最新刊期
期 3 ， 2015
- 摘要：Objective: To observe the clinical efficacy of “Gengnian Ningxin Granules” in treating perimenopausal syndrome. Methods Sixty cases with perimenopausal syndrome were randomized into treatment group and control group, with 30 cases in each group. Treatment group was treated with “Gengnian Ningxin Granules”, and the control group was treated with placebo, with the course of 2 months. The modified Kupperman index, hot flashes times and levels of sex hormones (FSH, LH, E2) were observed. Results: ① Non-shedding case during the period of the clinical trial. All the 60 cases completed the study. ② In both groups, there was significant difference in KI score between before and after the treatment (P＜0.05); after treatment, there was significant difference in KI score between the two groups (P＜0.05). ③ In both groups, there was significant difference in hot flashes times between before and after the treatment (P＜0.05); after treatment, there was significant difference between the two groups (P＜0.05). ④ In both groups, no significant difference was found in the levels of sex hormones (E2, FSH, LH) between before and after the treatment (P＞0.05); after treatment, no significant difference was found in the levels of E2, FSH and LH between the two groups (P＞0.05). Conclusion: “Gengnian Ningxin Granules” is effective to improve the clinical symptoms in patients with perimenopausal syndrome. Its mechanism may be not association with the improvement of estrogen level.关键词：perimenopausal syndrome;“Gengnian Ningxin Granules”;clearing heart and soothing liver method;placebo
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发布时间：2022-08-31 - 摘要：Objective: To observe the clinical efficacy of minimally invasive thread-embedding at acupoints combined with western medicine in treating moderate to severe chronic obstructive pulmonary disease (COPD) in stable phase. Methods Two hundred patients with moderate to severe COPD in stable phase were randomly divided into the treatment group and control group, with 100 cases in each group. The control group was treated with conventional western medicine, and the treatment group was treated with minimally invasive thread-embedding at acupoints additionally, with the course of one year. The times of acute exacerbation of COPD (AECOPD), severe exacerbations, SGRQ score, Hamilton Anxiety Scale (HAMA), Hamilton Depression Scale (HAMD), 6 minutes walking distance (6MWD), and lung function (FEV1, FEV1/FVC), as well as the safety indexes were observed. Results: ① One hundred and eighty-five patients were followed up for 1-year, 7 patients were lost in the treatment group, while 8 patients were lost in the control group. ②After treatment, there were significant differences in AECOPD, number of severe exacerbations, SGRQ score, HAMA, HAMD, and 6MWD between the two groups (P＜0.05). ③ After treatment, no significant difference was found in the levels of FEV1 and FEV1/FVC between the two groups (P＞0.05). ④ No significant difference was found in adverse reaction rate between the two groups (P＞0.05). Conclusion: Combined minimally invasive thread-embedding at acupoints and western medicine is effective to reduce the number of AECOPD, and improve the clinical symptoms, activity endurance, and relieve depression and anxiety in patients with moderate to severe COPD in stable phase.关键词：chronic obstructive pulmonary disease (COPD);stable phase;moderate or severe;minimally invasive thread-embedding at acupoint;acute exacerbation;6 minutes walking distance (6MWD)
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发布时间：2022-08-31 - 摘要：Objective: To observe the clinical efficacy of “Yiqi Huoxue Zhuanggu Formula” and western medicine in treating coronary heart disease (CHD) in stable phase complicated with osteoporosis. Methods Two hundred cases with stable CHD complicated with osteoporosis were randomized into treatment group and control group by random number table, with 100 cases in each group. Control group was treated with western medicine, and treatment group was treated with “Yiqi Huoxue Zhuanggu Formula” additionally, with the course of 3 months. The clinical efficacy, and changes of bone mineral density (BMD), bone metabolism indexes (CTX-1, BGP) and cardiac function indexes (LVEF, LVEDP) were observed and compared before and after treatment. Results: ① One hundred finished the trail. Ten cases in the treatment group were lost, and 10 cases in the control group were lost. ② The total effective rate was 91.11% in the treatment group and 80.00% in the control group, with significant difference between the two groups (P＜0.05). ③ In both groups, there were significant differences in the levels of BMD, CTX-1 and BGP, and cardiac function indexes (LVEF, LVEDP) between before and after treatment (P<0.05); after treatment, there were significant differences in the levels of BMD, CTX-1, BGP and cardiac function indexes (LVEF, LVEDP) between the two groups (P<0.05). Conclusion: Combined “Yiqi Huoxue Zhuanggu Formula” and western medicine is effective to treat coronary heart disease (CHD) complicated with osteoporosis by improving bone mineral density, bone metabolism and cardiac function.关键词：osteoporosis;stable coronary heart disease;“Yiqi Huoxue Zhuanggu Formula”;bone mineral density;bone metabolism
