WANG Xiaoyi,QIU Kun,CHEN Ming,et al.Quantitative analysis of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 by HPLC and their intestinal mucosal permeability[J].Shanghai Journal of Traditional Chinese Medicine,2023,57(9):78-84.
WANG Xiaoyi,QIU Kun,CHEN Ming,et al.Quantitative analysis of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 by HPLC and their intestinal mucosal permeability[J].Shanghai Journal of Traditional Chinese Medicine,2023,57(9):78-84. DOI: 10.16305/j.1007-1334.2023.2306001.
Quantitative analysis of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 by HPLC and their intestinal mucosal permeability
Objective,2,To investigate the effects of notoginsenoside R1, ginsenoside Rg1 and Rb1 on intestinal mucus permeation by using three ,in vitro, intestinal mucus permeation models[purified mucin infiltration model (PIM), artificial intestinal mucus infiltration model (AIM) and rat intestinal mucus infiltration model (RIM)] in order to provide experimental basis for the oral absorption and application of notoginseng Radix Et Rhizoma.,Methods,2,Quantitative analysis methods of notoginsenoside R1, ginsenoside Rg1 and Rb1 were established by high performance liquid chromatography (HPLC), and their linear range, precision, stability, reproducibility and sample recovery rate were investigated. The apparent permeability coefficients (Papp) of different concentrations of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 solutions on three intestinal mucus permeation models were determined and analyzed to compare their intestinal mucus permeation effects.,Results,2,The HPLC methods for the quantitative analysis of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 were successfully established. The precision, stability, reproducibility and recovery all met the requirements. The permeability results of three kinds of saponins in PIM, AIM and RIM models showed that the permeability of notoginsenoside R1 and ginsenoside Rg1 and ginsenoside Rb1 in the three models increased in different degrees with the increase of drug concentration. In PIM, notoginsenoside R1 showed a significant increase in the permeation rate compared with ginsenoside Rb1 and ginsenoside Rg1 at different concentrations (,P,<,0.05). In AIM, the permeation rate at a concentration of 20 g,·,L,-1, was Papp,R1,>, Papp,Rb1,>, Papp,Rg1, (,P,<,0.05). In RIM, the permeation rate at a concentration of 20 g,·,L,-1 ,was Papp,Rg1,>, Papp,R1,>, Papp,Rb1, (,P,<,0.05).,Conclusions,2,Notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 with different concentrations have good stability and reproducibility in three intestinal mucus permeation models, and the permeability increases with the increase of drug concentration. Notoginsenoside R1 with strong water solubility and relatively low molecular weight is more permeable in PIM and AIM with less lipids, while ginsenoside Rg1 with strong fat solubility is stronger in RIM.
关键词
三七三七皂苷R1人参皂苷Rg1人参皂苷Rb1肠吸收生物利用度中药研究
Keywords
notoginseng Radix Et Rhizomnotoginsenoside R1ginsenoside Rg1ginsenoside Rb1intestinal absorptionbioavailabilitytraditional Chinese herbal medicine research
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