最新刊期
期 1 ， 2016
- 摘要：This paper introduces Master Qiu Peiran’s clinical experience in the prescription of herbal paste, including accurate differentiation of diseases, comprehensive treatment of big and heavy prescription, nourishment of spleen and kidney, combined use of reinforcing and reducing methods, combination of heat and cold herbs, integration of static and motive herbs, and joining of astringing and dispersing herbs. One case of duodenal ulcer treated by herbal paste was presented as an example.关键词：Qiu Peiran;herbal paste;experience of famous doctor
- 7
- |
- 590
- |
- 0
发布时间：2022-08-31 - 摘要：This article introduces Professor Shi Yangshan’s theory of “Prioritizing Qi, Blood First” and its clinical application. “Prioritizing Qi” is considered a common guideline and “Blood First” is an alteration. “Prioritizing Qi” constitutes of the methods of tonifying Qi, regulating Qi, and tonifying the viscera of spleen and kidney, which is consistent throughout the length of therapy. “Blood First” consists of varied extent of activating blood and dissipating blood stasis, aiming to alleviate symptoms at early stages. 3 clinical cases are provided.关键词：Qixue theory;Shi’s traumatology;orthopedic trauma;experience of famous doctors;Shi Yangshan
- 5
- |
- 381
- |
- 0
发布时间：2022-08-31 - 摘要：In view of intercrosses and merges of life science and information science, this paper describes the clinical bottleneck of “Preventive Treatment of Disease” in TCM now days and discusses the solution for it. It is believed that through the comparison of “Preventive Treatment of Disease” and “Treatment of Certain Disease”, introduction of disease risk assessment process in epidemiological modern health management is helpful for reinforcement the clinical application of “Preventive Treatment of Disease” in TCM; through the mixture of “Preventive Treatment of Disease” physique and disease related model which on the combination of disease risk assessment and TCM constitution identification system, we can reveal the clinical essence of “Preventive Treatment of Disease” in TCM, and it is one of the keys for improving clinical efficacy. As a interdisciplinary science solution, big data cloud computing platform, which was built by the combination of big data computing learning electronic physiology of meridians disease risk assessment process and TCM constitution identification system, can lay a foundation for the realization of internet+ TCM “Preventive Treatment of Disease” system.关键词：“Preventive Treatment of Disease”;disease risk assessment;relation between physique and disease;electronic physiology of meridians;health risk assessment questionnaire;computing learning;big data mining
- 5
- |
- 745
- |
- 0
发布时间：2022-08-31 - 摘要：This paper explores the therapy and syndromes of fearing cold due to depression syndrome. It indicates that apart from the etiology and pathogenesis of exuberance yin with yang deficiency, accumulation and obstruction of yang-qi, cold accumulation with blood stagnation, disharmony between ying and wei, and qi and blood, heat invading inward and causing yang-qi to be stagnated, as well as depression syndrome can all lead to fearing cold. Fearing cold due to depression syndrome is induced by emotions, it has mental symptoms and colorful simultaneous phenomenon, the position and nature of partial symptoms are unclear, fearing cold itself is weird and inflated, treating from the aspect of depression syndrome is worked. All mentioned above prompts that fearing cold due to depression syndrome has similar characteristics as depression syndrome. Fearing cold due to depression syndrome is a kind of masked depression syndrome which concealed by the general cloak of fearing cold, and it is difficult to be diagnosed. Fearing cold due to depression syndrome can be found in many mental disorders, such as melancholia,anxiety, and somatoform disorders, the pathogenesis of it is related to autonomic dysfunction.关键词：aversion to cold;fearing cold;depression syndrome;masked depression syndrome;soothing the liver and regulating the flow of qi