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发布时间：2022-08-31 - 摘要：Objective: To observe the clinical efficacy of “Jianpi Huoxue Sanzhuo Decoction” in treating hyperlipemia. Methods One hundred and two cases with hyperlipemia were randomized into the treatment group and control group, with 51 cases in each group. The control group was treated with “Xuezhikang Capsules” orally, and the treatment group was treated with “Jianpi Huoxue Sanzhuo Decoction” orally, with the course of 8 weeks. The clinical efficacy, changes of blood lipid-related indicators (TC, TG, HDL-C, LDL-C), atherogenic index (AI) and hemorheological indicators (blood viscosity and plasma viscosity) were observed. Results: ① The total effective rate was 94.12% in the treatment group and 78.43% in the control group, with a difference between the two groups (P<0.05). ② In both groups, there were significant differences in the levels of TC, TG, HDL-C, LDL-C and AI between before and after treatment (P＜0.05); after treatment, there were significant differences in the levels of TC, TG, HDL-C, LDL-C and AI between the two groups (P＜0.05). ③In both groups, there were significant differences in blood viscosity and plasma viscosity between before and after treatment (P＜0.05); after treatment, there were significant differences in blood viscosity and plasma viscosity between the two groups (P＜0.05). Conclusion: “Jianpi Huoxue Sanzhuo Decoction” has positive effect on hyperlipemia.关键词：hyperlipemia;“Jianpi Huoxue Sanzhuo Decoction”;“Xuezhikang Capsules”;atherogenic index (AI);blood viscosity
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发布时间：2022-08-31 - 摘要：Objective: To observe the clinical efficacy of “Qufeng Xingxue Tongluo Formula” in treating ischemic stroke and to explore its effect on platelet function. Methods Eighty cases with ischemic stroke were randomized into two groups:control group in which 40 cases were treated with conventional western medicine, and treatment group in which 40 cases were treated with “Qufeng Xingxue Tongluo Formula” additionally, with the course of 2 weeks. The degrees of neurologic impairment, spasm of limbs, motor function of limbs and changes of blood platelet parameters were observed. Results: ① After treatment, scores of Ashworth and NIHSS were decreased in both groups (P<0.05), and FMI score was increased (P<0.05); scores of Ashworth and NIHSS in the observation group were lower than those of the control group (P<0.05), while the FMI score in observation group was higher than that of the control group (P<0.05); ② After treatment, the levels of thromboxane, homocysteine and von Willebrand factor were reduced in both groups (P<0.05), while the 6-keto - prostaglandin level was increased (P<0.05); the levels of thromboxane, homocysteine and von Willebrand factor of the observation group were lower than those of the control group (P<0.05), and the 6-keto - prostaglandin level of the observation group was higher than that of the control group (P<0.05); ③ After treatment, the platelet aggregation rate and the mean platelet volume (MPV) decreased in both groups (P<0.05), and the indicators of the observation group were lower than those of the controls (P<0.05). Conclusion: “Qufeng Xingxue Tongluo Formula” is effective to improve the clinical symptoms and neurologic impairment in patients with ischemic stroke. Its mechanism may be association with improving the function of blood platelets and inhibiting the thrombosis.关键词：ischemic stroke;“Qufeng Xingxue Tongluo Formula”;function of blood platelet;neurologic impairment
