Chemical constituents and rapid nondestructive identification of mountain⁃cultivated ginseng， cultivated ginseng， red ginseng and American ginseng powder

LI Ling; JIANG Meifang; ZHAO Linsong; ZHANG Yanxin; QIN Chaoyi; CHEN Danqing; GAO Qi

doi:10.16305/j.1007-1334.2023.2207001

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Chemical constituents and rapid nondestructive identification of mountain⁃cultivated ginseng， cultivated ginseng， red ginseng and American ginseng powder

更新时间：2023-01-07

- Chemical constituents and rapid nondestructive identification of mountain⁃cultivated ginseng， cultivated ginseng， red ginseng and American ginseng powder
- Shanghai Journal of Traditional Chinese Medicine Vol. 57, Issue 1, Pages: 76-86(2023)
- 作者机构：
  
  1.上海中医药大学（上海 201203）
  2.上海上药杏灵科技药业股份有限公司（上海 201703）
  3.上海上药神象健康药业有限公司（上海 200336）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2023.2207001
  CLC：
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李玲,江美芳,赵林松,等.林下山参、园参、红参与西洋参粉末的化学成分及快速无损鉴别[J].上海中医药杂志,2023,57(1):76-86.

LI Ling,JIANG Meifang,ZHAO Linsong,et al.Chemical constituents and rapid nondestructive identification of mountain⁃cultivated ginseng， cultivated ginseng， red ginseng and American ginseng powder[J].Shanghai Journal of Traditional Chinese Medicine,2023,57(1):76-86.
李玲,江美芳,赵林松,等.林下山参、园参、红参与西洋参粉末的化学成分及快速无损鉴别[J].上海中医药杂志,2023,57(1):76-86. DOI： 10.16305/j.1007-1334.2023.2207001.

LI Ling,JIANG Meifang,ZHAO Linsong,et al.Chemical constituents and rapid nondestructive identification of mountain⁃cultivated ginseng， cultivated ginseng， red ginseng and American ginseng powder[J].Shanghai Journal of Traditional Chinese Medicine,2023,57(1):76-86. DOI： 10.16305/j.1007-1334.2023.2207001.

摘要

目的,2,基于超高效液相色谱-四级杆-飞行时间串联质谱（UPLC-Q-TOF-MS）结合多元统计学方法，筛选林下山参、园参、红参与西洋参的主要差异性成分；基于近红外光谱（NIRS）技术与多元统计学方法，建立不同参品的快速无损鉴别方法。,方法,2,采用Waters Acquity UPLC BEH C18色谱柱，流动相为0.1%甲酸水-乙腈，梯度洗脱，在负离子模式下采集质谱数据，通过与文献数据及对照品比对，鉴定林下山参、园参、红参与西洋参中人参皂苷类成分；通过正交偏最小二乘判别分析（OPLS-DA），以变量重要性投影（VIP）值>1为标准筛选4种参的主要差异性成分；采集4种参粉末的NIRS，采用光谱预处理方法扣除仪器噪声及背景干扰，通过OPLS-DA，构建4种参粉末的快速无损判别模型。,结果,2,共鉴定4种参中54种人参皂苷类成分，筛选了18个主要差异性成分；NIRS技术采用二阶导数预处理方法结合OPLS-DA可实现4种参种类判别分析。,结论,2,本研究阐明了林下山参、园参、红参与西洋参的物质基础及差异性成分，可为不同参品的质量标志物的确定提供参考；同时建立了不同参品粉末的快速无损鉴别方法，为生产及市场上不同参品的掺假鉴别提供了有力的技术支撑。

Abstract

Objective,2,To screen out the main differential components of mountain-cultivated ginseng， cultivated ginseng， red ginseng and American ginseng based on ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF-MS） and multivariate statistical methods， and to establish a rapid nondestructive method for identifying different varieties of ginseng based on near-infrared spectroscopy （NIRS） technology and multivariate statistical methods.,Methods,2,Mass spectrometry data was collected in negative ion mode using the Waters Acquity UPLC BEH C18 chromatographic column and gradient elution with a mobile phase of 0.1% formic acid in water and acetonitrile， and ginsenosides were identified in mountain-cultivated ginseng， cultivated ginseng， red ginseng and American ginseng by comparing with literature data and reference substances. The main differential components of the four kinds of ginseng species were screened by orthogonal partial least squares discriminant analysis （OPLS-DA） with the variable importance projection （VIP） value greater than 1. The NIRS of the four kinds of ginseng species powders was collected， the instrument noise and background interference were deducted by spectral pre-processing method， and a rapid nondestructive discriminant model of the four kinds of ginseng species powders was constructed through OPLS-DA.,Results,2,A total of 54 ginsenoside components were identified in the four kinds of ginseng species， and 18 main differential components were screened out. The NIRS technology using the second-order derivative preprocessing method and OPLS-DA could help obtain the discriminant analysis of the four kinds of ginseng species.,Conclusions,2,The material basis and differential components of mountain-cultivated ginseng， cultivated ginseng， red ginseng and American ginseng have been elucidated， which provides a reference for the quality markers of different varieties of ginseng products. Meanwhile， a rapid nondestructive method for identifying different varieties of ginseng powders has been established， which provides a strong technical support for identifying adulteration of different ginsengs in the production and on the market.

关键词

林下山参园参红参西洋参差异性成分无损鉴别中药研究

Keywords

mountain-cultivated ginsengcultivated ginsengred ginsengAmerican ginsengdifferential componentsnondestructive identificationtraditional Chinese herbal medicine research

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