s clinical experience in the treatment of adenoidal hypertrophy in children. It is considered that the etiology and pathogenesis of this disease are invasion of pathogenic toxin,lung-heat stagnated in nose,accumulation of phlegm dampness,phlegm obstruction due to spleen deficiency. The treatment is based on dissipating phlegm and resolving masses. When the disease presents acute onset and lung-heat stagnated in nose,therapy of clearing lung-heat for eliminating phlegm should be applied. When the disease presents chronic progress and phlegm obstruction due to spleen deficiency,it should strength the therapy of activating spleen-energy and opening orifice,with combination of oral administration of Chinese medicine and acupoint massage. Two proven cases are presented.关键词：adenoidal hypertrophy;treatment based on phlegm;clinical experience;Jiang Zhiyan
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发布时间：2022-08-31 - 摘要：This paper discusses the medication rule for treating diarrhea in Zhang Zhongjing’s Shanghan Lun(Treatise on Cold Pathogenic Diseases). The abnormal function of spleen and stomach is considered as the main cause of diarrhea. Drugs with sweet flavor,such as prepared Radix Glycyrrhizae as representitive,can be used in the treatment. And drugs of channel tropism in spleen and stomach are emphasized.关键词：diarrhea;medication rule;Shanghan Lun(Treatise on Cold Pathogenic Diseases)
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发布时间：2022-08-31 - 摘要：The related theory of constitution and disease in Shanghan Lun(Treatise on Cold Pathogenic Diseases) and Jingui Yaolve(Synopsis of Golden Chamber) are analyzed,including constitution and disease occurrence, constitution and disease tendency, constitution and disease transmission, constitution and disease treatment, constitution and disease prognosis. It is considered that disease occurrence is closely related to the normal state of promordial qi in five viscera,disease tendency is closely related to the age,sex and strength of vital qi；constitution differences can lead to different trends in transmission；in the disease treatment,especially the therapy of expelling pathogen such as diaphoresis and purgative method,different compatibility and dosage should be adopted according to the constitution differences；the key to determine the disease prognosis is the strength and weakness of constitution and the rise and fall of vital qi.关键词：Shanghan Lun(Treatise on Cold Pathogenic Diseases);Jingui Yaolve(Synopsis of Golden Chamber);constitution;disease occurrence;transmission;treatment;prognosis
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of Hezhong Jianpi Decoction in the treatment of gastroesophageal reflux disease(GERD). MethodsBy randomized, double-blind and controlled trial,92 GERD patients were randomly divided into the Chinese medicine group and control group,46 cases in each group. The control group was treated with omeprazole capsule and placebo of Hezhong Jianpi Decoction,and the Chinese medicine group was treated with Hezhong Jianpi Decoction and placebo of omeprazole,with a course of 8 weeks. The clinical efficacy was observed,and the changes on reflux disease questionnaire(RDQ),gastroesophageal reflux disease health-related quality of life(GERD-HRQL),Pittsburgh sleep quality index(PSQI)and hospital anxiety and depression scale(HADS) were compared. Results:①=1\*GB3Finally 85 cases completed the trial,among 43 cases in the Chinese medicine group and 42 cases in the control group. ②For the comparison of RDQ score between treatment before and after,there was statistically significant difference in both groups(P＜0.01). After treatment,there was statistically significant difference on RDQ score between the two groups(P＜0.05). ③The total effective rates were 88.37% in the Chinese medicine group and 80.95% in the control group. The clinical efficacy of the Chinese medicine group was better than that of the control group(P＜0.05). ④In both groups,there were statistically significant differences on scores of GERD-HRQL,PSQI and HADS between treatment before and after(P＜0.01). After treatment,there were statistically significant differences on scores of GERD-HRQL,PSQI and HADS between the two groups(P＜0.05). Conclusion:Hezhong Jianpi Decoction can significantly improve the clinical symptoms and mental state of patients with gastroesophageal reflux disease,and enhance the quality of life.关键词：gastroesophageal reflux disease;Hezhong Jianpi Decoction;randomized control;blindness;placebo