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发布时间：2022-08-31 - 摘要：Objective: To observe the clinical efficacy of supplemented “Fuling Zexie Decoction” for the treatment of vertigo caused by posterior circulation ischemia of the phlegm turbidity obstructing the center pattern. Methods Eighty cases with vertigo caused by posterior circulation ischemia were randomly divided into the treatment group and control group, with 40 cases in each group. Both groups were treated with Ozagrel intravenously; in addition, the control group was treated with Nimodipine Tablets orally, and the treatment group was treated with supplemented “Fuling Zexie Decoction”, with the course of 10 days. Results: The total effective rate was 97.5% in the treatment group and 80.0% in the control group, with a difference between the two groups (P<0.01). After treatment, scores of the primary symptom were reduced in both groups (P<0.01), and scores in the treatment group were lower than that of the controls (P<0.01). Conclusion: Supplemented “Fuling Zexie Decoction” has positive clinical effect on vertigo caused by posterior circulation ischemia.关键词：vertigo;posterior circulation ischemia;“Fuling Zexie Decoction”
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发布时间：2022-08-31 - 摘要：Objective: To observe the clinical efficacy of “Yiqi Guben Tiaomian Formula” in treating IgA nephropathy of the spleen kidney qi-deficiency pattern. Methods Seventy-nine cases with IgA nephropathy of the spleen kidney qi-deficiency pattern were randomized into two groups. Forty cases in the control group were treated with conventional therapy, and 39 cases in the treatment group were added with “Yiqi Guben Tiaomian Formula” orally, with the course of 2 months. Results: ① The total effective rate was 84.6% in the treatment group and 57.5% in the control group, with a difference between the two groups (P<0.01). ② After treatment, the levels of U-RBC and 24h urine protein were decreased in both groups (P<0.05, P<0.01), and the level of the treatment group was lower than that of the controls (P<0.05). ③ After treatment, no significant difference was found in the main symptom and total symptom scores of the control group (P>0.05); symptoms such as weakness and fatigue, soreness and weakness of waist and knees, and the total score of the treatment group were reduced markedly (P<0.05, P<0.01); there were significant difference in main symptoms and total symptom scores between the two groups (P<0.01). Conclusion: “Yiqi Guben Tiaomian Formula” is effective to alleviate the clinical symptoms and proteinuria in patients with IgA nephropathy of the spleen kidney qi-deficiency pattern.关键词：IgA nephropathy;spleen kidney qi-deficiency pattern;“Yiqi Guben Tiaomian Formula”
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发布时间：2022-08-31 - 摘要：Objective: To observe the clinical efficacy of “Ningmitai Capsules” in treating urogenital system infection of damp-heat in lower energizer pattern. Methods Three hundred and sixteen cases with urogenital system infection were treated with “Ningmitai Capsules”, with the course of 7 days. The clinical efficacy and changes of symptoms score and urine routines were observed. Results: The total effective rate was 91.43%, and the negative conversion ratio of bacteria cultivation was 91.67%; after treatment, the counts of WBC and red blood cells in urine and urine culture (P<0.05, P<0.01); the total scores of clinical symptoms were reduced from (16.34±4.35) to (7.16±2.95). There was a significant difference between before and after treatment (P<0.01). Conclusion: “Ningmitai Capsules” has positive effect on urogenital system infection of damp-heat in lower energizer pattern.关键词：“Ningmitai Capsules”;urogenital system infection;damp-heat in lower energizer pattern
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发布时间：2022-08-31 - 摘要：Objective: To observe the clinical efficacy of “Man Yin Yan Ⅰ Formula” on senile vaginitis of kidney-yin deficiency pattern internally and externally. Methods One hundred cases with senile vaginitis were randomized into treatment group and control group, with 50 cases in each group. Treatment group was treated with “Man Yin Yan Ⅰ Formula” and control group was treated with Tinidazole, with the course of 14 days. The clinical efficacy and leucorrhea were observed. Results: The total effective rate was 98.0% in the treatment group and 88.0% in the control group, and the clinical efficacy of the treatment group was superior to that of the control group (P<0.05). In both groups, scores of the amount, color, smelling of leucorrhea were reduced significantly (P<0.05), and the routine test on leucorrhea was improved markedly (P<0.05). The improvement of symptoms of the treatment group was superior to that of the control group (P<0.05). Conclusion: “Man Yin Yan Ⅰ Formula” has positive effect on senile vaginitis of kidney-yin deficiency pattern by internal and external use. It is worth to apply in clinic.关键词：senile vaginitis;kidney-yin deficiency pattern;“Man Yin Yan Ⅰ Formula”
