最新刊期
期 11 ， 2017
- 摘要：This paper introduces Professor Shi Qi’s experience in the treatment of cervical spondylopathy with traditional Chinese medicine. He proposes the combination of disease differentiation, syndrome differentiation and pattern differentiation. According to the different patterns including cervical type,radiculopathy,vertebral arteriopathy,myelopathy and sympathetic type, based on the theories of traditional Chinese medicine,combined with years of clinical experience,he has formed a series of empirical formula with significant efficacy.关键词：cervical spondylopathy;therapy of traditional Chinese medicine;experience of famous doctor;Shi Qi
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发布时间：2022-08-31 - 摘要：Anorexia is not only a common symptom of spleen and stomach diseases,but also may be one of the manifestations of depression syndrome. Anorexia due to depression syndrome refers to anorexia triggered or aggravated by emotional factors and treated effectively by alleviating depression. There are two patterns of anorexia due to depression syndrome,one is single depression syndrome,which involves several pathogenesis such as stagnation of liver qi,stagnant liver qi transforming into fire and stagnation of heart-spleen；the other is coexistence of diseases and depression syndrome,no matter diseases induced by depression syndrome or depression syndrome induced by diseases,the main pathogenesis is incoordination between liver and stomach. Some mental disorders such as depression,dysthymia,anorexia nervosa,somatization disorder, and functional gastrointestinal disorders such as functional dyspepsia caused by psychological factors and mainly manifests as loss of appetite,can be diagnosed and treated according to “anorexia due to depression syndrome”.关键词：depression syndrome;anorexia;etiology and pathogenesis;therapeutic methods and prescriptions
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发布时间：2022-08-31 - 摘要：Objective:Based on the proven cases of primary immune thrombocytopenia that treated by Professor Huang zhenqiao,a national famous veteran practioner of traditional Chinese medicine,this study aimed to analyze the medication rules by data mining. Methods119 medical cases(involved 357 times visit)with definite diagnosis and relatively complete records were selected. After pretreatment,the standardized medical record database was established. Through software such as Microsoft Office Excel 2003,SPSS 18.0 and IBM SPSS Modeler 14.1,data mining were performed by frequency analysis,system cluster analysis and association analysis Apriori algorithm,respectively. From some aspects including commonly used Chinese materia medica,efficacy classification of Chinese materia medica,core drug groups,commonly used couplet medicines and drug and symptom correlation,Professor Huang Zhenqiao’s medication rules and characteristics in the treatment of primary immune thrombocytopenia were discussed. Results:①A total of 217 kinds of Chinese materia medica were used. The commonly used drugs included Radix Astragali seu Hedysari,Radix Rehmanniae Recens,Rhizoma Atractylodis Macrocephalae,Radix Glycyrrhizae Preparata,Cortex Moutan Radicis,Radix Scutellariae,Radix Paeoniae Alba,Herba Taraxaci,Radix Rehmanniae Preparata,Poria,Pericarpium Citri Reticulatae,Radix Glycyrrhizae,Radix Codonopsis,Eclipta alba,Cortex Phellodendri,Cornu Bubali,Radix Angelicae Sinensis,Radix Rubiae,Radix Paeoniae Rubra,Radix Pseudostellariae,Rhizoma Coptidis,Caulis Perillae and Rhizoma Dioscoreae. The tonifying deficiency medicine,clearing heat medicine and blood-stanching medicine were commonly used Chinese materia medica. ②By systematic cluster analysis,six core drug groups were found. The 1st group included Radix Rehmanniae Recens,Cortex Moutan Radicis,Radix Scutellariae,Radix Paeoniae Alba,Radix Rehmanniae Preparata,Cortex Phellodendri and Cornu Bubali；the 2nd group included Rhizoma Atractylodis Macrocephalae,Radix Astragali seu Hedysari and Radix Glycyrrhizae Preparata；the 3rd group included Herba Taraxaci,Poria,Pericarpium Citri Reticulatae and Radix Glycyrrhizae；the 4th group included Radix Codonopsis and Radix Angelicae Sinensis；the 5th group included Eclipta alba,Radix Pseudostellariae and Caulis Perillae；the 6th group included Radix Rubiae,Radix Paeoniae Rubra,Rhizoma Coptidis and Rhizoma Dioscoreae. ③The association rule analysis showed that Professor Huang emphasized the compatibility of medicines in clinical practice,including yang-supplementing medicine with qi-supplementing,blood-supplementing,diuresis and diffusing dampness drugs,clearing heat and resolving phlegm medicine with relieving cough and asthma drugs and clearing heat and removing toxicity drugs,medicine for tranquillization with heavy prescription and suppressing hyperactive liver yang medicine with yin-supplementing drugs,qi-supplementing drugs and clearing heat and removing dampness drugs. Meanwhile some correlation rules of drug and symptom were summarized. Conclusion:Professor Huang considers that the basic principles in the treatment of primary immune thrombocytopenia are purging fire to treat manifestation and suppressing liver-wood,treating both spleen and kidney to regulate yin and yang. The main treatment methods include clearing heat and cooling blood,benefiting qi for strengthening spleen,benefiting qi for nourishing blood,invigorating spleen for benefiting kidney,cooling blood for removing blood stasis and so on,and 6 kinds of commonly used drugs are summarized. Data mining can be used in the research on experience of famous veteran practioner of traditional Chinese medicine.关键词：primary immune thrombocytopenia;purpura;experience of famous doctors;Huang Zhenqiao;data mining