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发布时间：2022-08-31 - 摘要：Objective:To observe the effects of Danzhi Jiangtang Capsule on stromal cell-derived factor-1α(SDF-1α), Cystatin C(CysC)and renal vascular ultrasound in patients with early diabetic nephropathy. Methods90 patients with early diabetic nephropathy were randomly divided into the treatment group and control group, 45 cases in each group. The control group was treated with conventional hypoglycemic and antihypertensive drugs, the treatment group was treated with Danzhi Jiangtang Capsule based on the treatment for the control group,with a course of 3 months. The clinical efficacy was observed. The hemodynamic indexes in renal B-ultrasound and related biochemical indexes including fasting plasma glucose (FPG), postprandial 2 h plasma glucose (2 hPG), glycosylated hemoglobin (HbA1c), urinary microalbumin (mAlb), ratio of urinary albumin and creatinine (ACR) and serum creatinine (Scr) were compared, as well as SDF-1α and CysC. Results:①Finally 44 cases in the control group and 43 cases in the treatment group completed the trial. ②The clinical total effective rates of the treatment group and control group were 86.0% and 59.1% respectively. The clinical efficacy of the treatment group was significantly better than that of the control group (P<0.05). ③After treatment, there were no statistically significant differences on the levels of FPG, 2hPG and HbA1c between the two groups (P>0.05). ④After treatment, there were statistically significant differences on the levels of mAlb, ACR and Scr between the two groups, and the levels in the treatment group were significantly lower than those in the control group (P<0.01). ⑤After treatment, there were statistically significant differences on the levels of SDF-1α and CysC between the two groups, and the improvement in the treatment group was significantly better than that of the control group (P<0.05). ⑥After treatment, there was no statistically significant difference on Vmax between the two groups (P>0.05). There was statistically significant difference on RI between the two groups (P<0.05). Conclusion:Danzhi Jiangtang Capsule combined with routine western medicine shows good efficacy in the treatment of early diabetic nephropathy. It can reduce the serum CysC level, regulate the SDF-1α level, resist the inflammatory reaction, improve the renal perfusion and protect the renal function of patients.关键词：diabetic nephropathy;renal diabetes;Danzhi Jiangtang Capsule;inflammation repair;renal function protection;renal vascular lesions
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of Bushen Zhuanggu Decoction combined with conventional therapy in the treatment of restless legs syndrome in uremic patients with maintenance hemodialysis,and discuss its possible mechanism. Methods64 uremic maintenance hemodialysis patients with restless legs syndrome were randomly divided into the treatment group and control group, 32 cases in each group. The patients in both groups maintained the original stable diet habits,the hemodialysis treatment plans were improved and the basic diseases were treated. The control group was treated with calcitriol soft capsules and calcium carbonate tablets,the treatment group was treated with Bushen Zhuanggu Decoction based on the treatment for the control group,with a course of 6 months. The clinical efficacy was observed. The changes on the levels of blood calcium, blood phosphorus, ALP and iPTH and international restless legs syndrome study group (IRLSSG) questionnaire score (IRLSSG score) were compared. Results:①The clinical total effective rates of the treatment group and control group were 81.3% and 37.5%, respectively. The clinical efficacy of the treatment group was obviously better than that of the control group (P<0.05). ②After treatment, the improvement on the levels of blood calcium, blood phosphorus, ALP and iPTH and IRLSSG score in the treatment group was significantly better than that in the control group (P<0.05). Conclusion:Bushen Zhuanggu Decoction combined with conventional therapy shows good efficacy in the treatment of restless legs syndrome in uremic patients with maintenance hemodialysis. Its mechanism may be related to the improvement on disorders of bone metabolism and calcium and phosphorus metabolism.关键词：uremia;hemodialysis;restless legs syndrome;Bushen Zhuanggu Decoction;calcium and phosphorus metabolism;bone metabolism disorder