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发布时间：2022-08-31 - 摘要：Objective: To observe the clinical efficacy of herbal compress and exercise therapy in treating knee osteoarthritis (KOA). Methods Sixty-eight cases were randomized into two groups:control group in which 33 cases were treated with Voltaren Release Tablets and exercise therapy, and treatment group in which 35 cases were treated with herbal compress and exercise therapy, with the course of 14 days. The clinical efficacy, visual analogue scale (VAS) and Lequesne index were compared. Results: ① The total effective rate was 97.14% in the treatment group and 75.76% in the control group, and the clinical efficacy of the treatment group was superior to that of the control group (P＜0.05). ② The visual analogue scale (VAS) and Lequesne index were decreased after the treatment in both groups (P＜0.05), and the indexes of the treatment group were lower than those of the control group (P＜0.05). Conclusion: Combined herbal compress and exercise therapy has positive effect on knee osteoarthritis by alleviating the pain, swell and morning stiffness markedly.关键词：knee osteoarthritis (KOA);herbal compress;exercise therapy;external treatment
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发布时间：2022-08-31 - 摘要：Objective: To observe the clinical efficacy of “Bushen Shengsui Decoction” for fracture of extremities after operation and to explore its mechanism. Methods Seventy-three cases with limbs fracture were randomized into two groups and underwent reset surgery. Thirty-six cases in the observation group were treated with “Bushen Shengsui Decoction” orally, and 37 cases in the control group were treated with Ossotide Injection intravenously, with the course of 2 months. The clinical efficacy, scores of callus healing and joint function, and serum levels of calcium, phosphate, 1,5 dihydroxy vitamin D3 [1,5-(OH)2 D3], bone gla protein (BGP) and osteoprotegerin (OPG) were detected and compared between the groups. Results: ① The effective rate was 83.3% in the observation group and 59.5% in the control group, with a difference between the two groups (P<0.05). ② After treatment, the scores of callus healing, elbow and knee function in the observation group were higher than those of the control group (P<0.05, P<0.01). ③After treatment, the levels of calcium, phosphate in the serum, and levels of [1,5-(OH)2 D3], BGP and OPG were increased markedly in both groups (P<0.05, P<0.01), and the levels of observation group were higher than those of the control group (P<0.05). Conclusion: “Bushen Shengsui Decoction” can promote the fracture of extremities after operation, and its mechanisms may be association with improvement of microelements and promotion of BGP and OPG.关键词：limbs fracture;healing after surgery;“Bushen Shengsui Decoction”
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发布时间：2022-08-31 - 摘要：This paper analyzes Shi's “muscles and bones impediment” theory for the treatment of rheumatic disease. It is believed that simultaneous treatment of the muscles and bones, liver and kidney, qi and blood should be focused on, and eliminating external evil should be combined with for the clinical treatment of rheumatic disease. Different locations such as muscles and bones should take into account in specific patterns. Four proven cases are presented.关键词：rheumatic disease;Chinese medicine;Shi's department of traumatology;theory of “muscles and bones impediment”
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发布时间：2022-08-31 - 摘要：Deficiency of spleen and kidney being the root of renal albuminuria, turbid dampness, blood stasis and evil wind usually associated with it. This paper elaborates the common therapeutic methods such as invigorating spleen to remove dampness, benefiting kidney qi, clearing heat to dissolve dampness, securing and containing essence, activating blood circulation to eliminate stasis, dispersing wind etc. The medication is presented as well.关键词：albuminuria;kidney disease;deficiency of spleen and kidney
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发布时间：2022-08-31 - 摘要：This paper introduces Prof. Xu's academic thoughts on differentiation and treatment of lung cancer based on the theory of “treating both exterior and interior to inhibit the cancer”. Herbs with the function of harmonizing spleen and stomach to strengthen healthy qi, with the function of nourishing kidney essence to consolidate the root and cultivate origin. Medication should be based on disease differentiation, and mechanism of the disease should be considered to during the treatment. One proven case is presented.关键词：lung cancer;famous doctor's experience;Xu Zhenye