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发布时间：2022-08-31 - 摘要：This paper introduces Professor Li Xiangyun’s experience in the differentiation and treatment of polycystic ovarian syndrome(PCOS)with phlegm-dampness syndrome. He considers that the main pathogenesis are dysfunction of spleen in transportation and abnormal metabolism of body fluid that cause the dampness accumulation and phlegm generation,blockage in Chong and Ren Channels and uterus,which present phlegm-dampness as the manifestations and spleen deficiency as the root cause. Drugs for invigorating spleen and drying dampness can be used and combined with qi regulated medicines to enhance the effects of removing phlegm. Modified Cangfu Daotan Decoction is the recommend prescription. The commonly used couplet medicines for dissipating phlegm and eliminating dampness include Rhizoma Acori Tatarinowii and Lapis Chloriti,Herba Lycopi and Rhizoma Alismatis,Rhizoma Atractylodis Macrocephalae and Rhizoma Atractylodis,Herba Agastaches and Herba Eupatorii,Rhizoma Pinelliae and Caulis Bambusae in Taenia,Radix Aconiti Lateralis Preparata and Ramulus Cinnamomi. According to syndrome differentiation,the couplet medicines are selected or separated and used in combination,and properly regulated combined with menstrual cycle.关键词：polycystic ovary syndrome with phlegm-dampness syndrome;insulin resistance;dissipating phlegm and eliminating dampness
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发布时间：2022-08-31 - 摘要：This paper introduces Professor Zhu Shudong’s experience in the treatment of halitosis. He considers that“stagnation”is the key pathogenesis of halitosis. And according to the theory that“spleen in the smell is incense”,the aromatics can be used in the treatment to resolve dampness and turbid and dispel stagnated fire.关键词：halitosis;aromatics;clinical experience;Zhu Shudong
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发布时间：2022-08-31 - 摘要：This paper summarizes Gong Yuping’s experience in the treatment of ulcerative colitis and the medication characteristics. She considers that the pathogenesis are deficiency of spleen and stomach as the root cause and dampness,heat and pathogenic toxin as the manifestations,which combined with static blood blocking collaterals throughout. So the basic therapies are invigorating spleen and clearing intestine and promoting blood circulation for removing blood stasis. Jianpi Qingchang Recipe can be used in the treatment,and the internal and external treatment can be applied in combination. One proven case is presented.关键词：ulcerative colitis;hematochizia;turbid toxin;experience of famous doctors;Gong Yuping
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发布时间：2022-08-31 - 摘要：The concept of “Qizheng”was first proposed in Lao Zi,and used to classify and review the art of war in Military Science of Sun Zi(Sunzi Bingfa). Under its influence,traditional Chinese medicine also use the concept of “Qizheng”for the classification of things. According to the basic spirit of “Qizheng”concept,from the view of ideological principle,the Chinese medical treatment methods can be divided into two major categories. One is taking the opposite treatments and performing the direct confrontation for the pathogenic factors and disease nature,such as treating cold syndrome with hot natured drugs,treating heat syndrome with cold natured drugs,treating deficiency syndrome with tonifying method,treating excess syndrome with purgative method and so on. This comes from the usual principle(“Zheng”)in art of war,which is consistent with conventional transaction processing,and can be called as“orthodox treatment”. The other is not direct confrontation,but taking treatments according to the pathogen,location,condition and other characteristics of diseases or based on the whole body connection,such as adroitly to guide one’s actions according to circumstances,fire stagnation requiring dissipation,treating yin for the yang disease and so on. This method comes from the unusual principle(“Qi”)in art of war,which is somewhat different from conventional transaction processing,and can be called as“retrograde treatment”. A thorough understanding of the ideological principles of various Chinese medical treatment methods is of great significance for the improvement of therapeutic system of traditional Chinese medicine and correct application of these treatment methods.关键词：“Qizheng”;art of war;treatment methods
