Mechanism of Canmei Formula against colorectal cancer based on transcriptomics

Cui GUO; Yimin XU; Xinyue HAN; Xiaoqiang LIU; Runnan XIE; Xiangxue MENG; Xiaoling FU

doi:10.16305/j.1007-1334.2022.2106093

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Mechanism of Canmei Formula against colorectal cancer based on transcriptomics

更新时间：2022-07-10

- Mechanism of Canmei Formula against colorectal cancer based on transcriptomics
- Shanghai Journal of Traditional Chinese Medicine Vol. 56, Issue 2, Pages: 54-60(2022)
- 作者机构：
  
  1.辽宁中医药大学研究生院（辽宁沈阳 110847）
  2.上海中医药大学附属岳阳中西医结合医院肿瘤二科（上海 200437）
  3.上海市静安区市北医院疼痛科（上海 200435）
  4.上海市宝山区庙行镇社区卫生服务中心（上海 200443）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2022.2106093
  CLC：
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郭翠,徐祎敏,韩馨悦等.基于转录组学探讨蚕梅方抗结直肠癌的作用机制[J].上海中医药杂志,2022,56(02):54-60.

GUO Cui,XU Yimin,HAN Xinyue,et al.Mechanism of Canmei Formula against colorectal cancer based on transcriptomics[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(02):54-60.
郭翠,徐祎敏,韩馨悦等.基于转录组学探讨蚕梅方抗结直肠癌的作用机制[J].上海中医药杂志,2022,56(02):54-60. DOI： 10.16305/j.1007-1334.2022.2106093.

GUO Cui,XU Yimin,HAN Xinyue,et al.Mechanism of Canmei Formula against colorectal cancer based on transcriptomics[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(02):54-60. DOI： 10.16305/j.1007-1334.2022.2106093.

摘要

目的,2,利用二代测序技术探讨蚕梅方在转录组学层面上抗结直肠癌活性的作用机制。,方法,2,将来源于《本草纲目》中治疗肠风的核心药物乌梅及僵蚕组方为蚕梅方，对RKO细胞（肠癌细胞）进行干扰，采用二代高通量测序平台（Illumina Hi-Seq）测序技术分别对对照组（RKO-control）和实验组（RKO-treatment）进行高通量转录组测序，并应用基因本体论（GO）和京都基因与基因组百科全书（KEGG）进行生物功能富集分析，通过分子对接方法验证活性成分与关键靶点的结合作用。,结果,2,差异基因数为2 516个，其中上调基因1 279 个，下调基因1 237个，筛选得到叉头框蛋白D1（FOXD1）、腓骨蛋白2（FBLN2）、低密度脂蛋白受体A类结构域2（LDLRAD2）三个与结直肠癌相关的核心差异基因。GO分析发现，差异基因主要涉及单体代谢过程、细胞周期过程以及对有机物质的调控等过程；KEGG 分析发现，差异表达基因主要涉及代谢途径、细胞周期和RNA转运等信号通路，分子对接验证了蚕梅方主要活性化合物与核心靶点有较高的结合活性。,结论,2,蚕梅方可能通过多靶点、多途径的作用方式，从基因的转录表达、细胞代谢和凋亡等方面发挥抗结直肠癌作用。

Abstract

Objective,2,To explore the mechanism of anti-colorectal cancer activity of Canmei Formula at the transcriptome level by second generation sequencing.,Methods,2,Mume Fructus and Bombyx Batryticatus， the core drugs for the treatment of intestine wind from the ,Compendium of Materia Medica,， were selected to compose of Canmei Formula and to interfere with RKO cells （intestinal cancer cells）. The control group（RKO-Control）and the experimental group （RKO-treatment）were sequenced by the second generation high-throughput sequencing platform （IlluminaHi-Seq）， and the biological function enrichment analysis was carried out by using gene ontology （GO） and Kyoto encyclopedia of genes and genomes （KEGG）. Molecular docking methods was used to verify the binding effect of active ingredients with key targets. ,Result,s The number of differential genes was 2 516， including 1 279 up-regulated genes and 1 237 down-regulated genes. Three core differential genes related to colorectal cancer， FOXD1， FBLN2 and LDLRAD2， were screened. GO analysis showed that the differential genes were mainly involved in single-organism metabolic process， cell cycle and response to organic substance， while KEGG analysis showed that differentially expressed genes were mainly involved in metabolic pathway， cell cycle and RNA transport. Molecular docking results preliminarily verified that the main active components of Canmei Formula had high affinity with core targets.,Conclusion,2,Canmei Formula may play a role in anti-colorectal cancer from the aspects of gene transcriptional expression， cell metabolism and apoptosis through multi-target and multi-pathway.

关键词

蚕梅方结直肠癌转录组学代谢通路高通量测序平台中药研究

Keywords

Canmei Formulacolorectal cancertranscriptomicsmetabolic pathwayhigh-throughput sequencing platformtraditional Chinese herbal medicine research

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