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发布时间：2022-08-31 - 摘要：This paper expounds the classification and effects of orifice-opening medicine. It is believed that seven orifices of the head and face are the gates of brain, which can be attacked by six evils. Thus, orifice diseases usually association with brain, which can be treated with orifice-opening medicine; diseases of brain and spirit can be treated by orifice-opening medicine.关键词：seven orifices;orifice disease;orifice-opening medicine
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发布时间：2022-08-31 - 摘要：Objective: On the basis of big data, this paper aims to discuss renal toxicity of the common herbs in department of rheumatology, such as Alismatis Rhizoma, Asari Radix et Rhizoma, Dutchmanspipe Herba Aristolochiae Mollissimae and Clematidis Radix et Rhizoma. Methods The personal data (gender, medicine dosage, frequency, etc.) of all patients of Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine taking Alismatis Rhizoma, Asari Radix et Rhizoma, Dutchmanspipe Herba Aristolochiae Mollissimae and Clematidis Radix et Rhizoma from January 1,9 to December 31,3 as stored in computers, prescriptions and renal function (creatinine, blood urea nitrogen, 24-hour urine protein) and other information total 12 560 000 were compared and sorted based on big data processing. Results: The renal dysfunction incidence of patients taking Alismatis Rhizoma, Asari Radix et Rhizoma, Dutchmanspipe Herba Aristolochiae Mollissimae and Clematidis Radix et Rhizoma was 1.15%, 2.46%, 1.50% and 0% respectively. Alismatis Rhizoma:Among patients taking Alismatis Rhizoma, proportion of those with renal dysfunction increased with the increase of cumulative dosage. Only one person clinically took Dutchmanspipe Herba Aristolochiae Mollissimae in recent five years, with cumulative dosage up to 300 g, and did not show renal dysfunction phenomenon. The renal dysfunction incidence was increased with the age of patients who took Alismatis Rhizoma, Asari Radix et Rhizoma and Clematidis Radix et Rhizoma. Conclusion: It is safe to use Alismatis Rhizoma, Asari Radix et Rhizoma and Clematidis Radix et Rhizoma by properly combining them under the guidance of traditional Chinese medicine theory. Renal dysfunction incidence is far less than that reported in relevant literature on experimental study.关键词：big data;Alismatis Rhizoma;Asari Radix et Rhizoma;Clematidis Radix et Rhizoma;renal toxicity
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发布时间：2022-08-31 - 摘要：Objective:To explore the similarities and differences of the metabonomics of yin deficiency liver-kidney pattern among kidney diseases, and to discuss the objective material basis of “ the same pattern in different diseases” on small molecules metabolites and relationship between disease and pattern. MethodsUltra performance liquid chromatography- mass spectrum (UPLC-MS) technology was adopted to test the blood metabolites on those with chronic kidney failure, chronic nephritis, chronic urinary tract infections and diabetic nephropathy of yin deficiency liver-kidney pattern. Meanwhile, partial-least-squares method was used to gain categorized statistics of the samples and difference variables by comparing that of healthy people. Common and unique difference variables were known by comparing of difference variables in each pathological group. The result was compared against the data in HMDB and Wiki Pathways database to identify those characteristic metabolites and corresponding metabolic pathways. Results:Patients with kidney diseases of yin deficiency liver-kidney pattern share four metabolites which are different from healthy people, namely 2-(hydroxyl diethanolamine)- Propionic acid, L- homocysteine, palmitic acid and Diguanosine tetraphosphate and 57 characteristic metabolites, among which 18 chronic kidney failure, 16 chronic nephritis, 14 chronic urinary tract infections and 9 diabetic nephropathy. Conclusion:The theory of “same pattern among different diseases” is objective and fact-based in chronic renal diseases. The same syndrome could be traced to the consistent material basis (biomarker), while the different diseases to different material basis. “Diseases” and “pattern” both have an impact on patients' metabolism, especially the “diseases”.关键词：chronic kidney disease;yin deficiency liver-kidney pattern;metabonomics;same pattern among different diseases
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发布时间：2022-08-31 - 关键词：Crohn's disease;dysentery;teaching ward round
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发布时间：2022-08-31 - 摘要：This paper summarizes Professor Xie Jianguo's experience in treating polycythemia vera. It is believed that branch of this disease is qi deficiency and blood stasis, and root of this disease is deficiency of kidney origin. Self-made formula “New Buyang Huanwu Decoction” can be used for the treatment of polycythemia vera. One proven case is presented.关键词：polycythemia vera;therapy of Chinese medicine;clinical experience;Xie Jianguo
