Chemical profiling of Huangqi Decoction granules and characterization of absorbed components and metabolites in mice plasma following oral administration based on UHPLC⁃Q⁃Exactive Orbitrap HRMS

ZHANG Qing; LI Rongsheng; HUANG Sihong; SHEN Qin; YIN Yixiao; JIANG Shili; MU Yongping; CHEN Jiamei; LIU Ping; LIU Wei

doi:10.16305/j.1007-1334.2023.2303062

您当前的位置：

首页 >

文章列表页 >

Chemical profiling of Huangqi Decoction granules and characterization of absorbed components and metabolites in mice plasma following oral administration based on UHPLC⁃Q⁃Exactive Orbitrap HRMS

更新时间：2023-08-28

- Chemical profiling of Huangqi Decoction granules and characterization of absorbed components and metabolites in mice plasma following oral administration based on UHPLC⁃Q⁃Exactive Orbitrap HRMS
- Shanghai Journal of Traditional Chinese Medicine Vol. 57, Issue 9, Pages: 70-77(2023)
- 作者机构：
  
  1.上海中医药大学附属曙光医院肝病研究所，肝肾疾病病证教育部重点实验室，上海市中医临床重点实验室（上海 201203）
  2.上海中医药大学附属曙光医院药学部国家中医药管理局中药制剂三级实验室（上海 201203）
  3.上海中医药大学附属曙光医院宝山分院儿科（上海 201900）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2023.2303062
  CLC：
扫描看全文
张清,李荣胜,黄思红,等.基于UHPLC⁃Q⁃Exactive Orbitrap HRMS技术分析黄芪汤颗粒的化学成分与小鼠口服后的入血成分[J].上海中医药杂志,2023,57(9):70-77.

ZHANG Qing,LI Rongsheng,HUANG Sihong,et al.Chemical profiling of Huangqi Decoction granules and characterization of absorbed components and metabolites in mice plasma following oral administration based on UHPLC⁃Q⁃Exactive Orbitrap HRMS[J].Shanghai Journal of Traditional Chinese Medicine,2023,57(9):70-77.
张清,李荣胜,黄思红,等.基于UHPLC⁃Q⁃Exactive Orbitrap HRMS技术分析黄芪汤颗粒的化学成分与小鼠口服后的入血成分[J].上海中医药杂志,2023,57(9):70-77. DOI： 10.16305/j.1007-1334.2023.2303062.

ZHANG Qing,LI Rongsheng,HUANG Sihong,et al.Chemical profiling of Huangqi Decoction granules and characterization of absorbed components and metabolites in mice plasma following oral administration based on UHPLC⁃Q⁃Exactive Orbitrap HRMS[J].Shanghai Journal of Traditional Chinese Medicine,2023,57(9):70-77. DOI： 10.16305/j.1007-1334.2023.2303062.

摘要

目的,2,运用超高效液相串联四极杆-静电场轨道阱高分辨质谱（UHPLC-Q-Exactive Orbitrap HRMS）技术，分析鉴定黄芪汤颗粒的化学成分及小鼠灌胃黄芪汤颗粒后的主要入血成分。,方法,2,色谱分离采用Acquity UPLC BEH C,18,色谱柱（100 mm×2.1 mm，1.7 µm），以甲醇-0.1%甲酸水溶液为流动相梯度洗脱。质谱采用电喷雾电离正离子模式（ESI,+,）和电喷雾电离负离子模式（ESI,-,）交替扫描，并自动触发二级质谱扫描功能（Full MS/dd-MS,2,）采集质谱数据。经Xcalibur 3.0软件获取色谱保留时间以及母离子和碎片离子信息，定性鉴别黄芪汤颗粒的化学成分及小鼠灌胃黄芪汤颗粒后的入血成分及可能的代谢途径。,结果,2,综合保留时间、准确分子量、二级碎片信息、对照品比对、文献参考等色谱质谱检测信息，从黄芪汤颗粒中共鉴定出167种化学成分，从小鼠灌胃黄芪汤颗粒后的血清中共检测到甘草素、毛蕊异黄酮、芒柄花素、黄芪甲苷等60种原型成分和43种代谢产物。,结论,2,完成了黄芪汤颗粒化学成分及主要入血成分快速鉴定，可为黄芪汤颗粒效应物质解析、质量标准研究及作用机制探讨等提供参考。

Abstract

Objective,2,To analyze and identify the chemical composition and the main absorbed components in mice after oral administration of Huangqi Decoction granules by UHPLC-Q-Exactive Orbitrap HRMS technique.,Methods,2,Chromatographic separation was performed on an Acquity UPLC BEH C,18, column （100 mm×2.1 mm， 1.7 µm）， and 0.1% formic acid aqueous solution-methanol as the mobile phase with gradient elution. The mass spectra were collected using electrospray ionization source ESI,+, and ESI,- ,modes with alternating scans and automatic triggering of the secondary mass spectrometry scan function （Full MS/dd-MS,2,）. The chemical composition in Huangqi Decoction granules， absorbed components and metabolites in the serum of mice after oral administration of Huangqi Decoction granules were identified by Xcalibur 3.0 software and compared with reference standards， literatures， and database.,Results,2,A total of 167 chemical compositions were identified from Huangqi Decoction granules， and 60 prototypical components and 43 metabolites were detected in the serum， including liquiritigenin， calycosin， formononetin and astragaloside Ⅳ， etc.,Conclusion,2,This study successfully completed the rapid identification of chemical composition from Huangqi Decoction granules， and clarified main blood components after treated with Huangqi Decoction granules， which can provide partial reference for the analysis of effective substances， study of quality control and explore of action mechanism of Huangqi Decoction granules.

