Effect of Qinghua Granules on intestinal type 1 taste receptor and downstream signal factors in db/db diabetic mice

Zhenxiu LIU; Lijuan ZHANG; Yang GAO; Jie XU; Peng DAI; Xin JIN; Yi CHEN; Feng TAO

doi:10.16305/j.1007-1334.2022.2111054

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Effect of Qinghua Granules on intestinal type 1 taste receptor and downstream signal factors in db/db diabetic mice

更新时间：2022-07-13

- Effect of Qinghua Granules on intestinal type 1 taste receptor and downstream signal factors in db/db diabetic mice
- Shanghai Journal of Traditional Chinese Medicine Vol. 56, Issue 5, Pages: 68-73(2022)
- 作者机构：
  
  1.上海中医药大学附属市中医医院内分泌科（上海 200071）
  2.上海市松江区中心医院内分泌科（上海　　　　　201600）
  3.上海市松江区方塔中医医院内科（上海 201600）
  4.上海中医药大学附属曙光医院内分泌科（上海 201300）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2022.2111054
  CLC：
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刘珍秀,张丽娟,高旸等.清化颗粒对db/db糖尿病小鼠肠道1型味觉受体及其下游信号因子影响的研究[J].上海中医药杂志,2022,56(05):68-73.

LIU Zhenxiu,ZHANG Lijuan,GAO Yang,et al.Effect of Qinghua Granules on intestinal type 1 taste receptor and downstream signal factors in db/db diabetic mice[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(05):68-73.
刘珍秀,张丽娟,高旸等.清化颗粒对db/db糖尿病小鼠肠道1型味觉受体及其下游信号因子影响的研究[J].上海中医药杂志,2022,56(05):68-73. DOI： 10.16305/j.1007-1334.2022.2111054.

LIU Zhenxiu,ZHANG Lijuan,GAO Yang,et al.Effect of Qinghua Granules on intestinal type 1 taste receptor and downstream signal factors in db/db diabetic mice[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(05):68-73. DOI： 10.16305/j.1007-1334.2022.2111054.

摘要

目的,2,观察清化颗粒对db/db糖尿病小鼠肠道1型味觉受体及其下游信号因子的影响。,方法,2,将db/db小鼠分为模型组、阳性对照组、中药组，每日分别予等量0.9%NaCl溶液、果糖、清化颗粒灌胃，持续4周。ELISA法分析小鼠血清胰高血糖素样肽-1（GLP-1）和糖化血红蛋白（HbA1c）浓度；qRT-PCR、Western blot法分别检测小鼠回肠组织GLP-1、1型味觉受体亚型（TAS1R1、TAS1R2、TAS1R3）及其下游信号因子转导素α（α-gustducin）、磷脂酶Cβ2（PLCβ2）和瞬时感受器电位阳离子通道蛋白5（,TRPM5,）mRNA和蛋白表达；免疫组化技术检测空肠组织GLP-1蛋白水平，同时观察小鼠空腹血糖和体质量变化。,结果,2,与模型组比较，中药组可提高小鼠血清GLP-1的血清浓度、回肠组织,GLP,-,1, mRNA和蛋白表达，上调小鼠回肠组织,TAS1R2,及其下游信号因子,PLCβ2,、,TRPM5 ,mRNA和蛋白表达（,P,<,0.05），上调小鼠回肠组织,TAS1R1,及下游信号因子,α,-,gustducin, mRNA和蛋白水平（,P,<,0.05）。在GLP-1外周效应方面，与模型组比较，中药组小鼠空腹血糖、体质量上升幅度及HbA1c水平降低（,P,<,0.05）。,结论,2,清化颗粒能通过激活1型味觉受体通路，促进肠道L细胞合成并分泌GLP-1，从而发挥GLP-1调节血糖稳态和降低体质量的外周效应。

Abstract

Objective,2,To observe the effect of Qinghua Granules on intestinal type 1 taste receptor and its downstream signal factors in db/db diabetic mice.,Methods,2,The db/db mice were divided into the model group， the positive control group and Qinghua Granules group. The mice were given respectively the same amount of 0.9%NaCl solution， fructose and Qinghua Granules daily for 4 weeks. The concentrations of serum glucagon-like peptide-1 （GLP-1） and glycosylated hemoglobin （HbA1c） were analyzed by ELISA， and the expressions of GLP-1， type 1 taste receptor （TAS1R1， TAS1R2， TAS1R3） and its downstream signal factors α-gustducin， phospholipase Cβ2 （PLCβ2） and transient receptor potential cation channel protein 5 （,TRPM5,） mRNA and protein in ileal tissue of mice were detected by qRT-PCR and Western blot respectively. The level of GLP-1 protein in jejunal tissue was detected by immunohistochemical technique， and the changes of fasting blood glucose and body mass were observed at the same time.,Results,2,Compared with the model group， the Qinghua Granules group could increase the serum concentration of GLP-1， ,GLP,-,1, mRNA and protein in ileal tissue， up-regulate the expression of ,TAS1R2, and its downstream signal factors including ,PLCβ2,， ,TRPM5, mRNA and protein in mouse ileum （,P,<,0.05）， and up-regulate the levels of ,TAS1R1, and downstream signal factor, α⁃gustducin, mRNA and protein in ileal tissue of mice （,P,<,0.05）. In terms of the peripheral effect of GLP-1， compared with the model group， the fasting blood glucose， the increase of body mass and the level of HbA1c in the Qinghua Granules group were significantly lower than those in the model group （,P,<,0.05）,.,Conclusion,2,Qinghua Granules can promote intestinal L cells to synthesize and secrete GLP-1 by activating type 1 taste receptor pathway， thus exerting the peripheral effect of GLP-1 in regulating blood glucose homeostasis and reducing body mass.

关键词

清化颗粒2型糖尿病1型味觉受体胰高血糖素样肽-1db/db小鼠中药研究

Keywords

Qinghua Granulesdiabetes mellitus type 2type1 taste receptorglucagon-like peptide-1db/db micetraditional Chinese herbal medicine research

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