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发布时间：2022-08-31 - 摘要：This paper introduces Yan Qianlin's clinical experience in treating cardiovascular disease with “Da Jianzhong Decoction” recorded in Lantai Guifan. It is believed that pathogen of cardiovascular disease in middle-aged people includes root defiency (deficiency of heart-yang) and branch excess (stagnation of phlegm and blood stasis). Benefiting qi and warming yang is used to treat the root; eliminating phlegm and dissolving phlegm is supplemented to treat the branch. Two proven cases are presented.关键词：cardiovascular disease;Xu's “Da Jianzhong Decoction”;famous doctor's experience;Yan Qianlin
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发布时间：2022-08-31 - 摘要：This paper introduces Professor Zhou Hua's experience with chronic heart failure (CHF) by the method of simultaneous treatment of the heart and kidney. It is believed that the causes of CHF are chronic deficiency of heart-yang and deficiency of kidney-yang. One proven case is presented.关键词：chronic heart failure (CHF);simultaneous treatment of the heart and kidney;Chinese medicine;famous doctor's experience;Zhou Hua
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发布时间：2022-08-31 - 摘要：This paper introduces Wang Zhongmin's clinical experience in treating the anovulatory dysfunctional uterine bleeding (DUB) by clearing source and obstructing flow method. It is viewed that DUB is characterized by amount of bleeding, long-time of bleeding, and emergency and crisis are easier to cause. Clearing source and obstructing flow the key to the treatment by the methods of promoting blood circulation to remove blood stasis and regulating menstruation to stop bleeding can be used to control the bleeding and avoid its recurrence with the conbination of hormones.关键词：dysfunctional uterine bleeding;anovulation;clearing source and obstructing flow method;famous doctor's experience;Wang Zhongmin
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发布时间：2022-08-31 - 摘要：This paper introduces the texts about dosage and medication of “Chaihu Plus Mangxiao Decoction” and “Chaihu Plus Longgu Muli Decoction” in different versions. The evidence is found by comparing the Song version with others. It was recorded in the Song version that clinical application of prescriptions in Chaihu category, which is benefit for the academic research and development of the cold damage diseases.关键词：Treatise on Cold Damage Diseases;“Chaihu Plus Mangxiao Decoction”;“Chaihu Plus Longgu Muli Decoction”;methods of decoction and administration
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发布时间：2022-08-31 - 摘要：This paper aims to discuss the characteristics of differentiating and treating exogenous diseases by analyzing seven medical records in Guide to Clinical Practice with Medical Records. It is viewed that Ye Tianshi paid attention to constitution factor for the treatment of exogenous diseases. Treatment principles and prescriptions should be based on the exogenous evil and constitution in clinic.关键词：Guide to Clinical Practice with Medical Records;Ye Tianshi;medical record;constitution;exogenous disease
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发布时间：2022-08-31 - 关键词：psoriasis vulgaris;psoriasis;disharmony between spleen and stomach;“Wendan Decoction”;medical record;Qin Yulong
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发布时间：2022-08-31 - 摘要：Objective:To observe the effect of “Dakang Formula” on expressions of HGF, c-Met, EGFR and PCNA in rats with ulcerative colitis (UC). MethodsSD rats were randomized into five groups. Except for the blank control group, models were established others were by TNBS (2,4, 6 - three of nitrobenzene sulfonic acid) in the other groups. The models were divided into model group, “Dakang Formula” groups (high, low dose) and positive groups (SASP), and each group was treated with corresponding measures. Eexpressions of HGF, c-Met, EGFR and PCNA in colon tissues were detected by immunohistochemical staining quantitative analysis method (gray value). Results:There were significant differences in expressions of HGF, c-Met, EGFR and PCNA (P<0.05). Compared with the model group, expressions of HGF, c-Met, EGFR and PCNA in the high-dose “Dakang Formula” group and positive group were increased. Expressions of c-Met and EGFR in the low-dose “Dakang Formula” group were increased, with significant difference between the two groups (P<0.05). There were significant differences in expressions of HGF and EGFR between the high-dose and low-dose “Dakang Formula” groups (P<0.05). Conclusion:“Dakang Formula” can promote the repair of colonic mucosa, recover the epithelial barrier function, reduce mucosal epithelial contact intestinal cavity caused by various antigen persistent inflammation and alleviate inflammatory reaction, promote colonic mucosal epithelial cell proliferation in colonic mucosa by intervening expressions of HGF, c-Met and EGFR.关键词：“Dakang Formula” group;enema;ulcerative colitis (UC);animal experiment;hepatocyte growth factor (HGF);receptor of HGF;epidermal growth factor receptor (EGFR);proliferating cell nuclear antigen (PCNA)
