Expression differences of orexin receptors in related organs of insomnia rats with lung storing no inferior spirit

Qianwei YAO; Xingping ZHANG; Deqi YAN; Kaikai WANG; Zhenpeng TONG; Ning DENG

doi:10.16305/j.1007-1334.2022.2108104

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Expression differences of orexin receptors in related organs of insomnia rats with lung storing no inferior spirit

更新时间：2022-09-26

- Expression differences of orexin receptors in related organs of insomnia rats with lung storing no inferior spirit
- Shanghai Journal of Traditional Chinese Medicine Vol. 56, Issue 1, Pages: 75-80(2022)
- 作者机构：
  
  1.新疆医科大学（新疆乌鲁木齐 830017）
  2.新疆医科大学附属中医医院（新疆乌鲁木齐 830099）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2022.2108104
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么嵌巍,张星平,闫德祺,等.肺不藏魄型失眠大鼠相关脏器中食欲素受体表达差异[J].上海中医药杂志,2022,56(1):75-80.

YAO Qianwei,ZHANG Xingping,YAN Deqi,et al.Expression differences of orexin receptors in related organs of insomnia rats with lung storing no inferior spirit[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(1):75-80.
么嵌巍,张星平,闫德祺,等.肺不藏魄型失眠大鼠相关脏器中食欲素受体表达差异[J].上海中医药杂志,2022,56(1):75-80. DOI： 10.16305/j.1007-1334.2022.2108104.

YAO Qianwei,ZHANG Xingping,YAN Deqi,et al.Expression differences of orexin receptors in related organs of insomnia rats with lung storing no inferior spirit[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(1):75-80. DOI： 10.16305/j.1007-1334.2022.2108104.

摘要

目的,2,探寻肺不藏魄型失眠大鼠与对照组大鼠相关脏器中（肺、心、肝、脾、肾、大肠、脑）食欲素受体的表达差异，以充实客观实验依据，丰富中医不寐五神分型诊断法的应用。,方法,2,随机将16只大鼠分为对照组与模型组，每组8只。通过小平台水环境法制作失眠大鼠模型，运用免疫组化法观察大鼠肝、心、脾、肺、肾、大肠及脑组织中食欲素受体（OX1,R,、OX2,R,）的表达。,结果,2,①模型组较对照组大鼠肺脏组织中OX2,R,表达上调，心脏组织中OX1,R,表达上调，脑组织中OX1,R,、OX2,R,表达均上调，差异有统计学意义（,P,＜0.01），脾组织中OX1,R,、OX2,R,的表达较对照组差异无统计学意义（,P,＞0.01）；②两组大鼠肝、肾、大肠组织中OX1,R,与OX2,R,均未见明显表达。,结论,2,失眠模型大鼠的脑组织中OX1,R,、OX2,R,表达均上调，肺脏组织中OX2,R,表达上调，心脏组织中OX1,R,表达上调；食欲素受体可能是肺不藏魄型失眠模型大鼠的特征性神经递质之一。

Abstract

Objective,2,The aim is to explore the expression differences of orexin receptors in related organs （lung， heart， liver， spleen， kidney， large intestine and brain） between insomnia rats with lung storing no inferior spirit and control rats， so as to enrich the objective experimental basis and the application of five emotion-based classification and diagnosis of insomnia in traditional Chinese medicine.,Methods,2,Sixteen rats were randomly divided into the control group and model group， 8 rats in each group. The insomnia rat model was made by small platform-water environment method. The expressions of orexin receptors （OX1,R, and OX2,R,） in liver， heart， spleen， lung， kidney， large intestine and brain were detected by immunohistochemistry.,Results,2,①Compared with the control group， the expressions of OX2,R, in lung tissue， OX1,R, in heart tissue and OX1,R ,and OX2,R, in brain tissue were significantly up-regulated in the model group， with statistically significant differences （,P,<,0.01）， but there were no statistically significant differences in the expressions of OX1,R ,and OX2,R ,in spleen tissue between the control group and model group （,P,>,0.01）. ②There were no significant expressions of OX1,R, and OX2,R, in liver， kidney and large intestine tissues between the two groups.,Conclusion,2,The expressions of OX1,R, and OX2,R, in brain tissue， OX2,R, in lung tissue and OX1,R, in heart tissue of insomnia model rats are up-regulated， and orexin receptors may be one of the characteristic neurotransmitters in insomnia rats with lung storing no inferior spirit.

