Isolation and biological activities study of five alkaloid compounds from <italic style="font-style: italic">Cephalotaxus fortunei</italic>

CHEN Haoyuan; QIN Jiaxu; YAN Hongling; LI Haiyang; ZHAO Qunfei; HE Qingli

doi:10.16305/j.1007-1334.2025.z20250422001

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Isolation and biological activities study of five alkaloid compounds from Cephalotaxus fortunei

更新时间：2025-12-12

- Isolation and biological activities study of five alkaloid compounds from Cephalotaxus fortunei
- Shanghai Journal of Traditional Chinese Medicine Vol. 59, Issue 12, Pages: 76-82(2025)
- 作者机构：
  
  上海中医药大学创新中药研究院，中药功效成分发掘与利用全国重点实验室（上海 201203）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2025.z20250422001
  CLC：
- Received：22 April 2025，
  
  Published：10 December 2025
- 稿件说明：
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陈昊元,秦加旭,闫洪玲,等.三尖杉中5个生物碱类化合物的分离和生物活性研究[J].上海中医药杂志,2025,59(12):76-82.

CHEN Haoyuan,QIN Jiaxu,YAN Hongling,et al.Isolation and biological activities study of five alkaloid compounds from Cephalotaxus fortunei[J].Shanghai Journal of Traditional Chinese Medicine,2025,59(12):76-82.
陈昊元,秦加旭,闫洪玲,等.三尖杉中5个生物碱类化合物的分离和生物活性研究[J].上海中医药杂志,2025,59(12):76-82. DOI： 10.16305/j.1007-1334.2025.z20250422001.

CHEN Haoyuan,QIN Jiaxu,YAN Hongling,et al.Isolation and biological activities study of five alkaloid compounds from Cephalotaxus fortunei[J].Shanghai Journal of Traditional Chinese Medicine,2025,59(12):76-82. DOI： 10.16305/j.1007-1334.2025.z20250422001.

摘要

目的

发掘三尖杉的生物碱类成分，并探索其生物活性。

方法

利用硅胶色谱、葡聚糖凝胶LH-20色谱及半制备高效液相色谱等多种色谱技术，从三尖杉二氯甲烷部位分离生物碱类化合物；利用一维/二维核磁共振（1D/2D NMR）、高分辨电喷雾电离质谱（HR-ESI-MS）和电子圆二色谱（ECD）分析确定化合物结构；利用刃天青（Alamar Blue）法检测化合物对宫颈癌细胞HeLa的细胞毒性，以及化合物对过氧化氢（H

）诱导的人角质形成细胞（HaCaT）氧化损伤的保护作用。

结果

从三尖杉中共分离得到5个生物碱类化合物Cephalocyclidin A-

-oxide（1）、（

）-Fortunine（2）、4-Hydroxycephalotaxine（3）、Cephalocyclidin A（4）和Wilsonine（5），其中1为新化合物。体外活性检测显示，生物碱类化合物1~5没有明显的抗宫颈癌活性，其对HeLa细胞的半数抑制浓度（IC

）均

100 μmol/L。化合物1~5对H

诱导的HaCaT氧化损伤均具有显著的保护作用（

0.01），且能抑制H

刺激引起的活性氧（ROS）水平升高，其中化合物2、4和5的差异具有统计学意义（

0.05）。

结论

从三尖杉中分离得到的5种无侧链的生物碱类化合物对宫颈癌细胞无明显的抑制作用，但对H₂O₂诱导的HaCaT氧化损伤具有显著的保护作用。这些发现为三尖杉来源天然产物的功效研究提供了新的方向。

Abstract

Objective

To investigate the alkaloid components of

Cephalotaxus fortunei

and explore their biological activities.

Methods

Alkaloids compounds were isolated from the dichloromethane fraction of

Cephalotaxus fortunei

using chromatographic techniques such as silica gel， glucan gel LH-20， and semi-preparative high-performance liquid chromatography. The structures of the compounds were elucidated via one-dimensional/two-dimensional nuclear magnetic resonance （1D/2D NMR）， high-resolution electrospray ionization mass spectrometry （HR-ESI-MS）， and electronic circular dichroism （ECD） analyses. The cytotoxic effect against HeLa cervical cancer cells and protective activity against H

-induced oxidative damage in Human keratinocytes （HaCaT） were assessed using the Alamar Blue assay.

Results

Five alkaloids compounds Cephalocyclidin A-

-oxide （1），（

）-Fortunine （2）， 4-Hydroxycephalotaxine （3）， Cephalocyclidin A （4）， and Wilsonine （5） were isolated， of which compounds 1 is a new compound.

In vitro

activity assays showed that alkaloid compounds 1~5 had no significant anti-cervical cancer activity， and the median inhibitory concentration （IC

） values for these compounds against HeLa cells were all

100 μmol/L. Compounds 1~5 all exhibited significant protective effects against H

-induced oxidative damage in HaCaT （

0.01）， and were able to inhibit the increase of reactive oxygen （ROS） levels caused by H

stimulation， among which the differences of compounds 2， 4 and 5 were statistically significant （

0.05）.

Conclusions

The five alkaloid compounds without side chains isolated from

Cephalotaxus fortunei

had no significant inhibitory effect on cervical cancer cells. However， they demonstrated notable protective activity against H₂O₂-induced oxidative damage in HaCaT. These findings suggest a new direction for the efficacy study of natural products derived from

Cephalotaxus fortunei

关键词

Keywords

references

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Isolation and biological activities study of five alkaloid compounds from Cephalotaxus fortunei

DOI：10.16305/j.1007-1334.2025.z20250422001

摘要

Abstract

关键词

Keywords

references