HAN Xinyan,CHEN Ziyu,YUAN Jinfeng,et al.Artemisia annua tea extract attenuating DNCB⁃induced atopic dermatitis by restraining Th2 cell mediated inflammatory responses in BALB/c mice[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(12):73-78.
HAN Xinyan,CHEN Ziyu,YUAN Jinfeng,et al.Artemisia annua tea extract attenuating DNCB⁃induced atopic dermatitis by restraining Th2 cell mediated inflammatory responses in BALB/c mice[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(12):73-78. DOI: 10.16305/j.1007-1334.2022.2202083.
Artemisia annua tea extract attenuating DNCB⁃induced atopic dermatitis by restraining Th2 cell mediated inflammatory responses in BALB/c mice
Objective,2,To evaluate the effects of Artemisia annua tea extract (AT) on improving skin lesions in dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) in BALB/c mice and to explore the possible mechanism.,Methods,2,Thirty-two male BALB/c mice were divided into normal control group, model group, AT group and dexamethasone group, with 8 mice in each group. In addition to the normal control group, AD was induced by DNCB stimulation in each mouse. At the 7th day, the model animal was given intragastric administration of AT (3 g·kg,-1,) and dexamethasone(1.5 mg·kg,-1,)for 13 days continuously. The effects of the drug on AD mice skin lesions were evaluated by clinical skin score, ear thickness and ear weight indexes. The infiltration of eosinophils and mast cells in mouse skin tissue were detected by hematoxylin and eosin (HE) and toluidine blue (TB) staining. ,IL,-,13, level was detected by enzyme-linked immunosorbent assay (ELISA). The mRNA expressions of inflammatory genes such as ,IL,-,13,, thymic stromal lymphopoietin (,TSLP,), ,IL,-,17, and ,TNF,-,α, in ear tissues were detected by qPCR.,Results,2,①Compared with the normal control group, after repeated application of DNCB on the skin of the back and ear of mice, the skin of the model group mice showed erythema, swelling, fester,dryness, flakiness, and other manifestations. The epidermis of the mice was thickened, and the infiltration of eosinophils and mast cells was increased (,P,<,0.05). The serum IL-13 level was significantly increased (,P,<,0.05). The mRNA expressions of ,IL-13,, ,TSLP,, ,IL-17, and ,TNF-α, in ear tissue were significantly up-regulated (,P,<,0.05). ②Compared with the model group, the AD-like symptoms of BALB/c mice induced by DNCB were significantly alleviated by AT treatment. The skin dryness and flakiness, erythema, swelling and fester were significantly improved (,P<,0.05), ear thickening was significantly inhibited (,P<,0.05), and mast cells and eosinophilic infiltration were significantly decreased (,P<,0.05). At the same time, the serum IL-13 level was significantly decreased (,P<,0.05), and the mRNA expressions of ,IL-13,, ,TSLP,, ,IL-17, and ,TNF-α, in ear tissues were also significantly down-regulated (,P<,0.05).,Conclusion,2,AT may have a therapeutic effect on AD by inhibiting the expression of Th2-related inflammatory cytokines including IL-13, TSLP, IL-17 and TNF-α.
关键词
特应性皮炎湿疹苦草茶黄花蒿抗炎免疫调节小鼠模型中药研究
Keywords
atopic dermatitiseczemaArtemisia annua teaArtemisia annuaanti-inflammatoryimmunoregulationmice modeltraditional Chinese herbal medicine research
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