Effect and mechanism of Wuzhuyu Formula on nausea and vomiting of modeling rats with Chemotherapy⁃induced Pica

Tian LI; Fei CHEN; Yechen WU; Xiaoting WANG; Weihong ZHANG

doi:10.16305/j.1007-1334.2022.2011049

您当前的位置：

首页 >

文章列表页 >

Effect and mechanism of Wuzhuyu Formula on nausea and vomiting of modeling rats with Chemotherapy⁃induced Pica

更新时间：2022-07-10

- Effect and mechanism of Wuzhuyu Formula on nausea and vomiting of modeling rats with Chemotherapy⁃induced Pica
- Shanghai Journal of Traditional Chinese Medicine Vol. 56, Issue 2, Pages: 61-66(2022)
- 作者机构：
  
  1.上海中医药大学附属曙光医院宝山分院乳腺外科（上海 201900）
  2.上海中医药大学附属曙光医院乳腺外科（上海 201203）
- 作者简介：
- 基金信息：
- DOI：10.16305/j.1007-1334.2022.2011049
  CLC：
扫描看全文
李甜,陈飞,吴叶晨等.吴茱萸汤对化疗性异食癖恶心呕吐模型大鼠干预效果与作用机制的初步研究[J].上海中医药杂志,2022,56(02):61-66.

LI Tian,CHEN Fei,WU Yechen,et al.Effect and mechanism of Wuzhuyu Formula on nausea and vomiting of modeling rats with Chemotherapy⁃induced Pica[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(02):61-66.
李甜,陈飞,吴叶晨等.吴茱萸汤对化疗性异食癖恶心呕吐模型大鼠干预效果与作用机制的初步研究[J].上海中医药杂志,2022,56(02):61-66. DOI： 10.16305/j.1007-1334.2022.2011049.

LI Tian,CHEN Fei,WU Yechen,et al.Effect and mechanism of Wuzhuyu Formula on nausea and vomiting of modeling rats with Chemotherapy⁃induced Pica[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(02):61-66. DOI： 10.16305/j.1007-1334.2022.2011049.

摘要

目的,2,研究不同剂量吴茱萸汤对顺铂诱导的大鼠化疗相关呕吐及肠道黏膜炎症的药效并初步探讨其作用机制。,方法,2,灌胃复方（吴茱萸汤）使用飞行时间高分辨质谱法对其活性成分进行定性。48只SD大鼠随机分为对照组、模型组、不同剂量吴茱萸汤组（5、10、20 mL/kg）以及阳性对照组（托烷司琼），每组8只。观察造模后1、3、5、7 d大鼠异嗜高岭土情况；液质联用方法定量检测各组大鼠血清中5-羟色胺（5-HT）的浓度；HE染色和免疫荧光观察大鼠结肠组织变化以及磷酸化组蛋白H2AX（γ-H2AX）、白细胞共同抗原（CD45）的表达。,结果,2,吴茱萸碱、吴茱萸次碱为吴茱萸汤的主要成分。治疗后，与模型组比较，吴茱萸汤高、中剂量组大鼠异嗜高岭土的量明显减少、血清5-HT浓度显著降低（,P,<,0.01）；病理形态学观察可见，吴茱萸汤高、中剂量组对大鼠结肠黏膜具有保护作用，并降低了γ-H2AX及CD45的表达（,P,<,0.01）。,结论,2,吴茱萸汤能治疗顺铂诱导的大鼠化疗后呕吐，并能减轻其肠道炎症反应，其作用机制可能与降低血清中5-HT的浓度，抑制肠道γ-H2AX及CD45的表达有关。

Abstract

Objective,2,Study the effect of different doses of Wuzhuyu Decoction on cisplatin induced chemotherapy-related vomiting and intestinal mucositis in rats， and further explore its mechanism.,Methods,2,The active components of Wuzhuyu Decoction were identified by time-of-flight high-resolution mass spectrometry. Totally 48 SD rats were randomly divided into the control group， the model group， the different concentrations of Wuzhuyu Decoction groups （5，10，20 mL/kg） and the positive control group（tolansetron group），with 8 rats in each group. The heterophilic kaolin rats was observed on the 1st， 3rd， 5th and 7th day after modeling. The concentration of serum 5-hydroxytryptamine （5-HT） rats in each group was quantified by liquid chromatography-mass spectrometry （LC/MS）， and the changes of colonic tissue and the expression of γ-H2AX and CD45 were observed by HE staining and immunofluorescence.,Results,2,Evodiamine and rutaecarpine are the main components of Wuzhuyu Decoction. After treatment， compared with the model group， the amount of heterophilic kaolin and the concentration of serum 5-HT in the high and medium dose groups of Wuzhuyu Decoction decreased significantly （,P,<,0.01）， and the pathomorphological observation showed that the high and medium dose groups of Wuzhuyu Decoction had protective effect on rat colonic mucosa and decreased the expression of γ-H2AX and CD45 （,P,<,0.01）.,Conclusion,2,Wuzhuyu Decoction can treat cisplatin-induced vomiting and reduce intestinal inflammation in rats， and its mechanism may be related to reducing the concentration of 5-HT in serum and inhibiting the expression of γ-H2AX and CD45 in intestinal tract.