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发布时间：2022-08-31 - 摘要：By studying the ancient medical cases in past dynasties,treatment on thirst from middle energizer often based on the pathogenesis of exuberance of stomach fire and deficiency of stomach yin,but there were few cases that involved the syndrome and treatment of thirst due to deficiency of spleen yin. Based on the deep discussion on the pathogenesis of thirst,combined with the physiological characteristics of spleen,it put forward that the main pathogenesis of thirst due to deficiency of spleen yin were dysfunction of spleen in ascending clear,hyperactivity of fire due to yin deficiency and deficiency of both qi and yin,and the characteristics of disease and the key points of differentiation were expounded in detail,and the main therapy was nourishing spleen yin and promoting fluid production for moistening tongue,which provided new ideas and methods for the clinical diagnosis and treatment of thirst.关键词：thirsty;deficiency of spleen yin;nourishing spleen yin;syndrome differentiation and treatment
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发布时间：2022-08-31 - 摘要：This paper discussed Ma Peizhi’s academic thoughts and characteristics in the clinical treatment of gynecological diseases. By studying the medical cases,diagnosis methods,prescriptions and drugs that associated with gynecological diseases in Medical Records of Four Doctors in Menghe(Menghe Sijia Yiji),from etiology and pathogenesis,treatment methods,medication characteristics and other aspects,combined with various medical records,Ma Peizhi’s experience in the diagnosis and treatment of gynecological diseases were summarized. In Ma’s treatment on the menstrual disease,it emphasized the liver and spleen,and proposed the internal and external treatment for morbid vaginal discharge in combination. Before pregnancy,it should pay attention to the antenatal training and antenatal care,and prevent the illness of mother viscera affecting the child one；and the postpartum diseases should focus on deficiency and stasis.关键词：gynecological diseases;characteristic of diagnosis and treatment;Ma Peizhi
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发布时间：2022-08-31 - 关键词：hepatitis B cirrhosis;esophageal and gastric varices;clinical experience;Liu Ping
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of Modified Wuwei Xiaodu Decoction in the prevention and treatment of radiation pneumonia(RP)in patients with non-small cell lung cancer after chemotherapy. Methods120 patients with non-small cell lung cancer were randomly divided into the treatment group and control group,60 cases in each group. The control group was treated with conventional radiotherapy and chemotherapy,and the treatment group was treated with Modified Wuwei Xiaodu Decoction based on the treatment for the control group. The treatment course of radiotherapy and Chinese medicine were both one month. The incidence of RP and disease condition grades in both groups were observed,and the changes on Karnofsky performance status(KPS)score and serum transforming growth factor-β1(TGF-β1)that related to lung injury were compared. Results:①A total of 110 cases completed the trial,among 55 cases in the treatment group and 55 cases in the control group(5 cases in each group fell off). The total expulsion rate was 8.3%. ②During the trial,the incidence rates of RP were 5.45% in the treatment group and 20.00% in the control group,the incidence rate of the treatment group was obviously lower than that of the control group(P<0.05). There was statistically significant difference on the disease condition grades between the two groups(P<0.05). ③After treatment,there was statistically significant difference on the KPS score between the two groups,and the score of the treatment group was obviously higher than that of the control group(P<0.05). ④After treatment,there was statistically significant difference on the TGF-β1 level between the two groups,and the level of the treatment group was obviously lower than that of the control group(P<0.05). Conclusion:Modified Wuwei Xiaodu Decoction can be used in the prevention and treatment of radiation pneumonia in patients with non-small cell lung cancer after chemotherapy,which can obviously reduce the incidence of RP,ease the degree of acute radiation lung injury and improve the patient’s physical status.关键词：Modified Wuwei Xiaodu Decoction;non-small cell lung cancer;radiation pneumonia;preventive treatment of disease;clearing heat and toxicity;benefiting qi and nourishing yin
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of Gualou Xiebai Granule in the prevention and treatment of percutaneous coronary intervention(PCI)postoperative restenosis in soft plaque of coronary artery with syndrome of phlegm-dampness blocking. Methods100 patients with soft plaque of coronary artery of phlegm-dampness blocking syndrome who had received PCI operation were randomly divided into the treatment group and control group,50 cases in each group. The control group was treated with the routine therapy of western medicine,and the treatment group was treated with Gualou Xiebai Granule based on the treatment for the control group,with a course of one years. The PCI postoperative restenosis of coronary artery was observed,and the blood lipid metabolism related indexes(including total cholesterol ,triglyceride ,low density lipoprotein and high density lipoprotein )and Chinese medical syndrome score