Study on preparation and performance of quercetin nano⁃drug delivery system based on carboxymethyl chitosan

JI Lili; ZHANG Fuyuan; ZHENG Han; LI Shuangyan; YANG Jing; YU Peng

doi:10.16305/j.1007-1334.2023.2209077

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Study on preparation and performance of quercetin nano⁃drug delivery system based on carboxymethyl chitosan

更新时间：2023-03-21

- Study on preparation and performance of quercetin nano⁃drug delivery system based on carboxymethyl chitosan
- Shanghai Journal of Traditional Chinese Medicine Vol. 57, Issue 3, Pages: 72-79(2023)
- 作者机构：
  
  1.长春中医药大学药学院（吉林长春 130117）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2023.2209077
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汲丽丽,张釜源,郑韩,等.基于羧甲基壳聚糖的槲皮素纳米载药体系的制备和性能研究[J].上海中医药杂志,2023,57(3):72-79.

JI Lili,ZHANG Fuyuan,ZHENG Han,et al.Study on preparation and performance of quercetin nano⁃drug delivery system based on carboxymethyl chitosan[J].Shanghai Journal of Traditional Chinese Medicine,2023,57(3):72-79.
汲丽丽,张釜源,郑韩,等.基于羧甲基壳聚糖的槲皮素纳米载药体系的制备和性能研究[J].上海中医药杂志,2023,57(3):72-79. DOI： 10.16305/j.1007-1334.2023.2209077.

JI Lili,ZHANG Fuyuan,ZHENG Han,et al.Study on preparation and performance of quercetin nano⁃drug delivery system based on carboxymethyl chitosan[J].Shanghai Journal of Traditional Chinese Medicine,2023,57(3):72-79. DOI： 10.16305/j.1007-1334.2023.2209077.

摘要

目的,2,制备含锰二氧化硅载体，用其负载槲皮素并用羧甲基壳聚糖进行封堵，研究该纳米载药体系的结构和释药特征，拟合含锰二氧化硅纳米球（MMS）吸附槲皮素的热力学和动力学模型。,方法,2,以表面活性剂为模板合成含锰二氧化硅载体，采用兰缪尔（Langmuir）、弗伦德利希（Freundlich）和乔姆金（Temkin）等温吸附模型对含锰二氧化硅纳米载体吸附槲皮素热力学数据进行模型拟合，采用准一级动力学、准二级动力学和颗粒内扩散模型对吸附动力学数据进行模型拟合。采用透析袋法评价纳米载药体系体外释放情况。,结果,2,基于羧甲基壳聚糖的槲皮素纳米载药体系被成功制备，含锰二氧化硅载体对槲皮素的热力学和动力学吸附按照25 ℃>35 ℃>45 ℃的顺序减小，热力学拟合最佳模型是Freundlich等温吸附模型，动力学拟合最佳模型是准二级动力学模型。在pH6.5时，纳米载药体系在50 h内累计释放达92.53%，高于其他pH值（5.5，7.4）下的累计释放量，这种影响有利于治疗肿瘤。,结论,2,含锰二氧化硅载体吸附槲皮素符合Freundlich等温吸附模型和准二级动力学模型。温度对载体吸附槲皮素有影响，且环境pH值影响纳米载药体系中槲皮素的释放。

Abstract

Objective,2,The manganese-containing silica carrier was prepared， which was loaded with quercetin and blocked with carboxymethyl chitosan. The structure and release characteristics of the nano-drug loading system were studied， and the thermodynamic and kinetic model of quercetin adsorption by manganese-containing silica nanoparticles was fitted.,Methods,2,Manganese-containing silica carriers were synthesized using surfactant as template. Langmuir， Freundlich and Temkin isothermal adsorption models were used to fit the thermodynamic data of quercetin adsorption on manganese-containing silica nanoparticles. Quasi-first-order kinetics， quasi-second-order kinetics and intra-particle diffusion model were used to fit the adsorption kinetic data. The ,in vitro, release of nano-drug loading system was evaluated by dialysis bag method.,Results,2,The nano-drug loading system of quercetin based on carboxymethyl chitosan was successfully prepared. The thermodynamic and kinetic adsorption of quercetin on manganese-containing silica carriers decreased in the order of 25 ℃>35 ℃>45 ℃. The best thermodynamic fitting model was Freundlich isothermal adsorption model， and the best kinetic fitting model was quasi-second-order kinetic model. In pH6.5， the cumulative release of nano-drug loading system reached 92.53% within 50 hours， which was higher than that at other pH values （5.5 and 7.4）. This effect was beneficial to the treatment of tumors.,Conclusion,2,The adsorption of quercetin on manganese-containing silica carrier corresponds with Freundlich isothermal model and quasi-second-order kinetic model. The temperature has an effect on the adsorption of quercetin on the carrier， and the environmental pH value affects the release of quercetin in the nano-drug loading system.

关键词

槲皮素羧甲基壳聚糖肿瘤纳米载药体系化学治疗中药研究

Keywords

quercetincarboxymethyl chitosantumornano-drug loading systemchemotherapytraditional Chinese herbal medicine research

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