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发布时间：2022-08-31 -
Effect of polydatin on hypertrophy of rat myocardial cells and proliferation of fibroblasts in vitro
摘要：Objective:To observe the effect of polydatin on hypertrophy of rat myocardial cells and proliferation of fibroblasts in vitro, and to explore its antioxidation effect. MethodsLeft ventricle of neonatal SD rats were selected and its myocardial cells and fibroblasts were isolated and cultured by differential attachment method. The cardiomycytes and fibroblasts were divided into 5 groups:normal control group, polydatin control group (10-4 mol/L), model group (10-6 mol/L ISO), and low concentration polydatin group (10-5 mol/L+ISO 10-6 mol/L) and high concentration polydatin group (10-4 mol/L +ISO 10-6 mol/L). Different drugs intervened for 2 days, and protein content of cardiomycytes was determined by Coomassie Brilliant Blue; activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were detected; proliferation of fibroblasts and concentration of SOD, GSH-Px and NO in cell culture medium were determined. Results:Compared with the normal control group, protein content of cardiomyocytes was increased in the model group, and activities of SOD and GSH-Px were reduced markedly (P<0.05); compared with the model group, the protein content in high concentration polydatin group was markedly reduced, while the activities of SOD and GSH-Px were significantly increased (P<0.05); GSH-Px activity of the high concentration polydatin group was superior to that of the low concentration polydatin group, with signficiant difference between the two groups (P<0.05). Compared with the normal control group, 10-6 mol/L ISO could induce fibroblast proliferation and reduce the NO content, and activities of SOD and GSH-Px (P<0.05); compared with the model group, proliferation of fibroblasts was inhibited in the high concentration polydatin group, and the NO content, and activities of SOD and GSH-Px were increased markedly (P<0.05). Conclusion: Polydatin shows antagonism on ISO-induced hypertrophy of neonatal cardiomyocytes and proliferation of fibroblasts, its mechanism effect may relate to antioxidant and the production of NO.关键词：polydatin;cardiomyocyte;fibroblast;proliferation;anti-oxidant- 5
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发布时间：2022-08-31 - 摘要：Objective: To observe the clinical efficacy of compound “Lingzhi Jianshen Decoction” on expressions of AGT and IGF-1 in rats with diabetic nephropathy (DN), and to explore its mechanism. Methods Rat models with DN were established by injection of streptozotocin (STZ) and divided into the model group, treatment group and blank group. Treatment group was treated with compound “Lingzhi Jianshen Decoction”, and the others were treated with the same dose of distilled water. After 8 weeks, the general condition and biochemical indexes were observed, and the renal tissues were observed by immunohistochemical and pathological section. Levels of AGT and IGF-1 were detected by ELISA. Results: ① Compared with the blank group, the body weight of the model group and treatment group were reduced markedly (P<0.01), and ratio of kidney wight to BMI, biochemical indexes, levels of AGT and IGF-1 were increased significantly (P<0.01). Pathological examination of kidney in rats showed glomerular hypertrophy, mesangial matrix proliferation, glomerular basement membrane thickening, tubular epithelial cell degeneration, protein cast formation in some renal tubules. The levels of AGT and IGF-1 in the kikdney were markedly increased. ② Compared with the model group, the body weight of the treatment group was remarkably increased (P<0.01), while the levels of 24 albuminuria, BUN, SCr and AGT and IGF-1 were reduced markedly, with significant difference between the two groups (P<0.05). The pathologic changes were much more alleviated in the treatment group than that of the modle group. Conclusion: Compound “Lingzhi Jianshen Decoction” can protect the kidney function of diabetic nephropathy rats. Its mechanism may be association with the reducing expression of AGT and IGF-1 and inhibting activity of RAS.关键词：diabetic nephropathy (DN);compound “Lingzhi Jianshen Decoction”;AGT;IGF-1
