Establishment of UPLC fingerprint and determination of multi⁃index components of Rabdosiae Rubescentis Herba formula granules

Rui DANG; Dingbang AN; Xin SUN; Qi XIE; Huida GUAN; Xuemei CHENG; Changhong WANG

doi:10.16305/j.1007-1334.2022.2112108

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Establishment of UPLC fingerprint and determination of multi⁃index components of Rabdosiae Rubescentis Herba formula granules

更新时间：2022-08-12

- Establishment of UPLC fingerprint and determination of multi⁃index components of Rabdosiae Rubescentis Herba formula granules
- Shanghai Journal of Traditional Chinese Medicine Vol. 56, Issue 8, Pages: 90-95(2022)
- 作者机构：
  
  1.上海中医药大学中药研究所，上海市复方中药重点实验室，中药标准化教育部重点实验室，上海中药标准化研究中心（上海 201203）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2022.2112108
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党蕊,安定邦,孙鑫等.冬凌草配方颗粒超高效液相色谱指纹图谱及多指标成分含量测定方法的建立[J].上海中医药杂志,2022,56(08):90-95.

DANG Rui,AN Dingbang,SUN Xin,et al.Establishment of UPLC fingerprint and determination of multi⁃index components of Rabdosiae Rubescentis Herba formula granules[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(08):90-95.
党蕊,安定邦,孙鑫等.冬凌草配方颗粒超高效液相色谱指纹图谱及多指标成分含量测定方法的建立[J].上海中医药杂志,2022,56(08):90-95. DOI： 10.16305/j.1007-1334.2022.2112108.

DANG Rui,AN Dingbang,SUN Xin,et al.Establishment of UPLC fingerprint and determination of multi⁃index components of Rabdosiae Rubescentis Herba formula granules[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(08):90-95. DOI： 10.16305/j.1007-1334.2022.2112108.

摘要

目的,2,建立冬凌草配方颗粒的超高效液相色谱（UPLC）法指纹图谱，且对冬凌草配方颗粒中多成分含量进行测定。,方法,2,采用沃特世（Waters） ACQUITY UPLC HSS T3色谱柱（2.1 mm×100 mm，1.8 μm），以乙腈、0.1%甲酸水溶液为流动相进行梯度洗脱，流速0.4 mL·min,-1,，检测波长350 nm，柱温为30 ℃，进样量为3 μL。,结果,2,建立了10批冬凌草配方颗粒的指纹图谱，得到8个共有峰，并指认了4个成分峰，分别是咖啡酸、阿魏酸、迷迭香酸、胡麻素，相似度在0.999～1.000之间。4种指标成分在各自浓度范围内呈良好的线性关系（,R,2,=0.999 7～1.000 0），平均加样回收率为90.27%～110.64%，,RSD,为0.07%～2.16%。,结论,2,该法重复性好，稳定可行，符合指纹图谱的分析要求，同时测定4种指标成分的含量作为其质量控制方法。

Abstract

Objective,2,To establish an ultra high performance liquid chromatography （UPLC） fingerprint of Rabdosiae Rubescentis Herba formula granules and determine the multi-component contents as a quality control method.,Methods,2,Waters ACQUITY UPLC HSS T3 column （2.1 mm × 100 mm，1.8 μ m） was used with acetonitrile and 0.1% formic acid aqueous solution as mobile phase for gradient elution with a flow rate of 0.4 mL ·min,-1,. The detection wavelength was 350 nm， the column temperature was 30 ℃， and the injection volume was 3 μL.,Results,2,The fingerprints of 10 batches of Rabdosiae Rubescentis Herba formula granules were established， 8 common peaks were obtained， and 4 component peaks were identified， namely caffeic acid， ferulic acid， rosmarinic acid and pedalitin， with a similarity of 0.999～1.000. The four components showed a good linear relationship in their respective concentration range （,R,2,=0.999 7～1.000 0）. The average recoveries were between 90.27% and 110.64% with ,RSD, of 0.07% ～2.16%.,Conclusion,2,This method has good repeatability， stability and feasibility， meets the analysis requirements of fingerprint， and can determine four components at the same time， which provides a reference basis for establishing the quality control standard of Rabdosiae Rubescentis Herba formula granules.

关键词

冬凌草配方颗粒超高效液相色谱指纹图谱含量测定中药研究

Keywords

Rabdosiae Rubescentis Herba formula granulesUPLCfingerprintcontent determinationtraditional Chinese herbal medicine research

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