were compared before and after treatment. Results:①During the trial,5 cases in the treatment group and 5 cases in the control group fell off,and finally 90 cases completed the trial. ②The incidence rates of PCI postoperative restenosis of coronary artery were 2.22% in the treatment group and 20.00% in the control group. There was statistically significant difference on the incidence rate of PCI postoperative restenosis of coronary artery between the two groups,and the incidence rate of the treatment group was obviously lower than that of the control group(P<0.05). ③After treatment,there were statistically significant differences on the levels of TC,TG,LDL-C and HDL-C between the two groups,and the levels of TC,TG and LDL-C in the treatment group were obviously lower than those in the control group,the HDL-C level in the treatment group was obviously higher than that in the control group(P<0.05). ④After treatment,the Chinese medical syndrome score of the treatment group was obviously lower than that of the control group(P<0.05). Conclusion:Gualou Xiebai Granule combined with conventional western medicine can obviously decrease the incidence rate of PCI postoperative restenosis in soft plaque of coronary artery with syndrome of phlegm-dampness blocking,reduce the levels of blood lipid and improve the clinical symptoms.关键词：coronary heart disease;percutaneous coronary intervention;soft plaque;Fructus Trichosanthis;Bulbus Allii Macrostemonis;intravascular ultrasound;dissipating phlegm and resolving masses
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发布时间：2022-08-31 - 摘要：Objective:To observe the influence of comprehensive non-drug interventions of traditional Chinese medicine in community pre-diabetes population. Methods120 pre-diabetic patients were randomly divided into the treatment group and control group,60 cases in each group. The treatment group was treated with comprehensive non-drug interventions of traditional Chinese medicine(Chinese medical kinesiotherapy and auricular point pressing)combined with health education and management,and the control group was treated with conventional interventions(health education and management),with a course of one year. The changes on the glucose tolerance outcomes,related indicators of glucose metabolism and cardiovascular risk factors(carotid intima-media thickness,serum creatinine and urinary microalbumin excretion rate)were observed and compared. Results:①During the treatment,the incidence rates of diabetes were 0.0% in the treatment group and 5.0% in the control group,with statistically significant difference(P<0.05).The normalization rates of glucose tolerance were 48.3% in the treatment group and 11.7% in the control group,with statistically significant difference(P<0.05). ②After treatment,there were statistically significant differences on the levels of fasting blood glucose,postprandial 2 h blood glucose and glycosylated hemoglobin between the two groups,and the levels in the treatment group were obviously lower than those in the control group(P<0.05). ③After treatment,there were statistically significant differences on the levels of carotid intima-media thickness,serum creatinine and urinary microalbumin excretion rate between the two groups,and the improvement in the treatment group were obviously better than those in the control group(P<0.05). Conclusion:The comprehensive non-drug interventions of traditional Chinese medicine combined with health education and management can obviously improve the levels of glucose metabolism in community pre-diabetic patients,decrease the cardiovascular risk factors,and thus reduce the incidence rate of diabetes to a certain extent.关键词：pre-diabetes;preventive treatment of disease;non-drug therapy;Chinese medical exercise;auricular point pressing;community population
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of Chaihu Liuwei Decoction in the treatment of migrainous vertigo. Methods68 patients with migrainous vertigo were randomly divided into the control group and treatment group,34 cases in each group. The control group was treated with flunarizine hydrochloride capsules,and the treatment group was treated with flunarizine hydrochloride capsules and Chaihu Liuwei Decoction. The changes on Chinese medical scores,scores of quality of life and headache index score were observed in both groups. Results:①After treatment,the Chinese medical scores were decreased in both groups compared with treatment before(P<0.05),and the scores of the treatment group were lower than those of the control group(P<0.05). ②After treatment,the scores of quality of life were obviously increased in both groups(P<0.05),and the scores of the treatment group were higher than those of the control group(P<0.05). ③After treatment,the headache index score was obviously decreased in both groups(P<0.05),and the score of the treatment group was lower than that of the control group(P<0.05). Conclusion:Chaihu Liuwei Decoction can obviously improve the headache and other symptoms in the treatment of migrainous vertigo,and enhance the life quality of patients.关键词：migrainous vertigo;Chaihu Liuwei Decoction;quality of life;headache index