关键词

吴茱萸汤化疗相关恶心呕吐5-羟色胺磷酸化组蛋白H2AX白细胞共同抗原模型大鼠中药研究

Keywords

Wuzhuyu Decoctionchemotherapy induced nausea and vomiting5-HTγ-H2AXCD45model rattraditional Chinese herbal medicine research

references

SHAPIRO C L. Highlights of recent findings on quality-of-life management for patients with cancer and their survivors［J］. JAMA Oncol， 2016， 2（11）： 1401-1402.

ZHANG Q Y， WANG F X， JIA K K， et al. Natural product interventions for chemotherapy and radiotherapy-induced side effects［J］. Front Pharmacol， 2018（9）： 1253.

HERRSTEDT J， SUMMERS Y， JORDAN K， et al. Amisulpride prevents nausea and vomiting associated with highly emetogenic chemotherapy： a randomised， double-blind， placebo-controlled， dose-ranging trial［J］. Support Care Cancer， 2019， 27（7）： 2699-2705.

QI L， LUO Q， ZHANG Y， et al. Advances in toxicological research of the anticancer drug cisplatin［J］. Chem Res Toxicol， 2019， 32（8）： 1469-1486.

BERGER M J， ETTINGER D S， ASTON J， et al. NCCN guidelines insights： antiemesis， version 2.2017［J］. J Natl Compr Canc Netw， 2017， 15（7）： 883-893.

DRANITSARIS G， MOLASSIOTIS A， CLEMONS M， et al. The development of a prediction tool to identify cancer patients at high risk for chemotherapy-induced nausea and vomiting［J］. Ann Oncol， 2017， 28（6）： 1260-1267.

许辉. 肿瘤化疗病人恶心、呕吐健康教育需求调查［J］. 护理研究，2017， 31（33）： 4278-4280.

RAZVI Y， CHAN S， MCFARLANE T， et al. ASCO， NCCN， MASCC/ESMO： a comparison of antiemetic guidelines for the treatment of chemotherapy-induced nausea and vomiting in adult patients［J］. Support Care Cancer， 2019， 27（1）： 87-95.

濮忠建，马小平，王亚军，等. 不同针灸方法治疗化疗所致恶心呕吐临床研究［J］. 中国中医药信息杂志，2017， 24（3）： 34-37.

宋宸宇. 针刺背俞穴治疗放化疗所致顽固性呃逆［J］.中西医结合心血管病电子杂志， 2019， 7（3）： 131-133.

张林英，林才志，陈漫，等. 加味香砂六君子汤联合5-HT3受体拮抗剂类止吐药治疗肿瘤化疗呕吐的系统评价［J］. 湖南中医杂志，2016， 32（1）： 147-152.

龚自坤，郑秋惠，梅凯雁. 肿瘤化疗后恶心呕吐的中医治疗研究进展［J］. 亚太传统医药，2020， 16（9）： 200-205.

郭琪，马乐，王妤函，等. 中医外治法治疗化疗后相关性恶心呕吐的临床研究进展［J］. 新疆中医药，2019， 37（5）： 79-81.

王洪杰，吕冠华. 吴茱萸汤临床应用［J］. 中医药临床杂志，2016， 28（9）： 1252-1254.

谢相智，许国磊，吴欣芳，等. 参赭培气合吴茱萸汤治疗肝胃虚寒型反流性食管炎的临床观察［J］. 世界中医药，2017， 12（6）： 1291-1293.

LIU H W， ZOU Y H， CAO K G， et al. Efficacy of modified Wuzhuyu Decoction Granule for migraine patients with cold and stasis obstructing meridian syndrome： a randomized， double-blind， placebo-controlled trial［J］. Chin J Integr Med， 2018， 24（6）： 409-414.