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of Shen’s Shengdi Qinlian Tufuling Decoction in the treatment of Behcet’s disease. Methods68 patients with Behcet’s disease were randomly divided into the treatment group and control group,34 cases in each group. The treatment group was treated with Shen’s Shengdi Qinlian Tufuling Decoction(for continuous 2 months)combined with thalidomide tablets(25 mg/day for one month and then withdraw),the control group was treated with thalidomide tablets(50 mg/day in the first month and 25 mg/day in the second month). The treatment course was 2 months in both group, and the patients were followed-up until 4 months after the end of treatment. The clinical efficacy and adverse reactions were observed, the changes on the effective rate and recurrence rate of main symptoms, immune and acute inflammation indexes were compared. Results:①The clinical total effective rates of the treatment group and control group were 94.1% and 85.3% respectively. The clinical efficacy of the treatment group was significantly better than that of the control group (P<0.05).②After treatment, there were statistically significant differences on the effective rates of mouth ulcer, ophthalmia and arthritis between the two groups (P <0.05), while there were no statistically significant differences on the effective rates of the other main symptoms (P> 0.05). At the end of follow-up,there were statistically significant differences on the recurrence rates of mouth ulcer, erythema nodosum and arthritis between the two groups, and the recurrence rates of the treatment group were significantly lower than those of the control group (P<0.05). ③In both groups,there were statistically significant differences on the levels of ESR and CRP between treatment before and after (P<0.05), while there were no statistically significant differences on the levels of IgG and C3 between treatment before and after (P> 0.05). After treatment,there were statistically significant differences on the levels of ESR and CRP between the two groups,and the levels in the treatment group were significantly lower than those in the control group (P<0.05). Conclusion:Shen’s Shengdi Qinlian Tufuling Decoction in the treatment of Behcet’s disease can significantly improve the clinical symptoms, reduce the recurrence rate and adverse reactions on the basis of reducing the dosage and time of thalidomide.关键词：Behcet’s disease;Behcet syndrome;Behcet’s syndrome;Shengdi Qinlian Tufuling Decoction;immunosuppression;enhancing efficacy and reducing toxicity
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发布时间：2022-08-31 - 摘要：Objective:To observe the effects of Bushen Huoxue Recipe on endometrial receptivity and clinical pregnancy rate of patients with thin endometrial ovulation disorder in clomiphene promoted ovulation cycle. Methods60 patients with thin endometrial ovulation disorder were randomly divided into the control group and treatment group, 30 cases in each group. Both groups were treated with clomiphene to promote ovulation. The control group was orally treated with estradiol valerate, the treatment group was treated with Bushen Huoxue Recipe based on the treatment for the control group. The treatment course was one menstrual cycle in both groups with follow-up after 3 menstrual cycles. The clinical pregnancy rate was observed. The changes on endometrial thickness and type, uterine attery pulsatility index (PI) and uterine attery resistance index (RI) on the day of ovulation were compared between the two groups. Results:①After treatment,the improvement on endometrial thickness and proportion of A type endometrium in the treatment group was significantly better than that in the control group(P＜0.05). ②After treatment,the decrease of PI and RI in the treatment group were greater than those in the control group(P＜0.05). ③After 3 menstrual cycles,the clinical pregnancy rates of the treatment group and the control group were 43.3% and 26.7% respectively. There was statistically significant difference on the clinical pregnancy rate between the two groups, and the clinical pregnancy rate of the treatment group was significantly higher than that of the control group(P＜0.05). Conclusion: In clomiphene promoted ovulation cycle, Bushen Huoxue Recipe combined with estradiol valerate can increase the endometrial receptivity of patients with thin endometrium, and thus improve the pregnancy rate.关键词：thin endometrium;ovulation disorder;tonifying kidney and activating blood;endometrial receptivity;clinical pregnancy