- 5
- |
- 504
- |
- 0
发布时间：2022-08-31 - 摘要：Objective:To analyze the relationship between Circulating Tumor Cells(CTC) and clinical characteristics in patients with NSCLC.Methods69 NSCLC patients who had a definite pathological diagnosis were included,and the CTC was detected by using the method of microfluidic. The correlation analysis was carried out with the clinical stage,smoking status,pathological types and so on； Results①Of the 69 patients,the positive rate of CTC was 97.10% (CTC>=1per 2ml blood). ②The CTC count of patients with stage Ⅰ-Ⅱ vs. stage Ⅲ, stage Ⅲ vs stage Ⅳ was not significantly correlated with clinical stage (P>0.05). But the CTC count that between stage I-Ⅱ and stage IV was significantly correlated(P<0.05),and so was to stage Ⅰ-Ⅲa vs. stage Ⅲb-Ⅳ (P<0.05). ③ The results showed that there was negative relationship between CTC levels and clinical stage (stage Ⅰ-Ⅲa and stage Ⅲb-Ⅳ)(rs=-0.253,P=0.036), but the number of CTC was obviously correlated with smoking status(rs=0.475,P<0.001)；while the number of CTC was not correlated with the pathological type(rs=0.05,P=0.681)and age(rs=-0.099,P=0.416) . Conclusion:The NSCLC patients’ CTC count was negatively correlated with clinical stages, and positively correlated with smoking status. The potential use of CTC in the therapeutic evaluation,treatment as well as prognosis of NSCLC is worthy of further studying. The distribution and pathological characters of CTC is similar to that of “Hidden Pathogen”, the finding provided the novel treatment strategy for the early stage NSCLC patients.关键词：non-small cell lung cancer;circulating tumor cells;clinical stage;prognosis evaluation;hidden pathogen
- 5
- |
- 675
- |
- 0
发布时间：2022-08-31 - 关键词：drug-induced liver disease;consumptive disease;teaching ward round
- 7
- |
- 199
- |
- 0
发布时间：2022-08-31 - 摘要：This paper introduces Zhu Liangzheng’s experience in treatment of recurrent oral ulcer. This disease could be divided into syndrome of deficiency fire, excess fire, clip phlegm dampness and clip blood stasis, and each syndrome should be treated with corresponding medicine. A proven case was provided.关键词：recurrent oral ulcer;aphtha;clinical experience;Zhu Liangzheng
- 5
- |
- 225
- |
- 0
发布时间：2022-08-31 - 摘要：This paper provides four medical notes written by Wang Qingqi, a national famous TCM doctor, after induction and summarization.The four medical notes related to “all diseases originated from dampness” in internal injury, Wu Zang“function well wihle unobstructed ”, Wu Zang are “the structure of Yin and function of Yang” and spleen uourishing mucosa, and such theories are innovative.关键词：medical notes;famous TCM doctor;Wang Qingqi
- 5
- |
- 270
- |
- 0
发布时间：2022-08-31 - 摘要：This paper introduces Professor Wan Hua’s clinical experience and characteristics in the treatment of plasma cell mastitis. Professor Wan proposed eliminating phlegm and dampness theory and choose “Huanglian WenDan Decoction” as formula. She invented “tunnel-shield incision” surgical approach on the basis of external therapy combines with conventional therapy.关键词：plasma cell mastitis;eliminating phlegm and dampness method;“tunnel-shield incision” surgical approach;clinical experience;Wan Hua
- 6
- |
- 394
- |
- 0
发布时间：2022-08-31 - 关键词：elderly gastroesophageal reflux disease;senile eczema;hyperplasia of mammary gland;syndrome differentiation of constitution;Chinese classic herbal formula;proven case
- 5
- |
- 175
- |
- 0
发布时间：2022-08-31 - 摘要：This paper analyzes experience of treating epidemic febrile disease “Fluid and sweat in saving yin”. “Save yin not in blood but fluid and sweat” is Ye Tianshi’s experience in treating epidemic febrile disease. We argued that this should be explained like this:it is easy to sweat in epidemic febrile disease, so it is important to save yin according to the balance of positive and evil. On the theory of epidemic febrile disease, to save yin is to enrich fluid to sweating.
