Effects of CO<sub>2</sub> laser stimulation at acupoint ST36 on behaviors and infralimbic cortex c⁃Fos expression in rat model of post⁃traumatic stress disorder

Yazhu YANG; Chuting WU; Siyue QIN; Jiehang LU; Huijuan MAO; Xiaoyi QU; Sheng LIU

doi:10.16305/j.1007-1334.2022.2111045

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Effects of CO₂ laser stimulation at acupoint ST36 on behaviors and infralimbic cortex c⁃Fos expression in rat model of post⁃traumatic stress disorder

更新时间：2022-07-13

- Effects of CO₂ laser stimulation at acupoint ST36 on behaviors and infralimbic cortex c⁃Fos expression in rat model of post⁃traumatic stress disorder
- Shanghai Journal of Traditional Chinese Medicine Vol. 56, Issue 6, Pages: 79-84(2022)
- 作者机构：
  
  1.上海中医药大学针推学院（上海 201203）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2022.2111045
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杨雅竹,吴楚婷,秦思月等.CO₂激光灸刺激足三里穴对创伤后应激障碍模型大鼠行为学及边缘下皮质c⁃Fos表达的影响[J].上海中医药杂志,2022,56(06):79-84.

YANG Yazhu,WU Chuting,QIN Siyue,et al.Effects of CO₂ laser stimulation at acupoint ST36 on behaviors and infralimbic cortex c⁃Fos expression in rat model of post⁃traumatic stress disorder[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(06):79-84.
杨雅竹,吴楚婷,秦思月等.CO₂激光灸刺激足三里穴对创伤后应激障碍模型大鼠行为学及边缘下皮质c⁃Fos表达的影响[J].上海中医药杂志,2022,56(06):79-84. DOI： 10.16305/j.1007-1334.2022.2111045.

YANG Yazhu,WU Chuting,QIN Siyue,et al.Effects of CO₂ laser stimulation at acupoint ST36 on behaviors and infralimbic cortex c⁃Fos expression in rat model of post⁃traumatic stress disorder[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(06):79-84. DOI： 10.16305/j.1007-1334.2022.2111045.

摘要

目的,2,观察CO,2,激光灸刺激“足三里”穴对创伤后应激障碍（PTSD）模型大鼠行为学及边缘下皮质c-Fos表达的影响。,方法,2,将48只健康雄性SD大鼠随机分为空白组、模型组、激光穴位照射组、激光非穴位照射组，每组12只。模型组、激光穴位照射组、激光非穴位照射组采用单一连续刺激（SPS）方法建模。激光穴位照射组和激光非穴位照射组予CO,2,激光灸每日照射双侧“足三里”穴和非穴点，连续干预7 d。第8天，各组大鼠进行旷场实验（OFT）和高架十字迷宫（EPM）实验，结束后测定各组大鼠血浆皮质酮水平，免疫组织化学法检测边缘下皮质c-Fos的表达，并对各组大鼠行为学变化与边缘下皮质c-Fos表达数量作相关性分析。,结果,2,与模型组和激光非穴位照射组比较，激光穴位照射组大鼠的旷场实验中央活动时间、中央路程，以及高架十字迷宫实验中央停留时间和开臂进入次数均显著性增加（,P,<,0.05）。与模型组和激光非穴位照射组比较，激光穴位照射组大鼠的血浆皮质酮水平显著性降低（,P,<,0.05）。与模型组比较，激光穴位照射组边缘下皮质c-Fos的表达明显增加（,P,<,0.05）。各组大鼠边缘下皮质神经元c-Fos表达数量与大鼠的行为学变化存在显著相关性（,P,<,0.01）。,结论,2,CO,2,激光灸刺激“足三里”穴能有效改善PTSD模型大鼠的焦虑样行为，其机制或与边缘下皮质神经元激活有关。