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of Chinese medicine with oral administration (Bushen Antai Recipe) and acupoint application (Yishen Baotai Plaster) in the treatment of early threatened abortion with syndrome of kidney deficiency. Methods86 cases patients of early threatened abortion with syndrome of kidney deficiency were randomly divided into the control group and treatment group, 43 cases in each group. The control group was treated with intramuscular injection of progesterone and acupoint application of Yishen Baotai Plaster, the treatment group was treated with Bushen Antai Recipe based on the treatment for the control group. The two groups were treated for 2 weeks with follow-up for 3 months. The clinical efficacy, short-term pregnancy prognosis and medication safety were observed, and the TCM syndrome score and the levels of serum sex hormone were compared, including progesterone (P), estradiol (E2) and human chorionic gonadotropin (HCG). Results:①The clinical total effective rates were 97.7% in the treatment group and 81.4% in the control group. The clinical efficacy of the treatment group was significantly better than the control group (P<0.05). ②After the treatment, there was statistically significant difference on the TCM syndrome score between the two groups, and the score of the treatment group was significantly lower than that of the control group (P<0.05). ③After treatment, there were statistically significant differences on the levels of P, E2 and HCG between the two groups, and the levels in the treatment group were significantly higher than those in the control group (P<0.05). ④The continued pregnancy rates were 97.7% in the treatment group and 83.7% in the control group,with statistically significant difference,and the continued pregnancy rate of the treatment group was significantly higher than that of the control group (P<0.05). ⑤There was no statistically significant difference on the incidence rate of adverse reactions between the two groups (4.7% vs 0%) (P>0.05). Conclusion:Therapy of Chinese medicine with oral administration (Bushen Antai Recipe) and acupoint application (Yishen Baotai Plaster) in the treatment of early threatened abortion with syndrome of kidney deficiency can relieve the clinical symptoms of patients, improve the serum levels of sex hormone and pregnancy prognosis, with good safety.关键词：early threatened abortion;kidney deficiency syndrome;combined internal and external treatment;sex hormone;pregnancy prognosis;tocolysis
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of Huoxue Bushen Mixture in the treatment of androgenic alopecia with syndrome of yin deficiency of liver and kidney,and study the effects of Huoxue Bushen Mixture on serum trace elements (Cu, Fe, Zn) in female patients with hair loss. Methods80 female patients of androgenic alopecia with syndrome of yin deficiency of liver and kidney were randomly divided into the treatment group and control group,40 cases in each group. The treatment group was treated with Huoxue Bushen Mixture, and the control group was treated with Huolisu Oral Liquid,with a course of 3 months. The clinical efficacy was observed,and the changes on the serum levels of trace elements (Cu, Fe, Zn) were compared. Results:①Finally 76 cases completed the trial,38 cases in each group. ②The total effective rates were 81.6% in the treatment group and 68.4% in the control group. The clinical efficacy of the treatment group was better than that of the control group(P<0.05). ③The improvement on hair loss and Chinese medical syndromes(soreness and weakness of waist and knees,insomnia due to vexation,irregular menstruation) in the treatment group was better than that in the control group(P<0.05). ④Compared with treatment before,the serum levels of Fe and Zn in both groups were increased after treatment(P<0.05),and the serum Fe level of the treatment group was higher than that of the control group(P<0.05). Conclusion:HuoXue Bushen Mixture can effectively reduce the hair loss in female patients of androgenic alopecia with syndrome of yin deficiency of liver and kidney,improve the Chinese medical syndromes and regulate the serum levels of trace elements关键词：HuoXue Bushen Mixture;androgenic alopecia;serum trace element