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of Pingle therapeutic manipulation for injured soft tissue combined with fumigation and steaming therapy of Chinese medicine in the treatment of knee osteoarthritis. Methods120 patients with knee osteoarthritis were randomly divided into the treatment group and control group,60 cases in each group. Both groups performed the autonomic function exercise of knee joint. The treatment group was treated with Pingle therapeutic manipulation for injured soft tissue combined with fumigation and steaming therapy of Chinese medicine,and the control group was treated with acupoint pressure on knee joint combined with dog skin plaster,with a course of one month. The clinical efficacy was observed,and the changes on the Lequesne indexes and visual analogue scale(VAS)score were compared. Results:①During the trial,2 cases in the treatment group and 3 cases in the control group fell off,and finally 115 cases completed the trial. ②The total clinical effective rates were 96.55% in the treatment group and 84.21% in the control group. The clinical efficacy of the treatment group was obviously better than that of the control group(P<0.05). ③After treatment,there were statistically significant differences on the joint pain,maximum walking distance,daily living ability index and comprehensive index and VAS score between the two groups,and the levels in the treatment group were obviously lower than those in the control group(P<0.05). Conclusion:Pingle therapeutic manipulation for injured soft tissue combined with fumigation and steaming therapy of Chinese medicine can be used in the treatment of knee osteoarthritis,which can obviously improve the function of knee joint,relieve the pain and enhance the life quality of patients.关键词：knee osteoarthritis;Pingle bone-setting;therapeutic manipulation for injured soft tissue;fumigation and steaming therapy of Chinese medicine;joint pain
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发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of Zhu’s Qingre Huayu Recipe in the treatment of pelvic related pain due to endometriosis with syndrome of intermingled blood stasis and heat. Methods120 endometriosis patients of intermingled blood stasis and heat syndrome with pelvic related pain were randomly divided into the treatment group and control group,60 cases in each group. The treatment group was treated with Zhu’s Qingre Huayu Recipe,and the control group was treated with Xuefu Zhuyu Capsule,with a course of 3 months. The changes on the scores of pelvic related pain,Chinese medical syndrome score,serum carbohydrate antigen-125 (CA-125),intercellular adhesion molecule-1 (ICAM-1) and pelvic cyst were observed and compared. Results:①Finally 117 cases completed the trial,among 60 cases in the treatment group and 57 cases in the control group. ②After treatment,there were statistically significant differences on the scores of dysmenorrhea,hypogastralgia in non-menstrual period and dyschezia between the two groups,and the scores of the treatment group were obviously lower than those of the control group(P<0.05). ③For the comparison of difference of treatment before and after,the difference on Chinese medical syndrome score in the treatment group was more greater than that in the control group(P<0.05). ④After treatment,there was statistically significant difference on the ICAM-1 level between the two groups,and the ICAM-1 level of the treatment group was obviously lower than that of the control groups(P<0.05). But there was no statistically significant difference on the CA-125 level between the two groups(P>0.05). ⑤There was a linear and positive correlation between the syndrome score of intermingled blood stasis and heat and serum ICAM-1 level. ⑥For the comparison of difference of treatment before and after between the two groups,the difference on radial line of pelvic cyst was not statistically significant(P>0.05). Conclusion:Zhu’s Qingre Huayu Recipe shows good efficacy in the treatment of pelvic related pain due to endometriosis with syndrome of intermingled blood stasis and heat,which can significantly alleviate the pain and improve the clinical symptoms. Its mechanism may be associated with reduction of ICAM-1 that related to pelvic inflammatory adhesion.关键词：endometriosis;pelvic pain;clearing heat and removing stasis;dredging Chong and Ren Channel;taking the removing obstruction as key;empirical formula of famous doctor
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发布时间：2022-08-31 - 摘要：Objective:To discuss the effects of Taibao Decoction on chorionic gonadotropin,estrogen and progesterone in threatened abortion patients with syndrome of yang deficiency of spleen and kidney. Methods90 threatened abortion patients with syndrome of yang deficiency of spleen and kidney were randomly divided into the control group and observation group,45 cases in each group. The control group was treated with progesterone by intramuscular injection,and the observation group was treated with Taibao Decoction based on the treatment for the control group,with a course of 2 weeks. The changes on clinical efficacy,syndrome scores and levels of human chorionic gonadotropin(HCG),estradiol and progesterone were observed in both groups. Results:①The total effective rates were 82.22% in the observation group and 64.44% in the control group. The clinical efficacy of the observation group was obviously better than that of the control group(P<0.05). ②After treatment,the clinical syndrome scores were obviously decreased in both groups(P<0.05),and the scores of the observation group were lower than those of the control group(P<0.05).