- 5
- |
- 0
- |
- 0
发布时间：2022-08-31 - 摘要：The article tried to summarize philosophy of treatment on male infertility of Doctor Yue Fujia in Ming Dynasty. Yue Fujia traced the etiology by syndrome differentiation by means of synthetically analysis of pulse and syndrome in order to master the pathological nature of diseases. He emphasized on aiming at the pathogenesis with the power of understanding and flexible ways while searching for the primary cause of diseases in treatment.关键词：Yue Fujia;infertility;academic thought
- 5
- |
- 134
- |
- 0
发布时间：2022-08-31 - 摘要：Objective:To observe the effect of “Tangzu Formula” on latent syndrome of diabetic foot tendon gangrene. Methods66 latent syndrome of diabetic foot tendon gangrene cases were randomly divided into treatment group and control group, 33 in each group. Based on the same basic treatment,the treatment group was treated with “Tangzu formula” for oral, and the control group was treated with placebo granule for oral. 1 month for each group. We observed the changes of TNF-α,Hs-CRP,IL-6,IL-2 and FPG,2hPG,HbA1C. Results:①Compared in each group before and after the treatment, there was statistic difference in 2 hPG in treatment(P<0.05) while no difference in each index in control group(P>0.05)；② Compared in each group before and after the treatment, there was statistic difference in TNF-α, Hs-CRP, IL-6 in treatment group(P<0.05), while no difference in each index in control group(P>0.05)；Compared between the two groups before and after the treatment, levels of TNF-α, Hs-CRP, IL-6 in treatment group was lower than that of control group(P<0.05). Conclusion:“Tangzu Formula” has some effect on latent syndrome of diabetic foot tendon gangrene, can decrease the index of TNF-α, Hs-CRP, IL-6, etc., which can provide better clinical treatment plan of preparation and maintenance treatment for diabetic foot tendon gangrene.关键词：diabetic foot tendon gangrene;latent syndrome;microinflammatory;“Tangzu Formula”
- 5
- |
- 195
- |
- 0
发布时间：2022-08-31 - 摘要：Objective:To observe the effects of “Tongbi Paste” on the patients with diabetic lower limb arteriosclerosis obliterans. Methods64 patients with diabetic lower limb arteriosclerosis obliterans were randomly divided into treatment group and control group, 32 in each group. The control group was treated with Tongsaimai Tablet,and the treatment group was treated with “Tongbi Paste”. The courses of the two groups were 3 months. Clinical effects of both treatments were observed, and ankle brachial pressure index, lower lib blood vessel color doppler ultrasound index, blood lipid and plasma albumin changes were compared. Results:①The total clinical efficacy rates of treatment group and control group were 90.63% and 68.75% respectively; there was significant difference in the clinical efficacy between the two groups (P<0.05). ②Compared before and after the treatment, there was significant difference in ankle brachial pressure index in each group(P<0.05). Compared before and after the treatment, there was significant difference in ankle brachial pressure index between the two groups(P<0.05). ③ Compared before and after the treatment, there was significant difference in levels of vessel diameter, peak velocity, and blood flow of dorsalis pedis artery in treatment group(P<0.05). Compared before and after the treatment, there was significant difference in levels of vessel diameter, peak velocity, and blood flow of dorsalis pedis artery between the two groups(P<0.05). ④Compared before and after the treatment, there was significant difference in levels of TC, TG, LDL-C, HDL-C and FIB in treatment group(P<0.05). Compared before and after the treatment, there was significant difference in levels of TC, TG, LDL-C, HDL-C and FIB between the two groups(P<0.05). were power of therapeutic group is 90.6% which is higher than 68.8% of control group(P<0.05). Compared with pre-treatment,ABI,blood flow volume and peak flow velocity of dorsal artery improved significantly at twelve weeks after treatment(P<0.05)；the treatment group had better effects than the control group in reducing blood lipid and fibrinogen(P<0.05). Conclusion:The clinical effect was satisfactory in the treatment of patients with diabetic lower limb arteriosclerosis obliterans with “Tongbi Paste”.关键词：herbal paste;diabetic;peripheral arterial disease;lower limb arteriosclerosis obliterans;supplementing qi and nourishing yin;promoting blood circulation to remove meridian obstruction
