Identification and functional characterization of a bufalin 7β⁃hydroxylase from <italic style="font-style: italic">Fusarium fujikuroi</italic>

ZHONG Xueqing; ZHAO Qunfei

doi:10.16305/j.1007-1334.2023.2305049

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Identification and functional characterization of a bufalin 7β⁃hydroxylase from Fusarium fujikuroi

更新时间：2023-08-28

- Identification and functional characterization of a bufalin 7β⁃hydroxylase from Fusarium fujikuroi
- Shanghai Journal of Traditional Chinese Medicine Vol. 57, Issue 9, Pages: 19-26(2023)
- 作者机构：
  
  1.上海中医药大学创新中药研究院手性药物研究中心（上海 201203）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2023.2305049
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钟雪庆,赵群飞.藤仓赤霉菌中蟾毒灵7β⁃羟化酶的发现与功能鉴定[J].上海中医药杂志,2023,57(9):19-26.

ZHONG Xueqing,ZHAO Qunfei.Identification and functional characterization of a bufalin 7β⁃hydroxylase from Fusarium fujikuroi[J].Shanghai Journal of Traditional Chinese Medicine,2023,57(9):19-26.
钟雪庆,赵群飞.藤仓赤霉菌中蟾毒灵7β⁃羟化酶的发现与功能鉴定[J].上海中医药杂志,2023,57(9):19-26. DOI： 10.16305/j.1007-1334.2023.2305049.

ZHONG Xueqing,ZHAO Qunfei.Identification and functional characterization of a bufalin 7β⁃hydroxylase from Fusarium fujikuroi[J].Shanghai Journal of Traditional Chinese Medicine,2023,57(9):19-26. DOI： 10.16305/j.1007-1334.2023.2305049.

摘要

目的,2,挖掘能催化蟾蜍内酯类化合物特异性羟化修饰的酶，以促进名贵中药材蟾酥功效组分的开发与应用。,方法,2,采用同源序列比对挖掘藤仓赤霉菌（,Fusarium fujikuroi,）中的甾体羟化酶，通过毕赤酵母异源表达、工程菌株细胞转化以及代谢产物的分离与鉴定，从而确定酶的功能。,结果,2,以蟾蜍内酯类化合物蟾毒它灵、蟾毒灵为底物，毕赤酵母重组菌株KM71H-,FF294,转化蟾毒它灵没有产生明显的产物，而转化蟾毒灵时产生了羟化产物7β-羟基蟾毒灵。,结论,2,从藤仓赤霉菌中发现能催化蟾毒灵发生7β-羟化修饰的甾体羟化酶FF294，是目前文献报道首个能催化蟾蜍内酯类化合物羟基化反应的酶。

Abstract

Objective,2,To explore enzymes that catalyze specific hydroxylation modification of bufadienolides so as to promote the development and application of the efficacy components of the rare traditional Chinese medicine Chansu （Bufonis Venenum）.,Methods,2,The steroid hydroxylase from ,Fusarium fujikuroi ,was discovered by sequence alignment， and its function was determined by heterologous expression in ,Pichia pastoris ,and isolation and identification of the metabolites transformed by the engineered strains.,Results,2,The activity of the strain KM71H-,FF294, was investigated by using bufalin and bufotaline as substrates. No product was produced when KM71H-,FF294, transformed bufotaline， but the hydroxylation product 7β-OH bufalin was produced when bufalin was transformed.,Conclusion,2,Steroid hydroxylase FF294 from ,Fusarium fujikuroi, could catalyze the conversion of bufalin into 7β-hydroxylated products， and this is the first report of an enzyme that catalyzes the hydroxylation of bufadienolides.

关键词

蟾酥蟾蜍内酯类化合物羟化酶结构修饰活性成分中药研究

Keywords

Chansu （Bufonis Venenum）bufadienolidessteroid hydroxylasestructural modificationactive componenttraditional Chinese medicine research

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Identification and functional characterization of a bufalin 7β⁃hydroxylase from Fusarium fujikuroi

DOI：10.16305/j.1007-1334.2023.2305049

摘要

Abstract

关键词

Keywords

references