③After treatment,the levels of HCG,estradiol and progesterone were obviously increased in both groups compared with treatment before(P<0.05),and the levels in the observation group were higher than those of the control group(P<0.05). Conclusion:Taibao Decoction shows good clinical effects on threatened abortion with syndrome of yang deficiency of spleen and kidney,which can increase the levels of human chorionic gonadotropin,estrogen and progesterone.关键词：threatened abortion;habitual abortion;syndrome of yang deficiency of spleen and kidney;Taibao Decoction
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发布时间：2022-08-31 - 摘要：Objective:To study the effects of baicalin and astragaloside IV on the formation of Pseudomonas aeruginosa biofilm. MethodsThe Pseudomonas aeruginosa PAO1 standard strains were selected,and baicalin and astragaloside IV were used to intervene culture of PAO1 biofilm in vitro,with azithromycin as the positive control and 0.9% NaCl solution as the negative control. The morphology of biofilm under different culture conditions was observed by confocal microscopy after FITC-ConA staining. Meanwhile,the total RNA was extracted by TRIzol and the expression of LasI mRNA was detected by RT-PCR. The content of glycocallyx(GXL)in PAO1 biofilm was determined. Results:①For the comparison of the effects of intervening in biofilm TE (structure-entropy) and biofilm thickness and inhibiting GLX production and LasI mRNA expression of quorum-sensing(QS)system,there were significant differences between the normal saline group and baicalin or baicalin+astragaloside IV or azithromycin group(P<0.01),but there were no significant differences between the normal saline group and astragaloside IV group(P>0.05). ②For the comparison of the effects of intervening in biofilm TE (structure-entropy) and biofilm thickness and inhibiting LasI mRNA expression of QS system,there were no significant differences between the baicalin group and azithromycin group(P>0.05),but the effect of inhibiting GLX production in the baicalin group was weaker than that in the azithromycin group(P<0.05). For the comparison of the effects of intervening in biofilm TE (structure-entropy) and biofilm thickness and inhibiting GLX production and LasI mRNA expression of QS system,the effects in the baicalin+astragaloside IV group were weaker than those in the azithromycin group(P<0.05 or P<0.01). And there were no significant differences on these effects between the astragaloside IV group and azithromycin group(P>0.05). Conclusion:Baicalin shows the obvious effects of intervening in Pseudomonas aeruginosa biofilm formation,reducing GLX production and inhibiting LasI mRNA expression,while astragaloside IV shows no obvious effects.关键词：Pseudomonas aeruginosa;biofilm;baicalin;Astragaloside IV
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发布时间：2022-08-31 - 摘要：Objective:To observe the influence of high glucose on the vitality and apoptosis of mouse podocytes cultured in vitro and the protective effects of berberine. MethodsImmortalized mouse podocytes were induced to differentiation and maturation by temperature conditioned culture. The cells were divided into the normal glucose(NG)control group(5 mmol/L),mannitol(MA)hypertonic control group(30 mmol/L),high glucose(HG)model group(30 mmol/L)and berberine(BBR) treatment group(30 mmol/L high glucose + 1.25 μmol/L BBR). After intervention for 24,8 and 72 hours,the cells were collected. The vitality of podocytes was detected by MTT assay,the cell apoptosis rate was analyzed by flow cytometry,and the mRNA expression levels of podocin and nephrin were determined by Q-PCR. Results:There were no statistically significant differences on the cell vitality,apoptosis rate and mRNA expression levels of apoptosis related molecules between the NG control group and MA control group(P>0.05). Compared with the NG control group,the vitality of podocytes was significantly decreased and the apoptosis rate was significantly increased in the HG model group(P<0.01),and the mRNA expressions of podocin and nephrin were obviously inhibited(P<0.01),and the effects of high glucose stimulation could increase over time. Compared with the HG model group,the vitality of podocytes was significantly increased and the apoptosis rate was significantly decreased in the BBR treatment group(P<0.01),and the mRNA expression levels of podocin and nephrin were obviously increased(P<0.01). Conclusion:The high glucose conditions can induce the mouse podocyte apoptosis,and this effect is independent of the hypertonic environment. Berberine shows protective effects on high glucose induced podocyte apoptosis.关键词：podocyte;cell vitality;cell apoptosis;berberine
