Effect of Guanxinkang on expression of proteins related to MAPKs/NF⁃κB signal pathway in liver tissue of LDLR<sup>-/-</sup>mice with atherosclerosis

Yifan ZHANG; Ping LIU; Yiru WANG; Jie DING

doi:10.16305/j.1007-1334.2022.2102026

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Effect of Guanxinkang on expression of proteins related to MAPKs/NF⁃κB signal pathway in liver tissue of LDLR^-/-mice with atherosclerosis

更新时间：2022-07-09

- Effect of Guanxinkang on expression of proteins related to MAPKs/NF⁃κB signal pathway in liver tissue of LDLR^-/-mice with atherosclerosis
- Shanghai Journal of Traditional Chinese Medicine Vol. 56, Issue 4, Pages: 79-85(2022)
- 作者机构：
  
  1.上海中医药大学附属龙华医院心病科（上海　200032）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2022.2102026
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张一凡,刘萍,王怡茹等.冠心康对动脉粥样硬化LDLR^-/-小鼠肝组织MAPKs/NF⁃κB信号通路相关蛋白表达的影响[J].上海中医药杂志,2022,56(04):79-85.

ZHANG Yifan,LIU Ping,WANG Yiru,et al.Effect of Guanxinkang on expression of proteins related to MAPKs/NF⁃κB signal pathway in liver tissue of LDLR^-/-mice with atherosclerosis[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(04):79-85.
张一凡,刘萍,王怡茹等.冠心康对动脉粥样硬化LDLR^-/-小鼠肝组织MAPKs/NF⁃κB信号通路相关蛋白表达的影响[J].上海中医药杂志,2022,56(04):79-85. DOI： 10.16305/j.1007-1334.2022.2102026.

ZHANG Yifan,LIU Ping,WANG Yiru,et al.Effect of Guanxinkang on expression of proteins related to MAPKs/NF⁃κB signal pathway in liver tissue of LDLR^-/-mice with atherosclerosis[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(04):79-85. DOI： 10.16305/j.1007-1334.2022.2102026.

摘要

目的,2,观察冠心康对LDLR,-/-,小鼠肝组织MAPKs/NF-κB信号通路相关蛋白表达的影响。,方法,2,将24只雄性LDLR,-/-,小鼠随机分为模型组，冠心康低、中、高剂量组，以C57BL/6J小鼠为对照组。除对照组外，每组给予高脂饲料喂养12周。对照组、模型组每日给予200 μL 0.9%NaCl溶液灌胃，冠心康低、中、高剂量组分别每日给予7.24 g/kg、14.48 g/kg、28.96 g/kg的中药煎剂200 μL灌胃，共12周。采用生化检测方法检测小鼠血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、总胆固醇（TC）、三酰甘油（TG）水平；油红O染色法观察小鼠斑块面积；HE染色法观察小鼠肝组织结构；Western blot测定细胞外调节蛋白激酶（Erk1/2），分子量为38 kDa的促分裂素愿活化蛋白激酶（P38），c-Jun氨基末端激酶（JNK），核因子κB抑制蛋白（IκB），核因子κB蛋白表达（NF-κB）；RT-PCR、ELISA法检测白介素-1β（IL-1β）、白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α） mRNA及含量；免疫荧光法观察TNF-α表达。,结果,2,与模型组比较，冠心康各剂量组小鼠血清TC、TG、ALT、AST水平降低（,P,<,0.05），主动脉斑块面积减少，肝组织病理变化改善，肝组织Erk1/2，P38，JNK，IκB，NF-κB水平降低（,P,<,0.05），肝组织,IL,-,1β,、,IL,-,6,、,TNF,-,α, mRNA表达下调（,P,<,0.05），血清IL-1β、IL-6、TNF-α含量减少（,P,<,0.05），肝组织TNF-α表达减少（,P,<,0.05）。,结论,2,冠心康可通过抑制MAPKs/NF-κB信号通路，减轻LDLR,-/-,小鼠肝脏炎症，进而改善动脉粥样硬化。

Abstract

Objective,2,To observe the effect of Guanxinkang （GXK） on the expression of proteins related to MAPKs/NF-κB signal pathway in the liver tissue of LDLR,-/-, mice.,Methods,2,Twenty-four male LDLR,-/-, mice were randomly divided into model group， GXK low， medium and high dose groups， and C57BL/6J mice were used as the control group. Except for the control group， each group was given high-fat feed for 12 weeks. The control group and the model group were given 200 μL of 0.9% NaCl solution daily. The GXK low， medium and high dose groups were given 7.24 g/kg， 14.48 g/kg， 28.96 g/kg of Chinese medicine decoction， 200 μL daily for 12 weeks. The serum levels of TC， TG， ALT and AST were detected by biochemical assay； the plaque area was observed by oil red O staining； the liver tissue structure was observed by hematoxylin-eosin （HE） staining； the expressions of Erk1/2， P38， JNK， IκB， NF-κB proteins were determined by Western blot； the mRNA and contents of IL-1， IL-6 and TNF-α were detected by RT-PCR and ELISA respectively； and the expression of TNF-α was observed by immunofluorescence.,Results,2,Compared with the model group， the levels of serum TC， TG， ALT and AST were decreased in each dose group of GXK （,P,<,0.05）； the area of aortic plaques was reduced and pathological changes in the liver tissue were improved. And the phosphorylation level of Erk1/2， P38， JNK， IκB， and NF-κB protein in liver tissue was decreased in GXK groups （,P,<,0.05）. The expressions of IL-1β， IL-6， TNF-α mRNA in the liver tissue were down-regulated in GXK groups （,P,<,0.05）. The contents of IL-1β， IL-6 and TNF-α in serum were decreased （,P,<,0.05） and the expression of TNF- α in liver tissue was reduced.,Conclusion,2,GXK can reduce liver inflammation and improve atherosclerosis in LDLR,-/-, mice by inhibiting MAPKs/ NF-κB signal pathway.

关键词

冠心康动脉粥样硬化MAPKs/NF-kB信号通路炎症模型小鼠中药研究

Keywords

GuanxinkangatherosclerosisMAPKs/NF-κB signal pathwayinflammationmodel miceChinese herbal medicine research

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Effect of Guanxinkang on expression of proteins related to MAPKs/NF⁃κB signal pathway in liver tissue of LDLR-/-mice with atherosclerosis

DOI：10.16305/j.1007-1334.2022.2102026

摘要

Abstract

关键词

Keywords

references

Effect of Guanxinkang on expression of proteins related to MAPKs/NF⁃κB signal pathway in liver tissue of LDLR^-/-mice with atherosclerosis