关键词

中医不寐五神分型肺不藏魄型失眠食欲素受体睡眠剥夺模型大鼠

Keywords

five emotion-based classification of insomnia in traditional Chinese medicinelung storing no inferior spiritinsomniaorexin receptorsleep deprivationmodel rat

references

张鹏，李雁鹏，吴惠涓，等. 中国成人失眠诊断与治疗指南（2017版）［J］. 中华神经科杂志，2018， 51（5）： 324-335.

MORIN C M， BENCA R. Chronic insomnia［J］. Lancet， 2012， 379（9821）： 1129-1241.

中华医学会神经病学分会，中华医学会神经病学分会睡眠障碍学组，中华医学会神经病学分会神经心理与行为神经病学学组. 中国成人失眠伴抑郁焦虑诊治专家共识［J］. 中华神经科杂志，2020， 53（8）： 564-574.

秦景明. 症因脉治［M］.北京：人民卫生出版社，2006： 220.

王雄. 失眠与调治［M］. 北京：学苑出版社，2011： 44-53.

张星平，范秀芳，邓宁，等. 中医不寐五神分型诊断法［J］. 中医杂志，2011， 52（24）： 2092-2093.

BENEDITO M A， CAMARINI R. Rapid eye movement sleep deprivation induces an increase in acetylcholinesterase activity in discrete rat brain regions［J］. Braz J Med Biol Res， 2001， 34（1）： 103-109.

徐争光，张星平，陈俊逾，等.失眠症中医不寐五神分型肺不藏魄型与肾不藏志型PSG睡眠结构参数差异性研究［J］.中华中医药杂志，2018， 33（7）： 2795-2797.

SAKURAI T， AMEMIYA A， ISHII M， et al. Orexins and orexin receptors： a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior［J］. Cell， 1998， 92（4）： 573-585.

TSUJINO N， SAKURAI T. Orexin/hypocretin： a neuropeptide at the interface of sleep， energy homeostasis， and reward system［J］. Pharmacol Rev， 2009， 61（2）： 162-176.

SASAKI K， SUZUKI M， MIEDA M， et al. Pharmacogenetic modulation of orexin neurons alters sleep /wakefulness states in mice［J］. PLoS One， 2011， 6（5）： e20360.

CLARK J W， BRIAN M L， DRUMMOND S P A， et al. Effects of orexin receptor antagonism on human sleep architecture：A systematic review［J］. Sleep Med Rev， 2020（53）： 101332.

BOOF M L， DINGEMANSE J， LEDERER K， et al. Effect of the new dual orexin receptor antagonist daridorexant on nighttime respiratory function and sleep in patients with mild and moderate obstructive sleep apnea［J］. Sleep， 2021， 44（6）： zsaa275.

范秀芳，梁政亭，张星平. 中医不寐五神分型与四诊分型在五脏定位上的一致性比较［J］.中医药学报，2011， 39（6）： 47-49.

张星平，朱奇，梁政亭，等.依据单一症状的中医不寐病位诊断及其价值［J］. 中华中医药杂志，2013， 27（6）： 1666-1668.

张星平. 中医不寐五神分型PSG特征及睡眠相关因子分布规律研究［D］. 乌鲁木齐：新疆医科大学，2017.

SIFFERLEN T， BOSS C， EMMANUELLE C， et al. Novel pyrazolo-tetrahydropyridines as potent orexin receptor antagonists［J］. Bioorg Med Chem Lett， 2010， 20（5）： 1539-1542.

韩丹. Orexin-A通过Orexin 1 Receptor和Orexin 2 Receptor引起创伤后应激障碍大鼠记忆异常机制的研究［D］. 沈阳：中国医科大学，2020.

SAKURAI T. The neural circuit of orexin （hypocretin）： maintaining sleep and wakefulness［J］. Nat Rev Neurosci， 2007， 8（3）： 171-181.

MIEDA M， HASEGAWA E， Kisanuki Y Y， et al. Differential roles of orexin receptor-1 and -2 in the regulation of non-REM and REM sleep［J］. Neurosci Res， 2011， 31（17）： 6518-6526.

FURLONG T M， VIANNA D M L， LIU L， et al. Hypocretin/orexin contributes to the expression of some but not all forms of stress and arousal［J］. Eur J Neurosci， 2009， 30（8）： 1603-1614.

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