- 5
- |
- 301
- |
- 0
发布时间：2022-08-31 - 摘要：Objective:To observe clinical efficacy of “Five Shen Oral Liquid” combined with western medicine in the treatment of Qi Yin deficiency premature ventricular contractions(VPCs). Methods60 cases of Qi Yin deficiency VPCs patients were randomly divided into treatment group and control group, 30 cases in each group. The two groups were both treated with conventional western medicine, the control group was treated with “Sheng Mai Oral Liquid” in addition, and the treatment group was treated with “Five Shen Oral Liquid” in addition. Two groups of treatment for 30 days, observe the comparison of the number of VPCs, TCM syndrome differentiation and the change of heart rate variability parameters. Results:①Before and after the treatment, the number of VPCs of both electro cadiogram and 24 h dynamic electrocadiogram in the two groups were significantly decreased, the difference was statistically significant (P<0.05). After treatment, there were significant differences in VPCs of both DCG and ECG between the two groups (P<0.05). ②After treatment, the difference of TCM syndrome differentiation was statistically significant, and the treatment group was better than the control group (P<0.05). ③After treatment, SDNN, SDNNIDX, SDANN and rMSSD levels were significantly different between the two groups, the treatment group was better than the control group (P<0.05). Conclusion:The effectiveness of the “Five Shen Oral Liquid” combined with western medicine in the treatment of Qi Yin deficiency VPCs was satisfied,it can significantly ease the patient’s clinical symptoms and signs, the mechanism might be related to the regulation of the sympathetic and vagal nerve relative balance.关键词：arrhythmia;premature ventricular contractions(PVCs);palpitations;Qi and Yin deficiency sign;“Five Shen Oral Liquid”;heart rate variability (HRV)
- 5
- |
- 161
- |
- 0
发布时间：2022-08-31 - 摘要：Objective:To investigate the clinical efficacy of different frequency after acupuncture treatment of ischemic stroke motor dysfunction. Methods90 cases of movement disorders after ischemic stroke patients were randomly divided into slow frequency group, moderate frequency group and high frequency group, 30 in each group. Each group of the same acupuncture acupoints, acupuncture frequencies were:slow frequency group of 30 beats/min, the frequency of the group of 60 beats/min, high-frequency group 90 beats/min, every 30min, with course of 30 days for each group. The clinical efficacy, the neurological deficit score, limb motor function score and quality of life score were compared. Results:The total efficiency for slow, moderate and high frequency groups were 93.33%,100% and 93.33% respectively; moderate frequency group clinical total efficiency was significantly higher than the slow frequency group and high frequency group(P<0.05). After treatment, the neurological deficit scores of the three groups were significantly decreased than before(P<0.01), and moderate frequency group was significantly lower than the slow frequency group and high frequency group(P<0.01); the limb motor function Fugl-Meyer scores of quality of life of the three groups increased than before(P<0.01), and moderate frequency group was higher than the slow frequency group and high frequency group(P<0.05). Conclusion:Moderate frequency helps improve the clinical efficacy of acupuncture acupuncture treatment of ischemic stroke dyskinesia.关键词：ischemic stroke;movement disorders;acupuncture therapy;acupuncture frequency
- 5
- |
- 212
- |
- 0
