Objective,2,To investigate the mechanism of Chaihu Shugan Powder in treating functional dyspepsia （FD） in rats through exploring oxidative stress and the AMP-activated protein kinase/sirtuin 3（AMPK/SIRT3） signaling pathway.,Methods,2,Thirty-two SD rats were randomly divided into four groups： normal group， model group， Chaihu Shugan Powder group， and domperidone group， with eight rats in each group. The model group， Chaihu Shugan Powder group， and domperidone group were modeled using the modified tail-clamping stimulation method. The normal group and model group were administered 0.9% sodium chloride solution by gavage， while the Chaihu Shugan Powder group and domperidone group were administered Chaihu Shugan Powder aqueous decoction （4.8 g/kg） and domperidone suspension （4.5 mg/kg） by gavage for four consecutive weeks. After the intervention， the gastric emptying rate of the rats was detected， hematoxylin-eosin （HE） staining was used to observe the histopathological changes in the gastric tissue， transmission electron microscopy was used to observe the mitochondrial ultrastructure of gastric interstitial cells （ICCs）， and enzyme-linked immunosorbent assay （ELISA） was used to detect the levels of reactive oxygen species （ROS）， malondialdehyde （MDA）， catalase （CAT）， superoxide dismutase （SOD）， and glutathione peroxidase （GSH-Px） in the rat serum. The mRNA expression levels of ,AMPK,， ,SIRT3,， and manganese superoxide dismutase （,MnSOD,） in the rat gastric tissue were quantified using real-time fluorescence quantitative reverse transcription-polymerase chain reaction （RT-qPCR）. Additionally， Western blot assay was used to detect the protein expression levels of phosphorylated AMPK （p-AMPK）， AMPK， SIRT3， MnSOD， microtubule-associated protein light chain 3 （LC3）， and P62 in the rat gastric tissue.,Results,2,①The model group of rats exhibited a lower gastric emptying rate than the normal group （,P,<,0.05）. Additionally， the mitochondria of ICCs in the model group displayed characteristics such as swelling， irregular morphology， increased branching， vacuolization， and the presence of autophagic vesicles. The gastric emptying rate was higher in both the Chaihu Shugan Powder group and the domperidone group than that in the model group （,P,<,0.05）， and there was a reduction in damage to the ICCs mitochondrial structure in both groups. ②Compared with the normal group， the model group showed elevated serum levels of ROS and MDA （,P,<,0.05） and decreased levels of SOD， CAT， and GSH-Px （,P,<,0.05）. However， compared with the model group， the Chaihu Shugan Powder group and the domperidone group exhibited decreased serum levels of ROS and MDA （,P,<,0.05） and increased levels of SOD， CAT， and GSH-Px （,P,<,0.05）. ③The mRNA expression levels of ,AMPK,， ,SIRT3,， and ,MnSOD ,in the gastric tissues of rats were reduced in the model group compared with those levels in the normal group （,P,<,0.05）. However， both the Chaihu Shugan Powder group and the domperidone group showed higher mRNA expression levels of ,AMPK,， ,SIRT3,， and ,MnSOD, in the rat gastric tissues than the model group （,P,<,0.05）. ④The protein expression level of LC3Ⅱ in the rat gastric tissues was higher in the model group than that in the normal group （,P,<,0.05）. Furthermore， the protein expression levels of SIRT3， MnSOD， P62， and the ratio of p-AMPK/AMPK were lowered （,P,<,0.05）. Conversely， the protein expression level of LC3Ⅱ in the rat gastric tissues was lower in both the Chaihu Shugan Powder group and the domperidone group than that in the model group （,P,<,0.05）， and the protein expression levels of SIRT3， MnSOD， P62， and the ratio of p-AMPK/AMPK were increased in both the Chaihu Shugan Powder group and the domperidone group （,P,<,0.05）.,Conclusion,2,Chaihu Shugan Powder can effectively promote gastric motility in rats with FD by activating the AMPK/SIRT3 pathway， reducing mitochondrial oxidative stress， and inhibiting autophagy in ICCs.