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发布时间：2022-08-31 - 摘要：This paper summarizes the ten efficacy features of Daizheshi(Haematitum)in clinic. Its pharmacological effects are made clear through analysis on its structural components in modern pharmacological studies. The processing methods of Daizheshi(Haematitum) are discussed,and its application in ophthalmology and otorhinolaryngology are summarized according to works of materia medica and viewpoints of physicians in past dynasties.关键词：Daizheshi(Haematitum);efficacy and indications;pharmacological research;processing technology;ophthalmology and otorhinolaryngology
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发布时间：2022-08-31 - 摘要：Objective:To discuss the mechanism of Gutongling Decoction in the treatment of pain due to bone metastasis based on the regulation of MAPK signaling pathway. MethodsThe mouse model of bone metastasis was established,and 40 mice were randomly divided into the sham operation group, model group, Chinese medicine group and control group. Except the sham operation group, the mouse model of bone metastasis was established in the other groups. From 7th day of modeling, the Chinese medicine group was treated with Gutongling Decoction and the control group was treated with tramadol. The mechanical pain threshold was detected by VonFrey fiber to evaluate the analgesic efficacy. The pathology of liver, kidney and heart were observed to evaluate the drug safety. The protein expressions of p-JNK, p-p38 and p-ERK in MAPK signaling pathway in spinal cord were detected by Western blot. Results:①On the 14th day after modeling, compared with the model group, the mechanical pain threshold was increased in both control group and Chinese medicine group (P<0.05). On the 21st day after modeling, compared with the model group and control group, the mechanical pain threshold was increased in the Chinese medicine group (P<0.05). ②There were no significant changes on the pathology of liver, kidney and heart. ③Compared with the sham operation group, the expressions of p-JNK, p-p38 and p-ERK in spinal cord were increased in the model group (P<0.05). Compared with the model group, the expressions of p-JNK, p-p38 and p-ERK were significantly decreased in the control group and Chinese medicine group (P<0.05). There were no significant differences on the expressions of p-JNK, p-p38 and p-ERK between the Chinese medicine group and control group (P>0.05). Conclusion:Gutongling Decoction shows good efficacy in the treatment of pain due to bone metastasis, with no obvious adverse effects on heart, liver and kidney. Its analgesic mechanism is related to regulating the protein expressions of p-JNK, p-p38, p-ERK in MAPK signaling pathway.关键词：bone metastasis;pain;Gutongling Decoction;MARK signaling pathway
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发布时间：2022-08-31 -
Synergistic effects of Chaihu (Bupleuri Radix) on TIMP-1 and TGF-β1 in rats with pancreatic fibrosis
摘要：Objective:To discuss the synergistic effects and mechanism of channel ushering drug Chaihu (Bupleuri Radix) on tissue inhibitor of metalloproteinases-1 (TIMP-1) and transforming growth factor-β1 (TGF-β1) in rats with pancreatic fibrosis. Methods50 SD rats were randomly divided into the model group, sham operation group, Chaihu group, Dahuang (Rhei Radix et Rhizoma)-Danshen (Salviae Miltiorrhizae Radix et Rhizoma) group and Chaihu-Dahuang-Danshen group. In sham operation group, the rats were performed laparotomy and then the abdominal cavities was closed without any operation, while in the model group and three drug treatment groups, the rat model of pancreatic fibrosis was established by pancreatic duct ligation combined with cerulein by intraperitoneal injection. Before and after operation, three drug treatment groups were treated with Chaihu, Dahuang-Danshen and Chaihu-Dahuang-Danshen by intragastric administration at doses of 1.38g·kg-1d-1, 3.04 g·kg-1d-1 and 4.11 g·kg-1d-1, respectively, with a course of 3 days. Rats were sacrificed and the samples were collected on the 4th day after operation. The expression of serum TIMP-1 was measured by ELISA and the expression of TGF-β1 in pancreas was measured by immunohistochemistry. The mRNA and protein expression levels of TGF-β1 in pancreas were detected by Real-time PCR and Western blot, respectively. Results:Compared with the model group, the level of serum TIMP-1 was decreased in three drug treatment groups (P<0.01). Compared with Chaihu group, the level of TIMP-1 was decreased in Chaihu-Danhuang-Dansheng group (P<0.01). Compared with Dahuang-Danshen group, the level of TIMP-1 was decreased in Chaihu-Danhuang-Dansheng group (P<0.01). Compared with the model group, the mRNA and protein expressions of TGF-β1 were decreased in three drug treatment groups (P<0.01). Compared with Chaihu group, the mRNA and protein expressions of TGF-β1 were decreased in Chaihu-Dahuang-Danshen group (P<0.01), and the protein expression of TGF-β1 was decreased in Dahuang-Danshen group (P<0.01). The protein expression of TGF-β1 was decreased in Chaihu-Dahuang-Danshen group compared with Dahuang-Danshen group (P<0.01). Conclusion:“Down-regulating TGF-β1 expression- inhibiting PSC activation-reducing ECM synthesis” and “down-regulating TGF-β1 expression- inhibiting TIMP secretion-promoting ECM degradation” may be two main lines in anti-pancreatic fibrosis effects of Chaihu, Dahuang-Danshen and Chaihu-Dahuang-Danshen. The regulation effects of these three drugs on TGF-β1 play a dual role in ECM synthesis and degradation. Chaihu shows some synergism on the anti-pancreatic fibrosis effects of Dahuang-Danshen, which mainly displays in inhibiting TIMP-1 secretion and down-regulating protein expression of TGF-β1.关键词：pancreatic fibrosis;ECM synthesis and degradation;channel ushering drug;synergistic effects;TGF-β1;TIMP-1- 5
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发布时间：2022-08-31 - 摘要：Objective:To observe the effects of Jiedu Huazhuo Decoction containing serum on the related proteins expression of PI3K/Akt signaling pathway in HER-2-positive breast cancer, and discuss the mechanism of Jiedu Huazhuo Decoction in the treatment of HER-2-positive breast cancer. MethodsFemale Wistar rats were randomly divided into the control group, Chinese medicine group, western medicine group and combination group. The drug containing serum was prepared from the rats treated with the corresponding drugs, and then the SK-BR-3 cells were treated with different drug containing serum. The protein expressions of HER-2, p-HER-2 and PI3K/Akt pathway-related proteins p-Akt, Bad and p-Bad were detected by Western blot. Results:Western blot analysis showed that the grayscales of HER-2 and p-HER-2 in Chinese medicine group, western medicine group and combination group were obviously lower than those in the control group. The grayscales of HER-2, p-HER-2 and p-Akt in western medicine group and combination group were obviously decreased compared with the control group, and there were statistically significant differences on the expression levels of HER-2, p-HER-2 and p-Akt between the control group and western medicine group or combination group (P<0.05). The grayscales of Bad and p-Bad in Chinese medicine group, western medicine group and combination group were lower than those in the control group. There was no statistically significant difference on the total Bad expression among the groups (P>0.05). There was statistically significant difference on the p-Bad expression between the control group and western medicine group or combination group (P<0.05). Conclusion:Jiedu Huazhuo Decoction can down-regulate the expression of related proteins in PI3K/Akt signaling pathway and exert anti-tumor effects combined with inhibitors through synergy and attenuation.关键词：Jiedu Huazhuo Decoction;HER-2 positive;PI3K/Akt;breast cancer
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发布时间：2022-08-31 - 摘要：Objective:To observe the expressions of NALP3 inflammasome and IL-1β in rats with acute gouty arthritis, and discuss the effects and possible mechanism of fire needle on acute gouty arthritis. Methods75 Wistar rats were randomly divided into the normal group, model group, fire needle group, acupuncture group and colchicine group, 15 rats in each group. Except the normal group, the rat model was established by oteracil potassium combined with Coderre in the other groups. Each group was treated with the corresponding intervention. The samples were collected and recorded on the 1st, 4th and 7th day of treatment. The serum levels of NALP3 and IL-1β were detected by enzyme linked immunosorbent assays. The levels of NALP3 and IL-1β in synovium of joint were detected by immunohistochemistry. Results:①On the 1st day of treatment, compared with the normal group, the levels of NALP3 inflammasome and IL-1β in synovium of joint in the model group, fire needle group, acupuncture group and colchicine group were increased, with statistically significant differences (P<0.05).