发布时间：2022-08-31 - 摘要：Objective:To study the effect of “Quyu Jiedu Yiqi Decoction” on levels of plasma calcitonin proinsulin (PCT) and diamine oxidase (DAO) in patients with sepsis. Methods90 sepsis patients were randomly divided into control group (45 cases, conventional anti-infection and life-support therapy) and treatment group (45 cases, “Quyu Jiedu Yiqi Decoction” and conventional therapy), both with a course of two weeks; the levels of PCT and DAO were observed before and after treatment. Results:After treatment, the levels of PCT and DAO were significantly lower (P<0.05), and the treatment group was better than the control group (P<0.05). Conclusion:The conventional therapy with “Quyu Jiedu Yiqi Decoction” could mitigate inflammation and improve their intestinal mucosal injury in sepsis patients.关键词：“Quyu Jiedu Yiqi Decoction”;sepsis;inflammatory cytokines;intestinal mucosal injury
- 5
- |
- 189
- |
- 0
发布时间：2022-08-31 - 摘要：Objective:To observe the clinical efficacy of “Yiban Decoction” and Losartan for the treatment of chronic nephritis proteinuria. Methods70 patients with chronic nephritis proteinuria were randomly divided into treatment group and control group,35 in each group. Both groups were treated with Losartan, and treatment group was treated with “Yiban Decoction” additionally,with the course of three months for both groups. The clinical efficacy were observed, 24 h urinary protein, plasma protein, TCM syndrome differentiation and renal function related index were compared. Results:①The total effective rate was 62.86% in the control group and 77.1% in the treatment group, with a difference between the two groups (P<0.05). ②Comparison before and after treatment, there were significant differences in the levels of 24 hour urine protein and plasma protein between the two groups (P<0.05). ③Compared before and after the treatment, there was significant difference in the level of TCM syndrome differentiation in each group(P<0.05). Compared before and after the treatment, there was significant difference in the level of TCM syndrome differentiation between the two groups(P<0.05). ④Compared before and after the treatment, there was significant difference in the level of BUN, Cr, ALB in each group(P<0.05). Compared before and after the treatment, there was significant difference in the level of ALB between the two groups(P<0.05). Conclusion:“Yiban Decoction” and Losartan for the treatment of Qi Yin deficiency and damp-heat chronic nephritis proteinuria can decrease urinary protein and improve clinical syndrome.关键词：chronic glomerulonephritis;urinary protein;“Yiban Decoction”;Losartan;Qi Yin deficiency;damp-heat
- 5
- |
- 153
- |
- 0
发布时间：2022-08-31 - 摘要：Objective:To evaluate the effect of “Wuxing Jiangu Cao” on quality of life in patients with postmenopausal osteoporosis. MethodsTotally 78 patients with postmenopausal osteoporosis were randomly divided into control group and observation group, 39 cases in each group. The control group was treated with oral alfacalcidol and calcium carbonate D3, while the observation group was treated with “Wuxing Jiangu Cao” on the basis of the control group.180 days for both the groups. OQOLS was observed before and after treatment. Results:After the treatment, the scores of each project and total score of OQOLS in each group were significantly decreased (P<0.05), and the scores of observe group were lower than that of control group (P<0.05). Conclusion:“Wuxing Jiangu Cao” can improve quality of life in patients with postmenopausal osteoporosis.关键词：postmenopausal osteoporosis;“Wuxing Jiangu Cao”;quality of life
- 6
- |
- 195
- |
- 0
