ZHANG Qian,KIM Mooseob,LU Meilong,et al.Study on characteristic chromatograms and uridine content determination ofAcori Tatarinowii Rhizoma formula granules[J].Shanghai Journal of Traditional Chinese Medicine,2023,57(7):72-80.
ZHANG Qian,KIM Mooseob,LU Meilong,et al.Study on characteristic chromatograms and uridine content determination ofAcori Tatarinowii Rhizoma formula granules[J].Shanghai Journal of Traditional Chinese Medicine,2023,57(7):72-80. DOI: 10.16305/j.1007-1334.2023.2211143.
Study on characteristic chromatograms and uridine content determination ofAcori Tatarinowii Rhizoma formula granules
Objective,2,To control the internal quality of Shichangpu (Acori Tatarinowii Rhizoma) formula granules, the overall characteristic chromatogram by ultra-performance liquid chromatography (UPLC) and the characteristic chromatogram of water-soluble components by high-performance liquid chromatography (HPLC) were established, and the content of uridine, a nucleic acid component, was determined by HPLC.,Methods,2,The overall UPLC characteristic chromatogram was determined on a Waters ACQUITY UPLC BEH C,18, column (2.1 mm×100 mm, 1.7 μm) with a gradient elution of methanol and 0.1% phosphoric acid aqueous solution as mobile phases at a flow rate of 0.4 mL/min, detection wavelength of 280 nm, column temperature of 35 ℃, and injection volume of 1 μL. The HPLC characteristic chromatogram of water-soluble components and content determination were performed on a SHIMADZU Shim-pack GIST C,18,-AQ column (4.6 mm×250 mm, 5 μm) with a gradient elution of methanol and 0.1% phosphoric acid aqueous solution as mobile phases at a flow rate of 1 mL/min, detection wavelength of 260 nm, column temperature of 35 ℃, and injection volume of 10 μL.,Results,2,The overall characteristic chromatogram of Acori Tatarinowii Rhizoma formula granules was established with 9 characteristic peaks, among which 4 peaks were identified as asarylaldehyde (peak 3), ,N,-,trans,-feruloyltyramine (peak 4), ,β,-asarone (peak 6), and ,α,-asarone (peak 9), with similarities of 10 bathes of samples ranging from 0.994 to 1.000. The water solubility characteristic chromatogram Acori Tatarinowii Rhizoma formula granules was established with 8 characteristic peaks, among which 5 component peaks were identified as adenine (peak 1), uridine (peak 2), adenosine (peak 3), guanosine (peak 5), and ,L,-phenylalanine (peak 7), with similarities of 23 batches of samples ranging from 0.931 and 0.972. According to the quantitative analysis, the concentration of uridine had a good linear relationship with the peak area in the linear range of 2.086 to 104.300 mg/L (,R,2,=1.000 0), the mean spiked recovery was 100.16%, the ,RSD, value was 2.02%, and the uridine content of 23 batches of Acori Tatarinowii Rhizoma formula granules ranged from 0.037% to 0.061%, showing the stable quality of the formulation granules.,Conclusion,2,The established characteristic chromatograms and content determination methods are simple, reproducible, and specific, and can be used for the quality control of Acori Tatarinowii Rhizoma formula granules.
关键词
石菖蒲配方颗粒特征图谱含量测定高效液相色谱质量标准中药研究
Keywords
Shichangpu (Acori Tatarinowii Rhizoma)formula granulecharacteristic chromatogramassayHPLCquality standardtraditional Chinese herbal medicine research
references
GENG Y, LI C, LIU J, et al. Beta-asarone improves cognitive function by suppressing neuronal apoptosis in the beta-amyloid hippocampus injection rats[J]. Biol Pharm Bull, 2010, 33(5): 836-843.
ZHU M, ZHU H, TAN N, et al. The effects of Acorus tatarinowii Schott on 5-HT concentrations, TPH2 and 5-HT1B expression in the dorsal raphe of exercised rats[J]. J Ethnopharmacol, 2014, 158: 431-436.
MO Z T, FANG Y Q, HE Y P, et al. β-Asarone protects PC12 cells against OGD/R-induced injury via attenuating Beclin-1-dependent autophagy[J]. Acta Pharmacol Sin, 2012, 33(6): 737-742.
BOSWELL-CASTEEL R C, HAYS F A. Equilibrative nucleoside transporters-A review[J]. Nucleosides Nucleotides Nucleic Acids, 2017, 36(1): 7-30.
NAKAMURA K, SHINOZUKA K, YOSHIKAWA N. Anticancer and antimetastatic effects of cordycepin, an active component of Cordyceps sinensis[J]. J Pharmacol Sci, 2015, 127(1): 53-56.
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