Abstract

Objective,2,To observe the effect of CO,2, laser moxibustion at Zusanli （ST36） on anxiety-like behaviors and infralimbic cortex （IL） c-Fos expression in a rat model of post-traumatic stress disorder （PTSD）.,Methods,2,Totally 48 healthy male SD rats were randomly divided into control group （,n,=12）， model group （,n,=12）， acupoint laser stimulation（LS） group （,n,=12） and non-acupoint laser stimulation（Sham LS） group （,n,=12）. The animal model in PTSD， LS and Sham LS groups was established by single-prolonged stress （SPS） method. CO,2, laser stimulation was applied to bilateral Zusanli （ST36） in the LS group or non-acupoint in the Sham LS group for 20 minutes， once daily for consecutive 7 days. On the 8th day， rats in each group were tested by open field test （OFT） and elevated plus maze （EPM）， after which the plasma corticosterone levels were measured and the IL c-Fos expression was detected by immunohistochemical techniques， and the correlation analysis of the behavioral changes and the amount of c-Fos expression in the infralimbic cortex of rats in each group was conducted.,Results,2,Relative to the group and the Sham LS group， LS group was associated with increased center area time spent both in the OFT and EPM， as so as the open arms in the EPM test （,P,<,0.05）. Compared with the group and the Sham LS group， the plasma corticosterone levels were obviously decreased in the LS group （,P,<,0.05）. Compared with the group， the expression of IL c-Fos in the LS group was significantly increased （,P,<, 0.05）， and a significant correlation was detected between the amount of c-Fos expression in IL cortical neurons and behavioral changes in rats （,P,<,0.01）.,Conclusion,2,CO,2, laser stimulation at Zusanli （ST36） can effectively reduce anxiety-like behaviors in PTSD rats， and its mechanism may be related to the activation of IL cortical neurons.

关键词

激光灸隔物灸足三里穴创伤后应激障碍焦虑症边缘下皮质大鼠模型

Keywords

laser moxibustionsandwiched moxibustionST36post-traumatic stress disorderanxietyinfralimbic cortexrats model

references

CÉNAT J M， BLAIS-ROCHETTE C， KOKOU-KPOLOU C K， et al. Prevalence of symptoms of depression， anxiety， insomnia， posttraumatic stress disorder， and psychological distress among populations affected by the COVID-19 pandemic： a systematic review and meta-analysis［J］. Psychiatry Res， 2021（295）： 113599.

LIN L， CHENG K， TAN M T， et al. Comparison of the effects of 10.6-μm infrared laser and traditional moxibustion in the treatment of knee osteoarthritis［J］. Lasers Med Sci， 2020， 35（4）： 823-832.

TAGUCHI R， SATO K， ADOMI S， et al. Acupuncture and laser acupuncture as treatments for emotional distress in infertile women in Japan［J］. Med Acupunct， 2019， 31（6）： 372-378.

张彦峰，韩雅迪，赵中亭，等. 针灸干预创伤后应激障碍选穴规律探讨［J］. 中国针灸，2016， 36（11）： 1229-1232.

HOU Y， CHEN M， WANG C， et al. Electroacupuncture attenuates anxiety-like behaviors in a rat model of post-traumatic stress disorder： the role of the ventromedial prefrontal cortex［J］. Front Neurosci， 2021（15）： 690159.

BAYER H， BERTOGLIOL J. Infralimbic cortex controls fear memory generalization and susceptibility to extinction during consolidation［J］. Sci Rep， 2020， 10（1）： 15827.

MOHAMMADI-FARANI A， TAGHADOSI M， RAZIEE S， et al. In vivo blockade of 5HT3 receptors in the infralimbic medial prefrontal cortex enhances fear extinction in a rat model of PTSD［J］. Iran J Basic Med Sci， 2021， 24（6）： 776-786.

BERRETTA S， PANTAZOPOULOS H， CALDERA M， et al. Infralimbic cortex activation increases c-Fos expression in intercalated neurons of the amygdala［J］. Neuroscience， 2005， 132（4）： 943-953.

IWAMOTO Y， MORINOBU S， TAKAHASHI T， et al. Single prolonged stress increases contextual freezing and the expression of glycine transporter 1 and vesicle-associated membrane protein 2 mRNA in the hippocampus of rats［J］. Prog Neuro-Psychopharmacol Biol Psychiatry， 2007， 31（3）：642-651.

LIU L， LIU H， HOU Y， et al. Temporal effect of electroacupuncture on anxiety-like behaviors and c-Fos expression in the anterior cingulate cortex in a rat model of post-traumatic stress disorder［J］. Neurosci Lett， 2019（711）： 134432.

OH J Y， KIM Y K， KIM S N， et al. Acupuncture modulates stress response by the mTOR signaling pathway in a rat post-traumatic stress disorder model［J］. Sci Rep， 2018， 8（1）： 11864.

KNOX D， NAULT T， HENDERSON C， et al. Glucocorticoid receptors and extinction retention deficits in the single prolonged stress model［J］. Neuroscience， 2012（223）： 163-173.

孙浩然，徐艳玲，李长江，等. 创伤后应激障碍的啮齿类动物模型研究进展［J］. 中国实验动物学报，2020， 28（2）： 254-259.

余曙光，徐斌. 实验针灸学［M］. 北京：人民卫生出版社，2012： 270-271.

FERGUSON S A， PAULE M G， HOLSON R R. Neonatal dexametha-sone on day 7 in rats causes behavioral alterations reflective of hippocampal，but not cerebellar，deficits［J］. Neurotoxicol Teratol， 2001， 23（1）： 57-69.