发布时间：2022-08-31 - 摘要：Objective:To observe the clinical effect of the treatment of glucocorticoid related osteoporosis with the method of invigorating the kidney and promoting blood circulation combined with western medicine. Methods60 patients with glucocorticoid related osteoporosis were enrolled in the diagnosis,randomly divided into treatment group and control group, 30 in each group. The control group was treated with a lun lin suan naVitD3 tablets plus calcium carbonate tablets, while the treatment group was treated with Chinese medicine of invigorating kidney and promoting blood on the basis of the treatment for the control group. After 6 months of treatment, two groups of visual analog (VAS) pain score, Bone metabolism index, Bone mineral density was observed and liver and kidney function was monitored. Results:① Compared before and after the treatment, there was significant difference in VAS pain score in each group (P<0.05); compared before and after the treatment, there was significant difference in VAS pain score between the two groups (P<0.05). ②Compared before and after the treatment, there was significant difference in Bone metabolism index of lumbar vertebrae and both side of the hip in each group(P<0.05). Compared before and after the treatment, there was significant difference in Bone metabolism index of lumbar vertebrae and both side of the hip between the two groups(P<0.05). ③Compared before and after the treatment, there was significant difference in levels of BGP, β-CTx, PTH and IL-6 in treatment group(P<0.05), while there was significance difference in levels of β-CTx, PTH and IL-6 in control group(P<0.05). Compared before and after the treatment, there was significant difference in levels of BGP, β-CTx and IL-6 between the two groups(P<0.05). Conclusion:The clinical effect was satisfactory in the treatment of glucocorticoid related osteoporosis with the method of invigorating the kidney and promoting blood circulation combined with western medicine.关键词：glucocorticoid;osteopenia;osteoporosis;invigorating the kidney and promoting blood circulation;bone mineral density;bone metabolism
- 5
- |
- 89
- |
- 0
发布时间：2022-08-31 - 摘要：This paper introcluces the experience of using warming Yang and recuperating the kidney method on treating insomnia of yang deficiency. The author believes that the pathogenesis of this disease is yang deficiency or lack of mind and sprit preservation which due to yang floating. The symptoms are sleeplessness, dreaminess and restlessness. The clinical effect was satisfactory by treating with Jinkui Shenqi Pill or Zuogui Pill. 3 proven cases were provided in this paper.关键词：sleeplessness;warming yang method;Jinkui Shenqi Pill;clinical experience
- 7
- |
- 312
- |
- 0
发布时间：2022-08-31 - 摘要：Objective:To Study on the differentially expressed miRNA of drug-contained serum of “Bushen Decoction” on rat intervertebral disc chondrocytes, which aims to provide target miRNA for treating cervical spondylosis with “Bushen Decoction” , and to discuss the function mechanism by regulating the target of related genes. MethodsThe primary rat intervertebral disc chondrocytes were identified by collagen type Ⅱ immunofluorescent staining and tuluidine blue staining. The intervertebral disc chondrocytes were cultured in serum containing “Bushen Decoction” or blank serum respectively. After a given time of intervention,the cultured cells were collected,and the total RNA were isolated and purified and used for follow-up miRNA microarray experiments. Real-time quantitative PCR was used to further validate the expression levels over 4-fold up-regulated. Results:The twice-enzyme digestion method was practicable,efficient and ideal technique to obtain a large number of purified primary rat intervertebral disc chondrocytes in a short time and culture cells with typical intervertebral disc chondrocytes characteristics. Collagen type Ⅱ immunofluorescent staining and tuluidine blue staining were positive. The miRNA expression profiles in “Bushen Decoction” containing serum groups compared inner the groups after comparing with that from blank serum control subjects. Compared with drug-contained serum of “Bushen Decoction” on rat intervertebral disc chondrocytes cultrured 1d, 121 over 2-fold different expression of miRNA (53 up-regulated and 68 down-regulated) were identified in serum cultured 3d. The results of PCR were in accordance with the miRNA array results that the expression levels over 4-fold up-regulated. Conclusion:A set of specific miRNA were obtained after “Bushen Decoction” containing serum intervention on rat intervertebral disc chondrocytes for 3d. Target gene prediction software to predict the up-regulated miRNA mainly by improving the nutritional metabolism of intervertebral disc through the target genes such as Opn,Col,Bcl212,Vdr,IGF-I and Mmp-13,et al.关键词：“Bushen Decoction”;intervertebral disc chondrocytes;rat miRNA array;differential expression
