Qualitative and quantitative analysis of zeaxanthin dipalmitate in fruits of Lycii Fructus

Mengqin XIA; Yiping LIU; Rui WANG; Li YANG; Zhengtao WANG; Yanhong SHI

doi:10.16305/j.1007-1334.2022.2104142

您当前的位置：

首页 >

文章列表页 >

Qualitative and quantitative analysis of zeaxanthin dipalmitate in fruits of Lycii Fructus

更新时间：2022-07-13

- Qualitative and quantitative analysis of zeaxanthin dipalmitate in fruits of Lycii Fructus
- Shanghai Journal of Traditional Chinese Medicine Vol. 56, Issue 5, Pages: 87-92(2022)
- 作者机构：
  
  1.上海中医药大学中药学院（上海 201203）
  2.上海中医药大学交叉科学研究院（上海 201203）
  3.上海中医药大学中药研究所，中药标准化教育部重点实验室（上海 201203）
  4.上海中医药大学中医药国际标准化研究所（上海 201203）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2022.2104142
  CLC：
扫描看全文
夏梦琴,刘怡萍,王瑞等.枸杞子中玉米黄素双棕榈酸酯的定性与定量分析[J].上海中医药杂志,2022,56(05):87-92.

XIA Mengqin,LIU Yiping,WANG Rui,et al.Qualitative and quantitative analysis of zeaxanthin dipalmitate in fruits of Lycii Fructus[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(05):87-92.
夏梦琴,刘怡萍,王瑞等.枸杞子中玉米黄素双棕榈酸酯的定性与定量分析[J].上海中医药杂志,2022,56(05):87-92. DOI： 10.16305/j.1007-1334.2022.2104142.

XIA Mengqin,LIU Yiping,WANG Rui,et al.Qualitative and quantitative analysis of zeaxanthin dipalmitate in fruits of Lycii Fructus[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(05):87-92. DOI： 10.16305/j.1007-1334.2022.2104142.

摘要

目的,2,建立枸杞子中玉米黄素双棕榈酸酯（ZD）定性和定量分析方法。,方法,2,采用薄层色谱（TLC）法，以石油醚∶乙酸乙酯（9.5∶0.5，,V,/,V,）为展开剂，温度选择室温25 ℃，湿度影响忽略不计，日光和紫外光灯（365 nm）下检视，建立ZD的定性鉴别方法；采用超高效合相色谱/二极管阵列检测器（UPC,2,-PDA），色谱柱为 HSS C,18,（150 mm×3.0 mm，1.8 μm），CO,2,和助溶剂甲醇为流动相梯度洗脱，流速0.8 mL/min，进行ZD的含量测定。,结果,2,所建立的TLC方法斑点清晰，重复性好，可实现不同产地枸杞子中ZD的快速、简便鉴别。UPC,2,-PDA可实现绿色、高效地对该成分进行含量测定，ZD在12.5 g/mL～500.0 g/mL范围内线性关系良好（,R,2,=0.999 7），平均回收率92.40%，RSD为1.15%。26批不同产地枸杞子样品中，ZD含量范围为0.929 mg/g～3.210 mg/g，不同地区枸杞子中ZD含量差异较小。,结论,2,所建立的定性、定量方法专属性强，重复性好，可用于枸杞子质量控制，可为后续枸杞子质量标准提升研究提供科学依据。

Abstract

Objective,2,To establish a method for qualitative and quantitative analysis of zeaxanthin dipalmitate （ZD） in fruits of Lycii Fructus.,Methods,2,A qualitative identification method for ZD was established by thin layer chromatography （TLC） with petroleum ether： ethyl acetate at 9.5∶0.5 （,v,/,v,） as developing solvent； the temperature was set at room temperature 25 ℃， and the influence of humidity was ignored. The thin layer plates were examined under sunlight and ultraviolet light at 365 nm. ZD content was determined by ultra-performance convergence chromatography/photodiode array detector （UPC,2,-PDA） equipped with a HSS C,18, column （150 mm×3.0 mm， 1.8 μm）， and CO,2, and cosolvent methanol were used as mobile phase for gradient elution at a flow rate of 0.8 mL/min.,Results,2,The established TLC method can be used for rapid and simple identification of ZD in Lycii Fructus of different origins with clear spots and good repeatability. UPC,2,-PDA method achieved green and efficient content determination of this compound. The linear relationship of ZD was good in the range of 12.5 μg/mL~500 μg/mL， R,2, was 0.999 7， the average recovery was 92.4%， and RSD was 1.15%. In 26 batches of Lycii Fructus samples from different origins， the content of ZD was ranged from 0.929 mg/g to 3.21 mg/g， and there was no significant difference in the ZD content of Lycii Fructus from different origins.,Conclusion,2,The established qualitative and quantitative methods are specific and repeatable that can be used for quality control of Lycii Fructus and provide a scientific basis for the further study on the improvement of quality standard of Lycii Fructus.