②On the 4th day of treatment, compared with the normal group, the serum levels of NALP3 inflammasome in the model group and acupuncture group were significantly increased, with statistically significant differences (P<0.01); and the serum IL-1β level was increased in the model group and acupuncture group, with statistically significant differences (P<0.01). Compared with the model group, the serum expressions of IL-1β and NALP3 in the fire needle group and colchicine group were inhibited, with statistically significant differences (P<0.01). Compared with the model group, the expressions of IL-1β and NALP3 in synovium of joint in the fire needle group, acupuncture group and colchicine group were inhibited, with statistically significant differences (P<0.05).③On the 7th day of treatment, compared with the normal group, the serum IL-1β level in the model group was still at a high level, with statistically significant difference (P<0.05). Compared with the model group, the serum IL-1β level was obviously decreased in the colchicine group with statistically significant difference (P<0.05). The level of NALP3 inflammasome in all groups returned to normal level and no statistically significant differences were observed among groups (P>0.05). Conclusion:The molecular mechanisms of fire needle in the treatment of acute gouty arthritis may be related to the inhibiting effects of fire needle on the activation of serum NALP3 inflammasome and the maturation and secretion of IL-1β.关键词：hyperuricemia;acute gouty arthritis;fire needle;NALP3 inflammasome;IL-1β
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发布时间：2022-08-31 - 摘要：Objective:To establish the quality control methods for Xiakucao (Prunellae Spica)-Wubeizi (Acanthopanacis Cortex) bacteriostasis lotion, and study its bacteriostatic effects on common gynecological bacteria in vitro. MethodsThe content of gallic acid, the main active component of the lotion, was detected by high performance liquid chromatography (HPLC). The separation was performed on an Alltima C18 column (4.6 mm*250 mm, 5 μm) by gradient elution with the mobile phases of 0.1% phosphoric acid dissolved methanol-0.1% phosphoric acid (21∶79) at a flow rate of 1.0 ml/min. The temperature of the column was 30 ℃ and the injection volume was 10 μl. The detection wavelength was 273 nm. The bacteriostatic effect of the lotion was verified by Kirby-Bauer susceptibility disk tests and bacteriostasis test. Results:The linear curve of gallic acid fitted well in the range of 0.015 g/L-0.245 g/L (r=1). The average recovery was 95.46%. Bacteriostasis tests in vitro showed good bacteriostatic activity of Xiakucao-Wubeizi lotion against Staphylococcus aureus and Escherichia coli. Conclusion:Determination of gallic acid in the lotion by HPLC is reliable for quality control. Bacteriostasis tests on common gynecological bacteria in vitro indicate that Xiakucao-Wubeizi lotion is an effective pudenda bacteriostasis agent.关键词：gallic acid;quality control;HPLC;bacteriostasis
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发布时间：2022-08-31 - 摘要：This paper reviews the research progress on antitumor mechanism of curcumin. Curcumin shows a certain efficacy on many kinds of cancer, such as colon, breast and pancreas cancer. At present, many molecular biological mechanisms are considered to be associated with the antitumor effects of curcumin,including regulating the unlimited proliferation of cancer cells and inducing apoptosis, regulating various signal pathways, inhibiting tumor angiogenesis and inhibiting metastasis.关键词：curcumin;tumor;mechanism;review
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发布时间：2022-08-31 - 摘要：The paper reviews the evaluation methods of chronic pain in rats in recent years. Based on the behavioral responses of rats,the specific operation,application and inadequacies of the evaluation methods,including withdraw claw reflex,tail-flick reflex,spontaneous pain behavior test,hot-plate test,abdominal withdraw reflex and facial expressions scale of rats,are briefly described and analyzed,and the brief prediction about the evaluation methods of chronic pain in rats in the future are made.关键词：chronic pain;behavior;evaluation methods;review
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发布时间：2022-08-31
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