- 5
- |
- 214
- |
- 0
发布时间：2022-08-31 - 摘要：Objective:To observe the effect of “Xiaotan Sanjie Decoction” on the RUNX3 and NF-κB p65 protein expression in Human gastric cancer cell SGC-7901-bearing nude mice,and discuss the anti-gastric cancer effect and mechanism of “Xiaotan Sanjie Decoction”. MethodsHuman gastric cancer cell SGC-7901-bearing nude mice models were made. The mice were divided into TCM group (“Xiaotan Sanjie Decoction”),chemotherapy group (capecitabine) and control group (0.9%NaCl saline) randomly, 10 in each group, with 32 d intervention respectively in each group. The tumor volume,tumor weight, body weight, RUNX3 and NF-κB p65 protein expression in tumor were observed the the method of Western blotting. Results:The tumor volume and weight of TCM group and chemotherapy group were lower than those of control group(P<0.05); compared with TCM group, the tumor volume and weight in chemotherapy group decreased, and the difference was statistically(P<0.05); The anti-tumor rate of TCM group and chemotherapy group were 44.91% and 83.23% respectively. There was no statistical significance in weight between TCM group and control group(P>0.05). The body weight in chemotherapy group decreased compared with that in control group and TCM group(P<0.05). The RUNX3 protein expression in TCM group and chemotherapy group was higher than that in control group,while the NF-κB p65 expression was lower than that of control group(P<0.05). Conclusion:“Xiaotan Sanjie Decoction” has the good anti-gastric cancer effect. Although the anti-cancer rate of “Xiaotan Sanjie Decoction” was not as good as capecitabine,it had not bodyweight decreasing side effect. Increasing the RUNX3 expression and decreasing the NF-κB P65 protein expression in tumor tissues may account for the anti-gastric cancer mechanism of “Xiaotan Sanjie Decoction”.关键词：“Xiaotan Sanjie Decoction”;nude mice;gastric cancer;RUNX3;NF-κB p65
- 5
- |
- 229
- |
- 0
发布时间：2022-08-31 - 摘要：Objective:To observe the influence of Bushen Yiqi Decoction and aerobic exercise on the expression of mitochondria biosynthesis related signaling molecules p38MAPK,CaMKⅡ,PGC-1α,MEF2C and COXⅣ in D - galactose-induced aging rats’ skeletal muscle. Methods40 male SD rats were randomly divided into five groups:normal group, model group, traditional Chinese medicine group, exercise and traditional Chinese medicine group and methandienone group. The rats were received a subcutaneous injection of 10% D-galactose solution(100 mg·kg-1,once a day)for 10 weeks to make the aging model except normal group rats. Bushen Yiqi Decoction, aerobic exercise and methandienone were used to the corresponding experimental groups to intervene for 10 weeks. β-Galase of blood serum were tested by ELISA; Calcium content of gastrocnemius mitochondria were tested by Methyl thyme phenol blue (MTB); The expression of mitochondrial biosynthesis related signal transduction molecules (p38MAPK,CaMKⅡ,PGC-1α,MEF2C and COXⅣ) were tested by Western blot respectively. Results:Compared with normal group, model group rats’ aging biology markers β-Galase, calcium content in mitochondria increased significantly. The protein expression (protein) of model rats’ CaMKⅡ, MEF2C, PCG-1 alpha decreased obviously, and the protein expression of P38MAPK increased significantly. The Bushen Yiqi Decoction combined with aerobic exercise could obviously decrease calcium content, and increase the protein expression of CaMKⅡ, PGC-1α, MEF2C and COXⅣ of the model aging rats. The methandienone could obviously increase the protein expression of PCG-1α and COXIV of the model aging rats, decrease calcium content obviously. Conclusion:The decline of aging model rats’ skeletal muscle function may related to expression changes of mitochondria biosynthesis related signal transduction pathways molecular CaMKⅡ, p38MAPK, PCG-1 alpha, MEF2C and energy metabolism related genes (COXIV). The Bushen Yiqi Decoction combined with aerobic exercise can delay age-related degradation of the skeletal muscle in some degree through improving the abnormal changes of the above index.