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发布时间：2022-08-31 - 摘要：Objective:To study the effects of Zuogui Drink on the morphology of liver and kidney tissues and expression of apoptosis related proteins in D-galactose induced subacute aging rats,and discuss its mechanism of anti-aging. Methods72 healthy SD rats(half male and half female)were randomly divided into the control group,model group,vitamin E group,Liuwei Dihuang Pill group and Zuogui Drink groups with high- and low-dose. Except the control group that treated with 0.9% NaCl solution by subcutaneous injection at the neck back,the other groups were treated with D-galactose by continuously subcutaneous injection at the neck back to establish the subacute aging model. During the modeling,the rats in the drug treatment groups were treated with vitamin E,Liuwei Dihuang Pill and Zuogui Drink with high- and low-dose by intragastric administration,respectively,and the control and model group were treated with distilled water at equal volume. After administration for continuous 8 weeks,the liver and kidney tissue of rats were collected and made into the pathological sections for microscopic observation after HE staining. The positive expression levels of apoptosis related proteins(Bax,Bcl-2 and Caspase-3)in the liver and kidney tissue were detected by immunohistochemistry,and the IOD values were analyzed and compared. Results:①Compared with the control group,there were obvious pathological injuries of liver and kidney tissue in the model group. Compared with the model group,Zuogui Drink could alleviate the pathological damage of liver and kidney tissue in the aging process induced by D-galactose in rats. ②Compared with the control group,the IOD values of Bax and Caspase-3 in the liver were significantly increased in the model group(P<0.01),and the IOD value of Bcl-2 was significantly decreased(P<0.01). Compared with the model group,the IOD values of Bax and Caspase-3 in the liver were significantly decreased in the Zuogui Drink group with high-dose(P<0.01),and the IOD value of Bcl-2 was significantly increased(P<0.01). Compared with the model group,the IOD values of Bax and Caspase-3 in the liver were significantly decreased in the Zuogui Drink group with low-dose(P<0.05),the IOD value of Bcl-2 was slightly increased,but with no statistically significant difference(P>0.05). ③Compared with the control group,the IOD values of Bax and Caspase-3 in the kidney were significantly increased in the model group(P<0.01),and the IOD value of Bcl-2 was significantly decreased(P<0.01). Compared with the model group,the IOD values of Bax and Caspase-3 in the kidney were obviously decreased in the Zuogui Drink group with high-dose(P<0.01),and the IOD value of Bcl-2 was obviously increased(P<0.01). Compared with the model group,the IOD value of Bax in the kidney was obviously decreased in the Zuogui Drink group with low-dose(P<0.05),but there were no statistically significant differences on the IOD values of Bcl-2 and Caspase-3(P>0.05). Conclusion:Zuogui Drink shows a certain protective effects on the liver and kidney tissue,and its mechanism may be associated with the expression of apoptosis related proteins in the liver and kidney tissue.关键词：Zuogui Drink;tonifying kidney yin;cell apoptosis
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发布时间：2022-08-31 - 摘要：Objective:To observe the influence of Chinese medicine decoction,that with the effects of activating blood and removing stasis and supplementing essence and marrow,on the protein expressions of Kelch-like epichlorohydrin-associated protein 1(Keap1),nuclear factor erythroid 2-related factor 2(Nrf2)and heme oxygenase-1(HO-1)in experimental model rats with cerebral hemorrhage. Methods64 male Wistar rats were randomly divided into the sham operation group,model group,positive control group(Naoxuekang Capsule) and Chinese medicine decoction group,16 rats in each group. The rat model of cerebral hemorrhage was established by injection of autologous blood into the caudate nucleus. Each group was treated with the corresponding drugs once a day. 3 days later,the rats were anesthetized,the brain tissue was collected and the water content was detected. The morphological changes of nerve cells in caudate nucleus of rat brain were observed by HE staining. The expressions of Keap1,Nrf2 and HO-1 in brain tissue of rats in each group were detected by immunohistochemistry. Results:Compared with the sham operation group,the brain water content of rats in the model group was obviously increased(P<0.05)；compared with the model group,the brain water content was obviously decreased in the positive control group and Chinese medicine decoction group,and the decrease in the decoction group was more significant(P<0.05).Compared with the sham operation group,a large amount of red blood cell infiltration in the hematoma area of brain caudate nucleus were observed in rats of the model group,the number of nerve cells decreased and the glial cells increased with obvious interstitial edema,and the nerve cells presented disordered and sparse arrangement with light staining. Compared with the model group,there were a small amount of red blood cell infiltration in the brain caudate nucleus of rats in the positive control group and Chinese medicine decoction group,the