JOHNSTON K D， LU Z， RUDD J A. Looking beyond 5-HT3 receptors： a review of the wider role of serotonin in the pharmacology of nausea and vomiting［J］. Eur J Pharmacol， 2014（722）： 13-25.

AL KURY L T， MAHGOUB M， HOWARTH F C， et al. Natural negative allosteric modulators of 5-HT3 receptors［J］. Molecules， 2018， 23（12）： 3186.

SIMINO G P， MARRA L P， ANDRADE E I， et al. Efficacy， safety and effectiveness of ondansetron compared to other serotonin-3 receptor antagonists （5-HT3RAs） used to control chemotherapy-induced nausea and vomiting： systematic review and meta-analysis［J］. Expert Rev Clin Pharmacol， 2016， 9（9）： 1183-1194.

郭宇，苏晓兰，李依洁，等. 温脾健肾方对腹泻型肠易激综合征大鼠结肠黏膜5-羟色胺及其受体的影响［J］. 上海中医药杂志，2016， 50（10）： 86-91.

连建伟，李冀. 方剂学：案例版［M］. 北京：科学出版社， 2007.

聂克，马素起. 化疗性大鼠异食癖恶心呕吐模型建立的考察［C］//中国抗癌协会. 2007 医学前沿论坛暨第十届全国肿瘤药理与化疗学术会议论文集. 青岛：中国工程院医药卫生学部，中国抗癌协会抗癌药物专业委员会，中国药理学会肿瘤药理专业委员会.2007： 198.

ROILA F， HESKETH P J， HERRSTEDT J. Prevention of chemotherapy- and radiotherapy-induced emesis： results of the 2004 Perugia International Antiemetic Consensus Conference［J］. Ann Oncol， 2006， 17（1）： 20-28.

PARATHODUVIL A A， SISUPALAN A， REMA P L. Comparison of antiemetic effectiveness of palonosetron versus ondansetron in patients on cancer chemotherapy： a prospective observational study in South Indians［J］. J Clin Diagn Res， 2017， 11（5）： FC10-FC14.

廖天华，余昭胜，刘俊波，等. 对常见化疗药物的中药药性探析［J］. 广西中医药，2013， 36（2）： 40-41.

康辉，陈天朝，姬海南，等. 基于UPLC-MS的吴茱萸汤对虚寒呕吐大鼠血清代谢谱的影响［J］. 中草药，2020， 51（23）： 6006-6014.

姬海南，王朋倩，吴茵，等. 基于UHPLC-MS的吴茱萸汤影响虚寒呕吐大鼠尿液代谢谱的研究［J］. 中草药，2019， 50（18）： 4352-4363.

李文兰，姬海南，王朋倩，等. 虚寒呕吐模型的复制和吴茱萸汤的止呕作用研究［J］. 中华中医药学刊，2017， 35（5）： 1066-1069.

LI Y Q， YANG Y H， ZHANG G L， et al. RNA-Seq reveals inflammatory mechanisms of Xiao-Ban-Xia-Tang decoction to ameliorate cisplatin-induced emesis in a rat pica model［J/OL］. Biomed Pharmacother， 2020 ［2020-11-01］. https：//www.sciencedirect. com/science/article/pii/S0753332220308921？via%3Dihubhttps://www.sciencedirect.com/science/article/pii/S0753332220308921?via%3Dihub.

李君，陈永灿. 吴茱萸汤临床及实验研究进展［J］. 浙江中医杂志，2013， 48（7）： 537-539.

Views

531

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of polysaccharide of bran-fried Atractylodis Rhizoma on rats of spleen deficiency model

Effects of Xinqian Ganjie Decoction on lncRNA SNHG16， EGFR and MUC5AC mRNA expression in chronic rhinosinusitis mice

Mechanism of Yucan Granules improving podocyte damage and treating diabetic nephropathy

Mechanism of Huangqi Decoction against liver fibrosis by network pharmacological analysis combined with related cell experiment

Baicalin modulates gut microbiota and inhibits liver metastasis of colorectal cancer induced by high⁃fat diet

Related Author

No data

Related Institution

College of Pharmacy, Liaoning University of Chinese Medicine

Department of Otolaryngology， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Department of Vascular Surgery， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Department of Nephrology， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Institute of Interdisciplinary Integrative Medicine Research， Shanghai University of Traditional Chinese Medicine

⁰