关键词：Bushen Yiqi Decoction;aerobic exercise;aging rats;skeletal muscle;mitochondria biosynthesis
- 5
- |
- 338
- |
- 0
发布时间：2022-08-31 - 摘要：Objective:To discuss bakuchiol on melanin synthesis and its intervention of MAPK signaling transduction pathways in A375 cells. MethodsThe cultured A375 cells were treated with bakuchiol; MTT assay,L-dopa oxidation assay were respectively used to detect the activity of cell and TYR ; odium hydroxide lysis assay were used to detect the change of melanin synthesis ; RT-PCR was applied to measure the mRNA expression levels of TYR,TRP-1,TRP-2 and the mRNA expression levels of JNK2,ERK1,ERK2. Results:bakuchiol (10-1 μmol/L,10-2 μmol/L,10-3 μmol /L) can inhibit melanin synthesis and the activity of TYR, Bakuchiol(10-1 μmol/L) can significantly reduce the mRNA expression level of TYR, TRP-1,TRP-2,JNK2,ERK1 and ERK2(P<0.05,P<0.01). Conclusion:Bakuchiol can reduce melanin synthesis and the activity of TYR,and the changes realized probably through reducing the mRNA expression level of TYR,TRP-1,TRP-2,JNK2,ERK1 and ERK2 .关键词：bakuchiol;MAPK pathway;melanin synthesis;A375 cells
- 5
- |
- 254
- |
- 0
发布时间：2022-08-31 - 摘要：Objective:To optimize ethanol reflux extraction process of total triterpenoids in Ligustrum lucidum Ait. MethodsOn the basis of single factor tests,response surface analysis of three-factor-three-level was adopted to investigate effects of ethanol concentration,extracting time and solvent fold on yield of total triterpenoids. Results:Optimal extraction conditions were as follows:extracted 2 times by 12 times the count of 67% ethanol with 1.70 h each time; under these conditions,yield of total triterpenoids was 57.62 mg/g,2.89% in low deviation than predicted. Conclusion:This extraction process of total triterpenoids from L. Lucidum dahurica by central composite design/response surface methodology was a simple method with high precision and good predictability.关键词：Ligustrum lucidum Ait.;total triterpenoids;response surface methodology;ethanol reflux extraction
- 5
- |
- 360
- |
- 0
发布时间：2022-08-31 - 摘要：This paper review the general situation of traditional Chinese medicine for reversing multidrug resistance of breast cancer. At present, such traditional Chinese medicine for reversing multidrug resistance of breast cancer were studied mostly, which include:monomer and extract of Traditional Chinese Medicine,like turmeric,radix ginseng,Radix Sophorae Flavescentis and so on,compound traditional Chinese medicine such as compound Zilongjin,reverse capsule,Shugan Yishen recipe. However,few studies in vitro and in compound research,the shallow mechanism to investigate is still the main problem of reversing the multidrug resistance of breast cancer.关键词：breast cancer;multidrug resistance;traditional Chinese medicine;reversing multidrug resistance
- 5
- |
- 916
- |
- 0
发布时间：2022-08-31 - 摘要：This paper reviews the progress on pharmacological activity of inonotus obliquus polysaccharide.Inonotus obliquus polysaccharide has the ability of reducing blood sugar, lowering blood fat, regulatiing immune, anti-tumor, anti-radiation, anti-oxidation, anti-parasitic and so on.关键词：inonotus obliquus polysaccharide;pharmacological activity;research
- 5
- |
- 827
- |
- 0
发布时间：2022-08-31
- Technical support is provided by Beijing Founder electronics co., LTD 沪ICP备13044091号
沪公网安备 31011502015228号 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
- Total Visits：70575 Visits Today：56
0