Objective,2,To observe the changes of urodynamics and contractility of isolated detrusor strips at different time points after electroacupuncture （EA） in rats with detrusor overactivity （DO）， and provide a theoretical guidance and basis for the temporal dynamics in the effectiveness of clinical acupuncture treatment.,Methods,2,One hundred and sixty Wistar female rats were randomly divided into control， model， EA and drug groups， each with 40 rats， and each group was further randomly divided into 4 subgroups （corresponding to different observation time points： 2 h， 24 h， 48 h and 72 h after the intervention）， 10 rats in each subgroup. Except for the control group， all rats were prepared into DO model by intraperitoneal injection of cyclophosphamide. On the basis of the model， the EA group was given EA at Ciliao （BL32） and Huiyang （BL35） points， and the drug group was administered with imatinib mesylate by gavage. Rats in the control， model and drug groups were bound and fixed for 20 min as those in the EA group， and rats in the control， model and EA groups were administered with drinking water of the same body mass by gavage as those in the drug group， once a day for 3 consecutive days. The effective bladder capacity， urinary storage time and voiding pressure of the rats were analyzed at each observation time point by urodynamic detection technique. Changes in contractility of the isolated detrusor strips of the bladder were observed in each group using the isolated detrusor traction test.,Results,2,A total of 124 rats were finally included in the statistical analysis. After modeling， the effective bladder capacity of rats was significantly smaller and urinary storage time was significantly shorter in the model group than those of rats in the control group （,P,<,0.05）. At the time points of 2 h， 24 h， 48 h and 72 h after intervention， the effective bladder capacity of rats was significantly larger and urinary storage time of rats was significantly longer in the EA group and the drug group than those of rats in the model group （,P,<,0.05）， but the differences between the EA group and the drug group were not statistically significant （,P,>,0.05）. At 24 h after intervention， the effective bladder capacity of rats was larger and urinary storage time of rats was longer in the EA group than those of rats observed at other three time points （,P,>,0.05）. The contraction of isolated detrusor strips of rats in the model group had lower amplitude than that in the control group， while the contraction frequency was higher than that in the control group （,P,<,0.05）. Compared with the contraction amplitude of detrusor strips of rats in the model group， the contraction amplitude in the EA group increased significantly at 24 h after intervention （,P,<,0.05）， and the increase continued until 72 h after intervention， but there was no increase in the contraction amplitude in the drug group. Both EA and drug groups could reduce the contraction frequency of bladder detrusor strips in DO model rats， and the effect was optimal at 24 h after intervention （,P,<,0.05）.,Conclusion,2,Electroacupuncture can effectively increase the effective bladder capacity and urinary storage time of DO rats， increase the contraction amplitude of isolated detrusor strips and stabilize the contraction frequency of detrusor strips. The effect of electroacupuncture lasts at least for 72 h after acupuncture， and the effect is optimal at 24 h after intervention.