关键词

黄芪汤慢性肝病经典名方化学成分代谢产物入血成分

Keywords

Huangqi Decoctionchronic liver diseaseChinese classic formulaschemical compositionmetabolitesabsorbed components

references

赵丽珠，杨波，孙向明，等. 黄芪汤化学成分分析及其治疗非酒精性脂肪肝病的机制［J］. 药学学报，2021， 56（11）： 3082-3090.

王浩艺，成扬. 黄芪汤及方中单味药研究进展［J］. 上海中医药杂志，2021， 55（8）： 99-103.

张具斌，朴成玉，刘婷婷，等.中药调控NF⁃κB相关信号通路防治糖尿病肾病的研究进展［J］.上海中医药杂志，2022， 56（5）： 98-101.

蒋微，徐莹，王晓柠，等. 黄芪汤上调肝组织BAMBI表达改善CCl4诱导小鼠肝纤维化［J］. 上海中医药杂志，2021， 55（3）： 74-80.

陈高峰，麦静愔，陈澍，等. 黄芪汤对肝硬化大鼠胶原沉积和肝窦毛细血管化标志物表达的影响［J］. 现代中西医结合杂志，2022， 31（16）： 2205-2210.

LIU W， HUANG J， ZHANG F， et al. Comprehensive profiling and characterization of the absorbed components and metabolites in mice serum and tissues following oral administration of Qing-Fei-Pai-Du decoction by UHPLC-Q-Exactive-Orbitrap HRMS［J］. Chin J Nat Med， 2021， 19（4）： 305-320.

王永丽，张聪聪，张凤，等. UHPLC-Q-Exactive Orbitrap HR-MS分析荆银颗粒的化学成分及其组织分布特征［J］. 中国中药杂志，2020， 45（22）： 5537-5554.

王立会，张娜，俞磊明，等. 基于UPLC-QTOF-MSE植物代谢组学的不同产地黄芪差异性研究［J］. 中国药师，2022， 25（12）： 2165-2170.

胡妮娜，张晓娟. 黄芪的化学成分及药理作用研究进展［J］. 中医药信息，2021， 38（1）： 76-82.

ZHANG L，HU Y，QI S，et al. Astragalus saponins and its main constituents ameliorate ductular reaction and liver fibrosis in a mouse model of DDC-induced cholestatic liver disease［J］. Front Pharmacol， 2022， 13： 965914.

王永丽，黄广建，刘从进，等. UHPLC-Q-Exactive Orbitrap HRMS分析黄连解毒汤的化学成分及大鼠组织分布［J］. 中草药，2022， 53（22）： 6985-7000.

乔湜，许海山，石晓伟，等. UHPLC-QTOF-MS法分析鉴定止咳宝片在大鼠血浆中的代谢产物［J］. 药学学报，2018， 53（9）： 1536-1544.

刘开心，祁永华，张喜武，等. 基于UPLC-Q-TOF-MS技术的圣愈汤干预骨髓抑制大鼠的体内显效成分分析［J］. 中药药理与临床，2022， 38（2）： 7-13.

李玲，江美芳，赵林松，等. 林下山参、园参、红参与西洋参粉末的化学成分及快速无损鉴别［J］. 上海中医药杂志，2023， 57（1）： 76-86.

LI W K， WANG G F， WANG T M， et al. Protective effect of herbal medicine Huangqi decoction against chronic cholestatic liver injury by inhibiting bile acid-stimulated inflammation in DDC-induced mice［J］. Phytomedicine， 2019， 62： 152948.

DU J X，SUN M Y，DU G L，et al. Ingredients of Huangqi decoction slow biliary fibrosis progression by inhibiting the activation of the transforming growth factor-beta signaling pathway［J］. BMC Complement Altern Med， 2012， 12： 33.

ZHOU Y， TONG X， REN S， et al. Synergistic anti-liver fibrosis actions of total astragalus saponins and glycyrrhizic acid via TGF-β1/Smads signaling pathway modulation［J］. J Ethnopharmacol， 2016， 190： 83-90.

MU Y P， XIAO Z， LI X W， et al. Astragaloside prevents BDL-induced liver fibrosis through inhibition of notch signaling activation［J］. J Ethnopharmacol， 2015， 169： 200-209.

Views

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mechanism of Huangqi Decoction against liver fibrosis by network pharmacological analysis combined with related cell experiment

Shi Weiqun's experience in treatment of hepatic encephalopathy with Shengjiang Powder based on theory of “release of fire depression”

Research progress on bile acid metabolism and its receptors regulating intestinal immunity in ulcerative colitis and traditional Chinese herbal medicine intervention

New explanation of Dahuang Gansui Decoction article in Synopsis of Golden Chamber

Effect of Modified Xuanbai Chengqi Decoction on intestinal flora and immunity of mice with mycoplasma pneumoniae pneumonia

Related Author

No data

Related Institution

School of Traditional Chinese Medicine， Shanghai University of Traditional Chinese Medicine

Institute of Interdisciplinary Integrative Medicine Research， Shanghai University of Traditional Chinese Medicine

Shi Weiqun National Famous Traditional Chinese Medicine Expert Inheritance Studio

The Second Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine

Hangzhou Xixi Hospital Affiliated to Zhejiang University School of Medicine

⁰