关键词

枸杞子玉米黄素双棕榈酸酯薄层色谱超高效合相色谱/二极管阵列检测器中药研究

Keywords

Lycii Fructuszeaxanthin dipalmitateTLCUPC2-PDAtraditional Chinese herbal medicine research

references

国家药典委员会. 中华人民共和国药典（2020年版）［M］. 北京：中国医药科技出版社，2020.

付文昊，于梅. 枸杞子的研究进展［J］. 世界最新医学信息文摘，2017， 17（98）： 104.

殷涛，徐宁，卢恒，等. 不同产地枸杞子质量评价研究［J］. 时珍国医国药，2020， 31（7）： 1707-1709.

张启立，崔治家，杨毛毛，等. 基于HPLC指纹图谱的不同产地枸杞子的差异性评价［J］. 国际药学研究杂志，2019， 46（3）： 232-237.

周芹，吴玉梅. 亲水性液相色谱串联质谱法测定红甜菜中甜菜碱含量［J］. 中国农学通报，2019， 35（7）： 134-138.

罗青，米佳，冉林武，等. 枸杞类胡萝卜素研究进展［J］. 食品工业科技，2018， 39（24）： 331-335.

FERNÁNDEZ M L Á， BOITEUX J， ESPINO M， et al. Natural Deep Eutectic Solvents-Mediated Extractions： the way forward for sustainable analytical developments［J］. Anal Chim Acta，2018（1038）：1-10.

ABDUL HADI N M， NG M H， CHOO Y M， et al. Performance of choline-based deep eutectic solvents in the extraction of tocols from crude palm oil［J］. J Am Oil Chem Soc， 2015， 92（11-12）： 1709-1716.

肖佳，高昊，周正群，等. 枸杞属中枸杞红素类成分研究进展［J］. 科学通报，2017， 62（16）： 1691-1698.

JOHNSON E J. Role of lutein and zeaxanthin in visual and cognitive function throughout the lifespan［J］. Nutr Rev， 2014， 72（9）： 605-612.

LEUNG I， TSO M， LI W，et al. Absorption and tissue distribution of zeaxanthin and lutein in rhesus monkeys after taking Fructus lycii （Gou Ji Zi） extract［J］. Invest Ophthalmol vis sci， 2001， 42（2）： 466-471.

XIAO J， WANG J T， XING F Y， et al. Zeaxanthin dipalmitate therapeutically improves hepatic functions in an alcoholic fatty liver disease model through modulating MAPK pathway［J］. PLoS One， 2014，9（4）： e95214.

INBARAJ B S， LU H， HUNG C F， et al. Determination of carotenoids and their esters in fruits of Lycium barbarum Linnaeus by HPLC-DAD-APCI-MS［J］. J Pharm Biomed Ana， 2008， 47（4-5）： 812-818.

KARIOTI A， BERGONZI M C， VINCIERI F F， et al. Validated method for the analysis of goji berry， a rich source of zeaxanthin dipalmitate［J］. J Agric Food Chem， 2014， 62（52）： 12529-12535.

PENG Y， MA C， LI Y W， et al. Quantification of zeaxanthin dipalmitate and total carotenoids in lycium fruits （Fructus Lycii）［J］. Plant Foods Hum Nutr， 2005， 60（4）： 161-164.

周慧吉，彭博，李廷钊，等. 枸杞子中玉米黄素双棕榈酸酯及枸杞酸的测定［J］. 食品工业科技，2020， 42（12）： 294-299.

赵薪鑫，焦芳，孙国峰，等. 萱草花瓣中类胡萝卜素分析样品的制备及 UPCC-MS 检测方法研究［J］. 植物研究，2017， 37（6）： 926-932.

Views

296

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Xinqian Ganjie Decoction on lncRNA SNHG16， EGFR and MUC5AC mRNA expression in chronic rhinosinusitis mice

Mechanism of Yucan Granules improving podocyte damage and treating diabetic nephropathy

Mechanism of Huangqi Decoction against liver fibrosis by network pharmacological analysis combined with related cell experiment

Baicalin modulates gut microbiota and inhibits liver metastasis of colorectal cancer induced by high⁃fat diet

Quantitative analysis of notoginsenoside R1， ginsenoside Rg1 and ginsenoside Rb1 by HPLC and their intestinal mucosal permeability

Related Author

No data

Related Institution

Department of Otolaryngology， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Department of Vascular Surgery， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Department of Nephrology， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

School of Traditional Chinese Medicine， Shanghai University of Traditional Chinese Medicine

School of Integrative Medicine， Shanghai University of Traditional Chinese Medicine

⁰