PAXINOS G， WATSON C. The rat brain in stereotaxic coordinates（6th Ed）［M］. Amsterdam： Elsevier Academic， 2007： 52.

刘阳，宁武凤，胡茂荣.创伤后应激障碍发病机制研究进展［J］.国际精神病学杂志，2020， 47（3）： 425-428.

YABUKI Y， FUKUNAGA K. Clinical therapeutic strategy and neuronal mechanism underlying post-traumatic stress disorder （PTSD）［J］. Inter J Mol Sci， 2019， 20（15）： 3614.

AMORIM D， AMADO J， BRITO I， et al. Acupuncture and electroacupuncture for anxiety disorders： a systematic review of the clinical research［J］. Complement Ther Clin Pract，2018（31）： 31-37.

YAN B， ZHU S， WANG Y， et al. Effect of acupuncture on chronic pain with depression： a systematic review［J/OL］. Evid Based Complement Alterna Med， 2020［2021-10-15］. https：//pubmed.ncbi.nlm.nih.gov/32714417/https://pubmed.ncbi.nlm.nih.gov/32714417/.

GRANT S， COLAIACO B， MOTALA A， et al. Acupuncture for the treatment of adults with posttraumatic stress disorder： a systematic review and meta-analysis［J］. J Trauma Dissociation， 2018， 19（1）： 39-58.

DING N， LI L， SONG K， et al. Efficacy and safety of acupuncture in treating post-traumatic stress disorder： A protocol for systematic review and meta-analysis［J］. Medicine （Baltimore）， 2020， 99（26）： e20700.

石杰.艾灸疗法对抑郁症患者神经功能及认知功能的影响［J］. 中国中医药科技， 2020， 27（1）： 3.

赵非一，段怡汝，燕海霞，等. 艾灸联合太极拳及Jacoson渐进式肌肉放松训练干预运动性失眠临床疗效评价［J］. 沈阳体育学院学报，2016， 35（5）： 75-80.

卢小叶，孙冰，文钱，等. 针灸治疗焦虑症的选穴规律探讨［J］. 上海针灸杂志，2018， 37（4）： 474-479.

YUN S J， PARK H J， YEOM M J， et al. Effect of electroacupuncture on the stress-induced changes in brain-derived neurotrophic factor expression in rat hippocampus［J］. Neurosci Lett， 2002， 318（2）： 85-88.

ESHKEVARI L， PERMAUL E， MULRONEY S E. Acupuncture blocks cold stress-induced increases in the hypothalamus-pituitary-adrenal axis in the rat［J］. J Endocrinol， 2013， 217（1）： 95-104.

吴凡，张海蒙，王丽祯，等. 二氧化碳激光灸影响膝骨关节炎患者生活质量的随机双盲对照［J］. 中国组织工程研究与临床康复，2011， 15（26）： 4885-4890.

HARNETT N G， GOODMAN A M， KNIGHT D C. PTSD-related neuroimaging abnormalities in brain function， structure， and biochemistry［J/OL］. Exp Neurol， 2020［2021-10-15］. https：//www.sciencedirect.com/science/article/pii/S001448862030162X？via%3Di-hubhttps://www.sciencedirect.com/science/article/pii/S001448862030162X?via%3Di-hub.

VIDAL-GONZALEZ I， VIDAL-GONZALEZ B， RAUCH S L， et al. Microstimulation reveals opposing influences of prelimbic and infralimbic cortex on the expression of conditioned fear［J］. Learn Mem， 2006， 13（6）： 728-733.

QUIRK G J， MUELLER D. Neural mechanisms of extinction learning and retrieval［J］. Neuropsychopharmacology， 2008， 33（1）： 56-72.

KAZANAKAYA L， IVASHKINA O， ANOKHIN K. Patterns of cellular overlap in expression of c-Fos and arc activity-regulated genes in the mouse brain regions during acquisition and retrieval of contextual conditioned fear［J］. FASEB J， 2021， 35（S1）： 03483.

LIBERZON I， KRSTOV M， YOUNG E A. Stress-restress： effects on ACTH and fast feedback［J］. Psychoneuroendocrinology，1997， 22（6）： 443-453.

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Department of Proctology， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Department of Traditional Chinese Medicine， Xuanqiao Community Health Service Center， Pudong New Area

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Effects of CO2 laser stimulation at acupoint ST36 on behaviors and infralimbic cortex c⁃Fos expression in rat model of post⁃traumatic stress disorder

DOI：10.16305/j.1007-1334.2022.2111045

摘要

Abstract

关键词

Keywords

references

Effects of CO₂ laser stimulation at acupoint ST36 on behaviors and infralimbic cortex c⁃Fos expression in rat model of post⁃traumatic stress disorder