nerve cells slightly swelled and loosely arranged with mild interstitial edema. Compared with the sham operation group,the expressions of Keap1,Nrf2 and HO-1 in brain tissue of rats were increased in the model group(P<0.01)；compared with the model group,the expressions of Keap1,Nrf2 and HO-1 in brain tissue of rats were decreased in the positive control group and Chinese medicine decoction group(P<0.01),and the improvement in the decoction group were more significant than those in the positive control group(P<0.05). Conclusion:Chinese medicine decoction with effects of activating blood and removing stasis and supplementing essence and marrow can improve the oxidative stress state in brain tissue by Keap1-Nrf2-HO-1 pathway,and thus protect the brain tissue from secondary injury after cerebral hemorrhage.关键词：cerebral hemorrhage;therapy of activating blood and removing stasis and supplementing essence and marrow;Keap1-Nrf2/HO-1
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发布时间：2022-08-31 - 摘要：Objective:To discuss the effects of Wenyang Bushen Decoction on Toll-like receptors(TLR)signaling mediated bone marrow dendritic cells in the pathogenesis of asthma by observing the expressions of dendritic cells in bone marrow of asthmatic mice and TLR2 and TLR4 expressions in dendritic cells. MethodsFifty BALB/c mice were randomly divided into the normal control group,model group,Chinese medicine group,western medicine group and integrated medicine group,10 mice in each group. Each group was performed the corresponding interventions. The sections of lung tissue were stained with hematoxylin eosin (HE), and the morphological structure and inflammatory infiltration were observed. The contents of IL-4,IL-5 and IFN-γ in bronchoalveolar lavage fluid were detected by enzyme linked immunosorbent assay (ELISA). The bone marrow dendritic cells of mice in each group were isolated and cultured,and the morphological changes of dendritic cells were observed under inverted phase contrast microscope and scanning electron microscope. The mRNA expressions of TLR2 and TLR4 in bone marrow dendritic cells were detected by RT-PCR. Results:The lung tissue pathology showed that the airway mucosa of mice in the model group was thickened and there was obvious inflammatory cell infiltration around it. But the inflammation was alleviated in all treatment groups in different degrees. Compared with the normal control group,the IL-4 content in bronchoalveolar lavage fluid was increased and the INF-γ content was decreased in the model group,with statistically significant differences(P<0.05). Compared with the model group,the IL-4 content was decreased in the Chinese medicine group,western medicine group and integrated medicine group,with statistically significant differences(P<0.05)；and the INF-γ content was increased in the Chinese medicine group and integrated medicine group,with statistically significant differences(P<0.05).The mRNA expressions of TLR2 and TLR4 in bone marrow dendritic cells in the model group were obviously higher than those in the normal control group(P<0.05)；all drug treatments could down-regulate the mRNA expressions of TLR2 and TLR4 in bone marrow dendritic cells,with statistically significant differences compared with the model group(P<0.05). Conclusion:Wenyang Bushen Decoction can reduce the airway resistance and alleviate the airway inflammatory response in asthmatic mice. It may mediate the signal transduction of BMDC-TLR2/TLR4 pathway,and thus significantly up-regulate Th1 cytokines secretion and down-regulate Th2 cytokines secretion,and maintain the immune balance of Th1/Th2.关键词：bronchial asthma;bone marrow dendritic cells;Wenyang Bushen Decoction
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发布时间：2022-08-31 - 摘要：Long noncoding RNA (lncRNA),as competitive endogenous RNA (ceRNA),can interact with miRNA(lncRNA/miRNA/mRNA)and play the role in the regulation of hepatocellular carcinoma. This paper reviews the functions and mechanisms of lncRNA/miRNA/mRNA in the occurrence and development of hepatocellular carcinoma,and researches about lncRNA/miRNA/mRNA regulation pathway and Chinese medicine mechanism. lncRNA/miRNA/mRNA enrich the occurrence and development mechanism of hepatocellular carcinoma,and provide novel insight into the diagnosis and therapy of hepatocellular carcinoma.关键词：lncRNA;ceRNA;miRNA;hepatocellular carcinoma;Chinese medicine mechanism;review
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发布时间：2022-08-31 - 摘要：This paper reviews the research status of chemical constituents,pharmacological activities and toxic side effects of Rhizoma Corydalis. At present,more than 40 kinds of alkaloids as well as a large number of non-alkaloid substances have been separated and identified from Rhizoma Corydalis. Except the analgesic,sedative and antithrombotic effects,it also shows the pharmacological activities such as anti-tumor,anti-diabetes and diabetic complications,improving the body resistance to stress. With the widespread application of Rhizoma Corydalis,there are a few studies on its toxic side effects.
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发布时间：2022-08-31
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