Objective,2,To provide an objective basis for visual diagnosis theory of traditional Chinese medicine （TCM） and classification of liver disease syndromes and symptoms by analyzing the changes of fundus vascular images with the machine learning method in patients with non-alcoholic fatty liver disease （NAFLD） of common TCM syndromes （internal accumulation of damp-heat syndrome， liver depression and spleen deficiency syndrome）.,Methods,2,General data such as gender and age， as well as clinical symptoms were collected from NAFLD patients and healthy controls， and their fundus vascular images were collected with professional fundus cameras. The fundus vessels were segmented by the improved U-Net model， and the color， morphology and Haralick texture features were extracted by computer vision technology. A decision tree model was used to develop a disease prediction model with healthy people as controls. The fundus image features of NAFLD patients with internal accumulation of damp-heat syndrome and liver depression spleen deficiency syndrome， as well as the correlation between the fundus image features and influencing factors such as general data， clinical symptoms and so on were analyzed by SPSS 26.0 and R language.,Results,2,①A total of 126 NAFLD patients with different syndromes were included （61 cases with internal accumulation of damp-heat syndrome， 44 cases with liver depression and spleen deficiency syndrome， and 21 cases with nontypical syndrome）， and were divided into different groups accordingly. The healthy group included 110 healthy individuals as controls. ②The diagnosis model of NAFLD disease was established based on the decision tree model， with an accuracy of 0.972， a sensitivity of 0.970， a specificity of 0.975， and an AUC of 0.995， which can be effectively used for the analysis of fundus vascular features of NAFLD patients. ③In terms of vessel morphology and color， the internal accumulation of damp-heat syndrome group and liver depression and spleen deficiency syndrome group had smaller area， and higher small rate， curved and small curved than the nontypical syndrome group （,P,<,0.05）. In terms of vascular texture， the internal accumulation of damp-heat syndrome group had higher value of asm and lower value of den than the nontypical syndrome group （,P,<,0.05）， and the liver depression and spleen deficiency syndrome group had lower values of dva and den， and higher value of idm than the nontypical syndrome group （,P,<,0.05）. ④A total of 6 common symptoms of NAFLD were obtained by screening， which were fatigue， dry mouth， lack of sleep at night， thin and unformed stools， dry eyes and sleepiness and desire to sleep during the day. ⑤The results of correlation analysis showed that the age of NAFLD patients was positively correlated with asm， curved and idm （,P,<,0.05）， and negatively correlated with area， width， bsd， den， cor， ent， sen and f13 （,P,<,0.05）， and gender was negatively correlated with small curved （,P,<,0.05）. Dry eyes were negatively correlated with area， width and f13 （,P,<,0.05）； thin and unformed stools were positively correlated with curved， con and dva （,P,<,0.05）， and negatively correlated with idm （,P,<,0.05）.,Conclusion,2,The presence of specific changes in the vascular texture of fundus images of NAFLD patients with internal accumulation of damp-heat syndrome and liver depression and spleen deficiency syndrome may provide data support for the study of the TCM syndrome and symptom classification of NAFLD based on fundus vascular features.