Differences of GABA and Glu <italic style="font-style: italic">in vivo</italic> in sleep deprived rats due to <italic style="font-style: italic">Zhi</italic> unrest in kidney or<italic style="font-style: italic"> Po</italic> unrest in lung

YAN Deqi; ZHANG Xingping; YAO Qianwei; ZHANG Wenhui; HE Yusong; CUI Rui; TONG Zhenpeng; WANG Kaikai; LIANG Ruining; CHEN Xu

doi:10.16305/j.1007-1334.2023.2205053

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Differences of GABA and Glu in vivo in sleep deprived rats due to Zhi unrest in kidney or Po unrest in lung

更新时间：2023-03-21

- Differences of GABA and Glu in vivo in sleep deprived rats due to Zhi unrest in kidney or Po unrest in lung
- Shanghai Journal of Traditional Chinese Medicine Vol. 57, Issue 3, Pages: 87-92(2023)
- 作者机构：
  
  1.新疆医科大学第四附属医院（新疆乌鲁木齐 830017）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2023.2205053
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闫德祺,张星平,么嵌巍,等.志不安于肾和魄不安于肺不寐大鼠体内γ⁃氨基丁酸和谷氨酸的差异研究[J].上海中医药杂志,2023,57(3):87-92.

YAN Deqi,ZHANG Xingping,YAO Qianwei,et al.Differences of GABA and Glu in vivo in sleep deprived rats due to Zhi unrest in kidney or Po unrest in lung[J].Shanghai Journal of Traditional Chinese Medicine,2023,57(3):87-92.
闫德祺,张星平,么嵌巍,等.志不安于肾和魄不安于肺不寐大鼠体内γ⁃氨基丁酸和谷氨酸的差异研究[J].上海中医药杂志,2023,57(3):87-92. DOI： 10.16305/j.1007-1334.2023.2205053.

YAN Deqi,ZHANG Xingping,YAO Qianwei,et al.Differences of GABA and Glu in vivo in sleep deprived rats due to Zhi unrest in kidney or Po unrest in lung[J].Shanghai Journal of Traditional Chinese Medicine,2023,57(3):87-92. DOI： 10.16305/j.1007-1334.2023.2205053.

摘要

目的,2,比较志不安于肾和魄不安于肺不寐大鼠血清和脑γ-氨基丁酸（GABA）及谷氨酸（Glu）水平的差异，为中医不寐五神分型诊断法鉴别魄不安于肺和志不安于肾不寐提供依据。,方法,2,将36只雌性SD大鼠随机分为志不安于肾组（肾组）、魄不安于肺组（肺组）和正常组。采用久坐湿地法制备志不安于肾不寐大鼠模型，采用狭长平台浅水环境法制备魄不安于肺不寐大鼠模型。以睡眠监测评价睡眠剥夺情况，以水迷宫实验评价学习记忆功能障碍，以酶联免疫吸附试验（ELISA）评价血清GABA、Glu及脑Glu水平，以免疫组化法评价不同脑区的GABA水平。,结果,2,与正常组比较，肾组和肺组睡眠时间均缩短，肾组觉醒次数减少，肺组觉醒次数增加。肾组表现为睡眠后期不能再次入睡致睡眠时间减少，肺组表现为上午和下午频繁觉醒致睡眠时间减少（,P,<,0.05）。肾组和肺组逃避潜伏期均延长，穿越平台次数均减少（,P,<,0.05）。肾组逃避潜伏期长于肺组，穿越平台次数少于肺组（,P,<,0.05）。肺组血清GABA和Glu水平显著降低，肾组血清和脑Glu水平显著降低，且显著低于肺组（,P,<,0.05）；肾组和肺组下丘脑、丘脑、压部b区、运动区等区域GABA水平均显著降低，肺组显著低于肾组（,P,<,0.05）；听觉区和海马杏仁移行区肺组GABA水平低于肾组，肾组海马杏仁移行区GABA水平高于正常组（,P,<,0.05）。,结论,2,志不安于肾和魄不安于肺不寐大鼠均存在着学习记忆功能障碍，但志不安于肾不寐大鼠较为严重；志不安于肾不寐大鼠以Glu水平下降为主，魄不安于肺不寐大鼠以GABA水平下降为主，说明中医不寐五神分型诊断法中志不安于肾和魄不安于肺不寐之间Glu、GABA等分子水平存在差异。

Abstract

Objective,2,To compare serum and brain Gamma-aminobutyric acid （GABA） as well as glutamic acid （Glu） in the sleep deprived rats due to ,Zhi, unrest in the kidney or ,Po, unrest in the lung， in order to provide reference for their classification and diagnosis of insomnia in traditional Chinese medicine based on five-spirit.,Methods,2,Thirty-six female Sprague Dawley （SD） rats were randomly divided into three groups： ,Zhi, unrest in the kidney group （kidney group）， ,Po, unrest in the lung group （lung group） and normal group. The sleep deprived model of ,Zhi, unrest in the kidney was prepared by long time sitting on the cold and wet sand in the rat cage. The model of ,Po, unrest in the lung was prepared by the shallow water environment in narrow and long platforms. The sleep was evaluated through sleep monitor and the learning and memory function was evaluated by Water Morris Maze test. The levels of serum GABA and Glu as well as brain Glu were detected by enzyme-linked immunosorbent assay （ELISA）， and the levels of GABA in different brain regions were detected by immunohistochemistry.,Results,2,Compared with the normal group， the sleep time was shortened in the 2 model groups. The arousal times increased in the lung group and decreased in the kidney group. The kidney group showed a decrease in sleep time due to inability to fall asleep again in the late stage of sleep， while the lung group showed a decrease in sleep time caused by frequent awakening in the morning and afternoon （,P,<,0.05）. The escape latency in the kidney group was longer and the number of times crossing the platform in the kidney group was less than those in the lung group （,P,<,0.05）. The levels of serum GABA and Glu decreased significantly in the lung group. The levels of serum and brain Glu decreased significantly in the kidney group and lower than that in the lung group. The levels of GABA in hypothalamus， thalamus， retrosplenial granular b cortex， motor cortex， etc.， decreased significantly in the two model groups with lower levels in the lung group（,P,<,0.05）. The level of GABA in auditory area and hippocampal amygdaloid transitional area in the lung group was lower than that in the kidney group， while the level of GABA in hippocampal amygdala transitional area in the kidney group was higher than that in the normal group（,P,<,0.05）.,Conclusions,2,There exists learning and memory dysfunction in the 2 types of insomnia model rats， which is worse in the rats,with, Zhi, unrest in the kidney. Insomnia rats with, Zhi ,unrest in the kidney have lower Glu level， while insomnia rats,with ,Po ,unrest in the lung have lower GABA level， which indicates that there are differences in Glu， GABA and other molecular levels between ,Zhi, unrest in the kidney and ,Po, unrest in the lung in the diagnosis of TCM insomnia based on five-spirit classification.

关键词

失眠五脏藏神γ-氨基丁酸谷氨酸大鼠模型中药研究

Keywords

insomniaFive Zangs Storing SpiritsGABAGlurat modeltraditional Chinese herbal medicine research

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Differences of GABA and Glu in vivo in sleep deprived rats due to Zhi unrest in kidney or Po unrest in lung

DOI：10.16305/j.1007-1334.2023.2205053

摘要

Abstract

关键词

Keywords

references