Objective,2,To observe the effect of electroacupuncture on the decomposition and synthesis of collagen in anterior vagina wall of rats with stress urinary incontinence （SUI）.,Methods,2,Totally 60 SD rats were randomly divided into sham operation group （,n,=10） and model group （,n,=50）. The sham operation group was treated by laparotomy， and the ovarian tissue was found and sutured directly without ovariectomy and vaginal dilatation； the model group was established by bilateral ovariectomy after vaginal dilatation， and the sneeze test was used to test the success of the model. The successful model rats were randomly divided into the model group， the electroacupuncture non-acupoint group （“acupuncture non-acupoint group”）， the electroacupuncture group and the blocker group， and the model group did not receive any treatment. The rats in the electroacupuncture group were treated with electroacupuncture at bilateral “Shenshu（BL23）” and “Huiyang（BL35）” points for 20 min each time for 3 days. The rats in the acupuncture non-acupoint group were treated with acupuncture at the control points of “Shenshu（BL23）” and “Huiyang（BL35）” （1 cm beside the two acupoints） and electroacupunctured for 20 min each time for 3 days， while those in the blocker group were given doxycycline hydrochloride intragastrically for 3 days. After treatment， urodynamic indexes were evaluated， and the mRNA and protein expression of total collagen content （Col-Ⅰ， Col-Ⅲ）， degradation link related factors （MMP-1， MMP-2， MMP-9， TIMP-1）， collagen synthesis link related factors （DCN， TGF-β） in anterior vagina wall were detected by PCR， Western blot and immunohistochemistry.,Results,2,① After modeling， the positive sneezing test indicated that the SUI model was successful. Compared with the sham operation group， the maximum bladder capacity and abdominal pressure leakage point pressure of the model rats were significantly lower （,P,<,0.05）. ② After treatment， compared with the sham operation group， the expression levels of ,Col,-,I,， ,Col,-,Ⅲ, mRNAs and proteins， ,TGF,-,β, mRNA， and ,TIMP,-,1, mRNA and protein increased（,P,<,0.05）， while the expression levels of ,MMP,-,1， MMP,-,2， MMP,-,9, mRNAs and proteins， and ,DCN, mRNA and protein decreased（,P,<,0.05）in the model group. ③ Compared with the model group， the leak point pressure and the maximum capacity of bladder in the electroacupuncture group and the blocker group were significantly higher than those in the model group （,P,<,0.05）. In the electroacupuncture group， the expressions of ,Col,-,I,， ,Col,-,Ⅲ, mRNAs and proteins， ,TIMP,-,1, mRNA and ,TGF,-,β, mRNA increased（,P,<,0.05）， while the expressions of ,MMP,-,1,， ,MMP,-,2, mRNAs and proteins， ,MMP,-,9, mRNA， ,DCN, mRNA and protein decreased（,P,<,0.05） in the anterior vaginal wall. Compared with the acupuncture non-acupoint group， the expression,of, Col,-,Ⅲ, mRNA and protein and ,TIMP,-,1, mRNA increased（,P,<,0.05）， while the expressions of ,MMP,-,1,， ,MMP,-,2, mRNAs and proteins， ,MMP,-,9, mRNA， and ,DCN, mRNA and protein decreased （,P,<,0.05）in electroacupuncture group.,Conclusion,2,In SUI rats， it is found that the collagen is lost， the collagen degradation is increased， and the collagen synthesis is decreased in the anterior vaginal wall. Electroacupuncture can inhibit the abnormal degradation of collagen， promote collagen synthesis， increase the total content of collagen in the anterior vaginal wall of SUI rats， and then achieve the purpose of treating SUI， and the curative effect of electroacupuncture at acupoints is better than that of electroacupuncture at non-acupoints.