Effect of Ruanjian Qingmai Grain on angiogenesis in Flila⁃EGFP transgenic zebrafish

Song HONG; Liuliu SUN; Tongkai CAI; Wenyi CAI; Yongbing CAO; Yemin CAO; Chenglin JIA

doi:10.16305/j.1007-1334.2022.2108085

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Effect of Ruanjian Qingmai Grain on angiogenesis in Flila⁃EGFP transgenic zebrafish

更新时间：2022-07-13

- Effect of Ruanjian Qingmai Grain on angiogenesis in Flila⁃EGFP transgenic zebrafish
- Shanghai Journal of Traditional Chinese Medicine Vol. 56, Issue 5, Pages: 81-86(2022)
- 作者机构：
  
  1.上海中医药大学附属上海市中西医结合医院（上海 200082）
  2.上海市中西医结合脉管病研究所（上海　　　200082）
  3.中国药科大学（江苏南京 210009）
  4.上海迪亚凯特生物医药科技有限公司（上海 201203）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2022.2108085
  CLC：
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洪松,孙留留,蔡同凯等.软坚清脉颗粒对Flila⁃EGFP转基因斑马鱼血管生长的作用研究[J].上海中医药杂志,2022,56(05):81-86.

HONG Song,SUN Liuliu,CAI Tongkai,et al.Effect of Ruanjian Qingmai Grain on angiogenesis in Flila⁃EGFP transgenic zebrafish[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(05):81-86.
洪松,孙留留,蔡同凯等.软坚清脉颗粒对Flila⁃EGFP转基因斑马鱼血管生长的作用研究[J].上海中医药杂志,2022,56(05):81-86. DOI： 10.16305/j.1007-1334.2022.2108085.

HONG Song,SUN Liuliu,CAI Tongkai,et al.Effect of Ruanjian Qingmai Grain on angiogenesis in Flila⁃EGFP transgenic zebrafish[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(05):81-86. DOI： 10.16305/j.1007-1334.2022.2108085.

摘要

目的,2,探索不同给药模式下，软坚清脉颗粒（RJQM）对Flila-EGFP转基因斑马鱼（以下简称“斑马鱼”）血管生长的促进作用，为RJQM的临床应用提供实验依据。,方法,2,采用内皮细胞生长因子受体酪氨酸激酶抑制剂（VRI）构建斑马鱼血管损伤模型，分为正常组，模型组，RJQM低（0.025 g/L）、中（0.1 g/L）、高（0.4 g/L）剂量组，通过药物治疗、早期干预、提前预防3种不同给药模式，探索RJQM不同药物浓度对斑马鱼血管生长的促进作用。,结果,2,在药物治疗模式下，与模型组比较，RJQM高剂量组能显著促进斑马鱼节间血管（ISVs）的生成，差异有统计学意义（,P,<,0.001）。在早期干预模式下，与模型组比较，RJQM低、中、高剂量组均能显著促进斑马鱼ISVs的生成，差异有统计学意义（,P,<,0.01，,P,<,0.001）。在提前预防模式下，与模型组比较，RJQM低、中、高剂量组斑马鱼ISVs虽有增多趋势，但差异无统计学意义（,P,>,0.05）。,结论,2,RJQM在药物治疗、早期干预给药模式下，具有显著促进斑马鱼血管生成的作用。

Abstract

Objective,2,To explore the promoting effect of Ruanjian Qingmai Grain （RJQM） on the growth of Flila-EGFP transgenic zebrafish （Hereinafter referred to as zebrafish） vessels in different modes of administration in order to provide experimental data support for the clinical application of RJQM.,Methods,2,The vascular injury model of zebrafish was constructed by VEGFR tyrosine kinase inhibitor（VRI），and was randomly divided into RJQM low dosage （0.025 g/L）， RJQM medium dosage （0.1 g/L）， RJQM high dosage （0.4 g/L） treated groups. The promoting effect of different concentrations of RJQM in angiogenesis of zebrafish was studied through drug treatment， early intervention and pre-prevention.,Results,2,In the drug treatment mode， compared with the model group， the high dose of RJQM significantly promoted the growth of intersegmental blood vessels （ISVs） in zebrafish with statistical significance （,P,<,0.001）. In the early intervention mode， compared with the model group， the low， medium and high doses of RJQM all significantly promoted the growth of ISVs in zebrafish with statistical significance （,P,<,0.01，,P,<,0.001）； In the pre-prevention mode， compared with the model group， the low， medium and high doses of RJQM promoted the growth of ISVs in zebrafish， but there was no significance difference （,P,>,0.05）.,Conclusion,2,RJQM has a significant effect of promoting angiogenesis of zebrafish in the administration modes of drug treatment and early intervention.

关键词

软坚清脉颗粒动脉硬化闭塞症血管生长斑马鱼中药研究

Keywords

RuanJian Qingmai Grainarteriosclerosis obliteransangiogenesiszebrafishtraditional Chinese herbal medicine research

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Department of Otolaryngology， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

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