Effect of Feiyanning Prescription combined with gefitinib on proliferation of lung adenocarcinoma H1975 cells <italic style="font-style: italic">in vitro</italic>

Xupeng FANG; Dan FANG; Haibin DENG; Zhenye XU; Zhongqi WANG; Wan SU; Yue MA; Lifang WANG

doi:10.16305/j.1007-1334.2022.2106020

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Effect of Feiyanning Prescription combined with gefitinib on proliferation of lung adenocarcinoma H1975 cells in vitro

更新时间：2022-08-12

- Effect of Feiyanning Prescription combined with gefitinib on proliferation of lung adenocarcinoma H1975 cells in vitro
- Shanghai Journal of Traditional Chinese Medicine Vol. 56, Issue 8, Pages: 79-83(2022)
- 作者机构：
  
  1.上海中医药大学附属龙华医院肿瘤二科（上海 200032）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2022.2106020
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方旭鹏,方丹,邓海滨等.肺岩宁方联合吉非替尼对肺腺癌H1975细胞体外增殖的影响[J].上海中医药杂志,2022,56(08):79-83.

FANG Xupeng,FANG Dan,DENG Haibin,et al.Effect of Feiyanning Prescription combined with gefitinib on proliferation of lung adenocarcinoma H1975 cells in vitro[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(08):79-83.
方旭鹏,方丹,邓海滨等.肺岩宁方联合吉非替尼对肺腺癌H1975细胞体外增殖的影响[J].上海中医药杂志,2022,56(08):79-83. DOI： 10.16305/j.1007-1334.2022.2106020.

FANG Xupeng,FANG Dan,DENG Haibin,et al.Effect of Feiyanning Prescription combined with gefitinib on proliferation of lung adenocarcinoma H1975 cells in vitro[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(08):79-83. DOI： 10.16305/j.1007-1334.2022.2106020.

摘要

目的,2,观察吉非替尼耐药肺腺癌H1975细胞在肺岩宁方、吉非替尼作用下体外增殖情况，探讨肺岩宁方逆转吉非替尼耐药可能的机制。,方法,2,选取肺腺癌T790M突变耐药株H1975细胞，分为对照组、吉非替尼组、肺岩宁方组和联合干预组。CCK-8法检测各组细胞在不同时间点（0 h、24 h、48 h、72 h）的增殖率；Western blot检测EGFR-PI3K/Akt/mTOR信号通路相关蛋白表达的影响。,结果,2,CCK-8结果显示，肺岩宁方组对于H1975细胞在体外增殖呈剂量依赖性抑制，即随着药物浓度的提高而增加；联合干预组对于抑制细胞体外增殖效果较单药明显。Western blot显示，联合干预组可下调p-EGFR、p-Akt、p-mTOR的表达。,结论,2,肺岩宁方联合吉非替尼可抑制H1975 细胞的体外增殖，体外抑制效果较单药治疗明显；其延缓吉非替尼耐药机制可能与下调EGFR-PI3K /Akt/mTOR信号通路相关蛋白的表达有关。

Abstract

Objective,2,To observe the proliferation of lung adenocarcinoma H1975 cells resistant to gefitinib ,in vitro, under the action of Feiyanning Prescription （FYN） and gefitinib， and to explore the possible mechanism of FYN in reversing gefitinib resistance.,Methods,2,Lung adenocarcinoma T790M mutation-resistant strain H1975 cells were selected and divided into control group， gefitinib group， FYN group and combination group. CCK-8 assay was used to detect the proliferation rate of cells in each group at different time （0 h， 24 h， 48 h， and 72 h）； Western blot was used to detect the expression of proteins related to EGFR-PI3K/Akt/mTOR signal pathway.,Results,2,The results of CCK-8 showed that FYN inhibited the proliferation of H1975 cells ,in vitro, in a dose-dependent manner， that is， its inhibitory power increased with the increase of drug concentration， and the effect of combination drug on inhibiting cell proliferation ,in vitro, was more obvious than that of single drug. Western blot showed that FYN combined with gefitinib could down-regulate the expression of p-EGFR， p-Akt and p-mTOR.,Conclusion,2,FYN combined with gefitinib can inhibit the proliferation of H1975 cells ,in vitro,， and the inhibitory effect ,in vitro, is more significant than that of gefitinib. The mechanism of delaying gefitinib resistance may be related to the down-regulation of EGFR-PI3K/Akt/mTOR signal pathway related protein expression.

关键词

肺腺癌肺岩宁方吉非替尼H1975细胞体外增殖中药研究

Keywords

lung adenocarcinomaFeiyanning PrescriptiongefitinibH1975 cellsproliferation in vitrotraditional Chinese herbal medicine research

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Effect of Feiyanning Prescription combined with gefitinib on proliferation of lung adenocarcinoma H1975 cells in vitro

DOI：10.16305/j.1007-1334.2022.2106020

摘要

Abstract

关键词

Keywords

references