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发布时间：2022-08-31 - 摘要：Objective:To observe the effect of laminarin on survival and apoptosis of human hepatocarcinoma cell of Bel-7402.Methods① Cultured human hepatocarcinoma cell of Bel-7402 in vitro, after added with the 5,0, 20,0, 40 g/L laminarin, cultured for 48 h. Without adding laminarin as the control group, we observed the changes of cells by inverted microscope and detected the cell viability by MTT assay. ② Cultured humanhepatocarcinoma cell of Bel-7402 in vitro, three groups:The control group, laminarin group and laminarin+ medicine group were treated with 10,0 and 30 g/L respectively. The cell cycle and apoptosis rate were analyzed by flow cytometer. Results:① After bel-7402 cells were cultured using 5,0, 20,0, 40 g/L laminarin for 48 h, with the increasement of laminarin concentration, bel-7402 cells were shrinked and changes. The cell survival rate was significantly decreased. ② After 48h, the bel-7402 cells apoptosis rate was (14.8 ±1.7)% and death rate was (19.8±2.3), with significant difference from the control group (P＜0.05). Conclusion:Laminarin can inhibit survival rate, and promote the apoptosis and necrocytosis of bel-7402 cells.关键词：laminarin;bel-7402 cell;apoptosis;cell cycle
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发布时间：2022-08-31 - 摘要：Objective:To discuss the effect of ginseng and scutellaria bionic extract (SQBE) on HIF-1α expression in HaCaT cells irradiated by UVB and to expound the possible mechanism of anti-UVB radiation on skin cancer. MethodsLogarithmic growth phase HaCaT cells were seeded in petri dishes containing 10% fetal bovine serum in DMEM medium, and cultured in 37 ℃, 5% CO2 incubator; We divided into a control group, SQBE 1 group (12.5 μg·ml-1 SQBE), SQBE 2 group (25.0 μg·ml-1 SQBE), and SQBE 3 group (50.0 μg·ml-1 SQBE), each group was irradiated by UVB (0,0, 40,0 mJ·cm-2) 4 hours after administration. After 24 hours, the cell survival rate was detected by MTT method; expression of HIF-1αmRNA and HIF-1α protein were detected by real-time PCR and Western blotting. Results:Compared with the control group at the same dose, the cell survival rate in the SQBE 3 group was increased statistically (P<0.05), and no significant difference was found between SQBE 1 group and SQBE 2 group (P>0.05); the expressions of HIF-1αmRNA and HIF-1α protein were decreased markedly in the SQBE3 group. Conclusion:SQBE can prevent the UVB-induced skin cancer by influcing the expression of HIF-1α in cells possibly.关键词：ginseng;scutellaria;HaCaT cell;UVB;hypoxia-inducible factor 1α (HIF-1α)
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发布时间：2022-08-31 - 摘要：Objective: To observe the anti-thrombus function of ginkgolide B drug eluting stent in models. Methods ① Detection of anticoagulation effect in vitro:2 ml sodium citrate anticoagulation was dripped into tubes. We will take ginkgolide B and 316L bare metal stents for comparison. The APTT, PT, and TT were detected in each group for 3 times. ② Arteriovenous shunt models:a healthy pig was used to establish the model and divided into two groups:ginkgolide B stent group and 316L bare metal stent group. The thrombosis in each group was observed by electric magnifier. ③ Model of high load thrombosis:two healthy pigs were used to establish the models and divided into ginkgolide B stent group and 316L bare metal stent group, each group was treated with corresponding measures. The time of thrombosis in each group was observed. Results: ① APTT time and TT time were significantly prolonged in ginkgolide B coating group in vitro. ② Results in arteriovenous shunt thrombosis models showed that the weight of stent thrombosis was lighter in Ginkgolide B drug eluting stent compared with 316 L bare metal. ③ Results in high load thrombosis models showed that the ginkgolide B drug eluting stent in an average of (140.7±12.3) seconds stent thrombosis, and corresponding 316L stents an average of (52.7±6) seconds stent thrombosis, with a statistical difference between the two groups (P<0.05). Conclusion: Ginkgolide B drug eluting stent has good anti thrombosis effect, which is expected to be a coating drug in eluting stent selection.
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发布时间：2022-08-31 - 摘要：This paper reviews the research progress on experimental models of non-alcoholic fatty liver disease (NAFLD) in recent years. It views that animal models mainly include eating disorder-induced model, L-022 model and HepG2 model.关键词：fatty liver;experimental model;review
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发布时间：2022-08-31
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